Planctomycetes bacterium SRT547

Taxonomy: cellular organisms; Bacteria; PVC group; Planctomycetes; unclassified Planctomycetes

Average proteome isoelectric point is 7.09

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6642 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5S9F4D7|A0A5S9F4D7_9BACT Inositol-1-monophosphatase OS=Planctomycetes bacterium SRT547 OX=2596890 GN=UABAM_04059 PE=3 SV=1
MM1 pKa = 7.73KK2 pKa = 10.02IAIILGISCLIFIGCVTEE20 pKa = 4.48EE21 pKa = 4.29VHH23 pKa = 6.92HH24 pKa = 6.47EE25 pKa = 4.05PHH27 pKa = 6.38VDD29 pKa = 4.05PYY31 pKa = 11.05PSDD34 pKa = 4.67PYY36 pKa = 11.02PSDD39 pKa = 4.5PDD41 pKa = 3.98PNPYY45 pKa = 10.23PSDD48 pKa = 4.36PDD50 pKa = 3.9PNPYY54 pKa = 8.63PTDD57 pKa = 3.9PEE59 pKa = 4.58PYY61 pKa = 8.62PPHH64 pKa = 6.9NEE66 pKa = 4.17TPSEE70 pKa = 3.96QSGKK74 pKa = 10.43EE75 pKa = 3.87FFNEE79 pKa = 4.58DD80 pKa = 3.21NNSSYY85 pKa = 11.34KK86 pKa = 10.34EE87 pKa = 3.97DD88 pKa = 3.59NHH90 pKa = 5.93NHH92 pKa = 4.75NHH94 pKa = 5.94NSGNDD99 pKa = 3.46FEE101 pKa = 4.8QGNDD105 pKa = 3.49YY106 pKa = 11.13VDD108 pKa = 3.75NDD110 pKa = 3.47SSEE113 pKa = 4.38EE114 pKa = 3.94KK115 pKa = 9.93TADD118 pKa = 3.46DD119 pKa = 3.62WLGDD123 pKa = 3.65DD124 pKa = 4.03GRR126 pKa = 11.84VEE128 pKa = 4.25EE129 pKa = 4.55EE130 pKa = 4.36VSGKK134 pKa = 10.22DD135 pKa = 3.13AFDD138 pKa = 4.06NGSEE142 pKa = 4.36DD143 pKa = 3.5LSEE146 pKa = 4.17PAKK149 pKa = 10.91DD150 pKa = 4.11SGPTDD155 pKa = 4.91PIDD158 pKa = 5.31DD159 pKa = 4.03FMDD162 pKa = 4.53DD163 pKa = 3.38GRR165 pKa = 11.84VEE167 pKa = 4.3EE168 pKa = 4.67EE169 pKa = 3.97TSGDD173 pKa = 3.62DD174 pKa = 5.64FFDD177 pKa = 3.44SDD179 pKa = 4.22YY180 pKa = 11.95

Molecular weight: 20.12 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5S9F2K6|A0A5S9F2K6_9BACT Protease modulator HflK OS=Planctomycetes bacterium SRT547 OX=2596890 GN=UABAM_00817 PE=3 SV=1
MM1 pKa = 7.27KK2 pKa = 10.58VKK4 pKa = 9.21TRR6 pKa = 11.84KK7 pKa = 9.65SSLKK11 pKa = 9.3YY12 pKa = 9.48RR13 pKa = 11.84KK14 pKa = 8.35KK15 pKa = 9.21TGFLTRR21 pKa = 11.84MKK23 pKa = 10.01TRR25 pKa = 11.84GGRR28 pKa = 11.84AIISNQRR35 pKa = 11.84KK36 pKa = 8.79RR37 pKa = 11.84RR38 pKa = 11.84ANKK41 pKa = 10.05KK42 pKa = 8.59NN43 pKa = 3.16

Molecular weight: 5.12 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6642

0

6642

2884994

29

6224

434.4

49.5

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.529 ± 0.03

1.311 ± 0.013

5.182 ± 0.031

6.505 ± 0.033

4.678 ± 0.022

5.442 ± 0.031

2.421 ± 0.015

7.478 ± 0.024

7.935 ± 0.037

9.527 ± 0.033

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.25 ± 0.013

5.055 ± 0.024

3.539 ± 0.015

4.778 ± 0.024

4.508 ± 0.021

6.353 ± 0.022

5.11 ± 0.023

6.331 ± 0.025

1.196 ± 0.012

3.873 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski