Thalassospira sp. HJ

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodospirillales; Thalassospiraceae; Thalassospira; unclassified Thalassospira

Average proteome isoelectric point is 5.89

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3669 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0D8CFG0|A0A0D8CFG0_9PROT Cro/Cl family transcriptional regulator OS=Thalassospira sp. HJ OX=1616823 GN=UF64_09390 PE=4 SV=1
MM1 pKa = 7.46KK2 pKa = 10.18KK3 pKa = 9.81ILFASSALVAIAAAGTAQASEE24 pKa = 4.56PIKK27 pKa = 10.76LSVGGYY33 pKa = 6.95MEE35 pKa = 4.08QWAGFADD42 pKa = 3.88QDD44 pKa = 3.84QGDD47 pKa = 4.22RR48 pKa = 11.84KK49 pKa = 10.48NAFQSDD55 pKa = 4.11TEE57 pKa = 4.03IHH59 pKa = 6.45FKK61 pKa = 11.5GEE63 pKa = 3.99TTLDD67 pKa = 3.12NGIEE71 pKa = 4.02VGAVVEE77 pKa = 4.69LEE79 pKa = 4.52GEE81 pKa = 4.29TSADD85 pKa = 3.92QIDD88 pKa = 4.0EE89 pKa = 4.04QYY91 pKa = 11.25LYY93 pKa = 11.54VNGGFGQVRR102 pKa = 11.84LGKK105 pKa = 9.91DD106 pKa = 3.1DD107 pKa = 4.02SAADD111 pKa = 3.51SMGFIAPSVGPVGVNDD127 pKa = 3.8GDD129 pKa = 3.74LTNWVDD135 pKa = 3.82AYY137 pKa = 11.27LIDD140 pKa = 4.18TVPSSGDD147 pKa = 3.36QKK149 pKa = 10.83RR150 pKa = 11.84VTYY153 pKa = 6.9YY154 pKa = 9.71TPSISGFQVGVSYY167 pKa = 11.54ADD169 pKa = 4.47DD170 pKa = 5.03DD171 pKa = 4.61GSNNNDD177 pKa = 3.36TANDD181 pKa = 3.54GDD183 pKa = 4.53NIVSAGINYY192 pKa = 9.64QGEE195 pKa = 4.24FDD197 pKa = 3.9GVSVGVSATGEE208 pKa = 3.82NWGEE212 pKa = 4.13GNWYY216 pKa = 10.47GLGATVGFGNFVVGSSWGHH235 pKa = 6.9IEE237 pKa = 5.38DD238 pKa = 6.12DD239 pKa = 3.88YY240 pKa = 12.09AQDD243 pKa = 3.26EE244 pKa = 4.69GSRR247 pKa = 11.84TGGDD251 pKa = 2.77TDD253 pKa = 4.08AFDD256 pKa = 3.88IGVSYY261 pKa = 11.35AMDD264 pKa = 4.32AATVSLSYY272 pKa = 10.93GYY274 pKa = 10.91AEE276 pKa = 4.19YY277 pKa = 11.2GNRR280 pKa = 11.84DD281 pKa = 2.95VDD283 pKa = 4.16LTDD286 pKa = 5.5DD287 pKa = 4.87DD288 pKa = 4.83VDD290 pKa = 4.11LSGTGEE296 pKa = 4.03ISTVDD301 pKa = 3.43LGLSYY306 pKa = 10.78TLGAGVAWRR315 pKa = 11.84SSIFWFDD322 pKa = 4.18DD323 pKa = 3.64EE324 pKa = 5.26SDD326 pKa = 3.54NAAIADD332 pKa = 3.75NDD334 pKa = 3.73GFGAVTGLALTFF346 pKa = 4.31

Molecular weight: 36.23 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0D8CK23|A0A0D8CK23_9PROT Signal peptidase I OS=Thalassospira sp. HJ OX=1616823 GN=UF64_00745 PE=3 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.27QPSKK9 pKa = 9.73LVRR12 pKa = 11.84KK13 pKa = 8.95RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.64GFRR19 pKa = 11.84SRR21 pKa = 11.84SATVGGRR28 pKa = 11.84RR29 pKa = 11.84VLTARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.82GRR39 pKa = 11.84KK40 pKa = 8.91RR41 pKa = 11.84LSAA44 pKa = 3.96

Molecular weight: 5.14 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3669

0

3669

1179098

41

7388

321.4

35.13

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.718 ± 0.048

0.938 ± 0.011

6.466 ± 0.071

6.137 ± 0.039

3.924 ± 0.03

8.258 ± 0.085

2.078 ± 0.023

5.982 ± 0.027

4.144 ± 0.039

9.676 ± 0.047

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.803 ± 0.022

3.287 ± 0.029

4.548 ± 0.033

3.187 ± 0.03

5.852 ± 0.046

5.67 ± 0.033

5.39 ± 0.035

7.29 ± 0.035

1.231 ± 0.018

2.422 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski