Granulicella rosea

Taxonomy: cellular organisms; Bacteria; Acidobacteria; Acidobacteriia; Acidobacteriales; Acidobacteriaceae; Granulicella

Average proteome isoelectric point is 6.61

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4295 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A239IYM9|A0A239IYM9_9BACT Isoamylase OS=Granulicella rosea OX=474952 GN=SAMN05421770_103335 PE=3 SV=1
MM1 pKa = 7.68SLGILNNIAAIYY13 pKa = 8.99AQNNLNQTQASLQTTLNQLSSGSRR37 pKa = 11.84INSGADD43 pKa = 3.18DD44 pKa = 4.1AAGLAVVDD52 pKa = 4.15GLGANIAALTQSSQNATDD70 pKa = 5.57GIGLLQTADD79 pKa = 3.52GALSQVTNLLDD90 pKa = 3.64RR91 pKa = 11.84AVTLSTEE98 pKa = 4.17AANGTLNSNQVSSANQEE115 pKa = 3.94YY116 pKa = 10.9QNILNEE122 pKa = 4.19IGDD125 pKa = 3.72IGTTTNFNSNSVFSNIANTVFVSDD149 pKa = 4.03GTGSGANVYY158 pKa = 10.72SDD160 pKa = 3.04IVGVLSKK167 pKa = 11.47SNVGQSAPVGAGVALTSGTATTSSLTVGSSVTFSALTNYY206 pKa = 10.39NDD208 pKa = 4.33TIAGTLSVSVAGGTAINSTIATGTSLTDD236 pKa = 4.27AVNQLNDD243 pKa = 3.31QYY245 pKa = 11.53NADD248 pKa = 3.44GSGIVASIGSGNKK261 pKa = 9.45LVLTGPEE268 pKa = 3.99DD269 pKa = 4.41AEE271 pKa = 4.21NTTAGTNVLAVSTNTLEE288 pKa = 4.93DD289 pKa = 3.81YY290 pKa = 11.24APAAAGATAGAGIDD304 pKa = 3.88FTNSDD309 pKa = 4.02VSTLTQSSAQTVLTTVTSAIAGVAYY334 pKa = 10.03QRR336 pKa = 11.84GIIGADD342 pKa = 2.97INQLTAASNVASAEE356 pKa = 4.18SEE358 pKa = 4.15NLTSAQSSIQSTNYY372 pKa = 9.61GQATSDD378 pKa = 3.47LAKK381 pKa = 10.71YY382 pKa = 9.63QVLSQTGISALAQANSVQQEE402 pKa = 4.33VLKK405 pKa = 10.92LLQQ408 pKa = 3.71

Molecular weight: 40.65 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A239DWU5|A0A239DWU5_9BACT Cytidine deaminase OS=Granulicella rosea OX=474952 GN=SAMN05421770_101689 PE=3 SV=1
MM1 pKa = 8.0PKK3 pKa = 9.02RR4 pKa = 11.84TFQPNRR10 pKa = 11.84RR11 pKa = 11.84HH12 pKa = 5.69RR13 pKa = 11.84AKK15 pKa = 9.55THH17 pKa = 5.53GFLSRR22 pKa = 11.84MKK24 pKa = 8.98TKK26 pKa = 10.79AGAAVLNRR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84AKK38 pKa = 10.03GRR40 pKa = 11.84HH41 pKa = 5.72KK42 pKa = 10.29IAVSAGFRR50 pKa = 11.84DD51 pKa = 3.66

Molecular weight: 5.87 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4295

0

4295

1567266

28

2848

364.9

39.54

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.702 ± 0.05

0.851 ± 0.012

5.017 ± 0.03

5.173 ± 0.046

3.738 ± 0.022

8.249 ± 0.044

2.183 ± 0.019

4.848 ± 0.027

3.535 ± 0.034

9.963 ± 0.049

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.204 ± 0.021

3.325 ± 0.032

5.473 ± 0.029

3.698 ± 0.027

5.895 ± 0.049

6.253 ± 0.038

6.572 ± 0.061

7.227 ± 0.029

1.294 ± 0.016

2.8 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski