Amino acid dipepetide frequency for Clostridium phage phiCDHM13

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
2.392AlaAla: 2.392 ± 0.61
1.248AlaCys: 1.248 ± 0.407
1.976AlaAsp: 1.976 ± 0.496
4.575AlaGlu: 4.575 ± 0.659
1.768AlaPhe: 1.768 ± 0.375
3.743AlaGly: 3.743 ± 0.694
0.728AlaHis: 0.728 ± 0.235
5.199AlaIle: 5.199 ± 0.704
4.887AlaLys: 4.887 ± 0.804
4.679AlaLeu: 4.679 ± 0.649
1.144AlaMet: 1.144 ± 0.321
3.743AlaAsn: 3.743 ± 0.532
1.56AlaPro: 1.56 ± 0.512
1.144AlaGln: 1.144 ± 0.391
1.768AlaArg: 1.768 ± 0.49
3.535AlaSer: 3.535 ± 0.737
3.639AlaThr: 3.639 ± 0.706
3.015AlaVal: 3.015 ± 0.657
0.936AlaTrp: 0.936 ± 0.273
2.288AlaTyr: 2.288 ± 0.518
0.0AlaXaa: 0.0 ± 0.0
Cys
0.624CysAla: 0.624 ± 0.223
0.0CysCys: 0.0 ± 0.0
0.624CysAsp: 0.624 ± 0.268
1.04CysGlu: 1.04 ± 0.311
0.416CysPhe: 0.416 ± 0.216
1.144CysGly: 1.144 ± 0.488
0.104CysHis: 0.104 ± 0.105
0.832CysIle: 0.832 ± 0.28
1.664CysLys: 1.664 ± 0.384
0.832CysLeu: 0.832 ± 0.353
0.52CysMet: 0.52 ± 0.21
0.936CysAsn: 0.936 ± 0.293
0.312CysPro: 0.312 ± 0.172
0.104CysGln: 0.104 ± 0.1
0.312CysArg: 0.312 ± 0.198
0.624CysSer: 0.624 ± 0.289
0.312CysThr: 0.312 ± 0.195
0.416CysVal: 0.416 ± 0.195
0.312CysTrp: 0.312 ± 0.177
0.52CysTyr: 0.52 ± 0.237
0.0CysXaa: 0.0 ± 0.0
Asp
2.6AspAla: 2.6 ± 0.489
1.144AspCys: 1.144 ± 0.282
3.743AspAsp: 3.743 ± 0.592
6.343AspGlu: 6.343 ± 0.846
2.6AspPhe: 2.6 ± 0.45
3.223AspGly: 3.223 ± 0.685
0.208AspHis: 0.208 ± 0.131
6.967AspIle: 6.967 ± 0.833
6.135AspLys: 6.135 ± 0.754
5.719AspLeu: 5.719 ± 0.89
2.08AspMet: 2.08 ± 0.427
3.015AspAsn: 3.015 ± 0.466
1.248AspPro: 1.248 ± 0.383
0.728AspGln: 0.728 ± 0.271
2.184AspArg: 2.184 ± 0.477
4.055AspSer: 4.055 ± 0.71
2.08AspThr: 2.08 ± 0.579
3.119AspVal: 3.119 ± 0.612
0.0AspTrp: 0.0 ± 0.0
2.184AspTyr: 2.184 ± 0.436
0.0AspXaa: 0.0 ± 0.0
Glu
5.407GluAla: 5.407 ± 0.89
1.04GluCys: 1.04 ± 0.333
4.367GluAsp: 4.367 ± 0.542
9.462GluGlu: 9.462 ± 1.507
4.159GluPhe: 4.159 ± 0.501
4.991GluGly: 4.991 ± 0.702
1.872GluHis: 1.872 ± 0.412
9.046GluIle: 9.046 ± 1.142
9.878GluLys: 9.878 ± 1.165
9.254GluLeu: 9.254 ± 0.984
2.496GluMet: 2.496 ± 0.551
6.343GluAsn: 6.343 ± 0.874
0.936GluPro: 0.936 ± 0.321
2.912GluGln: 2.912 ± 0.394
2.392GluArg: 2.392 ± 0.465
3.119GluSer: 3.119 ± 0.511
4.783GluThr: 4.783 ± 0.633
5.927GluVal: 5.927 ± 0.747
0.832GluTrp: 0.832 ± 0.321
3.639GluTyr: 3.639 ± 0.653
0.0GluXaa: 0.0 ± 0.0
Phe
1.04PheAla: 1.04 ± 0.33
0.416PheCys: 0.416 ± 0.175
2.6PheAsp: 2.6 ± 0.401
3.743PheGlu: 3.743 ± 0.66
1.352PhePhe: 1.352 ± 0.324
3.015PheGly: 3.015 ± 0.553
0.312PheHis: 0.312 ± 0.172
4.159PheIle: 4.159 ± 0.622
4.263PheLys: 4.263 ± 0.574
3.327PheLeu: 3.327 ± 0.518
1.04PheMet: 1.04 ± 0.321
3.639PheAsn: 3.639 ± 0.515
1.04PhePro: 1.04 ± 0.407
0.936PheGln: 0.936 ± 0.264
1.248PheArg: 1.248 ± 0.358
2.184PheSer: 2.184 ± 0.497
2.08PheThr: 2.08 ± 0.376
1.768PheVal: 1.768 ± 0.408
0.104PheTrp: 0.104 ± 0.122
1.04PheTyr: 1.04 ± 0.315
0.0PheXaa: 0.0 ± 0.0
Gly
3.847GlyAla: 3.847 ± 0.92
1.04GlyCys: 1.04 ± 0.335
3.015GlyAsp: 3.015 ± 0.754
4.991GlyGlu: 4.991 ± 0.665
2.704GlyPhe: 2.704 ± 0.632
5.303GlyGly: 5.303 ± 1.815
0.416GlyHis: 0.416 ± 0.259
4.679GlyIle: 4.679 ± 0.7
6.863GlyLys: 6.863 ± 0.803
4.471GlyLeu: 4.471 ± 0.736
1.768GlyMet: 1.768 ± 0.593
3.015GlyAsn: 3.015 ± 0.426
0.416GlyPro: 0.416 ± 0.24
0.832GlyGln: 0.832 ± 0.288
1.872GlyArg: 1.872 ± 0.558
2.184GlySer: 2.184 ± 0.507
3.119GlyThr: 3.119 ± 0.731
3.743GlyVal: 3.743 ± 0.581
0.832GlyTrp: 0.832 ± 0.303
2.704GlyTyr: 2.704 ± 0.696
0.0GlyXaa: 0.0 ± 0.0
His
0.52HisAla: 0.52 ± 0.212
0.0HisCys: 0.0 ± 0.0
0.52HisAsp: 0.52 ± 0.216
1.04HisGlu: 1.04 ± 0.379
0.624HisPhe: 0.624 ± 0.219
0.312HisGly: 0.312 ± 0.171
0.208HisHis: 0.208 ± 0.155
0.936HisIle: 0.936 ± 0.232
1.04HisLys: 1.04 ± 0.288
0.832HisLeu: 0.832 ± 0.26
0.312HisMet: 0.312 ± 0.203
0.832HisAsn: 0.832 ± 0.307
0.0HisPro: 0.0 ± 0.0
0.312HisGln: 0.312 ± 0.151
0.312HisArg: 0.312 ± 0.159
0.624HisSer: 0.624 ± 0.284
0.728HisThr: 0.728 ± 0.26
0.728HisVal: 0.728 ± 0.233
0.0HisTrp: 0.0 ± 0.0
0.936HisTyr: 0.936 ± 0.325
0.0HisXaa: 0.0 ± 0.0
Ile
4.263IleAla: 4.263 ± 0.617
1.144IleCys: 1.144 ± 0.512
6.551IleAsp: 6.551 ± 0.841
8.942IleGlu: 8.942 ± 0.97
2.808IlePhe: 2.808 ± 0.532
5.303IleGly: 5.303 ± 1.152
0.832IleHis: 0.832 ± 0.293
6.447IleIle: 6.447 ± 0.771
10.918IleLys: 10.918 ± 1.194
7.903IleLeu: 7.903 ± 0.914
1.976IleMet: 1.976 ± 0.501
5.511IleAsn: 5.511 ± 0.769
3.119IlePro: 3.119 ± 0.48
2.496IleGln: 2.496 ± 0.567
3.535IleArg: 3.535 ± 0.549
4.783IleSer: 4.783 ± 0.835
4.263IleThr: 4.263 ± 0.669
5.199IleVal: 5.199 ± 0.689
0.416IleTrp: 0.416 ± 0.282
4.055IleTyr: 4.055 ± 0.747
0.0IleXaa: 0.0 ± 0.0
Lys
7.175LysAla: 7.175 ± 0.802
1.144LysCys: 1.144 ± 0.354
6.655LysAsp: 6.655 ± 0.635
12.478LysGlu: 12.478 ± 1.157
3.639LysPhe: 3.639 ± 0.552
5.095LysGly: 5.095 ± 0.7
1.872LysHis: 1.872 ± 0.381
10.71LysIle: 10.71 ± 1.063
10.606LysLys: 10.606 ± 1.032
10.71LysLeu: 10.71 ± 1.306
2.704LysMet: 2.704 ± 0.601
5.927LysAsn: 5.927 ± 0.544
2.184LysPro: 2.184 ± 0.448
3.743LysGln: 3.743 ± 0.859
3.015LysArg: 3.015 ± 0.56
5.615LysSer: 5.615 ± 1.013
6.031LysThr: 6.031 ± 0.878
5.511LysVal: 5.511 ± 0.714
0.936LysTrp: 0.936 ± 0.258
4.367LysTyr: 4.367 ± 0.67
0.0LysXaa: 0.0 ± 0.0
Leu
5.095LeuAla: 5.095 ± 0.791
0.832LeuCys: 0.832 ± 0.338
6.759LeuAsp: 6.759 ± 0.827
7.903LeuGlu: 7.903 ± 1.313
3.327LeuPhe: 3.327 ± 0.553
3.535LeuGly: 3.535 ± 0.612
0.728LeuHis: 0.728 ± 0.293
6.239LeuIle: 6.239 ± 0.773
10.606LeuLys: 10.606 ± 0.947
6.239LeuLeu: 6.239 ± 0.811
1.976LeuMet: 1.976 ± 0.379
5.615LeuAsn: 5.615 ± 0.795
1.456LeuPro: 1.456 ± 0.397
2.288LeuGln: 2.288 ± 0.559
3.639LeuArg: 3.639 ± 0.677
6.135LeuSer: 6.135 ± 0.832
4.575LeuThr: 4.575 ± 0.881
3.223LeuVal: 3.223 ± 0.568
0.832LeuTrp: 0.832 ± 0.375
3.223LeuTyr: 3.223 ± 0.569
0.0LeuXaa: 0.0 ± 0.0
Met
1.768MetAla: 1.768 ± 0.687
0.0MetCys: 0.0 ± 0.0
1.144MetAsp: 1.144 ± 0.313
1.976MetGlu: 1.976 ± 0.478
1.248MetPhe: 1.248 ± 0.38
1.248MetGly: 1.248 ± 0.422
0.416MetHis: 0.416 ± 0.196
2.392MetIle: 2.392 ± 0.569
2.912MetLys: 2.912 ± 0.607
1.976MetLeu: 1.976 ± 0.396
0.208MetMet: 0.208 ± 0.153
2.08MetAsn: 2.08 ± 0.531
0.416MetPro: 0.416 ± 0.24
0.624MetGln: 0.624 ± 0.247
0.832MetArg: 0.832 ± 0.436
1.664MetSer: 1.664 ± 0.36
1.456MetThr: 1.456 ± 0.362
1.144MetVal: 1.144 ± 0.411
0.104MetTrp: 0.104 ± 0.097
0.936MetTyr: 0.936 ± 0.272
0.0MetXaa: 0.0 ± 0.0
Asn
3.639AsnAla: 3.639 ± 0.828
0.416AsnCys: 0.416 ± 0.188
2.912AsnAsp: 2.912 ± 0.643
5.615AsnGlu: 5.615 ± 0.8
2.496AsnPhe: 2.496 ± 0.387
3.951AsnGly: 3.951 ± 0.852
0.104AsnHis: 0.104 ± 0.096
7.695AsnIle: 7.695 ± 1.035
7.903AsnLys: 7.903 ± 0.865
4.887AsnLeu: 4.887 ± 0.757
1.56AsnMet: 1.56 ± 0.425
4.679AsnAsn: 4.679 ± 0.738
1.976AsnPro: 1.976 ± 0.569
0.832AsnGln: 0.832 ± 0.29
2.08AsnArg: 2.08 ± 0.453
4.679AsnSer: 4.679 ± 0.706
3.327AsnThr: 3.327 ± 0.532
4.159AsnVal: 4.159 ± 0.573
0.936AsnTrp: 0.936 ± 0.262
2.184AsnTyr: 2.184 ± 0.563
0.0AsnXaa: 0.0 ± 0.0
Pro
1.144ProAla: 1.144 ± 0.339
0.104ProCys: 0.104 ± 0.099
0.936ProAsp: 0.936 ± 0.307
1.144ProGlu: 1.144 ± 0.319
1.04ProPhe: 1.04 ± 0.334
1.04ProGly: 1.04 ± 0.284
0.52ProHis: 0.52 ± 0.219
1.872ProIle: 1.872 ± 0.418
1.768ProLys: 1.768 ± 0.392
1.56ProLeu: 1.56 ± 0.383
0.312ProMet: 0.312 ± 0.173
1.04ProAsn: 1.04 ± 0.379
0.208ProPro: 0.208 ± 0.145
0.312ProGln: 0.312 ± 0.173
0.832ProArg: 0.832 ± 0.352
2.496ProSer: 2.496 ± 0.561
1.872ProThr: 1.872 ± 0.379
1.56ProVal: 1.56 ± 0.651
0.312ProTrp: 0.312 ± 0.224
1.04ProTyr: 1.04 ± 0.348
0.0ProXaa: 0.0 ± 0.0
Gln
1.976GlnAla: 1.976 ± 0.552
0.104GlnCys: 0.104 ± 0.122
1.248GlnAsp: 1.248 ± 0.295
2.496GlnGlu: 2.496 ± 0.612
0.832GlnPhe: 0.832 ± 0.348
1.56GlnGly: 1.56 ± 0.434
0.104GlnHis: 0.104 ± 0.099
2.496GlnIle: 2.496 ± 0.508
2.08GlnLys: 2.08 ± 0.442
1.872GlnLeu: 1.872 ± 0.498
0.936GlnMet: 0.936 ± 0.416
1.352GlnAsn: 1.352 ± 0.396
0.416GlnPro: 0.416 ± 0.23
0.936GlnGln: 0.936 ± 0.404
0.832GlnArg: 0.832 ± 0.352
1.144GlnSer: 1.144 ± 0.326
1.456GlnThr: 1.456 ± 0.406
0.936GlnVal: 0.936 ± 0.314
0.104GlnTrp: 0.104 ± 0.096
1.56GlnTyr: 1.56 ± 0.279
0.0GlnXaa: 0.0 ± 0.0
Arg
1.248ArgAla: 1.248 ± 0.333
0.416ArgCys: 0.416 ± 0.184
1.248ArgAsp: 1.248 ± 0.29
3.327ArgGlu: 3.327 ± 0.592
0.936ArgPhe: 0.936 ± 0.261
2.184ArgGly: 2.184 ± 0.353
0.728ArgHis: 0.728 ± 0.296
3.431ArgIle: 3.431 ± 0.578
3.951ArgLys: 3.951 ± 0.665
1.872ArgLeu: 1.872 ± 0.457
0.936ArgMet: 0.936 ± 0.328
1.976ArgAsn: 1.976 ± 0.392
0.624ArgPro: 0.624 ± 0.257
1.04ArgGln: 1.04 ± 0.266
2.184ArgArg: 2.184 ± 0.56
1.768ArgSer: 1.768 ± 0.621
2.08ArgThr: 2.08 ± 0.452
1.664ArgVal: 1.664 ± 0.377
0.416ArgTrp: 0.416 ± 0.238
1.144ArgTyr: 1.144 ± 0.335
0.0ArgXaa: 0.0 ± 0.0
Ser
3.015SerAla: 3.015 ± 0.598
0.624SerCys: 0.624 ± 0.226
3.119SerAsp: 3.119 ± 0.579
4.887SerGlu: 4.887 ± 0.874
3.223SerPhe: 3.223 ± 0.526
3.119SerGly: 3.119 ± 0.689
0.312SerHis: 0.312 ± 0.163
4.159SerIle: 4.159 ± 0.713
7.071SerLys: 7.071 ± 0.787
5.199SerLeu: 5.199 ± 0.703
1.248SerMet: 1.248 ± 0.408
4.887SerAsn: 4.887 ± 0.843
1.352SerPro: 1.352 ± 0.419
0.936SerGln: 0.936 ± 0.318
1.768SerArg: 1.768 ± 0.335
3.535SerSer: 3.535 ± 0.723
3.223SerThr: 3.223 ± 0.581
2.496SerVal: 2.496 ± 0.56
0.832SerTrp: 0.832 ± 0.261
3.015SerTyr: 3.015 ± 0.525
0.0SerXaa: 0.0 ± 0.0
Thr
3.015ThrAla: 3.015 ± 0.745
0.52ThrCys: 0.52 ± 0.309
3.951ThrAsp: 3.951 ± 0.677
5.615ThrGlu: 5.615 ± 0.813
1.352ThrPhe: 1.352 ± 0.358
4.055ThrGly: 4.055 ± 0.896
0.208ThrHis: 0.208 ± 0.123
4.263ThrIle: 4.263 ± 0.594
6.031ThrLys: 6.031 ± 0.584
5.511ThrLeu: 5.511 ± 0.991
0.936ThrMet: 0.936 ± 0.355
3.535ThrAsn: 3.535 ± 0.591
1.248ThrPro: 1.248 ± 0.401
1.664ThrGln: 1.664 ± 0.57
1.248ThrArg: 1.248 ± 0.344
3.015ThrSer: 3.015 ± 0.533
4.263ThrThr: 4.263 ± 0.888
3.223ThrVal: 3.223 ± 0.499
0.624ThrTrp: 0.624 ± 0.273
2.08ThrTyr: 2.08 ± 0.342
0.0ThrXaa: 0.0 ± 0.0
Val
2.912ValAla: 2.912 ± 0.684
0.52ValCys: 0.52 ± 0.217
3.535ValAsp: 3.535 ± 0.54
3.535ValGlu: 3.535 ± 0.736
2.392ValPhe: 2.392 ± 0.417
3.015ValGly: 3.015 ± 0.733
0.416ValHis: 0.416 ± 0.173
3.847ValIle: 3.847 ± 0.666
6.343ValLys: 6.343 ± 0.958
4.471ValLeu: 4.471 ± 0.676
1.352ValMet: 1.352 ± 0.329
3.847ValAsn: 3.847 ± 0.706
1.352ValPro: 1.352 ± 0.299
1.872ValGln: 1.872 ± 0.421
1.352ValArg: 1.352 ± 0.454
3.535ValSer: 3.535 ± 0.638
3.223ValThr: 3.223 ± 0.742
4.367ValVal: 4.367 ± 0.754
0.416ValTrp: 0.416 ± 0.214
2.704ValTyr: 2.704 ± 0.499
0.0ValXaa: 0.0 ± 0.0
Trp
0.624TrpAla: 0.624 ± 0.265
0.416TrpCys: 0.416 ± 0.202
1.04TrpAsp: 1.04 ± 0.323
0.312TrpGlu: 0.312 ± 0.177
0.624TrpPhe: 0.624 ± 0.26
0.728TrpGly: 0.728 ± 0.238
0.208TrpHis: 0.208 ± 0.142
0.936TrpIle: 0.936 ± 0.302
0.936TrpLys: 0.936 ± 0.292
0.624TrpLeu: 0.624 ± 0.215
0.208TrpMet: 0.208 ± 0.154
0.624TrpAsn: 0.624 ± 0.216
0.0TrpPro: 0.0 ± 0.0
0.0TrpGln: 0.0 ± 0.0
0.104TrpArg: 0.104 ± 0.109
0.52TrpSer: 0.52 ± 0.235
0.52TrpThr: 0.52 ± 0.247
0.728TrpVal: 0.728 ± 0.264
0.208TrpTrp: 0.208 ± 0.168
0.208TrpTyr: 0.208 ± 0.15
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.664TyrAla: 1.664 ± 0.374
0.52TyrCys: 0.52 ± 0.254
3.431TyrAsp: 3.431 ± 0.658
3.431TyrGlu: 3.431 ± 0.618
1.768TyrPhe: 1.768 ± 0.48
1.248TyrGly: 1.248 ± 0.439
0.52TyrHis: 0.52 ± 0.198
4.159TyrIle: 4.159 ± 0.488
4.263TyrLys: 4.263 ± 0.613
2.288TyrLeu: 2.288 ± 0.523
0.728TyrMet: 0.728 ± 0.273
3.431TyrAsn: 3.431 ± 0.707
1.248TyrPro: 1.248 ± 0.333
0.728TyrGln: 0.728 ± 0.277
1.664TyrArg: 1.664 ± 0.535
2.808TyrSer: 2.808 ± 0.608
3.327TyrThr: 3.327 ± 0.615
2.08TyrVal: 2.08 ± 0.419
0.416TyrTrp: 0.416 ± 0.191
1.456TyrTyr: 1.456 ± 0.371
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 50 proteins (9618 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski