Amino acid dipepetide frequency for Microbacterium laevaniformans

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	23.368AlaAla: 23.368 ± 0.235	0.752AlaCys: 0.752 ± 0.031	8.876AlaAsp: 8.876 ± 0.124	7.776AlaGlu: 7.776 ± 0.111	4.0AlaPhe: 4.0 ± 0.064	12.467AlaGly: 12.467 ± 0.147	2.857AlaHis: 2.857 ± 0.061	5.922AlaIle: 5.922 ± 0.084	2.565AlaLys: 2.565 ± 0.07	14.94AlaLeu: 14.94 ± 0.16	2.781AlaMet: 2.781 ± 0.057	2.159AlaAsn: 2.159 ± 0.058	7.15AlaPro: 7.15 ± 0.107	4.173AlaGln: 4.173 ± 0.073	10.047AlaArg: 10.047 ± 0.127	7.666AlaSer: 7.666 ± 0.103	7.893AlaThr: 7.893 ± 0.1	12.514AlaVal: 12.514 ± 0.143	1.999AlaTrp: 1.999 ± 0.052	2.475AlaTyr: 2.475 ± 0.05	0.0AlaXaa: 0.0 ± 0.0
Cys	0.75CysAla: 0.75 ± 0.03	0.056CysCys: 0.056 ± 0.008	0.334CysAsp: 0.334 ± 0.02	0.236CysGlu: 0.236 ± 0.018	0.144CysPhe: 0.144 ± 0.013	0.585CysGly: 0.585 ± 0.027	0.12CysHis: 0.12 ± 0.012	0.158CysIle: 0.158 ± 0.014	0.061CysLys: 0.061 ± 0.008	0.438CysLeu: 0.438 ± 0.023	0.084CysMet: 0.084 ± 0.013	0.098CysAsn: 0.098 ± 0.011	0.311CysPro: 0.311 ± 0.02	0.109CysGln: 0.109 ± 0.012	0.399CysArg: 0.399 ± 0.023	0.303CysSer: 0.303 ± 0.021	0.345CysThr: 0.345 ± 0.023	0.393CysVal: 0.393 ± 0.022	0.061CysTrp: 0.061 ± 0.01	0.106CysTyr: 0.106 ± 0.014	0.0CysXaa: 0.0 ± 0.0
Asp	10.319AspAla: 10.319 ± 0.122	0.236AspCys: 0.236 ± 0.018	4.398AspAsp: 4.398 ± 0.083	4.216AspGlu: 4.216 ± 0.075	1.566AspPhe: 1.566 ± 0.047	6.27AspGly: 6.27 ± 0.096	1.163AspHis: 1.163 ± 0.034	2.442AspIle: 2.442 ± 0.058	1.04AspLys: 1.04 ± 0.041	6.167AspLeu: 6.167 ± 0.087	0.838AspMet: 0.838 ± 0.032	0.934AspAsn: 0.934 ± 0.032	4.136AspPro: 4.136 ± 0.074	1.563AspGln: 1.563 ± 0.043	4.584AspArg: 4.584 ± 0.076	2.287AspSer: 2.287 ± 0.05	3.002AspThr: 3.002 ± 0.06	5.486AspVal: 5.486 ± 0.083	0.862AspTrp: 0.862 ± 0.031	1.174AspTyr: 1.174 ± 0.038	0.0AspXaa: 0.0 ± 0.0
Glu	6.551GluAla: 6.551 ± 0.098	0.223GluCys: 0.223 ± 0.016	2.55GluAsp: 2.55 ± 0.064	2.698GluGlu: 2.698 ± 0.062	1.55GluPhe: 1.55 ± 0.044	3.668GluGly: 3.668 ± 0.079	1.34GluHis: 1.34 ± 0.042	2.773GluIle: 2.773 ± 0.051	1.37GluLys: 1.37 ± 0.045	6.137GluLeu: 6.137 ± 0.085	0.838GluMet: 0.838 ± 0.034	1.164GluAsn: 1.164 ± 0.036	2.615GluPro: 2.615 ± 0.056	1.955GluGln: 1.955 ± 0.042	5.089GluArg: 5.089 ± 0.096	2.526GluSer: 2.526 ± 0.053	2.864GluThr: 2.864 ± 0.059	4.418GluVal: 4.418 ± 0.078	0.75GluTrp: 0.75 ± 0.03	1.023GluTyr: 1.023 ± 0.038	0.0GluXaa: 0.0 ± 0.0
Phe	4.461PheAla: 4.461 ± 0.08	0.146PheCys: 0.146 ± 0.015	2.296PheAsp: 2.296 ± 0.056	1.565PheGlu: 1.565 ± 0.048	1.065PhePhe: 1.065 ± 0.034	3.298PheGly: 3.298 ± 0.074	0.548PheHis: 0.548 ± 0.024	1.125PheIle: 1.125 ± 0.039	0.394PheLys: 0.394 ± 0.026	2.712PheLeu: 2.712 ± 0.059	0.413PheMet: 0.413 ± 0.022	0.611PheAsn: 0.611 ± 0.027	1.38PhePro: 1.38 ± 0.038	0.737PheGln: 0.737 ± 0.033	1.775PheArg: 1.775 ± 0.043	1.726PheSer: 1.726 ± 0.048	2.067PheThr: 2.067 ± 0.051	2.862PheVal: 2.862 ± 0.061	0.445PheTrp: 0.445 ± 0.024	0.617PheTyr: 0.617 ± 0.027	0.0PheXaa: 0.0 ± 0.0
Gly	11.367GlyAla: 11.367 ± 0.145	0.59GlyCys: 0.59 ± 0.034	5.106GlyAsp: 5.106 ± 0.081	4.666GlyGlu: 4.666 ± 0.075	3.15GlyPhe: 3.15 ± 0.068	7.579GlyGly: 7.579 ± 0.113	1.807GlyHis: 1.807 ± 0.049	4.912GlyIle: 4.912 ± 0.091	2.071GlyLys: 2.071 ± 0.06	8.387GlyLeu: 8.387 ± 0.105	1.969GlyMet: 1.969 ± 0.045	1.559GlyAsn: 1.559 ± 0.048	3.649GlyPro: 3.649 ± 0.062	2.513GlyGln: 2.513 ± 0.056	6.489GlyArg: 6.489 ± 0.1	5.014GlySer: 5.014 ± 0.084	5.695GlyThr: 5.695 ± 0.083	8.03GlyVal: 8.03 ± 0.117	1.69GlyTrp: 1.69 ± 0.046	2.163GlyTyr: 2.163 ± 0.054	0.0GlyXaa: 0.0 ± 0.0
His	2.632HisAla: 2.632 ± 0.055	0.104HisCys: 0.104 ± 0.011	1.341HisAsp: 1.341 ± 0.04	1.084HisGlu: 1.084 ± 0.04	0.471HisPhe: 0.471 ± 0.028	1.946HisGly: 1.946 ± 0.051	0.583HisHis: 0.583 ± 0.028	0.707HisIle: 0.707 ± 0.029	0.278HisLys: 0.278 ± 0.018	2.06HisLeu: 2.06 ± 0.049	0.31HisMet: 0.31 ± 0.019	0.337HisAsn: 0.337 ± 0.02	1.52HisPro: 1.52 ± 0.043	0.484HisGln: 0.484 ± 0.022	1.621HisArg: 1.621 ± 0.05	0.922HisSer: 0.922 ± 0.027	1.06HisThr: 1.06 ± 0.04	1.786HisVal: 1.786 ± 0.046	0.254HisTrp: 0.254 ± 0.017	0.42HisTyr: 0.42 ± 0.025	0.0HisXaa: 0.0 ± 0.0
Ile	7.287IleAla: 7.287 ± 0.091	0.224IleCys: 0.224 ± 0.018	3.622IleAsp: 3.622 ± 0.071	2.645IleGlu: 2.645 ± 0.05	1.089IlePhe: 1.089 ± 0.043	4.508IleGly: 4.508 ± 0.09	0.751IleHis: 0.751 ± 0.028	1.777IleIle: 1.777 ± 0.05	0.727IleLys: 0.727 ± 0.036	3.683IleLeu: 3.683 ± 0.066	0.626IleMet: 0.626 ± 0.026	0.835IleAsn: 0.835 ± 0.034	2.39IlePro: 2.39 ± 0.045	0.981IleGln: 0.981 ± 0.036	2.766IleArg: 2.766 ± 0.059	2.311IleSer: 2.311 ± 0.058	2.815IleThr: 2.815 ± 0.061	4.906IleVal: 4.906 ± 0.077	0.512IleTrp: 0.512 ± 0.025	0.731IleTyr: 0.731 ± 0.032	0.0IleXaa: 0.0 ± 0.0
Lys	2.384LysAla: 2.384 ± 0.072	0.061LysCys: 0.061 ± 0.008	1.106LysAsp: 1.106 ± 0.037	0.823LysGlu: 0.823 ± 0.038	0.399LysPhe: 0.399 ± 0.023	1.549LysGly: 1.549 ± 0.055	0.379LysHis: 0.379 ± 0.019	0.949LysIle: 0.949 ± 0.033	0.798LysLys: 0.798 ± 0.041	1.837LysLeu: 1.837 ± 0.052	0.363LysMet: 0.363 ± 0.021	0.54LysAsn: 0.54 ± 0.027	1.084LysPro: 1.084 ± 0.037	0.653LysGln: 0.653 ± 0.029	1.402LysArg: 1.402 ± 0.048	0.997LysSer: 0.997 ± 0.038	1.352LysThr: 1.352 ± 0.048	1.612LysVal: 1.612 ± 0.053	0.224LysTrp: 0.224 ± 0.016	0.422LysTyr: 0.422 ± 0.022	0.0LysXaa: 0.0 ± 0.0
Leu	14.667LeuAla: 14.667 ± 0.158	0.526LeuCys: 0.526 ± 0.024	6.445LeuAsp: 6.445 ± 0.088	4.894LeuGlu: 4.894 ± 0.075	2.832LeuPhe: 2.832 ± 0.067	8.826LeuGly: 8.826 ± 0.12	1.781LeuHis: 1.781 ± 0.051	4.5LeuIle: 4.5 ± 0.081	1.68LeuLys: 1.68 ± 0.054	9.997LeuLeu: 9.997 ± 0.133	1.591LeuMet: 1.591 ± 0.043	1.603LeuAsn: 1.603 ± 0.04	5.507LeuPro: 5.507 ± 0.079	2.597LeuGln: 2.597 ± 0.05	7.884LeuArg: 7.884 ± 0.105	5.911LeuSer: 5.911 ± 0.096	6.551LeuThr: 6.551 ± 0.085	9.486LeuVal: 9.486 ± 0.124	1.387LeuTrp: 1.387 ± 0.045	1.614LeuTyr: 1.614 ± 0.044	0.0LeuXaa: 0.0 ± 0.0
Met	2.119MetAla: 2.119 ± 0.047	0.1MetCys: 0.1 ± 0.012	0.825MetAsp: 0.825 ± 0.036	0.594MetGlu: 0.594 ± 0.027	0.53MetPhe: 0.53 ± 0.023	1.293MetGly: 1.293 ± 0.042	0.313MetHis: 0.313 ± 0.021	0.89MetIle: 0.89 ± 0.036	0.4MetLys: 0.4 ± 0.022	1.885MetLeu: 1.885 ± 0.047	0.364MetMet: 0.364 ± 0.022	0.466MetAsn: 0.466 ± 0.024	1.189MetPro: 1.189 ± 0.038	0.506MetGln: 0.506 ± 0.022	1.35MetArg: 1.35 ± 0.045	1.536MetSer: 1.536 ± 0.043	1.696MetThr: 1.696 ± 0.047	1.316MetVal: 1.316 ± 0.039	0.227MetTrp: 0.227 ± 0.015	0.255MetTyr: 0.255 ± 0.017	0.0MetXaa: 0.0 ± 0.0
Asn	2.447AsnAla: 2.447 ± 0.06	0.105AsnCys: 0.105 ± 0.011	1.099AsnAsp: 1.099 ± 0.04	0.873AsnGlu: 0.873 ± 0.032	0.549AsnPhe: 0.549 ± 0.032	1.759AsnGly: 1.759 ± 0.053	0.323AsnHis: 0.323 ± 0.017	0.794AsnIle: 0.794 ± 0.036	0.404AsnLys: 0.404 ± 0.021	1.848AsnLeu: 1.848 ± 0.041	0.32AsnMet: 0.32 ± 0.023	0.427AsnAsn: 0.427 ± 0.023	1.455AsnPro: 1.455 ± 0.04	0.508AsnGln: 0.508 ± 0.026	1.152AsnArg: 1.152 ± 0.039	0.85AsnSer: 0.85 ± 0.028	1.104AsnThr: 1.104 ± 0.034	1.562AsnVal: 1.562 ± 0.046	0.273AsnTrp: 0.273 ± 0.017	0.422AsnTyr: 0.422 ± 0.023	0.0AsnXaa: 0.0 ± 0.0
Pro	7.716ProAla: 7.716 ± 0.113	0.194ProCys: 0.194 ± 0.014	3.817ProAsp: 3.817 ± 0.073	3.285ProGlu: 3.285 ± 0.057	1.729ProPhe: 1.729 ± 0.043	4.797ProGly: 4.797 ± 0.085	1.147ProHis: 1.147 ± 0.033	2.101ProIle: 2.101 ± 0.049	0.925ProLys: 0.925 ± 0.037	5.056ProLeu: 5.056 ± 0.08	0.867ProMet: 0.867 ± 0.031	0.95ProAsn: 0.95 ± 0.034	2.385ProPro: 2.385 ± 0.061	1.584ProGln: 1.584 ± 0.036	3.776ProArg: 3.776 ± 0.074	3.115ProSer: 3.115 ± 0.063	3.619ProThr: 3.619 ± 0.079	4.938ProVal: 4.938 ± 0.084	0.891ProTrp: 0.891 ± 0.034	1.03ProTyr: 1.03 ± 0.037	0.0ProXaa: 0.0 ± 0.0
Gln	3.417GlnAla: 3.417 ± 0.071	0.126GlnCys: 0.126 ± 0.012	1.235GlnAsp: 1.235 ± 0.039	1.211GlnGlu: 1.211 ± 0.04	0.819GlnPhe: 0.819 ± 0.033	2.003GlnGly: 2.003 ± 0.05	0.596GlnHis: 0.596 ± 0.023	1.507GlnIle: 1.507 ± 0.044	0.66GlnLys: 0.66 ± 0.03	3.171GlnLeu: 3.171 ± 0.062	0.506GlnMet: 0.506 ± 0.023	0.646GlnAsn: 0.646 ± 0.031	1.576GlnPro: 1.576 ± 0.042	1.24GlnGln: 1.24 ± 0.041	2.519GlnArg: 2.519 ± 0.052	1.551GlnSer: 1.551 ± 0.05	1.712GlnThr: 1.712 ± 0.044	2.318GlnVal: 2.318 ± 0.047	0.516GlnTrp: 0.516 ± 0.025	0.567GlnTyr: 0.567 ± 0.03	0.0GlnXaa: 0.0 ± 0.0
Arg	9.939ArgAla: 9.939 ± 0.136	0.327ArgCys: 0.327 ± 0.025	4.527ArgAsp: 4.527 ± 0.069	4.276ArgGlu: 4.276 ± 0.081	2.43ArgPhe: 2.43 ± 0.053	5.787ArgGly: 5.787 ± 0.077	1.644ArgHis: 1.644 ± 0.041	3.546ArgIle: 3.546 ± 0.059	1.282ArgLys: 1.282 ± 0.044	7.587ArgLeu: 7.587 ± 0.113	1.751ArgMet: 1.751 ± 0.045	1.287ArgAsn: 1.287 ± 0.038	3.726ArgPro: 3.726 ± 0.071	2.039ArgGln: 2.039 ± 0.044	7.244ArgArg: 7.244 ± 0.125	3.946ArgSer: 3.946 ± 0.071	4.459ArgThr: 4.459 ± 0.079	6.588ArgVal: 6.588 ± 0.105	1.273ArgTrp: 1.273 ± 0.042	1.59ArgTyr: 1.59 ± 0.045	0.0ArgXaa: 0.0 ± 0.0
Ser	7.676SerAla: 7.676 ± 0.104	0.266SerCys: 0.266 ± 0.018	3.216SerAsp: 3.216 ± 0.062	2.378SerGlu: 2.378 ± 0.053	1.849SerPhe: 1.849 ± 0.052	5.581SerGly: 5.581 ± 0.084	1.001SerHis: 1.001 ± 0.033	2.398SerIle: 2.398 ± 0.05	0.914SerLys: 0.914 ± 0.036	5.135SerLeu: 5.135 ± 0.077	1.167SerMet: 1.167 ± 0.038	0.949SerAsn: 0.949 ± 0.035	3.149SerPro: 3.149 ± 0.066	1.38SerGln: 1.38 ± 0.042	3.737SerArg: 3.737 ± 0.071	3.437SerSer: 3.437 ± 0.066	3.665SerThr: 3.665 ± 0.067	4.522SerVal: 4.522 ± 0.068	0.944SerTrp: 0.944 ± 0.032	1.159SerTyr: 1.159 ± 0.038	0.0SerXaa: 0.0 ± 0.0
Thr	8.515ThrAla: 8.515 ± 0.122	0.294ThrCys: 0.294 ± 0.018	3.68ThrAsp: 3.68 ± 0.065	2.649ThrGlu: 2.649 ± 0.057	1.857ThrPhe: 1.857 ± 0.044	5.955ThrGly: 5.955 ± 0.091	1.196ThrHis: 1.196 ± 0.041	2.933ThrIle: 2.933 ± 0.058	1.164ThrLys: 1.164 ± 0.042	6.155ThrLeu: 6.155 ± 0.097	0.96ThrMet: 0.96 ± 0.036	1.138ThrAsn: 1.138 ± 0.038	4.27ThrPro: 4.27 ± 0.079	1.525ThrGln: 1.525 ± 0.043	4.02ThrArg: 4.02 ± 0.068	3.451ThrSer: 3.451 ± 0.062	4.05ThrThr: 4.05 ± 0.079	6.021ThrVal: 6.021 ± 0.1	0.892ThrTrp: 0.892 ± 0.038	1.164ThrTyr: 1.164 ± 0.04	0.0ThrXaa: 0.0 ± 0.0
Val	12.571ValAla: 12.571 ± 0.139	0.548ValCys: 0.548 ± 0.027	6.139ValAsp: 6.139 ± 0.101	4.589ValGlu: 4.589 ± 0.078	2.978ValPhe: 2.978 ± 0.068	7.565ValGly: 7.565 ± 0.104	1.732ValHis: 1.732 ± 0.051	4.399ValIle: 4.399 ± 0.084	1.555ValLys: 1.555 ± 0.048	9.225ValLeu: 9.225 ± 0.125	1.473ValMet: 1.473 ± 0.042	1.691ValAsn: 1.691 ± 0.047	4.708ValPro: 4.708 ± 0.073	2.208ValGln: 2.208 ± 0.049	6.425ValArg: 6.425 ± 0.099	5.115ValSer: 5.115 ± 0.078	5.849ValThr: 5.849 ± 0.091	9.209ValVal: 9.209 ± 0.116	1.255ValTrp: 1.255 ± 0.041	1.593ValTyr: 1.593 ± 0.043	0.0ValXaa: 0.0 ± 0.0
Trp	1.705TrpAla: 1.705 ± 0.045	0.104TrpCys: 0.104 ± 0.01	0.807TrpAsp: 0.807 ± 0.03	0.599TrpGlu: 0.599 ± 0.032	0.568TrpPhe: 0.568 ± 0.027	1.093TrpGly: 1.093 ± 0.039	0.342TrpHis: 0.342 ± 0.02	0.694TrpIle: 0.694 ± 0.028	0.298TrpLys: 0.298 ± 0.018	1.771TrpLeu: 1.771 ± 0.048	0.375TrpMet: 0.375 ± 0.018	0.416TrpAsn: 0.416 ± 0.02	0.706TrpPro: 0.706 ± 0.031	0.581TrpGln: 0.581 ± 0.026	1.311TrpArg: 1.311 ± 0.043	0.926TrpSer: 0.926 ± 0.031	0.968TrpThr: 0.968 ± 0.036	1.246TrpVal: 1.246 ± 0.032	0.378TrpTrp: 0.378 ± 0.026	0.273TrpTyr: 0.273 ± 0.018	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.49TyrAla: 2.49 ± 0.057	0.11TyrCys: 0.11 ± 0.01	1.266TyrAsp: 1.266 ± 0.04	1.047TyrGlu: 1.047 ± 0.037	0.666TyrPhe: 0.666 ± 0.03	1.877TyrGly: 1.877 ± 0.046	0.294TyrHis: 0.294 ± 0.02	0.66TyrIle: 0.66 ± 0.029	0.348TyrLys: 0.348 ± 0.022	2.092TyrLeu: 2.092 ± 0.047	0.259TyrMet: 0.259 ± 0.015	0.463TyrAsn: 0.463 ± 0.023	1.015TyrPro: 1.015 ± 0.032	0.56TyrGln: 0.56 ± 0.025	1.656TyrArg: 1.656 ± 0.042	0.933TyrSer: 0.933 ± 0.03	1.125TyrThr: 1.125 ± 0.039	1.613TyrVal: 1.613 ± 0.043	0.325TyrTrp: 0.325 ± 0.02	0.381TyrTyr: 0.381 ± 0.024	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 2869 proteins (906975 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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