Beet cryptic virus 1

Taxonomy: Viruses; Riboviria; Orthornavirae; Pisuviricota; Duplopiviricetes; Durnavirales; Partitiviridae; Alphapartitivirus

Average proteome isoelectric point is 6.31

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|B2CGP4|B2CGP4_9VIRU Coat protein OS=Beet cryptic virus 1 OX=509923 GN=CP PE=4 SV=1
MM1 pKa = 7.65EE2 pKa = 5.7NNTPLANPSGPNVPSAAAPPTPAPPAIPQATPTPGAVSQPPAPPARR48 pKa = 11.84RR49 pKa = 11.84SRR51 pKa = 11.84TPRR54 pKa = 11.84GPVPQATPGSTSGAPALLEE73 pKa = 4.05LSAGLPMYY81 pKa = 7.42TVPRR85 pKa = 11.84RR86 pKa = 11.84GVNTFVPDD94 pKa = 3.46AQMLFHH100 pKa = 6.86VLGICDD106 pKa = 3.86QMMLSTDD113 pKa = 3.09RR114 pKa = 11.84FTRR117 pKa = 11.84SSPAWIPIVSQLYY130 pKa = 10.22ISVLWMVAILRR141 pKa = 11.84VFVASGYY148 pKa = 9.09GALYY152 pKa = 10.6SSLINDD158 pKa = 5.42LIGHH162 pKa = 6.3LRR164 pKa = 11.84IDD166 pKa = 3.56EE167 pKa = 4.15CMIPGPLVPFFQSLGAVCGPYY188 pKa = 10.28EE189 pKa = 4.28WIGDD193 pKa = 3.7IVAAFPDD200 pKa = 5.36FLTLWDD206 pKa = 4.75AEE208 pKa = 4.59NFCPTADD215 pKa = 3.98LARR218 pKa = 11.84TCPVPAIMLDD228 pKa = 3.39QLHH231 pKa = 6.39YY232 pKa = 10.03FATWTIPAEE241 pKa = 4.1QILYY245 pKa = 10.04TNFQWYY251 pKa = 10.28RR252 pKa = 11.84NIFSLGLGAGNANNRR267 pKa = 11.84IGPQLCGSLYY277 pKa = 10.52SPRR280 pKa = 11.84AQVDD284 pKa = 3.47SARR287 pKa = 11.84AFWNAALSSGITRR300 pKa = 11.84TNAAEE305 pKa = 4.07ANGAFYY311 pKa = 9.67TYY313 pKa = 10.96AQLLGFISQNGTLQLDD329 pKa = 4.28WFQQVAVVMQKK340 pKa = 8.58YY341 pKa = 6.0TQYY344 pKa = 11.03FNGSTPLKK352 pKa = 9.96SISTIGIGAVAVIGAPTPDD371 pKa = 3.91PATRR375 pKa = 11.84DD376 pKa = 3.13WFYY379 pKa = 10.7PAATGIEE386 pKa = 4.16PFLCSRR392 pKa = 11.84FAPRR396 pKa = 11.84RR397 pKa = 11.84EE398 pKa = 3.99IPNTLGMIFSHH409 pKa = 7.11ADD411 pKa = 3.1HH412 pKa = 6.65EE413 pKa = 4.56LEE415 pKa = 4.25EE416 pKa = 4.19QAEE419 pKa = 4.33QYY421 pKa = 11.25AILTHH426 pKa = 6.0TNIRR430 pKa = 11.84WSPSVVAQNAWTAVNDD446 pKa = 4.24GASRR450 pKa = 11.84NGDD453 pKa = 3.25YY454 pKa = 10.44WIMMNYY460 pKa = 9.8RR461 pKa = 11.84FSTRR465 pKa = 11.84ISLKK469 pKa = 7.94TQFAQVIASRR479 pKa = 11.84YY480 pKa = 7.5HH481 pKa = 4.12QQAANRR487 pKa = 11.84VDD489 pKa = 3.2

Molecular weight: 53.36 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|B2CGP4|B2CGP4_9VIRU Coat protein OS=Beet cryptic virus 1 OX=509923 GN=CP PE=4 SV=1
MM1 pKa = 8.32DD2 pKa = 4.54YY3 pKa = 9.87LTSAFNRR10 pKa = 11.84ITHH13 pKa = 6.46WFNVPSNLEE22 pKa = 4.12YY23 pKa = 10.43IGTFSLPPGILRR35 pKa = 11.84VNEE38 pKa = 3.98VAISNHH44 pKa = 5.66KK45 pKa = 8.79KK46 pKa = 8.16TLEE49 pKa = 3.89HH50 pKa = 6.7SFNKK54 pKa = 9.98YY55 pKa = 10.23LYY57 pKa = 8.7AHH59 pKa = 7.41EE60 pKa = 5.06IKK62 pKa = 10.74LITQDD67 pKa = 3.55YY68 pKa = 9.07RR69 pKa = 11.84RR70 pKa = 11.84SDD72 pKa = 3.13IDD74 pKa = 3.64EE75 pKa = 4.23EE76 pKa = 4.91SILADD81 pKa = 4.29FFSGDD86 pKa = 3.36VEE88 pKa = 4.33KK89 pKa = 11.31FEE91 pKa = 4.84VPFDD95 pKa = 3.62EE96 pKa = 5.13HH97 pKa = 8.54VEE99 pKa = 4.09TGLRR103 pKa = 11.84CMADD107 pKa = 2.92AFRR110 pKa = 11.84PPRR113 pKa = 11.84LCRR116 pKa = 11.84PAHH119 pKa = 6.03ILDD122 pKa = 3.78VKK124 pKa = 10.61HH125 pKa = 6.44GYY127 pKa = 8.11PYY129 pKa = 10.43KK130 pKa = 10.06WNVNAEE136 pKa = 4.36PPFSTDD142 pKa = 3.36EE143 pKa = 4.02YY144 pKa = 11.11FLNQRR149 pKa = 11.84KK150 pKa = 7.03TFGEE154 pKa = 4.96FIRR157 pKa = 11.84MHH159 pKa = 6.57EE160 pKa = 4.18YY161 pKa = 10.71EE162 pKa = 5.8HH163 pKa = 7.06IDD165 pKa = 3.09KK166 pKa = 11.16ADD168 pKa = 3.68FFRR171 pKa = 11.84RR172 pKa = 11.84HH173 pKa = 6.43PNTEE177 pKa = 3.6SHH179 pKa = 6.91DD180 pKa = 4.58LIRR183 pKa = 11.84TIVPPKK189 pKa = 10.24FGYY192 pKa = 9.49LKK194 pKa = 11.12SMIFSWTRR202 pKa = 11.84RR203 pKa = 11.84WHH205 pKa = 6.41HH206 pKa = 6.48IIKK209 pKa = 10.51EE210 pKa = 4.24GFTEE214 pKa = 4.22STGLHH219 pKa = 4.48TTGYY223 pKa = 9.46FYY225 pKa = 11.38NRR227 pKa = 11.84FIFPMLLHH235 pKa = 6.66TKK237 pKa = 8.28TAIVKK242 pKa = 10.05QDD244 pKa = 4.31DD245 pKa = 4.02PNKK248 pKa = 9.92MRR250 pKa = 11.84TIWGASKK257 pKa = 9.78PWIIAEE263 pKa = 4.12TMLYY267 pKa = 9.2WEE269 pKa = 4.49YY270 pKa = 10.8IAWIKK275 pKa = 10.47LNPSVTPMLWGYY287 pKa = 7.52EE288 pKa = 4.17TFTGGWFRR296 pKa = 11.84LNRR299 pKa = 11.84DD300 pKa = 4.2LFCGFLQRR308 pKa = 11.84SFLTLDD314 pKa = 3.43WSRR317 pKa = 11.84FDD319 pKa = 3.15KK320 pKa = 10.78RR321 pKa = 11.84AYY323 pKa = 10.14FPLLRR328 pKa = 11.84RR329 pKa = 11.84ILYY332 pKa = 6.53TARR335 pKa = 11.84TFLTFDD341 pKa = 2.91EE342 pKa = 6.12GYY344 pKa = 10.56VPTHH348 pKa = 6.37SAPTHH353 pKa = 5.49PQWDD357 pKa = 3.61HH358 pKa = 5.16TKK360 pKa = 10.6AIRR363 pKa = 11.84LEE365 pKa = 4.25RR366 pKa = 11.84LWLWTLEE373 pKa = 4.18NLFEE377 pKa = 4.94APIILPDD384 pKa = 2.89GRR386 pKa = 11.84MYY388 pKa = 10.65RR389 pKa = 11.84RR390 pKa = 11.84HH391 pKa = 5.78FAGIPSGLFITQLLDD406 pKa = 2.7SWYY409 pKa = 9.85NYY411 pKa = 9.33TMLATLLSALGFDD424 pKa = 4.53PKK426 pKa = 10.86HH427 pKa = 6.68CIIKK431 pKa = 10.05VQGDD435 pKa = 3.54DD436 pKa = 3.82SIIRR440 pKa = 11.84LNVLVPQDD448 pKa = 3.3QHH450 pKa = 7.33QNLMDD455 pKa = 4.24NLVQLAVNYY464 pKa = 9.3FNAVVNVKK472 pKa = 10.2KK473 pKa = 10.96SEE475 pKa = 4.14FGNSLNGRR483 pKa = 11.84EE484 pKa = 3.96VLSYY488 pKa = 10.61RR489 pKa = 11.84NHH491 pKa = 6.46NGFPHH496 pKa = 7.07RR497 pKa = 11.84DD498 pKa = 3.77EE499 pKa = 5.66IMMLAQFYY507 pKa = 7.88HH508 pKa = 6.11TKK510 pKa = 10.61ARR512 pKa = 11.84DD513 pKa = 3.58PTPEE517 pKa = 3.33ITMAQAIGFAYY528 pKa = 10.22ASCANNKK535 pKa = 9.15RR536 pKa = 11.84VLWALKK542 pKa = 10.2DD543 pKa = 3.49VYY545 pKa = 10.88DD546 pKa = 3.87YY547 pKa = 11.81YY548 pKa = 11.61KK549 pKa = 11.07DD550 pKa = 3.95LGYY553 pKa = 9.29TPNRR557 pKa = 11.84AGLTLTFGDD566 pKa = 4.33SPDD569 pKa = 3.58LFVPEE574 pKa = 4.48ISLEE578 pKa = 4.07HH579 pKa = 6.61FPTEE583 pKa = 4.19TEE585 pKa = 3.13IRR587 pKa = 11.84RR588 pKa = 11.84YY589 pKa = 8.3LTSTSYY595 pKa = 11.44LNEE598 pKa = 4.1AQNARR603 pKa = 11.84TWPRR607 pKa = 11.84TLFINAPAQQ616 pKa = 3.31

Molecular weight: 72.49 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

1105

489

616

552.5

62.93

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.597 ± 2.231

1.086 ± 0.222

4.706 ± 0.659

4.253 ± 1.026

5.973 ± 0.817

5.43 ± 0.717

2.896 ± 0.942

6.335 ± 0.003

2.715 ± 1.352

8.869 ± 0.706

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.353 ± 0.065

5.068 ± 0.103

7.421 ± 1.278

3.71 ± 1.033

6.063 ± 0.48

5.792 ± 0.747

7.059 ± 0.2

4.615 ± 0.583

2.624 ± 0.11

4.434 ± 0.485
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski