Streptococcus satellite phage Javan250

Taxonomy: Viruses; unclassified bacterial viruses

Average proteome isoelectric point is 6.24

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 25 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D5ZJ99|A0A4D5ZJ99_9VIRU Capsid protein OS=Streptococcus satellite phage Javan250 OX=2558591 GN=JavanS250_0004 PE=4 SV=1
MM1 pKa = 6.89TVEE4 pKa = 4.93LIDD7 pKa = 3.61YY8 pKa = 9.06KK9 pKa = 11.18LWFEE13 pKa = 5.39DD14 pKa = 3.56IEE16 pKa = 4.48STARR20 pKa = 11.84KK21 pKa = 8.91FNCSVPEE28 pKa = 3.8INNYY32 pKa = 10.25LIEE35 pKa = 4.44KK36 pKa = 10.23LGVTPYY42 pKa = 11.49DD43 pKa = 5.09DD44 pKa = 5.18IYY46 pKa = 11.73NLNNNN51 pKa = 3.9

Molecular weight: 6.06 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D5ZH60|A0A4D5ZH60_9VIRU Putative transcriptional regulator OS=Streptococcus satellite phage Javan250 OX=2558591 GN=JavanS250_0020 PE=4 SV=1
MM1 pKa = 7.65TDD3 pKa = 3.12LEE5 pKa = 4.34KK6 pKa = 10.92RR7 pKa = 11.84LLRR10 pKa = 11.84LLKK13 pKa = 10.42VGKK16 pKa = 10.22EE17 pKa = 4.0NATPTAEE24 pKa = 4.64LAGLLNIDD32 pKa = 3.0IRR34 pKa = 11.84TIRR37 pKa = 11.84NLVRR41 pKa = 11.84SLRR44 pKa = 11.84LKK46 pKa = 10.65HH47 pKa = 5.49GVAIIGEE54 pKa = 4.44RR55 pKa = 11.84GTSAKK60 pKa = 10.41GLYY63 pKa = 9.67IPANDD68 pKa = 3.7TEE70 pKa = 4.29RR71 pKa = 11.84LEE73 pKa = 4.5GVRR76 pKa = 11.84ALDD79 pKa = 3.39NQVNEE84 pKa = 3.99EE85 pKa = 4.29RR86 pKa = 11.84KK87 pKa = 8.65TITALLNADD96 pKa = 3.1LRR98 pKa = 11.84EE99 pKa = 3.98FRR101 pKa = 11.84RR102 pKa = 11.84VLDD105 pKa = 3.67GRR107 pKa = 4.07

Molecular weight: 12.03 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

25

0

25

3763

50

535

150.5

17.29

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.165 ± 0.307

0.372 ± 0.105

6.909 ± 0.479

7.919 ± 0.528

4.092 ± 0.379

4.704 ± 0.316

1.648 ± 0.212

7.388 ± 0.373

8.716 ± 0.457

9.912 ± 0.421

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.525 ± 0.196

5.873 ± 0.322

2.153 ± 0.298

3.747 ± 0.397

4.757 ± 0.415

6.272 ± 0.363

6.298 ± 0.523

5.448 ± 0.418

0.797 ± 0.121

4.305 ± 0.311
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski