Paracoccus sp. DMF

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; Rhodobacteraceae; Paracoccus; unclassified Paracoccus

Average proteome isoelectric point is 6.49

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3948 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4Y8NX12|A0A4Y8NX12_9RHOB Glycerol-3-phosphate acyltransferase OS=Paracoccus sp. DMF OX=400837 GN=plsY PE=3 SV=1
MM1 pKa = 7.44KK2 pKa = 10.19KK3 pKa = 10.41VLFATTALVMTAGVAAAEE21 pKa = 4.42VAVSGDD27 pKa = 2.72GRR29 pKa = 11.84MGMIYY34 pKa = 10.3DD35 pKa = 3.98GNDD38 pKa = 3.0AQFSSRR44 pKa = 11.84ARR46 pKa = 11.84VTFTLTGEE54 pKa = 4.16SDD56 pKa = 3.06AGLSFGGAFRR66 pKa = 11.84VDD68 pKa = 3.32QEE70 pKa = 4.01SDD72 pKa = 3.42YY73 pKa = 10.06TAGYY77 pKa = 9.85AGSEE81 pKa = 3.9RR82 pKa = 11.84SAARR86 pKa = 11.84GTAGAVWISGTYY98 pKa = 10.15GKK100 pKa = 10.88LSMGDD105 pKa = 3.25VVGAAEE111 pKa = 4.24AAIGDD116 pKa = 4.28LPEE119 pKa = 4.2VGYY122 pKa = 9.54TDD124 pKa = 4.57GEE126 pKa = 4.17FGGDD130 pKa = 3.07IEE132 pKa = 4.83EE133 pKa = 4.2INYY136 pKa = 10.09LVGDD140 pKa = 4.77GEE142 pKa = 5.12NEE144 pKa = 4.15DD145 pKa = 3.56QGPTILYY152 pKa = 8.67EE153 pKa = 3.97YY154 pKa = 9.14TVNNISFFASATDD167 pKa = 3.81GSNNSTYY174 pKa = 10.81GVAGSDD180 pKa = 3.43EE181 pKa = 4.85GEE183 pKa = 4.41DD184 pKa = 3.66VPSDD188 pKa = 3.44SVAYY192 pKa = 9.89SLAVKK197 pKa = 10.77YY198 pKa = 10.3EE199 pKa = 3.84GSNWWAALSYY209 pKa = 11.02SEE211 pKa = 5.31ADD213 pKa = 4.07VYY215 pKa = 11.68DD216 pKa = 4.97LDD218 pKa = 4.02PTSTSVTASASEE230 pKa = 3.83LALAGGASFNNFSVMATYY248 pKa = 10.82LKK250 pKa = 10.65YY251 pKa = 10.49DD252 pKa = 3.6DD253 pKa = 5.32RR254 pKa = 11.84FVSGPVLGDD263 pKa = 3.52DD264 pKa = 3.71EE265 pKa = 5.98FIVGGFAVDD274 pKa = 3.35ATLEE278 pKa = 4.12EE279 pKa = 4.68TIGLGATYY287 pKa = 10.68QMDD290 pKa = 5.34AILVEE295 pKa = 3.99GFYY298 pKa = 10.93RR299 pKa = 11.84KK300 pKa = 10.02DD301 pKa = 3.19KK302 pKa = 10.9YY303 pKa = 11.5DD304 pKa = 4.57LIGGGSEE311 pKa = 4.41SYY313 pKa = 11.3DD314 pKa = 3.44SFGIGADD321 pKa = 3.47YY322 pKa = 11.21DD323 pKa = 4.05LGGGAVLAGGIIDD336 pKa = 4.38TDD338 pKa = 3.96YY339 pKa = 11.85LDD341 pKa = 4.77DD342 pKa = 3.81TVADD346 pKa = 3.47IGVKK350 pKa = 10.55FKK352 pKa = 11.06FF353 pKa = 3.75

Molecular weight: 36.73 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4Y8P5P5|A0A4Y8P5P5_9RHOB ABC-F family ATP-binding cassette domain-containing protein (Fragment) OS=Paracoccus sp. DMF OX=400837 GN=E3D03_01325 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.42RR3 pKa = 11.84TYY5 pKa = 10.34QPNKK9 pKa = 8.21RR10 pKa = 11.84KK11 pKa = 9.62RR12 pKa = 11.84SKK14 pKa = 9.59VHH16 pKa = 5.83GFRR19 pKa = 11.84SRR21 pKa = 11.84MSSPNGRR28 pKa = 11.84NVLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 9.05GRR39 pKa = 11.84KK40 pKa = 8.7VLSAA44 pKa = 4.05

Molecular weight: 5.25 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3948

0

3948

905382

20

1702

229.3

25.13

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.336 ± 0.058

0.859 ± 0.012

5.679 ± 0.033

6.15 ± 0.043

3.892 ± 0.032

8.456 ± 0.037

2.06 ± 0.02

5.757 ± 0.034

3.93 ± 0.043

9.938 ± 0.048

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.802 ± 0.02

2.949 ± 0.028

4.859 ± 0.03

3.356 ± 0.023

6.632 ± 0.049

5.246 ± 0.032

5.172 ± 0.036

6.971 ± 0.033

1.433 ± 0.021

2.52 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski