Nasonia vitripennis (Parasitic wasp)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Protostomia; Ecdysozoa; Panarthropoda; Arthropoda; Mandibulata; Pancrustacea; Hexapoda; Insecta; Dicondylia; Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita; Parasitoida; Chalcidoidea; Pteromalidae; Pteromalinae;

Average proteome isoelectric point is 6.72

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 24858 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A7M7Q6F1|A0A7M7Q6F1_NASVI Uncharacterized protein OS=Nasonia vitripennis OX=7425 PE=4 SV=1
MM1 pKa = 7.11VVLSNFLAPQEE12 pKa = 4.56GIRR15 pKa = 11.84HH16 pKa = 5.75EE17 pKa = 4.24EE18 pKa = 3.97QQTTLYY24 pKa = 10.7INDD27 pKa = 3.81RR28 pKa = 11.84EE29 pKa = 4.39VGKK32 pKa = 8.77GTLYY36 pKa = 9.3ITEE39 pKa = 4.71SLLSWVNNDD48 pKa = 2.62TRR50 pKa = 11.84QGFSLEE56 pKa = 4.09YY57 pKa = 10.05PHH59 pKa = 7.27ISLHH63 pKa = 6.32AISRR67 pKa = 11.84DD68 pKa = 3.7EE69 pKa = 3.97QVHH72 pKa = 5.92PRR74 pKa = 11.84QCLYY78 pKa = 11.54VMVDD82 pKa = 3.46AKK84 pKa = 11.32VDD86 pKa = 4.29FPDD89 pKa = 4.87SPTLQSNNDD98 pKa = 3.57SNEE101 pKa = 4.19SNNTNEE107 pKa = 5.23KK108 pKa = 11.11NNDD111 pKa = 3.8DD112 pKa = 4.61NDD114 pKa = 4.36DD115 pKa = 4.78DD116 pKa = 6.51SDD118 pKa = 6.18DD119 pKa = 5.03DD120 pKa = 6.28DD121 pKa = 6.36SDD123 pKa = 4.63APITEE128 pKa = 4.15MRR130 pKa = 11.84FAPDD134 pKa = 3.15NTNSLDD140 pKa = 4.55AMFQAMNEE148 pKa = 4.3CQALHH153 pKa = 7.5PDD155 pKa = 3.93PQDD158 pKa = 3.37SFSDD162 pKa = 3.74AEE164 pKa = 3.83EE165 pKa = 5.32DD166 pKa = 3.31IYY168 pKa = 11.51EE169 pKa = 4.54DD170 pKa = 4.26AEE172 pKa = 4.19EE173 pKa = 5.61DD174 pKa = 3.45DD175 pKa = 3.95FEE177 pKa = 5.04YY178 pKa = 11.51YY179 pKa = 10.0EE180 pKa = 5.11AGAGDD185 pKa = 4.1APYY188 pKa = 9.93IIPAEE193 pKa = 4.07EE194 pKa = 3.8VGASHH199 pKa = 7.12NGTEE203 pKa = 4.29AEE205 pKa = 3.91EE206 pKa = 4.14AMEE209 pKa = 4.11VEE211 pKa = 4.47AGQFEE216 pKa = 4.63DD217 pKa = 5.56AEE219 pKa = 4.32EE220 pKa = 4.4DD221 pKa = 3.54PP222 pKa = 5.23

Molecular weight: 24.93 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A7M6W5W7|A0A7M6W5W7_NASVI Odorant receptor OS=Nasonia vitripennis OX=7425 PE=3 SV=1
MM1 pKa = 7.1RR2 pKa = 11.84AKK4 pKa = 9.12WRR6 pKa = 11.84KK7 pKa = 9.1KK8 pKa = 9.32RR9 pKa = 11.84MRR11 pKa = 11.84RR12 pKa = 11.84LKK14 pKa = 10.08RR15 pKa = 11.84KK16 pKa = 7.85RR17 pKa = 11.84RR18 pKa = 11.84KK19 pKa = 8.6MRR21 pKa = 11.84ARR23 pKa = 11.84SKK25 pKa = 11.11

Molecular weight: 3.4 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

13385

11473

24858

17866849

25

20151

718.8

80.45

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.866 ± 0.022

1.748 ± 0.017

5.505 ± 0.017

7.002 ± 0.029

3.322 ± 0.012

5.597 ± 0.021

2.497 ± 0.008

5.148 ± 0.013

6.391 ± 0.032

8.701 ± 0.024

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.192 ± 0.008

4.91 ± 0.015

5.407 ± 0.022

4.949 ± 0.024

5.548 ± 0.018

8.73 ± 0.025

5.732 ± 0.017

5.928 ± 0.014

0.973 ± 0.004

2.853 ± 0.011
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski