Vibrio phage pYD38-A

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Roufvirus; unclassified Roufvirus

Average proteome isoelectric point is 6.48

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 77 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|R9TRY7|R9TRY7_9CAUD Uncharacterized protein OS=Vibrio phage pYD38-A OX=754051 GN=VPRG_00042 PE=4 SV=1
MM1 pKa = 8.2IIEE4 pKa = 4.3EE5 pKa = 4.49TEE7 pKa = 3.79NAYY10 pKa = 10.86DD11 pKa = 3.44MHH13 pKa = 5.74VTEE16 pKa = 5.67QEE18 pKa = 4.14LDD20 pKa = 3.51DD21 pKa = 4.11GTPVVEE27 pKa = 4.88LYY29 pKa = 10.12QVNGSGVEE37 pKa = 4.02SFIGIDD43 pKa = 3.24KK44 pKa = 10.25HH45 pKa = 6.49QAAQLII51 pKa = 4.22

Molecular weight:
5.68 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|R9TRE3|R9TRE3_9CAUD Uncharacterized protein OS=Vibrio phage pYD38-A OX=754051 GN=VPRG_00025 PE=4 SV=1
MM1 pKa = 7.41SKK3 pKa = 10.93ASTLHH8 pKa = 5.51EE9 pKa = 5.45LIMADD14 pKa = 2.86IRR16 pKa = 11.84EE17 pKa = 4.41SNARR21 pKa = 11.84ADD23 pKa = 3.64WQRR26 pKa = 11.84NQPDD30 pKa = 3.54RR31 pKa = 11.84RR32 pKa = 11.84TLKK35 pKa = 10.54QKK37 pKa = 9.69LHH39 pKa = 6.18PKK41 pKa = 9.53RR42 pKa = 11.84RR43 pKa = 11.84RR44 pKa = 11.84PNRR47 pKa = 11.84KK48 pKa = 7.81RR49 pKa = 11.84DD50 pKa = 3.4YY51 pKa = 10.89RR52 pKa = 11.84RR53 pKa = 11.84DD54 pKa = 3.23RR55 pKa = 11.84VLRR58 pKa = 11.84KK59 pKa = 10.12LCDD62 pKa = 3.17IQMKK66 pKa = 9.79FMIKK70 pKa = 10.14EE71 pKa = 4.14AMNEE75 pKa = 4.28TNDD78 pKa = 3.46EE79 pKa = 4.18

Molecular weight:
9.74 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

77

0

77

14315

51

1097

185.9

20.71

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.046 ± 0.788

1.271 ± 0.138

6.161 ± 0.296

6.518 ± 0.278

3.185 ± 0.202

7.118 ± 0.224

1.746 ± 0.201

6.196 ± 0.171

6.35 ± 0.361

7.328 ± 0.227

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.955 ± 0.17

4.897 ± 0.297

3.507 ± 0.241

4.024 ± 0.317

5.176 ± 0.273

6.574 ± 0.308

5.868 ± 0.447

6.846 ± 0.244

1.614 ± 0.138

3.619 ± 0.237

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski