alpha proteobacterium Q-1

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; unclassified Alphaproteobacteria

Average proteome isoelectric point is 6.33

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2787 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A061QKP8|A0A061QKP8_9PROT DNA mismatch repair protein MutS OS=alpha proteobacterium Q-1 OX=1492281 GN=mutS PE=3 SV=1
MM1 pKa = 7.37TYY3 pKa = 10.66VVTEE7 pKa = 3.87ACIKK11 pKa = 10.42CKK13 pKa = 10.08YY14 pKa = 7.47TDD16 pKa = 3.85CVEE19 pKa = 4.15VCPVDD24 pKa = 4.15CFYY27 pKa = 11.35EE28 pKa = 4.72GDD30 pKa = 3.56NMLVINPNEE39 pKa = 4.6CIDD42 pKa = 4.23CGVCEE47 pKa = 4.44PEE49 pKa = 4.82CPAEE53 pKa = 5.13AILPDD58 pKa = 3.99TEE60 pKa = 6.1DD61 pKa = 4.41GLEE64 pKa = 4.02KK65 pKa = 10.11WLEE68 pKa = 3.91LNADD72 pKa = 4.12YY73 pKa = 11.14SEE75 pKa = 4.09KK76 pKa = 10.13WPNITTKK83 pKa = 10.53KK84 pKa = 10.05EE85 pKa = 3.94PPADD89 pKa = 4.09ADD91 pKa = 3.63DD92 pKa = 5.3HH93 pKa = 7.57KK94 pKa = 11.62DD95 pKa = 3.31EE96 pKa = 5.71DD97 pKa = 3.99GKK99 pKa = 11.02FDD101 pKa = 3.65KK102 pKa = 10.85FFSAEE107 pKa = 3.95PGAGDD112 pKa = 3.38

Molecular weight: 12.49 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A061QCU4|A0A061QCU4_9PROT Energy-dependent translational throttle protein EttA OS=alpha proteobacterium Q-1 OX=1492281 GN=ettA PE=3 SV=1
MM1 pKa = 7.55SFTANTIRR9 pKa = 11.84NTTSAARR16 pKa = 11.84ISIRR20 pKa = 11.84RR21 pKa = 11.84NRR23 pKa = 11.84RR24 pKa = 11.84RR25 pKa = 11.84QRR27 pKa = 11.84YY28 pKa = 7.37QRR30 pKa = 11.84AAA32 pKa = 3.24

Molecular weight: 3.82 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2787

0

2787

927933

29

1632

333.0

36.38

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.617 ± 0.056

0.798 ± 0.013

6.596 ± 0.034

5.463 ± 0.041

3.918 ± 0.036

8.251 ± 0.041

2.325 ± 0.025

5.874 ± 0.037

3.585 ± 0.036

10.621 ± 0.055

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.634 ± 0.021

2.735 ± 0.031

5.082 ± 0.032

3.652 ± 0.026

6.837 ± 0.037

5.856 ± 0.03

4.559 ± 0.032

6.162 ± 0.038

1.207 ± 0.019

2.228 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski