Tobacco bushy top virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Tolucaviricetes; Tolivirales; Tombusviridae; Calvusvirinae; Umbravirus

Average proteome isoelectric point is 7.39

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q8V3U5|Q8V3U5_9TOMB RNA-directed RNA polymerase OS=Tobacco bushy top virus OX=184020 PE=4 SV=3
MM1 pKa = 7.52EE2 pKa = 6.46FINKK6 pKa = 9.07IKK8 pKa = 10.48QLLAMNFKK16 pKa = 9.9PSKK19 pKa = 9.99GVMSRR24 pKa = 11.84EE25 pKa = 3.96EE26 pKa = 3.8LRR28 pKa = 11.84EE29 pKa = 4.02AFDD32 pKa = 4.04PTWEE36 pKa = 4.09LLITQARR43 pKa = 11.84VTNEE47 pKa = 3.41VSRR50 pKa = 11.84QCEE53 pKa = 3.62DD54 pKa = 3.1WYY56 pKa = 10.22TLAVPTTYY64 pKa = 10.78RR65 pKa = 11.84LPEE68 pKa = 4.09LAVEE72 pKa = 4.07EE73 pKa = 4.15AVRR76 pKa = 11.84EE77 pKa = 4.0KK78 pKa = 11.16NIARR82 pKa = 11.84EE83 pKa = 4.21VAVKK87 pKa = 10.13CPPEE91 pKa = 4.71DD92 pKa = 5.12PIPAIPQGSQPVPLVMSSEE111 pKa = 4.37RR112 pKa = 11.84SSQDD116 pKa = 2.89AEE118 pKa = 4.19RR119 pKa = 11.84EE120 pKa = 4.04ALDD123 pKa = 4.99EE124 pKa = 4.1IWGLPTPEE132 pKa = 4.02SHH134 pKa = 7.63PLPKK138 pKa = 10.38YY139 pKa = 9.92FEE141 pKa = 4.16RR142 pKa = 11.84RR143 pKa = 11.84YY144 pKa = 9.41QALARR149 pKa = 11.84TCEE152 pKa = 3.98KK153 pKa = 10.65DD154 pKa = 3.35YY155 pKa = 11.48SNWQIVPYY163 pKa = 9.73TGGPRR168 pKa = 11.84VLRR171 pKa = 11.84EE172 pKa = 3.78EE173 pKa = 5.01DD174 pKa = 3.98VLPMASGVIPLPPPPPKK191 pKa = 10.43ASVIGAVLGLITRR204 pKa = 11.84LTKK207 pKa = 10.53VAGEE211 pKa = 4.26VKK213 pKa = 10.34SRR215 pKa = 11.84LSVPPRR221 pKa = 11.84EE222 pKa = 4.44PSPTCIGLEE231 pKa = 4.1QVAGEE236 pKa = 3.93PMGYY240 pKa = 9.23MNAHH244 pKa = 5.48SVAMEE249 pKa = 3.65LRR251 pKa = 11.84ARR253 pKa = 11.84YY254 pKa = 8.58GVQPATAANLQLGNRR269 pKa = 11.84VARR272 pKa = 11.84EE273 pKa = 3.81ILEE276 pKa = 4.33KK277 pKa = 10.71QCGATRR283 pKa = 11.84DD284 pKa = 3.54MVFILGHH291 pKa = 6.87LATTLWFTPTMVDD304 pKa = 4.55LALQCGPKK312 pKa = 10.33DD313 pKa = 3.54FCC315 pKa = 6.59

Molecular weight: 35.04 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q8B8Q9|Q8B8Q9_9TOMB Uncharacterized protein OS=Tobacco bushy top virus OX=184020 PE=4 SV=1
MM1 pKa = 6.99STIINVNTHH10 pKa = 4.46PQGRR14 pKa = 11.84YY15 pKa = 7.21EE16 pKa = 4.07RR17 pKa = 11.84GAAQRR22 pKa = 11.84SIRR25 pKa = 11.84GSDD28 pKa = 3.1VKK30 pKa = 10.53GARR33 pKa = 11.84GIKK36 pKa = 9.26PRR38 pKa = 11.84RR39 pKa = 11.84SNPPSGRR46 pKa = 11.84KK47 pKa = 8.75QGGNDD52 pKa = 3.21PTAATQKK59 pKa = 10.13SRR61 pKa = 11.84KK62 pKa = 8.53NRR64 pKa = 11.84QRR66 pKa = 11.84PEE68 pKa = 3.86TVPPHH73 pKa = 5.35EE74 pKa = 4.34THH76 pKa = 6.59GGTFIHH82 pKa = 7.24RR83 pKa = 11.84EE84 pKa = 3.98GCGGVHH90 pKa = 6.65APHH93 pKa = 7.76PGRR96 pKa = 11.84CWRR99 pKa = 11.84RR100 pKa = 11.84GRR102 pKa = 11.84NLGPRR107 pKa = 11.84QHH109 pKa = 6.5ATTPKK114 pKa = 10.07QRR116 pKa = 11.84WAPRR120 pKa = 11.84KK121 pKa = 9.67ISDD124 pKa = 3.36EE125 pKa = 3.68RR126 pKa = 11.84RR127 pKa = 11.84AQVDD131 pKa = 4.17SILSPLLDD139 pKa = 3.62TLKK142 pKa = 10.83LSSEE146 pKa = 4.18GGAPEE151 pKa = 4.5LLHH154 pKa = 6.9CIGVLRR160 pKa = 11.84RR161 pKa = 11.84EE162 pKa = 3.97LRR164 pKa = 11.84RR165 pKa = 11.84RR166 pKa = 11.84RR167 pKa = 11.84EE168 pKa = 3.86PVQPVHH174 pKa = 6.58NVGTEE179 pKa = 3.69DD180 pKa = 3.49RR181 pKa = 11.84VRR183 pKa = 11.84SAQLSDD189 pKa = 3.99EE190 pKa = 4.63APQSNANLQTSGEE203 pKa = 4.32GQSVLPNPDD212 pKa = 2.82SGGTEE217 pKa = 3.72KK218 pKa = 11.19CNVEE222 pKa = 4.49SVCNNCDD229 pKa = 3.95RR230 pKa = 11.84APTTNKK236 pKa = 8.12WW237 pKa = 3.04

Molecular weight: 26.1 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

1661

237

865

415.3

46.4

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.285 ± 0.507

2.288 ± 0.224

4.094 ± 0.538

7.164 ± 0.54

2.95 ± 0.716

6.743 ± 0.843

2.408 ± 0.511

4.275 ± 0.212

4.877 ± 0.407

8.308 ± 0.725

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.529 ± 0.507

3.492 ± 0.582

8.549 ± 0.694

3.612 ± 0.537

7.766 ± 1.151

6.201 ± 0.606

5.178 ± 0.44

8.128 ± 0.672

1.626 ± 0.234

2.529 ± 0.509
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski