Amino acid dipepetide frequency for Streptomyces phage Bing

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
10.894AlaAla: 10.894 ± 1.466
0.287AlaCys: 0.287 ± 0.138
5.218AlaAsp: 5.218 ± 0.546
7.339AlaGlu: 7.339 ± 0.61
3.096AlaPhe: 3.096 ± 0.382
7.339AlaGly: 7.339 ± 1.32
1.261AlaHis: 1.261 ± 0.232
5.39AlaIle: 5.39 ± 0.789
7.339AlaLys: 7.339 ± 0.758
7.511AlaLeu: 7.511 ± 1.279
3.325AlaMet: 3.325 ± 0.426
3.153AlaAsn: 3.153 ± 0.429
3.44AlaPro: 3.44 ± 0.559
3.039AlaGln: 3.039 ± 0.36
4.644AlaArg: 4.644 ± 0.531
3.899AlaSer: 3.899 ± 0.573
5.619AlaThr: 5.619 ± 0.509
6.766AlaVal: 6.766 ± 0.597
1.376AlaTrp: 1.376 ± 0.36
3.039AlaTyr: 3.039 ± 0.415
0.0AlaXaa: 0.0 ± 0.0
Cys
0.344CysAla: 0.344 ± 0.165
0.057CysCys: 0.057 ± 0.052
0.459CysAsp: 0.459 ± 0.154
0.344CysGlu: 0.344 ± 0.155
0.115CysPhe: 0.115 ± 0.085
0.401CysGly: 0.401 ± 0.154
0.0CysHis: 0.0 ± 0.0
0.229CysIle: 0.229 ± 0.122
0.401CysLys: 0.401 ± 0.146
0.688CysLeu: 0.688 ± 0.215
0.0CysMet: 0.0 ± 0.0
0.115CysAsn: 0.115 ± 0.107
0.115CysPro: 0.115 ± 0.097
0.0CysGln: 0.0 ± 0.0
0.172CysArg: 0.172 ± 0.101
0.229CysSer: 0.229 ± 0.117
0.287CysThr: 0.287 ± 0.111
0.459CysVal: 0.459 ± 0.181
0.115CysTrp: 0.115 ± 0.076
0.057CysTyr: 0.057 ± 0.061
0.0CysXaa: 0.0 ± 0.0
Asp
5.619AspAla: 5.619 ± 0.58
0.401AspCys: 0.401 ± 0.155
3.44AspAsp: 3.44 ± 0.513
5.906AspGlu: 5.906 ± 0.769
3.268AspPhe: 3.268 ± 0.519
5.848AspGly: 5.848 ± 0.654
0.917AspHis: 0.917 ± 0.259
3.383AspIle: 3.383 ± 0.522
2.809AspLys: 2.809 ± 0.548
4.358AspLeu: 4.358 ± 0.507
1.605AspMet: 1.605 ± 0.299
1.949AspAsn: 1.949 ± 0.339
3.096AspPro: 3.096 ± 0.514
2.007AspGln: 2.007 ± 0.304
2.867AspArg: 2.867 ± 0.487
3.039AspSer: 3.039 ± 0.48
3.67AspThr: 3.67 ± 0.5
4.587AspVal: 4.587 ± 0.424
0.917AspTrp: 0.917 ± 0.206
1.892AspTyr: 1.892 ± 0.326
0.0AspXaa: 0.0 ± 0.0
Glu
5.218GluAla: 5.218 ± 0.707
0.229GluCys: 0.229 ± 0.117
4.472GluAsp: 4.472 ± 0.752
6.422GluGlu: 6.422 ± 0.893
2.809GluPhe: 2.809 ± 0.429
4.874GluGly: 4.874 ± 0.557
1.548GluHis: 1.548 ± 0.347
3.784GluIle: 3.784 ± 0.415
5.906GluLys: 5.906 ± 0.626
5.447GluLeu: 5.447 ± 0.568
2.351GluMet: 2.351 ± 0.332
2.351GluAsn: 2.351 ± 0.438
3.096GluPro: 3.096 ± 0.523
2.981GluGln: 2.981 ± 0.38
4.3GluArg: 4.3 ± 0.45
2.981GluSer: 2.981 ± 0.383
4.186GluThr: 4.186 ± 0.471
4.988GluVal: 4.988 ± 0.523
1.548GluTrp: 1.548 ± 0.352
1.777GluTyr: 1.777 ± 0.385
0.0GluXaa: 0.0 ± 0.0
Phe
3.383PheAla: 3.383 ± 0.457
0.287PheCys: 0.287 ± 0.128
3.153PheAsp: 3.153 ± 0.363
1.892PheGlu: 1.892 ± 0.336
1.147PhePhe: 1.147 ± 0.228
2.924PheGly: 2.924 ± 0.463
0.631PheHis: 0.631 ± 0.227
2.236PheIle: 2.236 ± 0.416
2.236PheLys: 2.236 ± 0.403
2.064PheLeu: 2.064 ± 0.354
0.803PheMet: 0.803 ± 0.203
1.376PheAsn: 1.376 ± 0.255
1.376PhePro: 1.376 ± 0.297
1.892PheGln: 1.892 ± 0.324
2.179PheArg: 2.179 ± 0.303
2.351PheSer: 2.351 ± 0.423
2.465PheThr: 2.465 ± 0.456
2.351PheVal: 2.351 ± 0.382
0.631PheTrp: 0.631 ± 0.193
1.433PheTyr: 1.433 ± 0.394
0.0PheXaa: 0.0 ± 0.0
Gly
7.11GlyAla: 7.11 ± 0.833
0.229GlyCys: 0.229 ± 0.111
4.644GlyAsp: 4.644 ± 0.408
4.128GlyGlu: 4.128 ± 0.47
2.752GlyPhe: 2.752 ± 0.432
4.759GlyGly: 4.759 ± 0.47
1.261GlyHis: 1.261 ± 0.273
4.3GlyIle: 4.3 ± 0.885
4.988GlyLys: 4.988 ± 0.735
5.848GlyLeu: 5.848 ± 1.589
2.523GlyMet: 2.523 ± 0.417
2.695GlyAsn: 2.695 ± 0.428
2.293GlyPro: 2.293 ± 0.337
2.064GlyGln: 2.064 ± 0.404
3.325GlyArg: 3.325 ± 0.318
5.332GlySer: 5.332 ± 0.618
6.135GlyThr: 6.135 ± 0.647
7.167GlyVal: 7.167 ± 0.98
1.491GlyTrp: 1.491 ± 0.339
2.924GlyTyr: 2.924 ± 0.554
0.0GlyXaa: 0.0 ± 0.0
His
1.491HisAla: 1.491 ± 0.362
0.172HisCys: 0.172 ± 0.088
0.745HisAsp: 0.745 ± 0.224
0.975HisGlu: 0.975 ± 0.332
0.631HisPhe: 0.631 ± 0.195
1.491HisGly: 1.491 ± 0.415
0.631HisHis: 0.631 ± 0.214
1.777HisIle: 1.777 ± 0.369
0.917HisLys: 0.917 ± 0.26
1.491HisLeu: 1.491 ± 0.313
0.459HisMet: 0.459 ± 0.149
0.573HisAsn: 0.573 ± 0.17
1.147HisPro: 1.147 ± 0.292
0.459HisGln: 0.459 ± 0.185
0.688HisArg: 0.688 ± 0.2
0.917HisSer: 0.917 ± 0.265
0.516HisThr: 0.516 ± 0.173
1.089HisVal: 1.089 ± 0.279
0.172HisTrp: 0.172 ± 0.104
1.089HisTyr: 1.089 ± 0.192
0.0HisXaa: 0.0 ± 0.0
Ile
4.816IleAla: 4.816 ± 0.616
0.344IleCys: 0.344 ± 0.151
3.784IleAsp: 3.784 ± 0.428
3.612IleGlu: 3.612 ± 0.531
2.007IlePhe: 2.007 ± 0.334
3.727IleGly: 3.727 ± 0.704
1.147IleHis: 1.147 ± 0.297
2.064IleIle: 2.064 ± 0.476
3.842IleLys: 3.842 ± 0.567
4.243IleLeu: 4.243 ± 0.477
0.975IleMet: 0.975 ± 0.191
2.351IleAsn: 2.351 ± 0.334
2.924IlePro: 2.924 ± 0.418
1.777IleGln: 1.777 ± 0.281
2.523IleArg: 2.523 ± 0.429
3.44IleSer: 3.44 ± 0.598
3.842IleThr: 3.842 ± 0.446
3.67IleVal: 3.67 ± 0.431
0.516IleTrp: 0.516 ± 0.18
1.319IleTyr: 1.319 ± 0.262
0.0IleXaa: 0.0 ± 0.0
Lys
6.135LysAla: 6.135 ± 0.558
0.115LysCys: 0.115 ± 0.077
3.727LysAsp: 3.727 ± 0.492
3.612LysGlu: 3.612 ± 0.631
2.179LysPhe: 2.179 ± 0.276
4.243LysGly: 4.243 ± 0.811
1.376LysHis: 1.376 ± 0.361
2.924LysIle: 2.924 ± 0.575
4.415LysLys: 4.415 ± 0.626
6.823LysLeu: 6.823 ± 0.732
2.236LysMet: 2.236 ± 0.382
3.211LysAsn: 3.211 ± 0.494
2.523LysPro: 2.523 ± 0.45
2.752LysGln: 2.752 ± 0.292
3.555LysArg: 3.555 ± 0.691
4.243LysSer: 4.243 ± 0.567
4.874LysThr: 4.874 ± 0.704
4.53LysVal: 4.53 ± 0.757
1.204LysTrp: 1.204 ± 0.301
2.408LysTyr: 2.408 ± 0.327
0.0LysXaa: 0.0 ± 0.0
Leu
8.772LeuAla: 8.772 ± 1.205
0.344LeuCys: 0.344 ± 0.126
5.676LeuAsp: 5.676 ± 0.641
5.906LeuGlu: 5.906 ± 0.598
2.752LeuPhe: 2.752 ± 0.53
6.422LeuGly: 6.422 ± 1.157
1.261LeuHis: 1.261 ± 0.251
3.842LeuIle: 3.842 ± 0.63
4.243LeuLys: 4.243 ± 0.506
5.791LeuLeu: 5.791 ± 0.743
2.695LeuMet: 2.695 ± 0.354
3.096LeuAsn: 3.096 ± 0.348
2.293LeuPro: 2.293 ± 0.273
2.523LeuGln: 2.523 ± 0.371
4.014LeuArg: 4.014 ± 0.539
4.759LeuSer: 4.759 ± 0.511
5.218LeuThr: 5.218 ± 0.837
5.504LeuVal: 5.504 ± 0.583
0.803LeuTrp: 0.803 ± 0.216
2.465LeuTyr: 2.465 ± 0.363
0.0LeuXaa: 0.0 ± 0.0
Met
2.236MetAla: 2.236 ± 0.336
0.115MetCys: 0.115 ± 0.071
1.835MetAsp: 1.835 ± 0.341
1.835MetGlu: 1.835 ± 0.367
0.917MetPhe: 0.917 ± 0.218
1.663MetGly: 1.663 ± 0.268
0.172MetHis: 0.172 ± 0.091
1.089MetIle: 1.089 ± 0.293
2.007MetLys: 2.007 ± 0.411
2.293MetLeu: 2.293 ± 0.407
0.401MetMet: 0.401 ± 0.191
1.204MetAsn: 1.204 ± 0.275
1.032MetPro: 1.032 ± 0.238
0.975MetGln: 0.975 ± 0.239
1.491MetArg: 1.491 ± 0.311
1.835MetSer: 1.835 ± 0.362
2.121MetThr: 2.121 ± 0.341
2.064MetVal: 2.064 ± 0.273
0.459MetTrp: 0.459 ± 0.133
0.917MetTyr: 0.917 ± 0.27
0.0MetXaa: 0.0 ± 0.0
Asn
4.186AsnAla: 4.186 ± 0.53
0.229AsnCys: 0.229 ± 0.148
2.523AsnAsp: 2.523 ± 0.375
3.096AsnGlu: 3.096 ± 0.31
1.376AsnPhe: 1.376 ± 0.355
3.44AsnGly: 3.44 ± 0.506
0.631AsnHis: 0.631 ± 0.233
1.892AsnIle: 1.892 ± 0.308
2.523AsnLys: 2.523 ± 0.351
2.981AsnLeu: 2.981 ± 0.42
0.745AsnMet: 0.745 ± 0.186
2.007AsnAsn: 2.007 ± 0.418
2.351AsnPro: 2.351 ± 0.381
1.319AsnGln: 1.319 ± 0.232
2.408AsnArg: 2.408 ± 0.417
2.236AsnSer: 2.236 ± 0.3
1.892AsnThr: 1.892 ± 0.344
2.752AsnVal: 2.752 ± 0.446
0.516AsnTrp: 0.516 ± 0.21
1.433AsnTyr: 1.433 ± 0.304
0.0AsnXaa: 0.0 ± 0.0
Pro
2.752ProAla: 2.752 ± 0.442
0.344ProCys: 0.344 ± 0.151
2.465ProAsp: 2.465 ± 0.423
3.612ProGlu: 3.612 ± 0.735
1.261ProPhe: 1.261 ± 0.243
3.383ProGly: 3.383 ± 0.548
0.86ProHis: 0.86 ± 0.24
1.605ProIle: 1.605 ± 0.304
3.268ProLys: 3.268 ± 0.466
2.924ProLeu: 2.924 ± 0.498
0.516ProMet: 0.516 ± 0.177
1.663ProAsn: 1.663 ± 0.326
1.72ProPro: 1.72 ± 0.279
0.975ProGln: 0.975 ± 0.223
2.064ProArg: 2.064 ± 0.439
2.695ProSer: 2.695 ± 0.46
3.039ProThr: 3.039 ± 0.604
3.211ProVal: 3.211 ± 0.377
0.287ProTrp: 0.287 ± 0.119
1.376ProTyr: 1.376 ± 0.393
0.0ProXaa: 0.0 ± 0.0
Gln
3.268GlnAla: 3.268 ± 0.414
0.172GlnCys: 0.172 ± 0.11
1.605GlnAsp: 1.605 ± 0.327
2.121GlnGlu: 2.121 ± 0.357
1.147GlnPhe: 1.147 ± 0.223
2.867GlnGly: 2.867 ± 0.381
0.803GlnHis: 0.803 ± 0.222
2.236GlnIle: 2.236 ± 0.328
1.949GlnLys: 1.949 ± 0.323
3.211GlnLeu: 3.211 ± 0.477
0.917GlnMet: 0.917 ± 0.187
1.491GlnAsn: 1.491 ± 0.298
1.376GlnPro: 1.376 ± 0.22
1.204GlnGln: 1.204 ± 0.255
2.007GlnArg: 2.007 ± 0.369
1.949GlnSer: 1.949 ± 0.34
1.892GlnThr: 1.892 ± 0.388
1.835GlnVal: 1.835 ± 0.261
0.172GlnTrp: 0.172 ± 0.098
1.032GlnTyr: 1.032 ± 0.191
0.0GlnXaa: 0.0 ± 0.0
Arg
4.128ArgAla: 4.128 ± 0.679
0.287ArgCys: 0.287 ± 0.129
3.039ArgAsp: 3.039 ± 0.558
3.498ArgGlu: 3.498 ± 0.485
1.376ArgPhe: 1.376 ± 0.265
2.867ArgGly: 2.867 ± 0.387
0.975ArgHis: 0.975 ± 0.267
2.408ArgIle: 2.408 ± 0.468
3.842ArgLys: 3.842 ± 0.515
4.186ArgLeu: 4.186 ± 0.581
1.949ArgMet: 1.949 ± 0.287
3.039ArgAsn: 3.039 ± 0.38
1.72ArgPro: 1.72 ± 0.392
1.835ArgGln: 1.835 ± 0.361
3.784ArgArg: 3.784 ± 0.614
3.153ArgSer: 3.153 ± 0.475
3.784ArgThr: 3.784 ± 0.555
2.924ArgVal: 2.924 ± 0.44
0.745ArgTrp: 0.745 ± 0.248
1.605ArgTyr: 1.605 ± 0.253
0.0ArgXaa: 0.0 ± 0.0
Ser
5.218SerAla: 5.218 ± 0.657
0.172SerCys: 0.172 ± 0.106
3.325SerAsp: 3.325 ± 0.437
3.784SerGlu: 3.784 ± 0.39
2.179SerPhe: 2.179 ± 0.282
5.619SerGly: 5.619 ± 0.531
1.147SerHis: 1.147 ± 0.257
3.555SerIle: 3.555 ± 0.381
4.3SerLys: 4.3 ± 0.491
4.3SerLeu: 4.3 ± 0.534
1.376SerMet: 1.376 ± 0.254
2.064SerAsn: 2.064 ± 0.346
2.121SerPro: 2.121 ± 0.344
2.179SerGln: 2.179 ± 0.364
2.809SerArg: 2.809 ± 0.464
4.186SerSer: 4.186 ± 0.591
3.325SerThr: 3.325 ± 0.427
4.186SerVal: 4.186 ± 0.419
0.917SerTrp: 0.917 ± 0.238
2.408SerTyr: 2.408 ± 0.446
0.0SerXaa: 0.0 ± 0.0
Thr
6.479ThrAla: 6.479 ± 0.781
0.229ThrCys: 0.229 ± 0.128
3.784ThrAsp: 3.784 ± 0.596
4.358ThrGlu: 4.358 ± 0.588
2.809ThrPhe: 2.809 ± 0.402
5.963ThrGly: 5.963 ± 0.603
1.204ThrHis: 1.204 ± 0.264
3.842ThrIle: 3.842 ± 0.603
3.784ThrLys: 3.784 ± 0.408
5.447ThrLeu: 5.447 ± 0.612
1.319ThrMet: 1.319 ± 0.266
1.663ThrAsn: 1.663 ± 0.305
3.096ThrPro: 3.096 ± 0.502
1.835ThrGln: 1.835 ± 0.296
2.695ThrArg: 2.695 ± 0.325
3.612ThrSer: 3.612 ± 0.625
5.848ThrThr: 5.848 ± 0.791
4.988ThrVal: 4.988 ± 0.548
1.319ThrTrp: 1.319 ± 0.3
2.637ThrTyr: 2.637 ± 0.529
0.0ThrXaa: 0.0 ± 0.0
Val
7.339ValAla: 7.339 ± 0.585
0.172ValCys: 0.172 ± 0.087
4.358ValAsp: 4.358 ± 0.341
5.275ValGlu: 5.275 ± 0.632
2.523ValPhe: 2.523 ± 0.392
4.988ValGly: 4.988 ± 0.499
0.745ValHis: 0.745 ± 0.244
4.415ValIle: 4.415 ± 0.323
5.16ValLys: 5.16 ± 0.574
5.275ValLeu: 5.275 ± 0.698
1.835ValMet: 1.835 ± 0.363
3.612ValAsn: 3.612 ± 0.38
2.58ValPro: 2.58 ± 0.471
1.949ValGln: 1.949 ± 0.272
3.842ValArg: 3.842 ± 0.696
4.128ValSer: 4.128 ± 0.435
4.816ValThr: 4.816 ± 0.559
5.848ValVal: 5.848 ± 0.8
0.975ValTrp: 0.975 ± 0.24
2.981ValTyr: 2.981 ± 0.484
0.0ValXaa: 0.0 ± 0.0
Trp
1.663TrpAla: 1.663 ± 0.292
0.057TrpCys: 0.057 ± 0.055
1.032TrpAsp: 1.032 ± 0.22
1.032TrpGlu: 1.032 ± 0.2
0.573TrpPhe: 0.573 ± 0.176
0.917TrpGly: 0.917 ± 0.339
0.287TrpHis: 0.287 ± 0.126
0.516TrpIle: 0.516 ± 0.242
0.688TrpLys: 0.688 ± 0.243
1.032TrpLeu: 1.032 ± 0.222
0.287TrpMet: 0.287 ± 0.111
1.204TrpAsn: 1.204 ± 0.279
0.401TrpPro: 0.401 ± 0.13
0.172TrpGln: 0.172 ± 0.078
0.516TrpArg: 0.516 ± 0.185
1.319TrpSer: 1.319 ± 0.401
1.376TrpThr: 1.376 ± 0.314
1.147TrpVal: 1.147 ± 0.294
0.344TrpTrp: 0.344 ± 0.161
0.344TrpTyr: 0.344 ± 0.143
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.924TyrAla: 2.924 ± 0.606
0.344TyrCys: 0.344 ± 0.165
2.293TyrAsp: 2.293 ± 0.413
2.58TyrGlu: 2.58 ± 0.413
1.892TyrPhe: 1.892 ± 0.362
2.236TyrGly: 2.236 ± 0.383
0.573TyrHis: 0.573 ± 0.176
1.433TyrIle: 1.433 ± 0.32
2.465TyrLys: 2.465 ± 0.419
2.465TyrLeu: 2.465 ± 0.373
0.287TyrMet: 0.287 ± 0.178
1.72TyrAsn: 1.72 ± 0.26
1.319TyrPro: 1.319 ± 0.283
1.319TyrGln: 1.319 ± 0.269
1.261TyrArg: 1.261 ± 0.288
2.867TyrSer: 2.867 ± 0.408
2.007TyrThr: 2.007 ± 0.43
2.752TyrVal: 2.752 ± 0.394
0.401TyrTrp: 0.401 ± 0.166
1.433TyrTyr: 1.433 ± 0.333
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 87 proteins (17442 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski