Monosporascus ibericus

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; sordariomyceta; Sordariomycetes; Xylariomycetidae; Xylariales; Xylariales incertae sedis; Monosporascus

Average proteome isoelectric point is 6.41

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 11059 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4Q4SRJ8|A0A4Q4SRJ8_9PEZI FMN hydroxy acid dehydrogenase domain-containing protein OS=Monosporascus ibericus OX=155417 GN=DL764_011043 PE=3 SV=1
MM1 pKa = 7.83HH2 pKa = 6.65YY3 pKa = 7.03TTASFLAVLGLASAQRR19 pKa = 11.84LHH21 pKa = 6.01VVSVSGEE28 pKa = 3.98GDD30 pKa = 3.0WALKK34 pKa = 10.01FSPDD38 pKa = 3.64NIKK41 pKa = 10.65VPVGDD46 pKa = 4.19MIQFQFRR53 pKa = 11.84AGNHH57 pKa = 5.33SVVQSNFDD65 pKa = 3.78NPCEE69 pKa = 4.27PIKK72 pKa = 10.27MHH74 pKa = 6.55TGAEE78 pKa = 4.33GFYY81 pKa = 10.58SGYY84 pKa = 10.22QLVAASEE91 pKa = 4.16EE92 pKa = 4.03MDD94 pKa = 5.55LIPTYY99 pKa = 10.56TVMVQDD105 pKa = 4.81EE106 pKa = 4.75SPMWVYY112 pKa = 10.76CSQGQHH118 pKa = 5.89CQNGMVMVVNEE129 pKa = 3.88NTAANSSRR137 pKa = 11.84SLEE140 pKa = 4.11EE141 pKa = 3.78YY142 pKa = 10.61RR143 pKa = 11.84NLAAEE148 pKa = 4.63APDD151 pKa = 3.81NLAGDD156 pKa = 3.84VEE158 pKa = 4.95GIGGEE163 pKa = 4.24TGTTPEE169 pKa = 3.97PTEE172 pKa = 4.77PGDD175 pKa = 3.72EE176 pKa = 4.02TDD178 pKa = 3.94APSDD182 pKa = 3.81EE183 pKa = 4.63EE184 pKa = 4.58TPATPGDD191 pKa = 4.28DD192 pKa = 3.72DD193 pKa = 4.21TTTGDD198 pKa = 3.86DD199 pKa = 3.56TTGDD203 pKa = 3.54EE204 pKa = 4.46TLTPGSDD211 pKa = 3.15ATPTEE216 pKa = 4.31TADD219 pKa = 3.52GAASGTEE226 pKa = 3.96AADD229 pKa = 3.6EE230 pKa = 4.58ASTSVAQVGGGSMVTVTSPMGFFALAAAFFMLL262 pKa = 5.12

Molecular weight: 27.4 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4Q4T0V2|A0A4Q4T0V2_9PEZI t-SNARE coiled-coil homology domain-containing protein OS=Monosporascus ibericus OX=155417 GN=DL764_008454 PE=4 SV=1
MM1 pKa = 7.14MRR3 pKa = 11.84PFTVAVSAVRR13 pKa = 11.84TALSGAQSRR22 pKa = 11.84STIITTASKK31 pKa = 9.62RR32 pKa = 11.84TFSSLPQLRR41 pKa = 11.84PSILPPAHH49 pKa = 5.16GTVFRR54 pKa = 11.84PANNAVSRR62 pKa = 11.84VSLTPSVPAGATGPEE77 pKa = 4.1ALDD80 pKa = 4.18LVPKK84 pKa = 9.25TAITAHH90 pKa = 6.85PALAGVQIRR99 pKa = 11.84CGPRR103 pKa = 11.84PTMARR108 pKa = 11.84SSRR111 pKa = 11.84LIRR114 pKa = 11.84KK115 pKa = 8.73RR116 pKa = 11.84RR117 pKa = 11.84HH118 pKa = 5.22GFLSRR123 pKa = 11.84LRR125 pKa = 11.84TRR127 pKa = 11.84NGRR130 pKa = 11.84KK131 pKa = 8.2MLQRR135 pKa = 11.84RR136 pKa = 11.84KK137 pKa = 9.87DD138 pKa = 3.25KK139 pKa = 10.74KK140 pKa = 10.63RR141 pKa = 11.84SVLSMM146 pKa = 4.25

Molecular weight: 15.87 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

11059

0

11059

5384106

66

15764

486.9

53.69

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.241 ± 0.021

1.163 ± 0.009

5.91 ± 0.016

6.377 ± 0.022

3.535 ± 0.014

7.315 ± 0.023

2.327 ± 0.009

4.478 ± 0.015

4.621 ± 0.019

8.71 ± 0.025

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.111 ± 0.009

3.522 ± 0.013

6.22 ± 0.028

3.875 ± 0.017

6.579 ± 0.022

7.884 ± 0.027

5.775 ± 0.016

6.2 ± 0.016

1.448 ± 0.008

2.707 ± 0.011
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski