Erwinia phage vB_EhrS_49

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Feofaniavirus; Erwinia virus Eho49

Average proteome isoelectric point is 6.47

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 80 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4Y1NR55|A0A4Y1NR55_9CAUD Uncharacterized protein OS=Erwinia phage vB_EhrS_49 OX=2283026 GN=MZUP3_710 PE=4 SV=1
MM1 pKa = 7.73RR2 pKa = 11.84SVEE5 pKa = 4.15EE6 pKa = 3.84QCADD10 pKa = 3.77DD11 pKa = 4.97LADD14 pKa = 3.67VLDD17 pKa = 4.68SIEE20 pKa = 4.19GRR22 pKa = 11.84GEE24 pKa = 3.87EE25 pKa = 4.07PMLYY29 pKa = 10.27LLSYY33 pKa = 9.93LHH35 pKa = 7.23GYY37 pKa = 8.55LQGISVDD44 pKa = 3.55QEE46 pKa = 4.15NIPFVMDD53 pKa = 4.64PGAGGIRR60 pKa = 11.84VEE62 pKa = 5.19IIDD65 pKa = 5.79DD66 pKa = 3.44MTEE69 pKa = 3.95YY70 pKa = 9.4DD71 pKa = 3.33TSEE74 pKa = 4.06QARR77 pKa = 11.84LHH79 pKa = 5.85

Molecular weight: 8.86 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4Y1NR56|A0A4Y1NR56_9CAUD Uncharacterized protein OS=Erwinia phage vB_EhrS_49 OX=2283026 GN=MZUP3_590 PE=4 SV=1
MM1 pKa = 7.48KK2 pKa = 10.11PIDD5 pKa = 3.8HH6 pKa = 6.68YY7 pKa = 11.28RR8 pKa = 11.84LAMYY12 pKa = 9.65LQTRR16 pKa = 11.84KK17 pKa = 9.33QLEE20 pKa = 3.96NRR22 pKa = 11.84LAEE25 pKa = 4.23VNSKK29 pKa = 9.94IEE31 pKa = 3.88RR32 pKa = 11.84VQYY35 pKa = 9.35QSRR38 pKa = 11.84SRR40 pKa = 11.84KK41 pKa = 9.25PLGQRR46 pKa = 11.84ILNWWFAA53 pKa = 3.71

Molecular weight: 6.56 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

80

0

80

14242

38

1051

178.0

19.74

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.385 ± 0.667

1.32 ± 0.196

6.347 ± 0.229

6.326 ± 0.427

3.419 ± 0.21

6.916 ± 0.337

1.51 ± 0.193

5.744 ± 0.232

6.109 ± 0.407

7.38 ± 0.24

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.64 ± 0.164

4.353 ± 0.263

3.539 ± 0.243

4.585 ± 0.327

5.666 ± 0.333

6.649 ± 0.336

6.361 ± 0.41

6.109 ± 0.308

1.608 ± 0.134

3.033 ± 0.209
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski