endosymbiont of Euscepes postfasciatus

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; unclassified Gammaproteobacteria

Average proteome isoelectric point is 8.7

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 210 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2Z5TN74|A0A2Z5TN74_9GAMM Peptidase_M23 domain-containing protein OS=endosymbiont of Euscepes postfasciatus OX=650377 GN=yebA PE=4 SV=1
MM1 pKa = 7.41SNKK4 pKa = 10.17SINKK8 pKa = 9.35VILIGYY14 pKa = 8.39LGQDD18 pKa = 3.55PDD20 pKa = 4.46SKK22 pKa = 10.48YY23 pKa = 9.29TNNGNMVTTINLATSNKK40 pKa = 9.01WKK42 pKa = 10.16DD43 pKa = 3.51KK44 pKa = 10.82KK45 pKa = 11.02SGEE48 pKa = 4.23IKK50 pKa = 10.89NKK52 pKa = 8.41TEE54 pKa = 3.15WHH56 pKa = 6.41RR57 pKa = 11.84IVFFGKK63 pKa = 9.61IAEE66 pKa = 4.25ICSEE70 pKa = 4.03YY71 pKa = 10.63LKK73 pKa = 10.54KK74 pKa = 10.8GSYY77 pKa = 9.86IYY79 pKa = 10.97VEE81 pKa = 4.41GQLQTRR87 pKa = 11.84KK88 pKa = 8.7WKK90 pKa = 9.54NQEE93 pKa = 4.62GIDD96 pKa = 3.87KK97 pKa = 10.18YY98 pKa = 8.68ITEE101 pKa = 5.12IIVSVNGIMKK111 pKa = 10.0ILNNIKK117 pKa = 10.27QNWNEE122 pKa = 3.73SSFIKK127 pKa = 10.78NNSEE131 pKa = 3.52IKK133 pKa = 10.99NNIDD137 pKa = 3.16EE138 pKa = 5.71DD139 pKa = 4.49DD140 pKa = 4.59DD141 pKa = 5.25NEE143 pKa = 4.53EE144 pKa = 4.95NDD146 pKa = 5.39DD147 pKa = 4.35DD148 pKa = 5.6DD149 pKa = 6.89FNIKK153 pKa = 10.81DD154 pKa = 3.85NFEE157 pKa = 5.17DD158 pKa = 5.01DD159 pKa = 5.27DD160 pKa = 4.42ISFF163 pKa = 4.07

Molecular weight: 18.96 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2Z5T2W6|A0A2Z5T2W6_9GAMM Glycine--tRNA ligase beta subunit OS=endosymbiont of Euscepes postfasciatus OX=650377 GN=glyS PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.59RR3 pKa = 11.84TFQPSKK9 pKa = 9.52IKK11 pKa = 10.5RR12 pKa = 11.84IRR14 pKa = 11.84KK15 pKa = 8.62LGFRR19 pKa = 11.84SRR21 pKa = 11.84MSTKK25 pKa = 10.02SGRR28 pKa = 11.84KK29 pKa = 8.77IISNRR34 pKa = 11.84RR35 pKa = 11.84KK36 pKa = 9.8KK37 pKa = 10.69GRR39 pKa = 11.84LHH41 pKa = 7.51LIPKK45 pKa = 8.28VIKK48 pKa = 8.76YY49 pKa = 9.95HH50 pKa = 6.35KK51 pKa = 10.34

Molecular weight: 6.19 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

210

0

210

66724

38

1341

317.7

37.27

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

1.631 ± 0.102

1.064 ± 0.047

4.184 ± 0.115

4.153 ± 0.14

5.891 ± 0.157

3.978 ± 0.142

1.114 ± 0.044

16.844 ± 0.208

13.619 ± 0.211

8.956 ± 0.123

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.503 ± 0.056

13.234 ± 0.274

1.9 ± 0.061

1.295 ± 0.057

2.256 ± 0.099

6.386 ± 0.151

2.924 ± 0.077

2.854 ± 0.102

0.51 ± 0.051

5.706 ± 0.129
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski