Gordonia phage NosilaM

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.11

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 92 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A410TE65|A0A410TE65_9CAUD Uncharacterized protein OS=Gordonia phage NosilaM OX=2507863 GN=70 PE=4 SV=1
MM1 pKa = 7.17KK2 pKa = 9.87TRR4 pKa = 11.84IAIFLLAAVVFVFGPIAAALTGEE27 pKa = 4.43VEE29 pKa = 4.18AAPVRR34 pKa = 11.84PCTMVTATGTAPCPPPIVSTDD55 pKa = 2.74GSTIGGDD62 pKa = 3.33ANTAPVNMGEE72 pKa = 4.4LPRR75 pKa = 11.84TGPDD79 pKa = 3.21YY80 pKa = 10.7TYY82 pKa = 10.29EE83 pKa = 4.2APTYY87 pKa = 10.59FDD89 pKa = 5.54DD90 pKa = 4.42EE91 pKa = 4.51TDD93 pKa = 3.34EE94 pKa = 5.08GPAEE98 pKa = 4.02EE99 pKa = 5.23PGDD102 pKa = 4.57GEE104 pKa = 6.05DD105 pKa = 4.23EE106 pKa = 4.42DD107 pKa = 5.48ASS109 pKa = 3.78

Molecular weight: 11.19 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A410TE20|A0A410TE20_9CAUD Uncharacterized protein OS=Gordonia phage NosilaM OX=2507863 GN=25 PE=4 SV=1
MM1 pKa = 7.71PMDD4 pKa = 4.92APQAGAKK11 pKa = 9.1PKK13 pKa = 8.79WRR15 pKa = 11.84FRR17 pKa = 11.84KK18 pKa = 9.21EE19 pKa = 3.41VRR21 pKa = 11.84QFYY24 pKa = 10.48QRR26 pKa = 11.84LEE28 pKa = 3.98AAGGEE33 pKa = 3.78IRR35 pKa = 11.84YY36 pKa = 8.92PRR38 pKa = 11.84GNGHH42 pKa = 7.0PKK44 pKa = 10.35LFYY47 pKa = 10.08EE48 pKa = 4.74GQFIATLGMTTKK60 pKa = 10.46DD61 pKa = 3.34RR62 pKa = 11.84HH63 pKa = 5.05WDD65 pKa = 3.51RR66 pKa = 11.84NVISACRR73 pKa = 11.84RR74 pKa = 11.84AGMTII79 pKa = 3.32

Molecular weight: 9.17 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

92

0

92

21332

47

1808

231.9

25.51

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.323 ± 0.401

0.703 ± 0.104

7.088 ± 0.253

6.769 ± 0.344

3.178 ± 0.147

8.822 ± 0.392

1.903 ± 0.168

4.96 ± 0.205

4.008 ± 0.22

7.454 ± 0.256

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.236 ± 0.114

3.249 ± 0.165

5.56 ± 0.182

3.221 ± 0.133

7.43 ± 0.369

5.1 ± 0.205

6.596 ± 0.215

6.924 ± 0.208

1.941 ± 0.103

2.536 ± 0.182
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski