Vibrio phage martha 12B12

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; unclassified Myoviridae

Average proteome isoelectric point is 6.16

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 51 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|M4MHE6|M4MHE6_9CAUD Phage tail tape measure protein OS=Vibrio phage martha 12B12 OX=573175 GN=VPCG_00008 PE=4 SV=1
MM1 pKa = 7.7PFNVPTLRR9 pKa = 11.84QLIEE13 pKa = 4.24SGLIDD18 pKa = 4.3IEE20 pKa = 4.62ASLDD24 pKa = 3.4TLLPKK29 pKa = 10.71FGIEE33 pKa = 3.78QALNASVSGSIRR45 pKa = 11.84DD46 pKa = 3.63LYY48 pKa = 10.99DD49 pKa = 3.13YY50 pKa = 7.57QTWIVRR56 pKa = 11.84QIIPSSEE63 pKa = 4.4SEE65 pKa = 4.21DD66 pKa = 3.29QTIIDD71 pKa = 3.64TARR74 pKa = 11.84YY75 pKa = 9.22EE76 pKa = 4.43GVLQKK81 pKa = 10.73LASNAFGPVTFTGDD95 pKa = 3.07VPIPVDD101 pKa = 3.41TVMTHH106 pKa = 5.67SDD108 pKa = 2.76GRR110 pKa = 11.84LYY112 pKa = 10.71RR113 pKa = 11.84VTLSNAPSGGTVVVQVEE130 pKa = 4.11AEE132 pKa = 4.1EE133 pKa = 4.58AGVAGNLDD141 pKa = 3.46SDD143 pKa = 3.82QTLTLVSTVPCIQPNGITGEE163 pKa = 3.96ISGGADD169 pKa = 3.26IEE171 pKa = 4.5PVAQVLEE178 pKa = 3.95RR179 pKa = 11.84LLFRR183 pKa = 11.84KK184 pKa = 9.86RR185 pKa = 11.84NPPMGGALHH194 pKa = 7.57DD195 pKa = 3.91YY196 pKa = 9.56VAWCRR201 pKa = 11.84EE202 pKa = 3.84VSGVTRR208 pKa = 11.84AWCIDD213 pKa = 4.44LYY215 pKa = 10.94QGPATVGYY223 pKa = 10.59AFVYY227 pKa = 10.06DD228 pKa = 4.15EE229 pKa = 4.92RR230 pKa = 11.84ADD232 pKa = 3.98IIPTYY237 pKa = 9.12TDD239 pKa = 3.05QVAMTDD245 pKa = 3.4YY246 pKa = 10.55IYY248 pKa = 10.64RR249 pKa = 11.84HH250 pKa = 6.94PDD252 pKa = 3.09PATGTDD258 pKa = 2.9VGRR261 pKa = 11.84PGGIEE266 pKa = 3.67AVYY269 pKa = 10.39IPLQLKK275 pKa = 8.03EE276 pKa = 4.21TDD278 pKa = 3.33LGITISPDD286 pKa = 3.14NTDD289 pKa = 3.05LRR291 pKa = 11.84QSVKK295 pKa = 10.12TSIEE299 pKa = 4.25GYY301 pKa = 10.12IKK303 pKa = 9.75TLSPGSTLQLSSVRR317 pKa = 11.84TAIGSTPGVSDD328 pKa = 3.88YY329 pKa = 11.76VLDD332 pKa = 4.46LNADD336 pKa = 3.67VPALSSEE343 pKa = 3.96LHH345 pKa = 5.89ALGVITWGTMM355 pKa = 2.9

Molecular weight: 38.19 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|M4M9R2|M4M9R2_9CAUD Transposase OS=Vibrio phage martha 12B12 OX=573175 GN=VPCG_00047 PE=4 SV=1
MM1 pKa = 7.48LLISTRR7 pKa = 11.84YY8 pKa = 10.26GEE10 pKa = 4.48INVSRR15 pKa = 11.84HH16 pKa = 4.92AIEE19 pKa = 4.83RR20 pKa = 11.84SRR22 pKa = 11.84QRR24 pKa = 11.84TGRR27 pKa = 11.84SLPQLVEE34 pKa = 4.2AVAKK38 pKa = 10.66ANRR41 pKa = 11.84PSKK44 pKa = 10.52NRR46 pKa = 11.84LRR48 pKa = 11.84RR49 pKa = 11.84IMKK52 pKa = 10.09CEE54 pKa = 4.21SGWQPKK60 pKa = 10.11RR61 pKa = 11.84ILEE64 pKa = 4.08SDD66 pKa = 3.44CAYY69 pKa = 10.95FLIRR73 pKa = 11.84NNNIVTVYY81 pKa = 10.74DD82 pKa = 3.52KK83 pKa = 11.13RR84 pKa = 11.84KK85 pKa = 9.59EE86 pKa = 4.51GYY88 pKa = 9.25SHH90 pKa = 7.3AA91 pKa = 4.63

Molecular weight: 10.65 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

51

0

51

10493

50

663

205.7

22.91

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.625 ± 0.344

0.915 ± 0.133

6.109 ± 0.264

7.405 ± 0.378

3.336 ± 0.216

7.062 ± 0.317

1.496 ± 0.185

5.346 ± 0.22

6.223 ± 0.405

8.596 ± 0.273

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.792 ± 0.168

4.632 ± 0.203

4.088 ± 0.199

4.393 ± 0.225

5.251 ± 0.246

6.643 ± 0.284

5.661 ± 0.302

6.919 ± 0.296

1.658 ± 0.137

2.85 ± 0.225
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski