Amino acid dipepetide frequency for Mycobacterium phage Spoonbill

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
13.28AlaAla: 13.28 ± 1.714
1.187AlaCys: 1.187 ± 0.308
7.177AlaAsp: 7.177 ± 0.645
7.233AlaGlu: 7.233 ± 0.832
2.712AlaPhe: 2.712 ± 0.428
10.115AlaGly: 10.115 ± 1.211
2.543AlaHis: 2.543 ± 0.436
4.351AlaIle: 4.351 ± 0.575
4.238AlaLys: 4.238 ± 0.455
7.572AlaLeu: 7.572 ± 0.847
2.712AlaMet: 2.712 ± 0.416
3.052AlaAsn: 3.052 ± 0.507
4.634AlaPro: 4.634 ± 0.506
3.278AlaGln: 3.278 ± 0.416
7.798AlaArg: 7.798 ± 0.854
5.538AlaSer: 5.538 ± 0.622
6.499AlaThr: 6.499 ± 0.58
6.329AlaVal: 6.329 ± 0.538
2.147AlaTrp: 2.147 ± 0.403
2.373AlaTyr: 2.373 ± 0.373
0.0AlaXaa: 0.0 ± 0.0
Cys
0.904CysAla: 0.904 ± 0.269
0.0CysCys: 0.0 ± 0.0
1.413CysAsp: 1.413 ± 0.337
0.622CysGlu: 0.622 ± 0.172
0.226CysPhe: 0.226 ± 0.099
1.639CysGly: 1.639 ± 0.359
0.283CysHis: 0.283 ± 0.125
0.339CysIle: 0.339 ± 0.171
0.622CysLys: 0.622 ± 0.209
0.678CysLeu: 0.678 ± 0.203
0.113CysMet: 0.113 ± 0.071
0.452CysAsn: 0.452 ± 0.164
1.074CysPro: 1.074 ± 0.291
0.396CysGln: 0.396 ± 0.152
0.735CysArg: 0.735 ± 0.227
0.678CysSer: 0.678 ± 0.216
0.735CysThr: 0.735 ± 0.235
0.678CysVal: 0.678 ± 0.161
0.339CysTrp: 0.339 ± 0.13
0.17CysTyr: 0.17 ± 0.096
0.0CysXaa: 0.0 ± 0.0
Asp
7.29AspAla: 7.29 ± 0.741
0.735AspCys: 0.735 ± 0.166
4.464AspAsp: 4.464 ± 0.586
3.447AspGlu: 3.447 ± 0.386
1.921AspPhe: 1.921 ± 0.302
6.329AspGly: 6.329 ± 0.58
1.187AspHis: 1.187 ± 0.304
2.091AspIle: 2.091 ± 0.372
1.921AspLys: 1.921 ± 0.289
5.764AspLeu: 5.764 ± 0.521
1.243AspMet: 1.243 ± 0.305
2.091AspAsn: 2.091 ± 0.414
4.577AspPro: 4.577 ± 0.494
2.712AspGln: 2.712 ± 0.322
5.142AspArg: 5.142 ± 0.636
3.56AspSer: 3.56 ± 0.485
3.673AspThr: 3.673 ± 0.56
4.577AspVal: 4.577 ± 0.645
1.639AspTrp: 1.639 ± 0.263
1.978AspTyr: 1.978 ± 0.346
0.0AspXaa: 0.0 ± 0.0
Glu
6.329GluAla: 6.329 ± 0.769
0.678GluCys: 0.678 ± 0.215
3.221GluAsp: 3.221 ± 0.385
3.221GluGlu: 3.221 ± 0.553
2.147GluPhe: 2.147 ± 0.371
3.334GluGly: 3.334 ± 0.406
2.034GluHis: 2.034 ± 0.454
2.317GluIle: 2.317 ± 0.378
2.204GluLys: 2.204 ± 0.301
5.594GluLeu: 5.594 ± 0.702
1.356GluMet: 1.356 ± 0.322
1.921GluAsn: 1.921 ± 0.346
3.108GluPro: 3.108 ± 0.495
2.599GluGln: 2.599 ± 0.405
5.086GluArg: 5.086 ± 0.631
3.052GluSer: 3.052 ± 0.46
4.182GluThr: 4.182 ± 0.57
4.238GluVal: 4.238 ± 0.612
1.526GluTrp: 1.526 ± 0.276
1.865GluTyr: 1.865 ± 0.354
0.0GluXaa: 0.0 ± 0.0
Phe
2.769PheAla: 2.769 ± 0.421
0.283PheCys: 0.283 ± 0.129
2.486PheAsp: 2.486 ± 0.481
1.695PheGlu: 1.695 ± 0.306
0.848PhePhe: 0.848 ± 0.217
2.882PheGly: 2.882 ± 0.705
0.283PheHis: 0.283 ± 0.126
1.356PheIle: 1.356 ± 0.341
0.961PheLys: 0.961 ± 0.237
1.808PheLeu: 1.808 ± 0.29
0.565PheMet: 0.565 ± 0.178
1.356PheAsn: 1.356 ± 0.378
1.526PhePro: 1.526 ± 0.292
1.3PheGln: 1.3 ± 0.332
1.695PheArg: 1.695 ± 0.283
1.3PheSer: 1.3 ± 0.29
2.26PheThr: 2.26 ± 0.381
2.034PheVal: 2.034 ± 0.241
0.339PheTrp: 0.339 ± 0.124
0.904PheTyr: 0.904 ± 0.265
0.0PheXaa: 0.0 ± 0.0
Gly
9.494GlyAla: 9.494 ± 1.077
1.074GlyCys: 1.074 ± 0.262
5.708GlyAsp: 5.708 ± 0.521
4.464GlyGlu: 4.464 ± 0.547
2.373GlyPhe: 2.373 ± 0.389
10.963GlyGly: 10.963 ± 2.184
1.695GlyHis: 1.695 ± 0.31
3.786GlyIle: 3.786 ± 0.706
2.599GlyLys: 2.599 ± 0.364
5.594GlyLeu: 5.594 ± 0.54
2.599GlyMet: 2.599 ± 0.548
2.882GlyAsn: 2.882 ± 0.394
4.182GlyPro: 4.182 ± 0.593
2.034GlyGln: 2.034 ± 0.56
5.425GlyArg: 5.425 ± 0.678
6.499GlySer: 6.499 ± 1.049
6.499GlyThr: 6.499 ± 0.838
5.934GlyVal: 5.934 ± 0.588
2.486GlyTrp: 2.486 ± 0.368
2.034GlyTyr: 2.034 ± 0.426
0.0GlyXaa: 0.0 ± 0.0
His
2.091HisAla: 2.091 ± 0.316
0.283HisCys: 0.283 ± 0.165
1.074HisAsp: 1.074 ± 0.194
1.017HisGlu: 1.017 ± 0.245
0.509HisPhe: 0.509 ± 0.145
1.469HisGly: 1.469 ± 0.294
0.735HisHis: 0.735 ± 0.261
1.469HisIle: 1.469 ± 0.305
0.904HisLys: 0.904 ± 0.238
1.469HisLeu: 1.469 ± 0.32
0.452HisMet: 0.452 ± 0.139
0.848HisAsn: 0.848 ± 0.214
1.356HisPro: 1.356 ± 0.266
0.848HisGln: 0.848 ± 0.194
2.091HisArg: 2.091 ± 0.375
0.904HisSer: 0.904 ± 0.199
1.582HisThr: 1.582 ± 0.343
1.865HisVal: 1.865 ± 0.419
0.339HisTrp: 0.339 ± 0.138
0.848HisTyr: 0.848 ± 0.211
0.0HisXaa: 0.0 ± 0.0
Ile
4.86IleAla: 4.86 ± 0.506
0.678IleCys: 0.678 ± 0.24
4.238IleAsp: 4.238 ± 0.532
3.391IleGlu: 3.391 ± 0.429
0.678IlePhe: 0.678 ± 0.214
3.56IleGly: 3.56 ± 0.527
1.413IleHis: 1.413 ± 0.351
1.582IleIle: 1.582 ± 0.309
0.904IleLys: 0.904 ± 0.219
2.091IleLeu: 2.091 ± 0.373
0.339IleMet: 0.339 ± 0.121
1.752IleAsn: 1.752 ± 0.314
3.447IlePro: 3.447 ± 0.368
1.469IleGln: 1.469 ± 0.299
2.486IleArg: 2.486 ± 0.507
2.486IleSer: 2.486 ± 0.455
3.956IleThr: 3.956 ± 0.602
3.278IleVal: 3.278 ± 0.359
0.904IleTrp: 0.904 ± 0.224
0.678IleTyr: 0.678 ± 0.177
0.0IleXaa: 0.0 ± 0.0
Lys
3.956LysAla: 3.956 ± 0.67
0.452LysCys: 0.452 ± 0.168
1.695LysAsp: 1.695 ± 0.313
1.187LysGlu: 1.187 ± 0.281
1.356LysPhe: 1.356 ± 0.219
2.712LysGly: 2.712 ± 0.405
1.017LysHis: 1.017 ± 0.274
0.904LysIle: 0.904 ± 0.228
1.582LysLys: 1.582 ± 0.476
2.543LysLeu: 2.543 ± 0.486
0.622LysMet: 0.622 ± 0.185
1.074LysAsn: 1.074 ± 0.233
2.825LysPro: 2.825 ± 0.53
1.808LysGln: 1.808 ± 0.317
2.204LysArg: 2.204 ± 0.412
2.204LysSer: 2.204 ± 0.298
1.921LysThr: 1.921 ± 0.383
2.373LysVal: 2.373 ± 0.38
1.074LysTrp: 1.074 ± 0.331
0.565LysTyr: 0.565 ± 0.177
0.0LysXaa: 0.0 ± 0.0
Leu
7.572LeuAla: 7.572 ± 0.72
0.848LeuCys: 0.848 ± 0.193
4.012LeuAsp: 4.012 ± 0.504
4.521LeuGlu: 4.521 ± 0.609
2.147LeuPhe: 2.147 ± 0.299
4.69LeuGly: 4.69 ± 0.498
1.017LeuHis: 1.017 ± 0.229
2.882LeuIle: 2.882 ± 0.406
2.43LeuLys: 2.43 ± 0.462
4.86LeuLeu: 4.86 ± 0.57
2.034LeuMet: 2.034 ± 0.345
2.825LeuAsn: 2.825 ± 0.423
4.973LeuPro: 4.973 ± 0.619
2.769LeuGln: 2.769 ± 0.451
4.973LeuArg: 4.973 ± 0.609
5.086LeuSer: 5.086 ± 0.525
5.481LeuThr: 5.481 ± 0.54
4.916LeuVal: 4.916 ± 0.452
1.13LeuTrp: 1.13 ± 0.284
2.147LeuTyr: 2.147 ± 0.389
0.0LeuXaa: 0.0 ± 0.0
Met
2.147MetAla: 2.147 ± 0.322
0.17MetCys: 0.17 ± 0.135
1.3MetAsp: 1.3 ± 0.284
1.017MetGlu: 1.017 ± 0.203
0.622MetPhe: 0.622 ± 0.2
1.526MetGly: 1.526 ± 0.27
0.113MetHis: 0.113 ± 0.083
0.848MetIle: 0.848 ± 0.211
0.509MetLys: 0.509 ± 0.16
1.752MetLeu: 1.752 ± 0.287
0.452MetMet: 0.452 ± 0.205
1.074MetAsn: 1.074 ± 0.24
1.3MetPro: 1.3 ± 0.267
0.509MetGln: 0.509 ± 0.149
1.921MetArg: 1.921 ± 0.289
2.769MetSer: 2.769 ± 0.409
2.034MetThr: 2.034 ± 0.301
1.469MetVal: 1.469 ± 0.29
0.339MetTrp: 0.339 ± 0.117
0.452MetTyr: 0.452 ± 0.174
0.0MetXaa: 0.0 ± 0.0
Asn
3.56AsnAla: 3.56 ± 0.423
0.396AsnCys: 0.396 ± 0.128
1.808AsnAsp: 1.808 ± 0.3
1.865AsnGlu: 1.865 ± 0.403
0.791AsnPhe: 0.791 ± 0.25
4.238AsnGly: 4.238 ± 0.53
0.904AsnHis: 0.904 ± 0.207
1.582AsnIle: 1.582 ± 0.467
1.187AsnLys: 1.187 ± 0.274
2.543AsnLeu: 2.543 ± 0.417
0.848AsnMet: 0.848 ± 0.168
1.865AsnAsn: 1.865 ± 0.358
2.825AsnPro: 2.825 ± 0.339
1.074AsnGln: 1.074 ± 0.385
1.865AsnArg: 1.865 ± 0.319
1.582AsnSer: 1.582 ± 0.328
2.034AsnThr: 2.034 ± 0.339
2.26AsnVal: 2.26 ± 0.344
0.678AsnTrp: 0.678 ± 0.229
0.735AsnTyr: 0.735 ± 0.172
0.0AsnXaa: 0.0 ± 0.0
Pro
5.538ProAla: 5.538 ± 0.654
0.735ProCys: 0.735 ± 0.193
4.125ProAsp: 4.125 ± 0.444
4.408ProGlu: 4.408 ± 0.545
1.865ProPhe: 1.865 ± 0.339
6.612ProGly: 6.612 ± 0.7
1.526ProHis: 1.526 ± 0.279
2.034ProIle: 2.034 ± 0.325
2.486ProLys: 2.486 ± 0.382
4.803ProLeu: 4.803 ± 0.527
1.13ProMet: 1.13 ± 0.241
1.921ProAsn: 1.921 ± 0.258
4.521ProPro: 4.521 ± 0.557
2.486ProGln: 2.486 ± 0.479
3.334ProArg: 3.334 ± 0.485
3.052ProSer: 3.052 ± 0.469
4.012ProThr: 4.012 ± 0.513
4.182ProVal: 4.182 ± 0.44
1.243ProTrp: 1.243 ± 0.257
1.469ProTyr: 1.469 ± 0.262
0.0ProXaa: 0.0 ± 0.0
Gln
4.408GlnAla: 4.408 ± 0.671
0.339GlnCys: 0.339 ± 0.188
1.469GlnAsp: 1.469 ± 0.277
1.695GlnGlu: 1.695 ± 0.308
1.187GlnPhe: 1.187 ± 0.294
2.656GlnGly: 2.656 ± 0.482
1.017GlnHis: 1.017 ± 0.218
1.808GlnIle: 1.808 ± 0.34
1.243GlnLys: 1.243 ± 0.215
2.882GlnLeu: 2.882 ± 0.365
0.622GlnMet: 0.622 ± 0.202
1.13GlnAsn: 1.13 ± 0.313
2.882GlnPro: 2.882 ± 0.414
1.243GlnGln: 1.243 ± 0.317
2.543GlnArg: 2.543 ± 0.427
2.204GlnSer: 2.204 ± 0.345
1.469GlnThr: 1.469 ± 0.245
2.543GlnVal: 2.543 ± 0.381
0.735GlnTrp: 0.735 ± 0.221
0.961GlnTyr: 0.961 ± 0.247
0.0GlnXaa: 0.0 ± 0.0
Arg
6.838ArgAla: 6.838 ± 0.697
0.961ArgCys: 0.961 ± 0.258
4.916ArgAsp: 4.916 ± 0.64
5.029ArgGlu: 5.029 ± 0.747
2.204ArgPhe: 2.204 ± 0.403
4.125ArgGly: 4.125 ± 0.437
1.243ArgHis: 1.243 ± 0.275
3.956ArgIle: 3.956 ± 0.585
2.317ArgLys: 2.317 ± 0.381
4.973ArgLeu: 4.973 ± 0.548
2.204ArgMet: 2.204 ± 0.354
2.486ArgAsn: 2.486 ± 0.342
3.786ArgPro: 3.786 ± 0.446
1.695ArgGln: 1.695 ± 0.344
5.425ArgArg: 5.425 ± 0.698
3.73ArgSer: 3.73 ± 0.453
3.673ArgThr: 3.673 ± 0.5
5.029ArgVal: 5.029 ± 0.668
1.921ArgTrp: 1.921 ± 0.356
2.034ArgTyr: 2.034 ± 0.269
0.0ArgXaa: 0.0 ± 0.0
Ser
6.047SerAla: 6.047 ± 0.823
0.622SerCys: 0.622 ± 0.206
4.86SerAsp: 4.86 ± 0.492
3.504SerGlu: 3.504 ± 0.566
1.582SerPhe: 1.582 ± 0.272
6.499SerGly: 6.499 ± 1.08
0.961SerHis: 0.961 ± 0.245
2.825SerIle: 2.825 ± 0.477
1.978SerLys: 1.978 ± 0.346
3.617SerLeu: 3.617 ± 0.378
1.695SerMet: 1.695 ± 0.335
2.204SerAsn: 2.204 ± 0.402
3.504SerPro: 3.504 ± 0.344
1.752SerGln: 1.752 ± 0.28
3.334SerArg: 3.334 ± 0.321
3.73SerSer: 3.73 ± 0.65
3.56SerThr: 3.56 ± 0.505
4.803SerVal: 4.803 ± 0.668
1.3SerTrp: 1.3 ± 0.258
1.3SerTyr: 1.3 ± 0.228
0.0SerXaa: 0.0 ± 0.0
Thr
6.386ThrAla: 6.386 ± 0.755
0.622ThrCys: 0.622 ± 0.217
4.069ThrAsp: 4.069 ± 0.49
3.899ThrGlu: 3.899 ± 0.491
1.921ThrPhe: 1.921 ± 0.335
6.612ThrGly: 6.612 ± 0.662
1.639ThrHis: 1.639 ± 0.36
4.069ThrIle: 4.069 ± 0.512
1.752ThrLys: 1.752 ± 0.383
3.899ThrLeu: 3.899 ± 0.446
1.356ThrMet: 1.356 ± 0.266
2.091ThrAsn: 2.091 ± 0.396
4.86ThrPro: 4.86 ± 0.594
2.43ThrGln: 2.43 ± 0.348
3.56ThrArg: 3.56 ± 0.468
3.899ThrSer: 3.899 ± 0.57
4.238ThrThr: 4.238 ± 0.582
5.934ThrVal: 5.934 ± 0.641
0.961ThrTrp: 0.961 ± 0.235
1.356ThrTyr: 1.356 ± 0.298
0.0ThrXaa: 0.0 ± 0.0
Val
7.346ValAla: 7.346 ± 0.633
1.187ValCys: 1.187 ± 0.256
5.029ValAsp: 5.029 ± 0.464
4.916ValGlu: 4.916 ± 0.683
2.147ValPhe: 2.147 ± 0.351
5.312ValGly: 5.312 ± 0.665
1.639ValHis: 1.639 ± 0.245
3.673ValIle: 3.673 ± 0.474
2.939ValLys: 2.939 ± 0.447
5.425ValLeu: 5.425 ± 0.638
0.904ValMet: 0.904 ± 0.164
2.091ValAsn: 2.091 ± 0.34
3.673ValPro: 3.673 ± 0.406
2.882ValGln: 2.882 ± 0.294
4.521ValArg: 4.521 ± 0.652
5.142ValSer: 5.142 ± 0.626
4.408ValThr: 4.408 ± 0.549
7.007ValVal: 7.007 ± 0.739
1.978ValTrp: 1.978 ± 0.362
1.413ValTyr: 1.413 ± 0.269
0.0ValXaa: 0.0 ± 0.0
Trp
1.808TrpAla: 1.808 ± 0.26
0.283TrpCys: 0.283 ± 0.119
1.469TrpAsp: 1.469 ± 0.251
0.961TrpGlu: 0.961 ± 0.299
0.678TrpPhe: 0.678 ± 0.228
1.13TrpGly: 1.13 ± 0.251
0.565TrpHis: 0.565 ± 0.167
1.074TrpIle: 1.074 ± 0.192
0.735TrpLys: 0.735 ± 0.187
1.695TrpLeu: 1.695 ± 0.397
0.904TrpMet: 0.904 ± 0.222
0.622TrpAsn: 0.622 ± 0.207
0.961TrpPro: 0.961 ± 0.26
1.187TrpGln: 1.187 ± 0.281
2.43TrpArg: 2.43 ± 0.424
1.469TrpSer: 1.469 ± 0.284
1.582TrpThr: 1.582 ± 0.305
1.752TrpVal: 1.752 ± 0.448
1.074TrpTrp: 1.074 ± 0.251
0.452TrpTyr: 0.452 ± 0.177
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.147TyrAla: 2.147 ± 0.416
0.565TyrCys: 0.565 ± 0.215
1.808TyrAsp: 1.808 ± 0.393
1.978TyrGlu: 1.978 ± 0.31
0.735TyrPhe: 0.735 ± 0.205
1.921TyrGly: 1.921 ± 0.308
0.339TyrHis: 0.339 ± 0.13
1.243TyrIle: 1.243 ± 0.271
0.565TyrLys: 0.565 ± 0.195
1.752TyrLeu: 1.752 ± 0.288
0.113TyrMet: 0.113 ± 0.073
0.904TyrAsn: 0.904 ± 0.217
1.469TyrPro: 1.469 ± 0.303
0.565TyrGln: 0.565 ± 0.21
1.978TyrArg: 1.978 ± 0.378
0.791TyrSer: 0.791 ± 0.23
1.752TyrThr: 1.752 ± 0.336
2.43TyrVal: 2.43 ± 0.333
0.678TyrTrp: 0.678 ± 0.159
0.622TyrTyr: 0.622 ± 0.187
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 103 proteins (17697 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski