Talaromyces amestolkiae

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; Eurotiomycetes; Eurotiomycetidae; Eurotiales; Trichocomaceae; Talaromyces; Talaromyces sect. Talaromyces

Average proteome isoelectric point is 6.16

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 10407 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A364KSC5|A0A364KSC5_9EURO INCENP_ARK-bind domain-containing protein OS=Talaromyces amestolkiae OX=1196081 GN=BHQ10_002457 PE=3 SV=1
MM1 pKa = 7.85PSLAQVSLFLSLTISGLVQAAPAPAPAPAPAPTAPPKK38 pKa = 10.3LSEE41 pKa = 4.2RR42 pKa = 11.84ASSCTFSGSKK52 pKa = 9.97GASSASKK59 pKa = 10.7SKK61 pKa = 9.53TSCSTIVLSDD71 pKa = 3.44VAVPSGTTLDD81 pKa = 3.9LTDD84 pKa = 5.36LNDD87 pKa = 3.68GTSVIFEE94 pKa = 4.31GTTSFGYY101 pKa = 9.98EE102 pKa = 3.81EE103 pKa = 4.47WDD105 pKa = 3.72GPLISVSGTDD115 pKa = 3.12ITVTGAEE122 pKa = 4.02GHH124 pKa = 6.59VIDD127 pKa = 5.67GDD129 pKa = 4.04GSRR132 pKa = 11.84WWDD135 pKa = 3.42GEE137 pKa = 3.95GSNGGTTKK145 pKa = 10.62PKK147 pKa = 10.28FFYY150 pKa = 10.74AHH152 pKa = 7.7DD153 pKa = 4.24LTSSTISGLSLKK165 pKa = 10.27NSPVQTFSIDD175 pKa = 2.93GSTDD179 pKa = 3.11LTLSDD184 pKa = 3.63ITIDD188 pKa = 5.56DD189 pKa = 3.68SDD191 pKa = 5.41GDD193 pKa = 4.34DD194 pKa = 3.62GSAANTDD201 pKa = 3.33AFDD204 pKa = 3.59VGEE207 pKa = 4.22STGIIISGATVYY219 pKa = 10.98NQDD222 pKa = 3.5DD223 pKa = 4.0CLAINSGTNITFTGGYY239 pKa = 9.4CSGGHH244 pKa = 5.92GLSIGSVGGRR254 pKa = 11.84DD255 pKa = 3.95DD256 pKa = 3.81NTVSDD261 pKa = 3.97VTIEE265 pKa = 3.87SSTIIDD271 pKa = 3.47SANGVRR277 pKa = 11.84IKK279 pKa = 9.75TVYY282 pKa = 10.35DD283 pKa = 3.27ATGSVSGVTYY293 pKa = 10.99KK294 pKa = 10.92DD295 pKa = 3.0ITLSGITDD303 pKa = 3.45YY304 pKa = 11.69GIVIEE309 pKa = 4.22QDD311 pKa = 3.68YY312 pKa = 11.2EE313 pKa = 4.24NGSPTGTPTTGVPITDD329 pKa = 3.59LTIDD333 pKa = 3.64NVQGTVEE340 pKa = 3.96SDD342 pKa = 2.77ATEE345 pKa = 4.04IYY347 pKa = 9.99ILCGDD352 pKa = 4.84GSCSDD357 pKa = 3.67WTWTDD362 pKa = 3.21VSVTGGKK369 pKa = 10.03SSDD372 pKa = 3.37ACEE375 pKa = 4.05NAPDD379 pKa = 5.81DD380 pKa = 4.14ISCC383 pKa = 4.64

Molecular weight: 38.96 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A364LED8|A0A364LED8_9EURO ADP-ribose 1''-phosphate phosphatase OS=Talaromyces amestolkiae OX=1196081 GN=BHQ10_010169 PE=3 SV=1
MM1 pKa = 7.17PAKK4 pKa = 10.23GASTRR9 pKa = 11.84LNPVRR14 pKa = 11.84LQTIQHH20 pKa = 5.6LRR22 pKa = 11.84VRR24 pKa = 11.84RR25 pKa = 11.84PNQQQQNSCATIMSSMLNCWASAGQGNEE53 pKa = 3.91GCSGLEE59 pKa = 3.96EE60 pKa = 4.16SLKK63 pKa = 10.96ACMDD67 pKa = 3.3GRR69 pKa = 11.84RR70 pKa = 11.84DD71 pKa = 3.57STSSNNAVNYY81 pKa = 9.92HH82 pKa = 5.98LMRR85 pKa = 11.84MFPKK89 pKa = 10.4VSGPRR94 pKa = 11.84KK95 pKa = 9.27RR96 pKa = 11.84EE97 pKa = 3.63GRR99 pKa = 11.84LGG101 pKa = 3.44

Molecular weight: 11.16 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

10407

0

10407

5668149

28

7861

544.6

60.38

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.353 ± 0.017

1.115 ± 0.008

5.729 ± 0.017

6.191 ± 0.022

3.861 ± 0.013

6.652 ± 0.019

2.328 ± 0.01

5.468 ± 0.016

4.782 ± 0.021

9.021 ± 0.023

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.107 ± 0.009

3.984 ± 0.012

5.54 ± 0.022

4.026 ± 0.015

5.683 ± 0.018

8.293 ± 0.026

6.168 ± 0.018

6.215 ± 0.014

1.476 ± 0.009

2.982 ± 0.012
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski