Bacillus phage vB_BpsS-140

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.37

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 66 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3Q9JGU0|A0A3Q9JGU0_9CAUD Uncharacterized protein OS=Bacillus phage vB_BpsS-140 OX=2419621 GN=BpsS140_00027 PE=4 SV=1
MM1 pKa = 6.9GQTYY5 pKa = 10.05NVYY8 pKa = 10.75RR9 pKa = 11.84NGDD12 pKa = 4.45LIAEE16 pKa = 4.51GLTQKK21 pKa = 10.6SYY23 pKa = 11.58EE24 pKa = 4.13DD25 pKa = 3.55TGLDD29 pKa = 3.03SDD31 pKa = 3.74TTYY34 pKa = 10.5EE35 pKa = 4.23YY36 pKa = 11.17KK37 pKa = 9.56VTTVGEE43 pKa = 4.11WGGEE47 pKa = 4.13SEE49 pKa = 4.26PATLEE54 pKa = 4.03VTTEE58 pKa = 4.02SEE60 pKa = 4.26DD61 pKa = 5.08DD62 pKa = 3.89EE63 pKa = 5.7EE64 pKa = 5.09EE65 pKa = 4.26DD66 pKa = 4.08

Molecular weight: 7.36 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3Q9JM92|A0A3Q9JM92_9CAUD Chromosome segregation protein OS=Bacillus phage vB_BpsS-140 OX=2419621 GN=BpsS140_00032 PE=4 SV=1
MM1 pKa = 7.72TDD3 pKa = 3.63QEE5 pKa = 4.31FDD7 pKa = 3.67LLKK10 pKa = 10.62SYY12 pKa = 10.36QEE14 pKa = 4.1EE15 pKa = 3.96NRR17 pKa = 11.84QLKK20 pKa = 9.47IQLSRR25 pKa = 11.84KK26 pKa = 7.41NRR28 pKa = 11.84KK29 pKa = 8.38LRR31 pKa = 11.84EE32 pKa = 3.94YY33 pKa = 10.06KK34 pKa = 10.47HH35 pKa = 6.48VIRR38 pKa = 11.84KK39 pKa = 8.1QKK41 pKa = 10.55SAIEE45 pKa = 3.83RR46 pKa = 11.84LQEE49 pKa = 3.7NQRR52 pKa = 11.84PKK54 pKa = 10.48FRR56 pKa = 11.84NGKK59 pKa = 9.87RR60 pKa = 11.84GTIKK64 pKa = 10.78NGG66 pKa = 3.06

Molecular weight: 8.1 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

66

0

66

15454

43

2296

234.2

26.52

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.966 ± 0.253

0.556 ± 0.087

6.995 ± 0.271

7.137 ± 0.35

3.578 ± 0.211

6.406 ± 0.282

2.161 ± 0.229

8.05 ± 0.35

7.066 ± 0.408

7.746 ± 0.253

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.97 ± 0.212

5.384 ± 0.302

2.841 ± 0.293

3.714 ± 0.195

5.021 ± 0.296

7.144 ± 0.349

6.238 ± 0.363

6.186 ± 0.2

1.346 ± 0.219

3.494 ± 0.248
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski