Streptococcus satellite phage Javan327

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.53

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 15 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D5ZPQ8|A0A4D5ZPQ8_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan327 OX=2558639 GN=JavanS327_0013 PE=4 SV=1
MM1 pKa = 7.63IKK3 pKa = 10.78NNLANILKK11 pKa = 8.94TKK13 pKa = 10.03EE14 pKa = 3.73KK15 pKa = 8.17TAAWLSVKK23 pKa = 9.98TGITPAALSKK33 pKa = 10.44IVNNSSTKK41 pKa = 9.53IDD43 pKa = 4.19YY44 pKa = 7.92ITLNKK49 pKa = 9.57ICLALKK55 pKa = 10.24IEE57 pKa = 4.66PKK59 pKa = 10.76DD60 pKa = 3.65FFDD63 pKa = 4.66FLPYY67 pKa = 10.24DD68 pKa = 3.78YY69 pKa = 9.91EE70 pKa = 4.34VEE72 pKa = 4.26SIYY75 pKa = 10.95DD76 pKa = 3.49NKK78 pKa = 10.69DD79 pKa = 2.7IKK81 pKa = 11.24VSLKK85 pKa = 10.76SFEE88 pKa = 5.12LDD90 pKa = 3.23EE91 pKa = 5.18KK92 pKa = 10.71PNSSQIEE99 pKa = 4.19SLDD102 pKa = 4.04FYY104 pKa = 10.74QHH106 pKa = 5.61QADD109 pKa = 4.28NIDD112 pKa = 3.84IEE114 pKa = 4.63VSCDD118 pKa = 3.43FGLEE122 pKa = 4.13VNFMNTPMGDD132 pKa = 3.51NFSIWYY138 pKa = 8.7ISAYY142 pKa = 10.38FYY144 pKa = 11.42DD145 pKa = 4.01LDD147 pKa = 4.25YY148 pKa = 11.88NSFTVSIDD156 pKa = 3.19IDD158 pKa = 3.95DD159 pKa = 4.69DD160 pKa = 4.97SIGFLKK166 pKa = 10.41KK167 pKa = 10.69YY168 pKa = 9.07IAPYY172 pKa = 10.7DD173 pKa = 3.45KK174 pKa = 11.61GEE176 pKa = 3.92LFKK179 pKa = 10.63IIKK182 pKa = 10.01RR183 pKa = 11.84EE184 pKa = 4.02IISKK188 pKa = 9.36IQSNYY193 pKa = 9.65FDD195 pKa = 3.56WFEE198 pKa = 3.86NQYY201 pKa = 11.18GKK203 pKa = 10.67VSKK206 pKa = 10.79EE207 pKa = 3.51EE208 pKa = 3.88YY209 pKa = 10.95GFLKK213 pKa = 10.5SLNWWIGEE221 pKa = 3.88GTQNYY226 pKa = 8.75KK227 pKa = 10.78VNDD230 pKa = 3.65EE231 pKa = 5.48DD232 pKa = 3.7IQIMIDD238 pKa = 3.51IEE240 pKa = 4.4KK241 pKa = 10.51KK242 pKa = 10.41DD243 pKa = 3.35

Molecular weight: 28.35 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D5ZPY5|A0A4D5ZPY5_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan327 OX=2558639 GN=JavanS327_0009 PE=4 SV=1
MM1 pKa = 7.64FDD3 pKa = 4.35GFPEE7 pKa = 4.13RR8 pKa = 11.84PRR10 pKa = 11.84YY11 pKa = 8.12MKK13 pKa = 9.81RR14 pKa = 11.84DD15 pKa = 3.77RR16 pKa = 11.84YY17 pKa = 9.2HH18 pKa = 5.94KK19 pKa = 10.09HH20 pKa = 4.58YY21 pKa = 10.97KK22 pKa = 10.24KK23 pKa = 10.13FLKK26 pKa = 10.6YY27 pKa = 9.68IEE29 pKa = 4.58KK30 pKa = 10.6GDD32 pKa = 3.91RR33 pKa = 11.84FWLNGSRR40 pKa = 11.84LL41 pKa = 3.57

Molecular weight: 5.31 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

15

0

15

2486

41

388

165.7

19.18

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.994 ± 0.608

0.644 ± 0.181

6.034 ± 0.55

8.528 ± 0.765

3.982 ± 0.466

4.224 ± 0.304

1.73 ± 0.319

7.924 ± 0.687

9.533 ± 0.557

9.936 ± 0.784

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.414 ± 0.361

5.27 ± 0.554

2.333 ± 0.384

4.907 ± 0.462

4.505 ± 0.585

6.034 ± 0.394

6.074 ± 0.663

4.545 ± 0.36

0.845 ± 0.185

4.545 ± 0.33
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski