Paraburkholderia humisilvae

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; Burkholderiaceae; Paraburkholderia

Average proteome isoelectric point is 6.91

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 8698 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6J5EFD3|A0A6J5EFD3_9BURK Peripla_BP_6 domain-containing protein OS=Paraburkholderia humisilvae OX=627669 GN=LMG29542_05095 PE=4 SV=1
MM1 pKa = 6.89STVDD5 pKa = 3.53VFPSFNTDD13 pKa = 3.01MLSTPVDD20 pKa = 3.73SGSDD24 pKa = 3.67SPNLQQLEE32 pKa = 4.15QQYY35 pKa = 8.02MQLAQQFIQQGAGTLKK51 pKa = 8.85TQAAAGDD58 pKa = 3.85LASYY62 pKa = 7.53MTQDD66 pKa = 4.35NIGTLDD72 pKa = 3.76PNQLYY77 pKa = 10.79ALANNPPSGTPPGVSAAAAYY97 pKa = 7.73MLKK100 pKa = 10.44NSSAYY105 pKa = 9.27EE106 pKa = 3.81QIEE109 pKa = 4.26THH111 pKa = 6.87DD112 pKa = 3.68SSGADD117 pKa = 3.23GLSGVGNFQWASEE130 pKa = 4.31GGLSSASPFQMGSGSGFGGMSPFGGMPSMGGITAFGGMPSMGGMTSFGGMPSMGGMTSFGGMSSMGGMSSLGDD203 pKa = 3.32MSSFGDD209 pKa = 3.51MSSLFGSDD217 pKa = 3.23PLEE220 pKa = 4.26AQAAAGALASYY231 pKa = 9.47MSQNNIGALDD241 pKa = 3.99PNALYY246 pKa = 10.42QLANNPPPNMPPGVSEE262 pKa = 3.93AANYY266 pKa = 8.85MLEE269 pKa = 4.17NPDD272 pKa = 3.19IYY274 pKa = 10.98EE275 pKa = 4.02QIEE278 pKa = 4.19THH280 pKa = 6.68DD281 pKa = 3.55VAGADD286 pKa = 4.19GISGIGNFQWAAEE299 pKa = 4.38GGLMAGPAVSGSGGLPADD317 pKa = 4.49GGSGTDD323 pKa = 4.5DD324 pKa = 3.8PAEE327 pKa = 4.27NAPPANPSSSSGSTSGSSGGGTSSSTGGSTSGSKK361 pKa = 10.91GSDD364 pKa = 2.96STVPNISTSDD374 pKa = 3.58PAKK377 pKa = 10.71AIAEE381 pKa = 4.2DD382 pKa = 3.63LEE384 pKa = 4.14QRR386 pKa = 11.84YY387 pKa = 9.25GLTATQAAGVLGNLQQEE404 pKa = 4.65SGLQGDD410 pKa = 4.43VNQGGATGAPSSNDD424 pKa = 3.11ADD426 pKa = 4.53DD427 pKa = 5.25NGHH430 pKa = 5.9GWGLAQWGGTRR441 pKa = 11.84KK442 pKa = 9.83QGEE445 pKa = 3.6IDD447 pKa = 3.55YY448 pKa = 10.74ANEE451 pKa = 4.11HH452 pKa = 6.21GLDD455 pKa = 3.93PGSLQANIGFMNQEE469 pKa = 3.53LDD471 pKa = 3.31GAYY474 pKa = 10.46SKK476 pKa = 10.02TITDD480 pKa = 3.08IKK482 pKa = 9.51KK483 pKa = 8.43TNNVSDD489 pKa = 3.89AAKK492 pKa = 10.46VWDD495 pKa = 4.14EE496 pKa = 4.6DD497 pKa = 4.09YY498 pKa = 11.77EE499 pKa = 4.34EE500 pKa = 4.48ATDD503 pKa = 3.73PQMGNRR509 pKa = 11.84VQYY512 pKa = 10.23AQNFLDD518 pKa = 3.92EE519 pKa = 4.48GLL521 pKa = 3.94

Molecular weight: 52.62 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6J5DIH3|A0A6J5DIH3_9BURK STAS domain-containing protein OS=Paraburkholderia humisilvae OX=627669 GN=LMG29542_01941 PE=4 SV=1
MM1 pKa = 7.11HH2 pKa = 7.28TLLSTRR8 pKa = 11.84LTRR11 pKa = 11.84AALTAARR18 pKa = 11.84PARR21 pKa = 11.84RR22 pKa = 11.84RR23 pKa = 11.84VVRR26 pKa = 11.84ALRR29 pKa = 11.84HH30 pKa = 4.85HH31 pKa = 5.94SARR34 pKa = 11.84TRR36 pKa = 11.84ALTEE40 pKa = 3.48QWRR43 pKa = 11.84DD44 pKa = 3.16ARR46 pKa = 11.84PVDD49 pKa = 4.84SVRR52 pKa = 11.84TWFRR56 pKa = 11.84TFFSTLRR63 pKa = 11.84MQGASRR69 pKa = 11.84RR70 pKa = 11.84FSLRR74 pKa = 11.84TLLLRR79 pKa = 11.84RR80 pKa = 11.84PTPLRR85 pKa = 11.84VAGMAGGLNLVRR97 pKa = 11.84GLNVARR103 pKa = 11.84GMSRR107 pKa = 11.84KK108 pKa = 9.01PRR110 pKa = 11.84RR111 pKa = 11.84TSTTSTGWFAFARR124 pKa = 4.07

Molecular weight: 14.23 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

8698

0

8698

2609239

29

5463

300.0

32.63

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.536 ± 0.044

0.988 ± 0.009

5.526 ± 0.018

4.95 ± 0.024

3.632 ± 0.016

7.952 ± 0.03

2.427 ± 0.015

4.707 ± 0.019

3.002 ± 0.023

10.006 ± 0.037

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.324 ± 0.014

2.909 ± 0.021

5.165 ± 0.023

3.74 ± 0.017

7.256 ± 0.031

5.882 ± 0.027

5.578 ± 0.025

7.647 ± 0.02

1.372 ± 0.011

2.401 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski