Bacillus phage phiAGATE

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Herelleviridae; Bastillevirinae; Agatevirus; Bacillus virus Agate

Average proteome isoelectric point is 6.0

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 204 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|L0L8E7|L0L8E7_9CAUD Uncharacterized protein OS=Bacillus phage phiAGATE OX=1204533 PE=4 SV=1
MM1 pKa = 7.73FYY3 pKa = 10.08MLEE6 pKa = 4.1TTGYY10 pKa = 8.85RR11 pKa = 11.84LIGILEE17 pKa = 4.48DD18 pKa = 4.09EE19 pKa = 4.38VDD21 pKa = 3.67ALQSLEE27 pKa = 4.08NEE29 pKa = 4.33YY30 pKa = 11.25DD31 pKa = 3.38GNTYY35 pKa = 10.25IGDD38 pKa = 5.35AITEE42 pKa = 4.27IADD45 pKa = 4.03SFSPTYY51 pKa = 10.31DD52 pKa = 3.03SKK54 pKa = 10.86LWKK57 pKa = 9.81NAEE60 pKa = 4.26HH61 pKa = 6.8IEE63 pKa = 4.21EE64 pKa = 4.56YY65 pKa = 10.31IEE67 pKa = 4.05EE68 pKa = 4.34AVSSGLVDD76 pKa = 3.32TSNFNLTKK84 pKa = 9.75MFQAGYY90 pKa = 8.23YY91 pKa = 9.62VYY93 pKa = 9.58YY94 pKa = 10.45TEE96 pKa = 5.32SLYY99 pKa = 11.5KK100 pKa = 10.78NLDD103 pKa = 3.33AVLYY107 pKa = 8.85NYY109 pKa = 10.46SVNLVNEE116 pKa = 4.09EE117 pKa = 4.15LKK119 pKa = 11.02GKK121 pKa = 9.54EE122 pKa = 4.07VSQGVYY128 pKa = 10.58EE129 pKa = 4.99NIAADD134 pKa = 3.98LDD136 pKa = 4.23EE137 pKa = 5.55LCTHH141 pKa = 6.35VNNNDD146 pKa = 3.12TFDD149 pKa = 3.64QFDD152 pKa = 4.15SKK154 pKa = 11.45VMAILEE160 pKa = 4.22EE161 pKa = 4.38HH162 pKa = 5.4TTAA165 pKa = 5.41

Molecular weight: 18.84 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|L0LC53|L0LC53_9CAUD Uncharacterized protein OS=Bacillus phage phiAGATE OX=1204533 PE=4 SV=1
MM1 pKa = 7.61KK2 pKa = 9.99KK3 pKa = 10.14PRR5 pKa = 11.84MRR7 pKa = 11.84AILKK11 pKa = 9.5AKK13 pKa = 9.64GYY15 pKa = 10.04KK16 pKa = 9.8RR17 pKa = 11.84STDD20 pKa = 2.64ICGFRR25 pKa = 11.84VYY27 pKa = 9.37TKK29 pKa = 10.09KK30 pKa = 10.61GHH32 pKa = 6.36PAFLPTTYY40 pKa = 10.29SLKK43 pKa = 10.64VMRR46 pKa = 11.84LLNGLNPNPNEE57 pKa = 3.7VDD59 pKa = 3.05KK60 pKa = 11.59KK61 pKa = 10.36HH62 pKa = 5.51FEE64 pKa = 4.16KK65 pKa = 10.44WFSNYY70 pKa = 10.44DD71 pKa = 3.52FVAKK75 pKa = 10.53RR76 pKa = 11.84SRR78 pKa = 11.84TEE80 pKa = 3.75PAVVAYY86 pKa = 10.28RR87 pKa = 11.84RR88 pKa = 11.84GLRR91 pKa = 11.84KK92 pKa = 9.77RR93 pKa = 11.84NQQ95 pKa = 2.9

Molecular weight: 11.22 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

204

0

204

42709

40

1251

209.4

23.75

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.401 ± 0.19

0.691 ± 0.057

6.399 ± 0.145

8.153 ± 0.317

3.964 ± 0.111

5.996 ± 0.263

1.904 ± 0.087

6.235 ± 0.159

7.483 ± 0.224

8.291 ± 0.167

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.583 ± 0.096

5.413 ± 0.146

3.123 ± 0.168

3.728 ± 0.141

4.662 ± 0.13

6.638 ± 0.168

6.095 ± 0.254

7.01 ± 0.164

0.979 ± 0.066

4.252 ± 0.117
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski