Clostridium sp. CAG:217

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae; Clostridium; environmental samples

Average proteome isoelectric point is 6.36

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1746 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R6IYX9|R6IYX9_9CLOT Pyruvate kinase OS=Clostridium sp. CAG:217 OX=1262779 GN=BN539_01545 PE=3 SV=1
MM1 pKa = 7.38KK2 pKa = 10.25KK3 pKa = 9.67IAIVYY8 pKa = 8.91WSGTGNTAQMAEE20 pKa = 4.17AVAEE24 pKa = 4.33GAGAAGAQANLFTPSDD40 pKa = 4.09FSPAQVKK47 pKa = 10.51DD48 pKa = 3.35YY49 pKa = 11.36DD50 pKa = 4.86AIAFGCPAMGAEE62 pKa = 3.95QLEE65 pKa = 4.49DD66 pKa = 4.38FEE68 pKa = 5.28FEE70 pKa = 4.58PLFLQCAPEE79 pKa = 4.38LNGKK83 pKa = 9.75DD84 pKa = 2.78IALFGSYY91 pKa = 9.01GWGDD95 pKa = 4.25GEE97 pKa = 5.46WMQTWEE103 pKa = 4.65DD104 pKa = 3.42QCKK107 pKa = 10.59DD108 pKa = 3.35LGAHH112 pKa = 6.2LVCNSVIANEE122 pKa = 4.57APDD125 pKa = 4.0DD126 pKa = 3.88TALAACRR133 pKa = 11.84NLGAALAA140 pKa = 4.43

Molecular weight: 14.76 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R6J2B6|R6J2B6_9CLOT PS_pyruv_trans domain-containing protein OS=Clostridium sp. CAG:217 OX=1262779 GN=BN539_00136 PE=4 SV=1
MM1 pKa = 7.46AVTHH5 pKa = 6.43GSLRR9 pKa = 11.84HH10 pKa = 5.36TGFNNIHH17 pKa = 5.7QLEE20 pKa = 4.4CSLNGLFLPGFYY32 pKa = 10.42NISGNAAAEE41 pKa = 4.12FFLPIAVEE49 pKa = 4.29NIRR52 pKa = 11.84QRR54 pKa = 11.84SLVISIDD61 pKa = 4.12HH62 pKa = 6.48ICGAAAGLTHH72 pKa = 6.05THH74 pKa = 4.92IQRR77 pKa = 11.84RR78 pKa = 11.84VLMEE82 pKa = 3.96GEE84 pKa = 4.22TPLRR88 pKa = 11.84RR89 pKa = 11.84IQLKK93 pKa = 10.22RR94 pKa = 11.84ADD96 pKa = 3.91SQVQQHH102 pKa = 6.45TIYY105 pKa = 10.8AAQTPVDD112 pKa = 3.43QYY114 pKa = 11.3VFNIRR119 pKa = 11.84KK120 pKa = 8.01IVVYY124 pKa = 10.11NGHH127 pKa = 6.54IRR129 pKa = 11.84QIVQPSVQHH138 pKa = 5.95RR139 pKa = 11.84NGVRR143 pKa = 11.84VLIHH147 pKa = 6.01CQQVAAAFCNSARR160 pKa = 11.84VTAAPCRR167 pKa = 11.84TVYY170 pKa = 10.63QPFARR175 pKa = 11.84GRR177 pKa = 11.84LHH179 pKa = 6.76GCHH182 pKa = 6.56RR183 pKa = 11.84LCKK186 pKa = 9.97QHH188 pKa = 5.9TLVHH192 pKa = 6.34KK193 pKa = 9.37FHH195 pKa = 7.28LIHH198 pKa = 6.87LPSALPXXXLLFRR211 pKa = 11.84SRR213 pKa = 11.84LRR215 pKa = 11.84FFTGIHH221 pKa = 5.84QIQEE225 pKa = 3.75VGIRR229 pKa = 11.84PQAVEE234 pKa = 4.32HH235 pKa = 6.1GVEE238 pKa = 4.23ALIGPNLNGALVALHH253 pKa = 5.03NHH255 pKa = 5.03GRR257 pKa = 11.84AVFHH261 pKa = 6.35TGIVFQPGIQRR272 pKa = 11.84KK273 pKa = 8.24AALCVQRR280 pKa = 11.84TLLCVGEE287 pKa = 4.25EE288 pKa = 3.89NFVFLIHH295 pKa = 6.26FVSSTVVQRR304 pKa = 11.84LHH306 pKa = 5.92TVHH309 pKa = 7.28KK310 pKa = 10.0LILGIQFDD318 pKa = 4.11TGAAPQVHH326 pKa = 6.13TNHH329 pKa = 6.47KK330 pKa = 9.61KK331 pKa = 10.1FRR333 pKa = 11.84SQAFPPLGRR342 pKa = 11.84NRR344 pKa = 11.84KK345 pKa = 9.42APFAVQLLVHH355 pKa = 6.69ICTHH359 pKa = 7.1KK360 pKa = 10.35DD361 pKa = 3.23TQIHH365 pKa = 5.89KK366 pKa = 10.45RR367 pKa = 11.84RR368 pKa = 11.84LLSAKK373 pKa = 9.53INRR376 pKa = 11.84MHH378 pKa = 6.97LWEE381 pKa = 5.45IPPKK385 pKa = 8.97SQVWLGTVMKK395 pKa = 10.72NDD397 pKa = 4.94GIQWNSHH404 pKa = 4.86HH405 pKa = 7.29SYY407 pKa = 11.36LLL409 pKa = 3.78

Molecular weight: 45.86 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1746

0

1746

554469

29

3359

317.6

35.11

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.52 ± 0.071

1.778 ± 0.027

6.02 ± 0.05

5.711 ± 0.063

3.973 ± 0.043

7.523 ± 0.054

1.923 ± 0.031

5.776 ± 0.065

6.003 ± 0.052

9.195 ± 0.074

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.67 ± 0.027

3.909 ± 0.043

3.844 ± 0.041

3.645 ± 0.041

4.872 ± 0.054

5.193 ± 0.051

6.119 ± 0.065

7.473 ± 0.051

0.872 ± 0.023

3.966 ± 0.047
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski