Arthrobacter phage Grekaycon

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Marthavirus; unclassified Marthavirus

Average proteome isoelectric point is 6.16

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 78 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A514A5H5|A0A514A5H5_9CAUD Uncharacterized protein OS=Arthrobacter phage Grekaycon OX=2591068 GN=29 PE=4 SV=1
MM1 pKa = 7.89ADD3 pKa = 3.48GVLSFPFRR11 pKa = 11.84LTTTGAIATVAYY23 pKa = 10.2GSDD26 pKa = 3.21AFVDD30 pKa = 3.65EE31 pKa = 5.71AIAKK35 pKa = 9.86LVLTNIGEE43 pKa = 4.31RR44 pKa = 11.84PMAPDD49 pKa = 3.53YY50 pKa = 10.83GVPDD54 pKa = 3.65PTFAALHH61 pKa = 6.3IGDD64 pKa = 3.95VQAGLTAFGPTGVRR78 pKa = 11.84VTSVEE83 pKa = 4.04MQPFSDD89 pKa = 3.91TQSVASIAWAYY100 pKa = 10.33EE101 pKa = 3.82DD102 pKa = 5.64ALGDD106 pKa = 3.94TNNGG110 pKa = 3.28

Molecular weight: 11.41 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A514A5K0|A0A514A5K0_9CAUD Uncharacterized protein OS=Arthrobacter phage Grekaycon OX=2591068 GN=55 PE=4 SV=1
MM1 pKa = 7.74TDD3 pKa = 3.1AQPVRR8 pKa = 11.84KK9 pKa = 9.84VKK11 pKa = 9.17ITISFEE17 pKa = 4.09QQDD20 pKa = 3.47IAAIPPLLTTDD31 pKa = 4.06AVPSVLAQRR40 pKa = 11.84VAAHH44 pKa = 6.7ANTYY48 pKa = 8.92MRR50 pKa = 11.84LTKK53 pKa = 9.91HH54 pKa = 5.54ARR56 pKa = 11.84WWRR59 pKa = 11.84IDD61 pKa = 3.08WPAGSRR67 pKa = 11.84KK68 pKa = 10.08GSITSTRR75 pKa = 11.84TGPNRR80 pKa = 11.84DD81 pKa = 3.11KK82 pKa = 11.17PLIGFAAKK90 pKa = 10.28KK91 pKa = 10.06GWW93 pKa = 3.18

Molecular weight: 10.37 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

78

0

78

16038

42

876

205.6

22.44

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.94 ± 0.486

0.667 ± 0.1

6.466 ± 0.312

6.316 ± 0.339

2.974 ± 0.167

7.414 ± 0.403

1.858 ± 0.137

5.057 ± 0.259

4.714 ± 0.182

7.925 ± 0.308

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.369 ± 0.134

3.492 ± 0.179

4.951 ± 0.265

3.81 ± 0.197

6.304 ± 0.345

5.593 ± 0.264

6.703 ± 0.312

7.644 ± 0.297

1.509 ± 0.148

2.295 ± 0.118
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski