Thalassolituus sp. C2-1

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Oceanospirillales; Oceanospirillaceae; Thalassolituus; unclassified Thalassolituus

Average proteome isoelectric point is 6.02

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3803 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A558NQP9|A0A558NQP9_9GAMM Universal stress protein OS=Thalassolituus sp. C2-1 OX=2597518 GN=FOT50_07310 PE=4 SV=1
MM1 pKa = 7.39NSYY4 pKa = 10.58IAMFEE9 pKa = 3.97IPATDD14 pKa = 3.99LARR17 pKa = 11.84AVAFYY22 pKa = 10.55QAVLGVDD29 pKa = 3.97IEE31 pKa = 4.43CLEE34 pKa = 4.22MPDD37 pKa = 3.71MEE39 pKa = 4.86MGILPYY45 pKa = 10.34EE46 pKa = 4.55GQLVPAVIVKK56 pKa = 10.55GEE58 pKa = 4.18GYY60 pKa = 9.89IPSSDD65 pKa = 3.07GVTVYY70 pKa = 10.96LNAGDD75 pKa = 5.21DD76 pKa = 4.22LQPMLDD82 pKa = 3.3RR83 pKa = 11.84VAGSGGQVILEE94 pKa = 4.33KK95 pKa = 9.85TAHH98 pKa = 6.77ADD100 pKa = 3.14NSGFFALFLDD110 pKa = 4.39SEE112 pKa = 4.78GNKK115 pKa = 9.84LALNSANN122 pKa = 3.53

Molecular weight: 12.99 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A558NPW3|A0A558NPW3_9GAMM Diacylglycerol kinase OS=Thalassolituus sp. C2-1 OX=2597518 GN=FOT50_05660 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVLKK11 pKa = 10.49RR12 pKa = 11.84KK13 pKa = 7.97RR14 pKa = 11.84THH16 pKa = 5.89GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.3NGRR28 pKa = 11.84AVLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.15GRR39 pKa = 11.84HH40 pKa = 4.81VLTAA44 pKa = 4.1

Molecular weight: 5.12 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3803

0

3803

1278525

26

5404

336.2

37.15

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.227 ± 0.05

0.991 ± 0.014

5.704 ± 0.037

5.918 ± 0.037

3.755 ± 0.023

7.197 ± 0.038

2.251 ± 0.023

5.383 ± 0.035

3.827 ± 0.037

11.203 ± 0.064

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.486 ± 0.021

3.809 ± 0.036

4.272 ± 0.029

5.159 ± 0.043

5.643 ± 0.036

6.351 ± 0.039

4.969 ± 0.035

6.583 ± 0.037

1.427 ± 0.02

2.844 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski