Paraburkholderia ultramafica

Taxonomy: cellular organisms;

Average proteome isoelectric point is 6.82

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7265 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6S7BLQ1|A0A6S7BLQ1_9BURK Uncharacterized protein OS=Paraburkholderia ultramafica OX=1544867 GN=LMG28614_06085 PE=4 SV=1
MM1 pKa = 8.07DD2 pKa = 4.92NLYY5 pKa = 10.18IAATTTSPEE14 pKa = 3.31VDD16 pKa = 3.92FQFDD20 pKa = 3.73QNVLTFRR27 pKa = 11.84GEE29 pKa = 4.36SYY31 pKa = 10.62PEE33 pKa = 3.55NAAAFYY39 pKa = 10.9APVIEE44 pKa = 4.47RR45 pKa = 11.84LRR47 pKa = 11.84AYY49 pKa = 10.11LASCNDD55 pKa = 2.85AVMTVEE61 pKa = 4.8VSLTYY66 pKa = 10.57FNSSSTKK73 pKa = 9.07MLFSVFDD80 pKa = 4.51ALDD83 pKa = 3.42QAASSGNRR91 pKa = 11.84VLVRR95 pKa = 11.84WYY97 pKa = 10.61RR98 pKa = 11.84DD99 pKa = 3.51AEE101 pKa = 4.27DD102 pKa = 3.5EE103 pKa = 4.52TILEE107 pKa = 4.4FGEE110 pKa = 4.02EE111 pKa = 3.93LQADD115 pKa = 4.73FTAIQFTDD123 pKa = 4.02CPVATT128 pKa = 4.69

Molecular weight: 14.34 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6S7BA28|A0A6S7BA28_9BURK Voltage-gated ClC-type chloride channel ClcB OS=Paraburkholderia ultramafica OX=1544867 GN=clcB_2 PE=4 SV=1
MM1 pKa = 7.22SVHH4 pKa = 6.4RR5 pKa = 11.84PSVARR10 pKa = 11.84GLALNPNAAPRR21 pKa = 11.84RR22 pKa = 11.84RR23 pKa = 11.84LARR26 pKa = 11.84ALLLSGRR33 pKa = 11.84LNMGARR39 pKa = 11.84LRR41 pKa = 11.84AATPVPPGFAMGSARR56 pKa = 11.84RR57 pKa = 11.84LIGARR62 pKa = 11.84SPARR66 pKa = 11.84ISLFLLHH73 pKa = 6.85GG74 pKa = 4.19

Molecular weight: 7.86 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7265

0

7265

2174488

29

2559

299.3

32.56

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.6 ± 0.04

0.984 ± 0.011

5.383 ± 0.025

5.139 ± 0.029

3.714 ± 0.019

7.96 ± 0.028

2.38 ± 0.016

4.737 ± 0.022

3.143 ± 0.023

10.246 ± 0.033

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.38 ± 0.013

2.856 ± 0.018

5.092 ± 0.023

3.718 ± 0.021

7.015 ± 0.031

5.73 ± 0.026

5.399 ± 0.02

7.733 ± 0.03

1.382 ± 0.012

2.409 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski