Amino acid dipepetide frequency for Kineosphaera limosa NBRC 100340

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	21.731AlaAla: 21.731 ± 0.214	1.125AlaCys: 1.125 ± 0.027	8.793AlaAsp: 8.793 ± 0.099	6.702AlaGlu: 6.702 ± 0.087	3.513AlaPhe: 3.513 ± 0.055	13.265AlaGly: 13.265 ± 0.112	2.907AlaHis: 2.907 ± 0.049	5.084AlaIle: 5.084 ± 0.064	2.579AlaLys: 2.579 ± 0.054	14.153AlaLeu: 14.153 ± 0.129	2.985AlaMet: 2.985 ± 0.043	2.288AlaAsn: 2.288 ± 0.037	7.067AlaPro: 7.067 ± 0.092	5.552AlaGln: 5.552 ± 0.074	10.352AlaArg: 10.352 ± 0.098	6.438AlaSer: 6.438 ± 0.087	8.287AlaThr: 8.287 ± 0.098	11.134AlaVal: 11.134 ± 0.105	2.018AlaTrp: 2.018 ± 0.041	2.489AlaTyr: 2.489 ± 0.042	0.0AlaXaa: 0.0 ± 0.0
Cys	1.006CysAla: 1.006 ± 0.029	0.098CysCys: 0.098 ± 0.01	0.46CysAsp: 0.46 ± 0.017	0.386CysGlu: 0.386 ± 0.015	0.199CysPhe: 0.199 ± 0.012	0.898CysGly: 0.898 ± 0.028	0.167CysHis: 0.167 ± 0.011	0.237CysIle: 0.237 ± 0.015	0.087CysLys: 0.087 ± 0.008	0.739CysLeu: 0.739 ± 0.024	0.125CysMet: 0.125 ± 0.009	0.133CysAsn: 0.133 ± 0.012	0.485CysPro: 0.485 ± 0.02	0.166CysGln: 0.166 ± 0.011	0.579CysArg: 0.579 ± 0.019	0.406CysSer: 0.406 ± 0.018	0.435CysThr: 0.435 ± 0.019	0.656CysVal: 0.656 ± 0.02	0.112CysTrp: 0.112 ± 0.01	0.154CysTyr: 0.154 ± 0.01	0.0CysXaa: 0.0 ± 0.0
Asp	8.017AspAla: 8.017 ± 0.08	0.378AspCys: 0.378 ± 0.015	4.127AspAsp: 4.127 ± 0.059	4.107AspGlu: 4.107 ± 0.065	1.532AspPhe: 1.532 ± 0.037	5.719AspGly: 5.719 ± 0.072	1.313AspHis: 1.313 ± 0.03	2.173AspIle: 2.173 ± 0.044	1.005AspLys: 1.005 ± 0.031	6.904AspLeu: 6.904 ± 0.072	0.826AspMet: 0.826 ± 0.026	0.962AspAsn: 0.962 ± 0.029	4.722AspPro: 4.722 ± 0.074	1.702AspGln: 1.702 ± 0.033	4.542AspArg: 4.542 ± 0.066	2.85AspSer: 2.85 ± 0.05	2.76AspThr: 2.76 ± 0.045	5.221AspVal: 5.221 ± 0.066	0.868AspTrp: 0.868 ± 0.024	1.176AspTyr: 1.176 ± 0.027	0.0AspXaa: 0.0 ± 0.0
Glu	7.258GluAla: 7.258 ± 0.081	0.342GluCys: 0.342 ± 0.016	2.257GluAsp: 2.257 ± 0.046	2.638GluGlu: 2.638 ± 0.047	1.427GluPhe: 1.427 ± 0.03	3.909GluGly: 3.909 ± 0.051	1.663GluHis: 1.663 ± 0.039	1.96GluIle: 1.96 ± 0.038	1.039GluLys: 1.039 ± 0.035	6.066GluLeu: 6.066 ± 0.075	0.88GluMet: 0.88 ± 0.023	0.928GluAsn: 0.928 ± 0.027	3.395GluPro: 3.395 ± 0.055	2.586GluGln: 2.586 ± 0.047	5.006GluArg: 5.006 ± 0.068	2.728GluSer: 2.728 ± 0.046	2.427GluThr: 2.427 ± 0.044	4.561GluVal: 4.561 ± 0.066	0.715GluTrp: 0.715 ± 0.022	0.964GluTyr: 0.964 ± 0.026	0.0GluXaa: 0.0 ± 0.0
Phe	3.885PheAla: 3.885 ± 0.057	0.238PheCys: 0.238 ± 0.011	2.062PheAsp: 2.062 ± 0.04	1.531PheGlu: 1.531 ± 0.036	0.979PhePhe: 0.979 ± 0.036	3.14PheGly: 3.14 ± 0.055	0.511PheHis: 0.511 ± 0.019	0.924PheIle: 0.924 ± 0.029	0.42PheLys: 0.42 ± 0.021	2.393PheLeu: 2.393 ± 0.055	0.439PheMet: 0.439 ± 0.02	0.55PheAsn: 0.55 ± 0.022	1.182PhePro: 1.182 ± 0.031	0.576PheGln: 0.576 ± 0.022	1.512PheArg: 1.512 ± 0.033	1.413PheSer: 1.413 ± 0.035	1.808PheThr: 1.808 ± 0.037	2.719PheVal: 2.719 ± 0.049	0.419PheTrp: 0.419 ± 0.019	0.584PheTyr: 0.584 ± 0.02	0.0PheXaa: 0.0 ± 0.0
Gly	11.428GlyAla: 11.428 ± 0.113	0.775GlyCys: 0.775 ± 0.026	5.086GlyAsp: 5.086 ± 0.07	4.64GlyGlu: 4.64 ± 0.062	2.748GlyPhe: 2.748 ± 0.053	8.245GlyGly: 8.245 ± 0.098	2.209GlyHis: 2.209 ± 0.042	3.804GlyIle: 3.804 ± 0.054	1.922GlyLys: 1.922 ± 0.041	9.669GlyLeu: 9.669 ± 0.108	2.077GlyMet: 2.077 ± 0.042	1.711GlyAsn: 1.711 ± 0.035	4.857GlyPro: 4.857 ± 0.068	3.284GlyGln: 3.284 ± 0.05	7.256GlyArg: 7.256 ± 0.081	5.511GlySer: 5.511 ± 0.073	5.361GlyThr: 5.361 ± 0.073	7.839GlyVal: 7.839 ± 0.078	1.655GlyTrp: 1.655 ± 0.036	2.182GlyTyr: 2.182 ± 0.04	0.0GlyXaa: 0.0 ± 0.0
His	2.581HisAla: 2.581 ± 0.049	0.196HisCys: 0.196 ± 0.011	1.529HisAsp: 1.529 ± 0.038	1.291HisGlu: 1.291 ± 0.031	0.545HisPhe: 0.545 ± 0.017	2.224HisGly: 2.224 ± 0.045	0.636HisHis: 0.636 ± 0.023	0.724HisIle: 0.724 ± 0.026	0.331HisLys: 0.331 ± 0.015	2.421HisLeu: 2.421 ± 0.048	0.322HisMet: 0.322 ± 0.015	0.351HisAsn: 0.351 ± 0.016	1.682HisPro: 1.682 ± 0.036	0.577HisGln: 0.577 ± 0.02	1.886HisArg: 1.886 ± 0.04	0.99HisSer: 0.99 ± 0.028	1.17HisThr: 1.17 ± 0.03	1.892HisVal: 1.892 ± 0.034	0.32HisTrp: 0.32 ± 0.013	0.437HisTyr: 0.437 ± 0.015	0.0HisXaa: 0.0 ± 0.0
Ile	5.703IleAla: 5.703 ± 0.063	0.322IleCys: 0.322 ± 0.016	2.866IleAsp: 2.866 ± 0.048	2.565IleGlu: 2.565 ± 0.039	1.013IlePhe: 1.013 ± 0.031	4.036IleGly: 4.036 ± 0.053	0.645IleHis: 0.645 ± 0.022	1.384IleIle: 1.384 ± 0.034	0.659IleLys: 0.659 ± 0.024	2.949IleLeu: 2.949 ± 0.048	0.564IleMet: 0.564 ± 0.021	0.783IleAsn: 0.783 ± 0.025	1.963IlePro: 1.963 ± 0.036	0.772IleGln: 0.772 ± 0.025	2.228IleArg: 2.228 ± 0.037	1.861IleSer: 1.861 ± 0.042	2.409IleThr: 2.409 ± 0.039	3.995IleVal: 3.995 ± 0.056	0.44IleTrp: 0.44 ± 0.017	0.681IleTyr: 0.681 ± 0.022	0.0IleXaa: 0.0 ± 0.0
Lys	2.428LysAla: 2.428 ± 0.054	0.097LysCys: 0.097 ± 0.009	0.986LysAsp: 0.986 ± 0.028	0.994LysGlu: 0.994 ± 0.03	0.428LysPhe: 0.428 ± 0.017	1.48LysGly: 1.48 ± 0.038	0.419LysHis: 0.419 ± 0.017	0.783LysIle: 0.783 ± 0.025	0.605LysLys: 0.605 ± 0.025	1.507LysLeu: 1.507 ± 0.032	0.342LysMet: 0.342 ± 0.016	0.406LysAsn: 0.406 ± 0.018	1.131LysPro: 1.131 ± 0.034	0.595LysGln: 0.595 ± 0.021	1.299LysArg: 1.299 ± 0.037	0.955LysSer: 0.955 ± 0.03	1.095LysThr: 1.095 ± 0.033	1.571LysVal: 1.571 ± 0.043	0.205LysTrp: 0.205 ± 0.013	0.387LysTyr: 0.387 ± 0.017	0.0LysXaa: 0.0 ± 0.0
Leu	15.635LeuAla: 15.635 ± 0.137	0.727LeuCys: 0.727 ± 0.022	6.649LeuAsp: 6.649 ± 0.072	4.787LeuGlu: 4.787 ± 0.066	2.603LeuPhe: 2.603 ± 0.053	9.471LeuGly: 9.471 ± 0.087	2.078LeuHis: 2.078 ± 0.043	3.672LeuIle: 3.672 ± 0.064	1.531LeuLys: 1.531 ± 0.043	11.028LeuLeu: 11.028 ± 0.132	1.718LeuMet: 1.718 ± 0.043	1.606LeuAsn: 1.606 ± 0.031	6.079LeuPro: 6.079 ± 0.061	2.58LeuGln: 2.58 ± 0.04	8.298LeuArg: 8.298 ± 0.071	5.3LeuSer: 5.3 ± 0.065	6.599LeuThr: 6.599 ± 0.075	9.412LeuVal: 9.412 ± 0.112	1.266LeuTrp: 1.266 ± 0.028	1.539LeuTyr: 1.539 ± 0.033	0.0LeuXaa: 0.0 ± 0.0
Met	2.461MetAla: 2.461 ± 0.043	0.154MetCys: 0.154 ± 0.011	0.815MetAsp: 0.815 ± 0.024	0.767MetGlu: 0.767 ± 0.023	0.557MetPhe: 0.557 ± 0.019	1.439MetGly: 1.439 ± 0.034	0.385MetHis: 0.385 ± 0.016	0.792MetIle: 0.792 ± 0.022	0.377MetLys: 0.377 ± 0.015	2.018MetLeu: 2.018 ± 0.043	0.347MetMet: 0.347 ± 0.019	0.47MetAsn: 0.47 ± 0.02	1.186MetPro: 1.186 ± 0.031	0.496MetGln: 0.496 ± 0.019	1.55MetArg: 1.55 ± 0.034	1.661MetSer: 1.661 ± 0.032	1.696MetThr: 1.696 ± 0.036	1.377MetVal: 1.377 ± 0.032	0.256MetTrp: 0.256 ± 0.013	0.306MetTyr: 0.306 ± 0.016	0.0MetXaa: 0.0 ± 0.0
Asn	2.301AsnAla: 2.301 ± 0.041	0.142AsnCys: 0.142 ± 0.009	1.043AsnAsp: 1.043 ± 0.024	0.89AsnGlu: 0.89 ± 0.026	0.476AsnPhe: 0.476 ± 0.018	1.605AsnGly: 1.605 ± 0.039	0.394AsnHis: 0.394 ± 0.016	0.722AsnIle: 0.722 ± 0.022	0.352AsnLys: 0.352 ± 0.017	1.76AsnLeu: 1.76 ± 0.042	0.333AsnMet: 0.333 ± 0.017	0.382AsnAsn: 0.382 ± 0.019	1.531AsnPro: 1.531 ± 0.029	0.551AsnGln: 0.551 ± 0.018	1.251AsnArg: 1.251 ± 0.037	0.937AsnSer: 0.937 ± 0.027	1.027AsnThr: 1.027 ± 0.026	1.49AsnVal: 1.49 ± 0.034	0.233AsnTrp: 0.233 ± 0.013	0.415AsnTyr: 0.415 ± 0.017	0.0AsnXaa: 0.0 ± 0.0
Pro	8.237ProAla: 8.237 ± 0.089	0.321ProCys: 0.321 ± 0.015	4.198ProAsp: 4.198 ± 0.054	3.779ProGlu: 3.779 ± 0.06	1.482ProPhe: 1.482 ± 0.036	6.209ProGly: 6.209 ± 0.074	1.281ProHis: 1.281 ± 0.03	1.983ProIle: 1.983 ± 0.037	1.084ProLys: 1.084 ± 0.027	4.992ProLeu: 4.992 ± 0.07	1.181ProMet: 1.181 ± 0.025	1.034ProAsn: 1.034 ± 0.028	3.352ProPro: 3.352 ± 0.062	2.226ProGln: 2.226 ± 0.045	3.954ProArg: 3.954 ± 0.054	3.155ProSer: 3.155 ± 0.053	4.078ProThr: 4.078 ± 0.065	5.064ProVal: 5.064 ± 0.064	0.966ProTrp: 0.966 ± 0.027	1.027ProTyr: 1.027 ± 0.026	0.0ProXaa: 0.0 ± 0.0
Gln	4.767GlnAla: 4.767 ± 0.063	0.199GlnCys: 0.199 ± 0.011	1.375GlnAsp: 1.375 ± 0.03	1.733GlnGlu: 1.733 ± 0.037	0.762GlnPhe: 0.762 ± 0.024	2.43GlnGly: 2.43 ± 0.046	0.758GlnHis: 0.758 ± 0.023	1.465GlnIle: 1.465 ± 0.035	0.46GlnLys: 0.46 ± 0.017	3.524GlnLeu: 3.524 ± 0.06	0.622GlnMet: 0.622 ± 0.024	0.536GlnAsn: 0.536 ± 0.019	1.881GlnPro: 1.881 ± 0.042	1.36GlnGln: 1.36 ± 0.036	3.04GlnArg: 3.04 ± 0.052	1.459GlnSer: 1.459 ± 0.032	1.691GlnThr: 1.691 ± 0.036	3.278GlnVal: 3.278 ± 0.041	0.527GlnTrp: 0.527 ± 0.018	0.548GlnTyr: 0.548 ± 0.02	0.0GlnXaa: 0.0 ± 0.0
Arg	9.785ArgAla: 9.785 ± 0.095	0.546ArgCys: 0.546 ± 0.022	4.592ArgAsp: 4.592 ± 0.064	4.494ArgGlu: 4.494 ± 0.052	2.251ArgPhe: 2.251 ± 0.039	5.916ArgGly: 5.916 ± 0.07	1.956ArgHis: 1.956 ± 0.044	3.191ArgIle: 3.191 ± 0.049	1.273ArgLys: 1.273 ± 0.033	8.37ArgLeu: 8.37 ± 0.092	1.813ArgMet: 1.813 ± 0.039	1.287ArgAsn: 1.287 ± 0.03	4.64ArgPro: 4.64 ± 0.056	2.297ArgGln: 2.297 ± 0.041	7.55ArgArg: 7.55 ± 0.084	4.2ArgSer: 4.2 ± 0.059	4.334ArgThr: 4.334 ± 0.052	6.185ArgVal: 6.185 ± 0.06	1.361ArgTrp: 1.361 ± 0.031	1.612ArgTyr: 1.612 ± 0.034	0.0ArgXaa: 0.0 ± 0.0
Ser	7.203SerAla: 7.203 ± 0.085	0.378SerCys: 0.378 ± 0.016	2.934SerAsp: 2.934 ± 0.038	2.329SerGlu: 2.329 ± 0.045	1.483SerPhe: 1.483 ± 0.033	5.84SerGly: 5.84 ± 0.07	0.986SerHis: 0.986 ± 0.027	1.953SerIle: 1.953 ± 0.039	0.898SerLys: 0.898 ± 0.029	4.899SerLeu: 4.899 ± 0.059	1.271SerMet: 1.271 ± 0.028	0.987SerAsn: 0.987 ± 0.03	3.31SerPro: 3.31 ± 0.054	1.571SerGln: 1.571 ± 0.036	3.831SerArg: 3.831 ± 0.057	2.972SerSer: 2.972 ± 0.054	3.503SerThr: 3.503 ± 0.059	4.298SerVal: 4.298 ± 0.065	0.88SerTrp: 0.88 ± 0.023	1.031SerTyr: 1.031 ± 0.029	0.0SerXaa: 0.0 ± 0.0
Thr	7.558ThrAla: 7.558 ± 0.099	0.472ThrCys: 0.472 ± 0.018	3.589ThrAsp: 3.589 ± 0.053	2.53ThrGlu: 2.53 ± 0.043	1.799ThrPhe: 1.799 ± 0.039	5.873ThrGly: 5.873 ± 0.066	1.225ThrHis: 1.225 ± 0.03	2.493ThrIle: 2.493 ± 0.052	1.155ThrLys: 1.155 ± 0.033	5.797ThrLeu: 5.797 ± 0.072	1.199ThrMet: 1.199 ± 0.027	1.148ThrAsn: 1.148 ± 0.03	4.245ThrPro: 4.245 ± 0.066	2.005ThrGln: 2.005 ± 0.035	4.062ThrArg: 4.062 ± 0.062	3.493ThrSer: 3.493 ± 0.056	3.907ThrThr: 3.907 ± 0.069	5.14ThrVal: 5.14 ± 0.069	0.996ThrTrp: 0.996 ± 0.025	1.263ThrTyr: 1.263 ± 0.028	0.0ThrXaa: 0.0 ± 0.0
Val	12.406ValAla: 12.406 ± 0.117	0.722ValCys: 0.722 ± 0.024	5.492ValAsp: 5.492 ± 0.067	4.861ValGlu: 4.861 ± 0.062	2.394ValPhe: 2.394 ± 0.047	7.551ValGly: 7.551 ± 0.089	1.861ValHis: 1.861 ± 0.038	3.397ValIle: 3.397 ± 0.055	1.378ValLys: 1.378 ± 0.039	9.336ValLeu: 9.336 ± 0.109	1.517ValMet: 1.517 ± 0.036	1.572ValAsn: 1.572 ± 0.035	4.995ValPro: 4.995 ± 0.058	2.353ValGln: 2.353 ± 0.045	6.476ValArg: 6.476 ± 0.08	4.367ValSer: 4.367 ± 0.065	5.512ValThr: 5.512 ± 0.06	8.823ValVal: 8.823 ± 0.094	1.126ValTrp: 1.126 ± 0.026	1.407ValTyr: 1.407 ± 0.03	0.0ValXaa: 0.0 ± 0.0
Trp	1.676TrpAla: 1.676 ± 0.035	0.139TrpCys: 0.139 ± 0.011	0.733TrpAsp: 0.733 ± 0.021	0.67TrpGlu: 0.67 ± 0.021	0.498TrpPhe: 0.498 ± 0.018	1.123TrpGly: 1.123 ± 0.027	0.366TrpHis: 0.366 ± 0.016	0.582TrpIle: 0.582 ± 0.02	0.231TrpLys: 0.231 ± 0.013	1.864TrpLeu: 1.864 ± 0.042	0.327TrpMet: 0.327 ± 0.017	0.337TrpAsn: 0.337 ± 0.017	0.846TrpPro: 0.846 ± 0.025	0.643TrpGln: 0.643 ± 0.022	1.401TrpArg: 1.401 ± 0.037	0.94TrpSer: 0.94 ± 0.024	0.838TrpThr: 0.838 ± 0.024	1.196TrpVal: 1.196 ± 0.033	0.381TrpTrp: 0.381 ± 0.019	0.254TrpTyr: 0.254 ± 0.014	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.396TyrAla: 2.396 ± 0.039	0.155TyrCys: 0.155 ± 0.011	1.318TyrAsp: 1.318 ± 0.029	1.058TyrGlu: 1.058 ± 0.028	0.598TyrPhe: 0.598 ± 0.02	1.886TyrGly: 1.886 ± 0.036	0.348TyrHis: 0.348 ± 0.016	0.514TyrIle: 0.514 ± 0.02	0.327TyrLys: 0.327 ± 0.016	2.191TyrLeu: 2.191 ± 0.034	0.22TyrMet: 0.22 ± 0.012	0.365TyrAsn: 0.365 ± 0.016	1.054TyrPro: 1.054 ± 0.028	0.521TyrGln: 0.521 ± 0.021	1.58TyrArg: 1.58 ± 0.033	0.922TyrSer: 0.922 ± 0.026	1.011TyrThr: 1.011 ± 0.025	1.692TyrVal: 1.692 ± 0.035	0.301TyrTrp: 0.301 ± 0.015	0.41TyrTyr: 0.41 ± 0.017	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 4422 proteins (1442572 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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