Tenacibaculum sp. MAR_2009_124

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Flavobacteriia; Flavobacteriales; Flavobacteriaceae; Tenacibaculum; unclassified Tenacibaculum

Average proteome isoelectric point is 6.86

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4480 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1H4JS34|A0A1H4JS34_9FLAO Uncharacterized protein OS=Tenacibaculum sp. MAR_2009_124 OX=1250059 GN=SAMN04489761_0998 PE=4 SV=1
MM1 pKa = 6.13VTFVFVLGFIACEE14 pKa = 4.1DD15 pKa = 3.64NDD17 pKa = 3.74NLEE20 pKa = 4.36FTVQAPQDD28 pKa = 3.73SVAFTNTLLNEE39 pKa = 4.36YY40 pKa = 9.59IITPQTSGNIAEE52 pKa = 4.08RR53 pKa = 11.84FVWNSVVFIGTATEE67 pKa = 3.92VTYY70 pKa = 10.73DD71 pKa = 3.69LQVSITQDD79 pKa = 3.24FASFEE84 pKa = 4.6SLGTTNDD91 pKa = 2.66THH93 pKa = 6.91MSVTVDD99 pKa = 3.26KK100 pKa = 10.98LLALAEE106 pKa = 4.25TAGLDD111 pKa = 3.94NDD113 pKa = 4.17PTTDD117 pKa = 3.87DD118 pKa = 4.36KK119 pKa = 11.91PNMGLVYY126 pKa = 9.79FRR128 pKa = 11.84VRR130 pKa = 11.84AFVGNGGTDD139 pKa = 3.39APEE142 pKa = 4.71SISDD146 pKa = 3.41IMVMNITLPEE156 pKa = 3.86ITQGSGIEE164 pKa = 3.85ISSWGIVGSGYY175 pKa = 10.6NDD177 pKa = 3.48WGNAGPDD184 pKa = 3.23APFYY188 pKa = 7.42TTNTANVLVAYY199 pKa = 7.51VTLLDD204 pKa = 3.99GEE206 pKa = 4.41IKK208 pKa = 10.58LRR210 pKa = 11.84EE211 pKa = 4.04NNEE214 pKa = 3.65WTNNLGDD221 pKa = 4.82DD222 pKa = 4.27GADD225 pKa = 3.3GTLEE229 pKa = 3.89VGGANIVSSAGTYY242 pKa = 10.29KK243 pKa = 9.28ITLDD247 pKa = 4.27LNTNTYY253 pKa = 9.63TIEE256 pKa = 4.16SYY258 pKa = 10.23SWGIIGSGFNDD269 pKa = 3.86WGNDD273 pKa = 3.52GPDD276 pKa = 3.19AEE278 pKa = 4.3FHH280 pKa = 6.48YY281 pKa = 10.6DD282 pKa = 3.36YY283 pKa = 8.57TTDD286 pKa = 3.31TFKK289 pKa = 11.38VGVKK293 pKa = 10.51LLDD296 pKa = 3.65GEE298 pKa = 4.58IKK300 pKa = 10.42FRR302 pKa = 11.84LNNNWTTNYY311 pKa = 10.6GGTDD315 pKa = 3.31GNLAAGGDD323 pKa = 4.3NIVSTAGFYY332 pKa = 10.51QVTIDD337 pKa = 4.31FNNNTYY343 pKa = 9.92TIEE346 pKa = 4.47ANDD349 pKa = 3.11VWGIIGSGYY358 pKa = 10.11NDD360 pKa = 3.75WGNNGPDD367 pKa = 3.61FNFTQVNPGIYY378 pKa = 9.34IANNVTLLDD387 pKa = 3.84GEE389 pKa = 4.39IKK391 pKa = 10.44FRR393 pKa = 11.84LNEE396 pKa = 3.87DD397 pKa = 2.81WTTNFGDD404 pKa = 4.58DD405 pKa = 4.27GNDD408 pKa = 3.38GTLEE412 pKa = 4.12AEE414 pKa = 4.67GANIPSTAGKK424 pKa = 9.95FRR426 pKa = 11.84ITMDD430 pKa = 4.09LSDD433 pKa = 4.59NSNPTYY439 pKa = 10.26TISSLL444 pKa = 3.53

Molecular weight: 48.1 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1H4IZP7|A0A1H4IZP7_9FLAO Phosphoribosylaminoimidazole-succinocarboxamide synthase OS=Tenacibaculum sp. MAR_2009_124 OX=1250059 GN=purC PE=3 SV=1
MM1 pKa = 7.74SKK3 pKa = 8.95RR4 pKa = 11.84TYY6 pKa = 10.24QPSKK10 pKa = 9.01RR11 pKa = 11.84KK12 pKa = 9.47RR13 pKa = 11.84RR14 pKa = 11.84NKK16 pKa = 9.49HH17 pKa = 3.94GFRR20 pKa = 11.84EE21 pKa = 4.27RR22 pKa = 11.84MASANGRR29 pKa = 11.84KK30 pKa = 9.04VLARR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84AKK38 pKa = 9.73GRR40 pKa = 11.84KK41 pKa = 8.36RR42 pKa = 11.84LTVSSQARR50 pKa = 11.84PKK52 pKa = 10.34KK53 pKa = 10.45

Molecular weight: 6.31 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4480

0

4480

1587372

28

17153

354.3

40.04

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.424 ± 0.043

0.8 ± 0.016

5.638 ± 0.101

6.697 ± 0.06

5.147 ± 0.048

6.319 ± 0.057

1.725 ± 0.02

8.055 ± 0.045

8.145 ± 0.102

9.107 ± 0.069

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.889 ± 0.022

6.847 ± 0.044

3.213 ± 0.03

3.199 ± 0.026

3.26 ± 0.037

7.101 ± 0.045

6.061 ± 0.098

6.188 ± 0.04

1.015 ± 0.014

4.17 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski