Amino acid dipepetide frequency for Streptococcus phage IPP9

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
2.888AlaAla: 2.888 ± 0.674
0.39AlaCys: 0.39 ± 0.189
5.542AlaAsp: 5.542 ± 0.584
6.322AlaGlu: 6.322 ± 0.696
2.264AlaPhe: 2.264 ± 0.688
5.308AlaGly: 5.308 ± 1.177
0.702AlaHis: 0.702 ± 0.179
4.449AlaIle: 4.449 ± 0.9
6.634AlaLys: 6.634 ± 0.713
6.869AlaLeu: 6.869 ± 0.823
2.342AlaMet: 2.342 ± 0.459
3.746AlaAsn: 3.746 ± 0.704
1.795AlaPro: 1.795 ± 0.42
2.185AlaGln: 2.185 ± 0.463
2.576AlaArg: 2.576 ± 0.427
2.576AlaSer: 2.576 ± 0.647
4.293AlaThr: 4.293 ± 0.691
5.464AlaVal: 5.464 ± 0.663
1.171AlaTrp: 1.171 ± 0.421
1.795AlaTyr: 1.795 ± 0.341
0.0AlaXaa: 0.0 ± 0.0
Cys
0.156CysAla: 0.156 ± 0.095
0.078CysCys: 0.078 ± 0.075
0.468CysAsp: 0.468 ± 0.174
0.234CysGlu: 0.234 ± 0.114
0.468CysPhe: 0.468 ± 0.222
0.234CysGly: 0.234 ± 0.138
0.078CysHis: 0.078 ± 0.095
0.546CysIle: 0.546 ± 0.267
0.702CysLys: 0.702 ± 0.284
0.39CysLeu: 0.39 ± 0.165
0.0CysMet: 0.0 ± 0.0
0.234CysAsn: 0.234 ± 0.178
0.234CysPro: 0.234 ± 0.152
0.234CysGln: 0.234 ± 0.143
0.39CysArg: 0.39 ± 0.15
0.39CysSer: 0.39 ± 0.145
0.078CysThr: 0.078 ± 0.077
0.156CysVal: 0.156 ± 0.12
0.156CysTrp: 0.156 ± 0.11
0.468CysTyr: 0.468 ± 0.19
0.0CysXaa: 0.0 ± 0.0
Asp
3.668AspAla: 3.668 ± 0.717
0.624AspCys: 0.624 ± 0.246
3.356AspAsp: 3.356 ± 0.687
4.761AspGlu: 4.761 ± 0.915
3.278AspPhe: 3.278 ± 0.519
4.605AspGly: 4.605 ± 0.597
0.468AspHis: 0.468 ± 0.219
5.542AspIle: 5.542 ± 0.737
5.308AspLys: 5.308 ± 0.705
5.229AspLeu: 5.229 ± 0.723
1.795AspMet: 1.795 ± 0.342
2.498AspAsn: 2.498 ± 0.338
1.795AspPro: 1.795 ± 0.413
1.561AspGln: 1.561 ± 0.375
2.888AspArg: 2.888 ± 0.438
3.903AspSer: 3.903 ± 0.515
3.512AspThr: 3.512 ± 0.493
3.59AspVal: 3.59 ± 0.495
1.483AspTrp: 1.483 ± 0.354
2.81AspTyr: 2.81 ± 0.451
0.0AspXaa: 0.0 ± 0.0
Glu
6.4GluAla: 6.4 ± 0.95
0.156GluCys: 0.156 ± 0.095
4.059GluAsp: 4.059 ± 0.586
5.932GluGlu: 5.932 ± 0.903
3.668GluPhe: 3.668 ± 0.618
3.59GluGly: 3.59 ± 0.517
1.327GluHis: 1.327 ± 0.372
5.698GluIle: 5.698 ± 0.597
7.493GluLys: 7.493 ± 1.384
8.742GluLeu: 8.742 ± 0.932
2.264GluMet: 2.264 ± 0.524
4.449GluAsn: 4.449 ± 0.445
1.405GluPro: 1.405 ± 0.411
3.122GluGln: 3.122 ± 0.521
4.137GluArg: 4.137 ± 0.584
4.761GluSer: 4.761 ± 0.672
4.059GluThr: 4.059 ± 0.55
5.308GluVal: 5.308 ± 0.727
1.093GluTrp: 1.093 ± 0.26
3.356GluTyr: 3.356 ± 0.561
0.0GluXaa: 0.0 ± 0.0
Phe
2.81PheAla: 2.81 ± 0.589
0.312PheCys: 0.312 ± 0.153
4.215PheAsp: 4.215 ± 0.593
4.059PheGlu: 4.059 ± 0.669
1.795PhePhe: 1.795 ± 0.39
2.654PheGly: 2.654 ± 0.665
0.234PheHis: 0.234 ± 0.115
1.873PheIle: 1.873 ± 0.401
3.122PheLys: 3.122 ± 0.639
2.342PheLeu: 2.342 ± 0.392
1.093PheMet: 1.093 ± 0.29
2.732PheAsn: 2.732 ± 0.606
0.624PhePro: 0.624 ± 0.25
1.093PheGln: 1.093 ± 0.293
1.561PheArg: 1.561 ± 0.267
3.122PheSer: 3.122 ± 0.627
2.42PheThr: 2.42 ± 0.433
1.717PheVal: 1.717 ± 0.319
0.781PheTrp: 0.781 ± 0.268
1.561PheTyr: 1.561 ± 0.386
0.0PheXaa: 0.0 ± 0.0
Gly
2.966GlyAla: 2.966 ± 0.583
0.156GlyCys: 0.156 ± 0.126
3.981GlyAsp: 3.981 ± 0.625
4.683GlyGlu: 4.683 ± 0.697
2.81GlyPhe: 2.81 ± 0.539
4.605GlyGly: 4.605 ± 1.085
0.624GlyHis: 0.624 ± 0.183
3.356GlyIle: 3.356 ± 0.525
5.073GlyLys: 5.073 ± 0.513
6.01GlyLeu: 6.01 ± 0.997
1.561GlyMet: 1.561 ± 0.287
3.668GlyAsn: 3.668 ± 0.582
0.859GlyPro: 0.859 ± 0.269
3.356GlyGln: 3.356 ± 0.537
3.122GlyArg: 3.122 ± 0.529
3.512GlySer: 3.512 ± 0.87
2.576GlyThr: 2.576 ± 0.497
4.839GlyVal: 4.839 ± 0.76
1.015GlyTrp: 1.015 ± 0.391
3.044GlyTyr: 3.044 ± 0.423
0.0GlyXaa: 0.0 ± 0.0
His
0.624HisAla: 0.624 ± 0.217
0.078HisCys: 0.078 ± 0.08
0.781HisAsp: 0.781 ± 0.318
1.405HisGlu: 1.405 ± 0.354
0.702HisPhe: 0.702 ± 0.254
0.781HisGly: 0.781 ± 0.32
0.234HisHis: 0.234 ± 0.159
0.937HisIle: 0.937 ± 0.314
0.624HisLys: 0.624 ± 0.341
1.249HisLeu: 1.249 ± 0.377
0.156HisMet: 0.156 ± 0.106
1.093HisAsn: 1.093 ± 0.269
0.781HisPro: 0.781 ± 0.251
0.546HisGln: 0.546 ± 0.182
0.702HisArg: 0.702 ± 0.232
1.093HisSer: 1.093 ± 0.35
0.859HisThr: 0.859 ± 0.253
0.702HisVal: 0.702 ± 0.242
0.156HisTrp: 0.156 ± 0.115
0.781HisTyr: 0.781 ± 0.278
0.0HisXaa: 0.0 ± 0.0
Ile
5.464IleAla: 5.464 ± 0.628
0.546IleCys: 0.546 ± 0.186
3.668IleAsp: 3.668 ± 0.549
6.088IleGlu: 6.088 ± 0.701
2.966IlePhe: 2.966 ± 0.526
3.825IleGly: 3.825 ± 0.752
0.312IleHis: 0.312 ± 0.164
2.654IleIle: 2.654 ± 0.452
6.478IleLys: 6.478 ± 0.682
3.825IleLeu: 3.825 ± 0.555
1.483IleMet: 1.483 ± 0.397
3.278IleAsn: 3.278 ± 0.509
1.405IlePro: 1.405 ± 0.388
2.42IleGln: 2.42 ± 0.331
2.654IleArg: 2.654 ± 0.453
4.605IleSer: 4.605 ± 0.6
4.605IleThr: 4.605 ± 0.585
3.434IleVal: 3.434 ± 0.53
0.546IleTrp: 0.546 ± 0.227
2.576IleTyr: 2.576 ± 0.667
0.0IleXaa: 0.0 ± 0.0
Lys
5.542LysAla: 5.542 ± 0.612
0.312LysCys: 0.312 ± 0.164
5.854LysAsp: 5.854 ± 0.663
7.415LysGlu: 7.415 ± 0.966
2.966LysPhe: 2.966 ± 0.629
4.839LysGly: 4.839 ± 0.627
1.795LysHis: 1.795 ± 0.378
6.01LysIle: 6.01 ± 0.801
8.43LysLys: 8.43 ± 1.023
7.337LysLeu: 7.337 ± 0.699
2.654LysMet: 2.654 ± 0.401
4.761LysAsn: 4.761 ± 0.556
2.42LysPro: 2.42 ± 0.528
3.512LysGln: 3.512 ± 0.568
3.746LysArg: 3.746 ± 0.46
4.371LysSer: 4.371 ± 0.548
6.01LysThr: 6.01 ± 0.674
5.776LysVal: 5.776 ± 0.579
1.093LysTrp: 1.093 ± 0.349
3.903LysTyr: 3.903 ± 0.634
0.0LysXaa: 0.0 ± 0.0
Leu
7.103LeuAla: 7.103 ± 0.716
0.468LeuCys: 0.468 ± 0.247
5.62LeuAsp: 5.62 ± 0.64
7.415LeuGlu: 7.415 ± 0.941
2.888LeuPhe: 2.888 ± 0.387
5.464LeuGly: 5.464 ± 1.233
1.171LeuHis: 1.171 ± 0.248
4.059LeuIle: 4.059 ± 0.544
7.649LeuLys: 7.649 ± 0.846
6.166LeuLeu: 6.166 ± 0.794
2.107LeuMet: 2.107 ± 0.337
3.278LeuAsn: 3.278 ± 0.529
2.81LeuPro: 2.81 ± 0.495
3.122LeuGln: 3.122 ± 0.607
3.512LeuArg: 3.512 ± 0.535
5.62LeuSer: 5.62 ± 0.877
5.698LeuThr: 5.698 ± 0.904
4.293LeuVal: 4.293 ± 0.558
0.546LeuTrp: 0.546 ± 0.18
2.654LeuTyr: 2.654 ± 0.391
0.0LeuXaa: 0.0 ± 0.0
Met
1.639MetAla: 1.639 ± 0.357
0.0MetCys: 0.0 ± 0.0
1.405MetAsp: 1.405 ± 0.271
2.107MetGlu: 2.107 ± 0.478
0.781MetPhe: 0.781 ± 0.211
1.093MetGly: 1.093 ± 0.45
0.234MetHis: 0.234 ± 0.15
1.795MetIle: 1.795 ± 0.403
2.498MetLys: 2.498 ± 0.527
1.951MetLeu: 1.951 ± 0.396
0.39MetMet: 0.39 ± 0.217
1.873MetAsn: 1.873 ± 0.482
0.937MetPro: 0.937 ± 0.299
0.937MetGln: 0.937 ± 0.325
1.327MetArg: 1.327 ± 0.315
1.327MetSer: 1.327 ± 0.392
1.951MetThr: 1.951 ± 0.394
1.639MetVal: 1.639 ± 0.32
0.234MetTrp: 0.234 ± 0.155
0.781MetTyr: 0.781 ± 0.228
0.0MetXaa: 0.0 ± 0.0
Asn
4.371AsnAla: 4.371 ± 0.717
0.312AsnCys: 0.312 ± 0.139
2.966AsnAsp: 2.966 ± 0.482
2.966AsnGlu: 2.966 ± 0.61
2.107AsnPhe: 2.107 ± 0.47
3.903AsnGly: 3.903 ± 0.489
1.015AsnHis: 1.015 ± 0.311
2.966AsnIle: 2.966 ± 0.556
4.995AsnLys: 4.995 ± 0.542
4.839AsnLeu: 4.839 ± 0.527
1.171AsnMet: 1.171 ± 0.333
2.888AsnAsn: 2.888 ± 0.59
1.951AsnPro: 1.951 ± 0.418
2.966AsnGln: 2.966 ± 0.56
2.654AsnArg: 2.654 ± 0.478
3.278AsnSer: 3.278 ± 0.585
2.732AsnThr: 2.732 ± 0.528
3.2AsnVal: 3.2 ± 0.392
0.781AsnTrp: 0.781 ± 0.235
2.107AsnTyr: 2.107 ± 0.405
0.0AsnXaa: 0.0 ± 0.0
Pro
2.185ProAla: 2.185 ± 0.531
0.234ProCys: 0.234 ± 0.155
2.107ProAsp: 2.107 ± 0.537
3.044ProGlu: 3.044 ± 0.395
0.546ProPhe: 0.546 ± 0.222
0.937ProGly: 0.937 ± 0.181
0.312ProHis: 0.312 ± 0.123
1.639ProIle: 1.639 ± 0.412
2.888ProLys: 2.888 ± 0.396
1.327ProLeu: 1.327 ± 0.371
0.546ProMet: 0.546 ± 0.207
1.561ProAsn: 1.561 ± 0.524
0.702ProPro: 0.702 ± 0.299
0.859ProGln: 0.859 ± 0.297
1.171ProArg: 1.171 ± 0.294
1.483ProSer: 1.483 ± 0.456
0.937ProThr: 0.937 ± 0.319
1.951ProVal: 1.951 ± 0.387
0.468ProTrp: 0.468 ± 0.189
1.639ProTyr: 1.639 ± 0.442
0.0ProXaa: 0.0 ± 0.0
Gln
3.981GlnAla: 3.981 ± 0.533
0.156GlnCys: 0.156 ± 0.098
1.405GlnAsp: 1.405 ± 0.288
3.278GlnGlu: 3.278 ± 0.615
1.483GlnPhe: 1.483 ± 0.301
1.795GlnGly: 1.795 ± 0.314
0.624GlnHis: 0.624 ± 0.243
3.122GlnIle: 3.122 ± 0.489
3.746GlnLys: 3.746 ± 0.497
3.122GlnLeu: 3.122 ± 0.389
0.859GlnMet: 0.859 ± 0.206
1.561GlnAsn: 1.561 ± 0.378
1.327GlnPro: 1.327 ± 0.297
1.561GlnGln: 1.561 ± 0.364
1.717GlnArg: 1.717 ± 0.397
2.732GlnSer: 2.732 ± 0.377
3.044GlnThr: 3.044 ± 0.559
3.434GlnVal: 3.434 ± 0.485
0.624GlnTrp: 0.624 ± 0.204
1.093GlnTyr: 1.093 ± 0.33
0.0GlnXaa: 0.0 ± 0.0
Arg
2.654ArgAla: 2.654 ± 0.493
0.312ArgCys: 0.312 ± 0.167
2.42ArgAsp: 2.42 ± 0.434
2.888ArgGlu: 2.888 ± 0.527
1.405ArgPhe: 1.405 ± 0.374
1.561ArgGly: 1.561 ± 0.354
0.781ArgHis: 0.781 ± 0.298
2.498ArgIle: 2.498 ± 0.558
3.512ArgLys: 3.512 ± 0.751
4.839ArgLeu: 4.839 ± 0.745
2.264ArgMet: 2.264 ± 0.489
2.888ArgAsn: 2.888 ± 0.532
0.781ArgPro: 0.781 ± 0.241
2.264ArgGln: 2.264 ± 0.486
2.498ArgArg: 2.498 ± 0.571
2.732ArgSer: 2.732 ± 0.416
3.122ArgThr: 3.122 ± 0.595
2.42ArgVal: 2.42 ± 0.435
0.468ArgTrp: 0.468 ± 0.166
2.107ArgTyr: 2.107 ± 0.442
0.0ArgXaa: 0.0 ± 0.0
Ser
4.293SerAla: 4.293 ± 1.047
0.156SerCys: 0.156 ± 0.102
3.825SerAsp: 3.825 ± 0.615
4.605SerGlu: 4.605 ± 0.536
1.795SerPhe: 1.795 ± 0.392
4.761SerGly: 4.761 ± 0.678
1.405SerHis: 1.405 ± 0.4
3.668SerIle: 3.668 ± 0.638
4.605SerLys: 4.605 ± 0.607
5.308SerLeu: 5.308 ± 0.645
1.171SerMet: 1.171 ± 0.345
3.434SerAsn: 3.434 ± 0.638
1.405SerPro: 1.405 ± 0.307
2.576SerGln: 2.576 ± 0.378
3.278SerArg: 3.278 ± 0.633
3.746SerSer: 3.746 ± 0.696
3.59SerThr: 3.59 ± 0.616
3.356SerVal: 3.356 ± 0.773
1.015SerTrp: 1.015 ± 0.381
2.576SerTyr: 2.576 ± 0.436
0.0SerXaa: 0.0 ± 0.0
Thr
4.215ThrAla: 4.215 ± 0.842
0.312ThrCys: 0.312 ± 0.147
3.903ThrAsp: 3.903 ± 0.543
4.605ThrGlu: 4.605 ± 0.536
2.966ThrPhe: 2.966 ± 0.666
4.215ThrGly: 4.215 ± 0.642
1.327ThrHis: 1.327 ± 0.428
4.605ThrIle: 4.605 ± 0.532
4.761ThrLys: 4.761 ± 0.637
3.981ThrLeu: 3.981 ± 0.647
0.781ThrMet: 0.781 ± 0.239
3.668ThrAsn: 3.668 ± 0.459
1.483ThrPro: 1.483 ± 0.437
2.966ThrGln: 2.966 ± 0.672
1.639ThrArg: 1.639 ± 0.354
3.981ThrSer: 3.981 ± 0.608
4.839ThrThr: 4.839 ± 0.912
4.215ThrVal: 4.215 ± 0.671
0.702ThrTrp: 0.702 ± 0.252
2.81ThrTyr: 2.81 ± 0.498
0.0ThrXaa: 0.0 ± 0.0
Val
5.542ValAla: 5.542 ± 0.701
0.39ValCys: 0.39 ± 0.186
3.825ValAsp: 3.825 ± 0.559
5.854ValGlu: 5.854 ± 0.694
1.795ValPhe: 1.795 ± 0.346
4.683ValGly: 4.683 ± 0.698
0.702ValHis: 0.702 ± 0.259
4.293ValIle: 4.293 ± 0.735
5.308ValLys: 5.308 ± 0.551
4.293ValLeu: 4.293 ± 0.6
0.859ValMet: 0.859 ± 0.273
4.059ValAsn: 4.059 ± 0.65
1.717ValPro: 1.717 ± 0.333
1.873ValGln: 1.873 ± 0.426
2.185ValArg: 2.185 ± 0.383
4.527ValSer: 4.527 ± 0.566
4.371ValThr: 4.371 ± 0.67
4.917ValVal: 4.917 ± 0.728
0.624ValTrp: 0.624 ± 0.217
2.42ValTyr: 2.42 ± 0.453
0.0ValXaa: 0.0 ± 0.0
Trp
1.015TrpAla: 1.015 ± 0.315
0.156TrpCys: 0.156 ± 0.105
0.781TrpAsp: 0.781 ± 0.298
0.937TrpGlu: 0.937 ± 0.322
1.327TrpPhe: 1.327 ± 0.427
0.624TrpGly: 0.624 ± 0.202
0.0TrpHis: 0.0 ± 0.0
0.624TrpIle: 0.624 ± 0.251
1.327TrpLys: 1.327 ± 0.298
0.624TrpLeu: 0.624 ± 0.249
0.39TrpMet: 0.39 ± 0.162
0.937TrpAsn: 0.937 ± 0.291
0.078TrpPro: 0.078 ± 0.096
0.859TrpGln: 0.859 ± 0.335
0.156TrpArg: 0.156 ± 0.128
0.546TrpSer: 0.546 ± 0.199
0.937TrpThr: 0.937 ± 0.265
1.093TrpVal: 1.093 ± 0.257
0.156TrpTrp: 0.156 ± 0.09
0.781TrpTyr: 0.781 ± 0.472
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.795TyrAla: 1.795 ± 0.354
0.546TyrCys: 0.546 ± 0.241
2.498TyrAsp: 2.498 ± 0.406
2.42TyrGlu: 2.42 ± 0.386
2.107TyrPhe: 2.107 ± 0.452
2.342TyrGly: 2.342 ± 0.378
0.937TyrHis: 0.937 ± 0.229
2.498TyrIle: 2.498 ± 0.506
3.668TyrLys: 3.668 ± 0.641
3.2TyrLeu: 3.2 ± 0.511
0.937TyrMet: 0.937 ± 0.374
1.561TyrAsn: 1.561 ± 0.358
2.029TyrPro: 2.029 ± 0.518
2.654TyrGln: 2.654 ± 0.387
2.498TyrArg: 2.498 ± 0.556
2.264TyrSer: 2.264 ± 0.497
2.342TyrThr: 2.342 ± 0.431
2.732TyrVal: 2.732 ± 0.512
0.234TyrTrp: 0.234 ± 0.114
1.795TyrTyr: 1.795 ± 0.499
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 63 proteins (12813 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski