Shewanella phage S0112

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.2

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 63 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A482ME63|A0A482ME63_9CAUD Uncharacterized protein OS=Shewanella phage S0112 OX=2559821 GN=S0112_069 PE=4 SV=1
MM1 pKa = 7.65KK2 pKa = 10.04FNKK5 pKa = 9.78VEE7 pKa = 4.03VEE9 pKa = 3.85RR10 pKa = 11.84DD11 pKa = 3.03EE12 pKa = 4.15MGYY15 pKa = 10.49YY16 pKa = 10.07SHH18 pKa = 7.75PQLCEE23 pKa = 3.73CKK25 pKa = 10.49SIAEE29 pKa = 4.17CQEE32 pKa = 3.65YY33 pKa = 8.12LTFGDD38 pKa = 3.41VKK40 pKa = 10.6AWEE43 pKa = 4.24EE44 pKa = 3.65EE45 pKa = 4.18MEE47 pKa = 4.5FVFSYY52 pKa = 11.14TEE54 pKa = 3.78MEE56 pKa = 4.17KK57 pKa = 10.62EE58 pKa = 3.91VDD60 pKa = 3.86EE61 pKa = 4.57DD62 pKa = 3.83HH63 pKa = 7.15PAYY66 pKa = 9.87TSFFVEE72 pKa = 3.99GDD74 pKa = 3.28TDD76 pKa = 3.5ISAWEE81 pKa = 4.66PIPPTEE87 pKa = 4.93DD88 pKa = 3.56SILLSIHH95 pKa = 6.58ANEE98 pKa = 4.93EE99 pKa = 4.44GPSAWWASEE108 pKa = 3.96SS109 pKa = 3.6

Molecular weight: 12.67 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A482MEG3|A0A482MEG3_9CAUD DNA/RNA helicase OS=Shewanella phage S0112 OX=2559821 GN=S0112_005 PE=4 SV=1
MM1 pKa = 7.78LYY3 pKa = 8.97LTAFTVPLIYY13 pKa = 10.84VFLKK17 pKa = 10.51VFQQRR22 pKa = 11.84NIMAGEE28 pKa = 4.32LTRR31 pKa = 11.84VFLTSYY37 pKa = 10.44LITAFEE43 pKa = 4.91IISITLIATKK53 pKa = 10.04GWGIFIPLATGGAIGGVAAIRR74 pKa = 11.84FHH76 pKa = 4.68QWLRR80 pKa = 11.84KK81 pKa = 9.2RR82 pKa = 11.84DD83 pKa = 3.57ARR85 pKa = 11.84STKK88 pKa = 9.66IRR90 pKa = 3.55

Molecular weight: 10.18 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

63

0

63

15257

63

934

242.2

26.96

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.58 ± 0.381

1.213 ± 0.142

5.964 ± 0.208

6.62 ± 0.262

4.175 ± 0.175

7.131 ± 0.218

1.829 ± 0.202

6.508 ± 0.265

5.715 ± 0.37

8.619 ± 0.295

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.228 ± 0.176

4.431 ± 0.174

4.824 ± 0.27

3.893 ± 0.187

4.962 ± 0.212

6.168 ± 0.269

6.043 ± 0.339

6.187 ± 0.202

1.363 ± 0.147

3.546 ± 0.238
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski