Oricola thermophila

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Phyllobacteriaceae; Oricola

Average proteome isoelectric point is 6.24

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3798 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A6N1VBZ1|A0A6N1VBZ1_9RHIZ TRAP transporter substrate-binding protein OS=Oricola thermophila OX=2742145 GN=HTY61_00350 PE=3 SV=1
MM1 pKa = 7.64KK2 pKa = 10.21IKK4 pKa = 10.74SLLLGSAAALVAVSGARR21 pKa = 11.84AADD24 pKa = 3.45AVIIPEE30 pKa = 4.26PEE32 pKa = 3.59MVEE35 pKa = 4.04YY36 pKa = 11.19VRR38 pKa = 11.84VCDD41 pKa = 4.04AAGTGYY47 pKa = 10.49FYY49 pKa = 10.59IPGTEE54 pKa = 3.88TCLKK58 pKa = 9.35ISGFVRR64 pKa = 11.84YY65 pKa = 10.02QMDD68 pKa = 3.77FAGGDD73 pKa = 3.71GYY75 pKa = 11.49GDD77 pKa = 3.17QDD79 pKa = 3.54GWDD82 pKa = 3.44KK83 pKa = 11.3EE84 pKa = 4.22YY85 pKa = 11.33DD86 pKa = 3.62VNLLFEE92 pKa = 4.66AWNDD96 pKa = 3.58TEE98 pKa = 4.62YY99 pKa = 11.55GPLYY103 pKa = 10.18SQIRR107 pKa = 11.84LEE109 pKa = 4.15ADD111 pKa = 3.12GGVGEE116 pKa = 4.75DD117 pKa = 4.07ARR119 pKa = 11.84PLGDD123 pKa = 3.45SFVNIDD129 pKa = 3.13KK130 pKa = 10.96AYY132 pKa = 7.34FTLAGVTMGYY142 pKa = 10.54KK143 pKa = 10.32NSFWDD148 pKa = 3.55YY149 pKa = 11.28GFGGPADD156 pKa = 3.1IWNNGGPMTGMVGYY170 pKa = 7.21TANAGGFSISLALEE184 pKa = 4.61DD185 pKa = 5.51DD186 pKa = 5.03GSADD190 pKa = 3.39WTPAILGVVSGSIGDD205 pKa = 3.86LALTLGAVYY214 pKa = 10.22EE215 pKa = 4.52DD216 pKa = 4.86DD217 pKa = 4.07GATGEE222 pKa = 4.14VSDD225 pKa = 4.29WGFKK229 pKa = 8.63ATAAYY234 pKa = 10.79DD235 pKa = 3.34MGAAAVGFGVQYY247 pKa = 10.7SGEE250 pKa = 4.19TSGVNGSRR258 pKa = 11.84YY259 pKa = 5.24MTAYY263 pKa = 10.49DD264 pKa = 3.54WVLGADD270 pKa = 3.5VSFDD274 pKa = 3.36ATEE277 pKa = 4.04KK278 pKa = 10.96LSLGLGAQYY287 pKa = 10.93AINEE291 pKa = 4.3GGATDD296 pKa = 4.74DD297 pKa = 3.68DD298 pKa = 4.13WGIGAVASYY307 pKa = 10.73DD308 pKa = 3.38IVSGLNTEE316 pKa = 4.56LRR318 pKa = 11.84LRR320 pKa = 11.84WLEE323 pKa = 4.04DD324 pKa = 2.93TSTADD329 pKa = 4.65AVDD332 pKa = 3.56TSGWNARR339 pKa = 11.84LRR341 pKa = 11.84FTRR344 pKa = 11.84SFF346 pKa = 3.27

Molecular weight:
36.58 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A6N1VJK5|A0A6N1VJK5_9RHIZ TetR/AcrR family transcriptional regulator OS=Oricola thermophila OX=2742145 GN=HTY61_14655 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.27QPSKK9 pKa = 9.73LVRR12 pKa = 11.84KK13 pKa = 9.15RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.42GFRR19 pKa = 11.84ARR21 pKa = 11.84MATVGGRR28 pKa = 11.84KK29 pKa = 9.05VLAARR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 9.03RR41 pKa = 11.84LSAA44 pKa = 4.03

Molecular weight:
5.14 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3798

0

3798

1200695

41

3774

316.1

34.37

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.257 ± 0.052

0.845 ± 0.012

5.936 ± 0.036

6.383 ± 0.041

3.887 ± 0.026

8.69 ± 0.04

1.996 ± 0.018

5.506 ± 0.027

3.215 ± 0.033

9.539 ± 0.046

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.669 ± 0.019

2.674 ± 0.021

4.979 ± 0.029

2.645 ± 0.022

7.255 ± 0.048

5.271 ± 0.032

5.179 ± 0.026

7.595 ± 0.035

1.267 ± 0.018

2.211 ± 0.019

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski