Amino acid dipepetide frequency for Lactococcus virus bIL170

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
0.701AlaAla: 0.701 ± 0.277
0.2AlaCys: 0.2 ± 0.17
3.203AlaAsp: 3.203 ± 0.611
5.506AlaGlu: 5.506 ± 1.009
3.403AlaPhe: 3.403 ± 0.653
4.304AlaGly: 4.304 ± 0.722
0.901AlaHis: 0.901 ± 0.227
4.605AlaIle: 4.605 ± 0.889
5.405AlaLys: 5.405 ± 0.805
6.707AlaLeu: 6.707 ± 0.979
2.102AlaMet: 2.102 ± 0.633
4.505AlaAsn: 4.505 ± 0.812
0.701AlaPro: 0.701 ± 0.323
3.003AlaGln: 3.003 ± 0.699
1.902AlaArg: 1.902 ± 0.375
3.704AlaSer: 3.704 ± 0.682
3.904AlaThr: 3.904 ± 0.776
4.304AlaVal: 4.304 ± 0.964
1.602AlaTrp: 1.602 ± 0.514
1.702AlaTyr: 1.702 ± 0.4
0.0AlaXaa: 0.0 ± 0.0
Cys
0.3CysAla: 0.3 ± 0.18
0.1CysCys: 0.1 ± 0.103
0.2CysAsp: 0.2 ± 0.13
0.3CysGlu: 0.3 ± 0.183
0.0CysPhe: 0.0 ± 0.0
0.701CysGly: 0.701 ± 0.345
0.1CysHis: 0.1 ± 0.091
0.501CysIle: 0.501 ± 0.228
0.701CysLys: 0.701 ± 0.333
0.3CysLeu: 0.3 ± 0.196
0.1CysMet: 0.1 ± 0.123
0.501CysAsn: 0.501 ± 0.26
0.2CysPro: 0.2 ± 0.152
0.2CysGln: 0.2 ± 0.136
0.501CysArg: 0.501 ± 0.231
0.4CysSer: 0.4 ± 0.189
0.3CysThr: 0.3 ± 0.182
0.3CysVal: 0.3 ± 0.171
0.0CysTrp: 0.0 ± 0.0
0.501CysTyr: 0.501 ± 0.241
0.0CysXaa: 0.0 ± 0.0
Asp
1.902AspAla: 1.902 ± 0.49
0.1AspCys: 0.1 ± 0.108
3.203AspAsp: 3.203 ± 0.677
3.704AspGlu: 3.704 ± 0.732
3.303AspPhe: 3.303 ± 0.59
4.304AspGly: 4.304 ± 0.803
0.701AspHis: 0.701 ± 0.269
3.904AspIle: 3.904 ± 0.669
5.205AspLys: 5.205 ± 0.634
6.607AspLeu: 6.607 ± 0.774
1.101AspMet: 1.101 ± 0.221
3.704AspAsn: 3.704 ± 0.587
1.201AspPro: 1.201 ± 0.377
0.501AspGln: 0.501 ± 0.222
2.002AspArg: 2.002 ± 0.456
2.503AspSer: 2.503 ± 0.475
4.505AspThr: 4.505 ± 0.799
3.303AspVal: 3.303 ± 0.561
0.901AspTrp: 0.901 ± 0.298
3.003AspTyr: 3.003 ± 0.543
0.0AspXaa: 0.0 ± 0.0
Glu
3.804GluAla: 3.804 ± 0.69
0.4GluCys: 0.4 ± 0.219
3.403GluAsp: 3.403 ± 0.601
5.906GluGlu: 5.906 ± 1.183
4.304GluPhe: 4.304 ± 0.595
1.802GluGly: 1.802 ± 0.356
1.101GluHis: 1.101 ± 0.366
5.706GluIle: 5.706 ± 0.873
6.306GluLys: 6.306 ± 1.206
9.81GluLeu: 9.81 ± 1.505
2.603GluMet: 2.603 ± 0.565
5.405GluAsn: 5.405 ± 0.695
1.502GluPro: 1.502 ± 0.426
4.204GluGln: 4.204 ± 0.706
2.703GluArg: 2.703 ± 0.536
4.204GluSer: 4.204 ± 0.51
4.505GluThr: 4.505 ± 0.744
4.805GluVal: 4.805 ± 0.783
0.901GluTrp: 0.901 ± 0.307
2.803GluTyr: 2.803 ± 0.684
0.0GluXaa: 0.0 ± 0.0
Phe
3.403PheAla: 3.403 ± 0.734
0.4PheCys: 0.4 ± 0.217
3.303PheAsp: 3.303 ± 0.514
2.503PheGlu: 2.503 ± 0.577
1.802PhePhe: 1.802 ± 0.729
2.202PheGly: 2.202 ± 0.54
0.501PheHis: 0.501 ± 0.225
3.403PheIle: 3.403 ± 0.529
4.404PheLys: 4.404 ± 0.628
2.202PheLeu: 2.202 ± 0.449
0.801PheMet: 0.801 ± 0.246
2.703PheAsn: 2.703 ± 0.941
0.701PhePro: 0.701 ± 0.264
1.001PheGln: 1.001 ± 0.266
1.001PheArg: 1.001 ± 0.312
3.604PheSer: 3.604 ± 0.709
2.803PheThr: 2.803 ± 0.455
2.603PheVal: 2.603 ± 0.397
0.2PheTrp: 0.2 ± 0.143
1.602PheTyr: 1.602 ± 0.434
0.0PheXaa: 0.0 ± 0.0
Gly
3.604GlyAla: 3.604 ± 1.125
0.4GlyCys: 0.4 ± 0.19
2.803GlyAsp: 2.803 ± 0.628
4.304GlyGlu: 4.304 ± 0.651
2.302GlyPhe: 2.302 ± 0.494
3.604GlyGly: 3.604 ± 0.662
0.801GlyHis: 0.801 ± 0.256
4.404GlyIle: 4.404 ± 1.146
6.707GlyLys: 6.707 ± 0.782
4.805GlyLeu: 4.805 ± 0.918
1.201GlyMet: 1.201 ± 0.433
4.004GlyAsn: 4.004 ± 0.683
0.3GlyPro: 0.3 ± 0.157
1.802GlyGln: 1.802 ± 0.404
1.802GlyArg: 1.802 ± 0.419
4.705GlySer: 4.705 ± 0.767
3.804GlyThr: 3.804 ± 1.001
5.606GlyVal: 5.606 ± 1.151
1.201GlyTrp: 1.201 ± 0.305
3.103GlyTyr: 3.103 ± 0.644
0.0GlyXaa: 0.0 ± 0.0
His
0.801HisAla: 0.801 ± 0.232
0.4HisCys: 0.4 ± 0.283
0.701HisAsp: 0.701 ± 0.256
0.4HisGlu: 0.4 ± 0.207
0.501HisPhe: 0.501 ± 0.239
1.401HisGly: 1.401 ± 0.36
0.0HisHis: 0.0 ± 0.0
0.801HisIle: 0.801 ± 0.252
1.301HisLys: 1.301 ± 0.353
0.801HisLeu: 0.801 ± 0.324
0.0HisMet: 0.0 ± 0.0
1.201HisAsn: 1.201 ± 0.414
0.3HisPro: 0.3 ± 0.163
0.4HisGln: 0.4 ± 0.216
0.2HisArg: 0.2 ± 0.158
0.501HisSer: 0.501 ± 0.25
0.901HisThr: 0.901 ± 0.3
1.001HisVal: 1.001 ± 0.408
0.1HisTrp: 0.1 ± 0.102
0.701HisTyr: 0.701 ± 0.341
0.0HisXaa: 0.0 ± 0.0
Ile
5.205IleAla: 5.205 ± 0.617
0.0IleCys: 0.0 ± 0.0
4.705IleAsp: 4.705 ± 0.576
6.507IleGlu: 6.507 ± 0.921
2.402IlePhe: 2.402 ± 0.568
3.704IleGly: 3.704 ± 0.84
1.101IleHis: 1.101 ± 0.325
5.105IleIle: 5.105 ± 0.809
7.508IleLys: 7.508 ± 0.867
5.305IleLeu: 5.305 ± 1.001
1.301IleMet: 1.301 ± 0.415
5.205IleAsn: 5.205 ± 0.519
1.702IlePro: 1.702 ± 0.352
2.202IleGln: 2.202 ± 0.436
1.502IleArg: 1.502 ± 0.377
3.103IleSer: 3.103 ± 0.585
5.405IleThr: 5.405 ± 0.731
4.605IleVal: 4.605 ± 0.715
1.301IleTrp: 1.301 ± 0.462
2.503IleTyr: 2.503 ± 0.46
0.0IleXaa: 0.0 ± 0.0
Lys
6.607LysAla: 6.607 ± 0.968
0.601LysCys: 0.601 ± 0.324
4.605LysAsp: 4.605 ± 0.506
8.709LysGlu: 8.709 ± 1.477
2.503LysPhe: 2.503 ± 0.517
5.906LysGly: 5.906 ± 0.829
1.301LysHis: 1.301 ± 0.514
6.006LysIle: 6.006 ± 0.761
8.909LysLys: 8.909 ± 1.217
7.608LysLeu: 7.608 ± 0.908
2.903LysMet: 2.903 ± 0.508
6.006LysAsn: 6.006 ± 0.77
1.502LysPro: 1.502 ± 0.443
3.504LysGln: 3.504 ± 0.6
3.804LysArg: 3.804 ± 0.713
4.805LysSer: 4.805 ± 0.749
5.305LysThr: 5.305 ± 0.846
6.206LysVal: 6.206 ± 0.789
1.602LysTrp: 1.602 ± 0.381
4.004LysTyr: 4.004 ± 0.708
0.0LysXaa: 0.0 ± 0.0
Leu
6.006LeuAla: 6.006 ± 0.741
0.3LeuCys: 0.3 ± 0.157
4.805LeuAsp: 4.805 ± 0.646
6.006LeuGlu: 6.006 ± 0.868
3.003LeuPhe: 3.003 ± 0.605
4.605LeuGly: 4.605 ± 0.801
1.201LeuHis: 1.201 ± 0.414
7.107LeuIle: 7.107 ± 0.964
8.008LeuLys: 8.008 ± 0.86
6.507LeuLeu: 6.507 ± 1.034
1.802LeuMet: 1.802 ± 0.479
5.205LeuAsn: 5.205 ± 0.964
2.903LeuPro: 2.903 ± 0.617
3.704LeuGln: 3.704 ± 0.514
2.903LeuArg: 2.903 ± 0.653
5.105LeuSer: 5.105 ± 0.765
5.806LeuThr: 5.806 ± 0.749
5.906LeuVal: 5.906 ± 0.611
1.301LeuTrp: 1.301 ± 0.396
4.104LeuTyr: 4.104 ± 0.752
0.0LeuXaa: 0.0 ± 0.0
Met
2.202MetAla: 2.202 ± 0.489
0.1MetCys: 0.1 ± 0.093
1.602MetAsp: 1.602 ± 0.406
1.602MetGlu: 1.602 ± 0.52
0.601MetPhe: 0.601 ± 0.306
0.901MetGly: 0.901 ± 0.338
0.3MetHis: 0.3 ± 0.199
2.302MetIle: 2.302 ± 0.594
3.003MetLys: 3.003 ± 0.526
1.401MetLeu: 1.401 ± 0.421
0.3MetMet: 0.3 ± 0.177
1.902MetAsn: 1.902 ± 0.45
0.501MetPro: 0.501 ± 0.254
1.602MetGln: 1.602 ± 0.348
0.701MetArg: 0.701 ± 0.265
1.802MetSer: 1.802 ± 0.395
1.602MetThr: 1.602 ± 0.399
1.502MetVal: 1.502 ± 0.322
0.3MetTrp: 0.3 ± 0.172
1.401MetTyr: 1.401 ± 0.405
0.0MetXaa: 0.0 ± 0.0
Asn
5.205AsnAla: 5.205 ± 1.063
0.1AsnCys: 0.1 ± 0.107
4.004AsnAsp: 4.004 ± 0.642
4.605AsnGlu: 4.605 ± 0.68
2.302AsnPhe: 2.302 ± 0.53
6.507AsnGly: 6.507 ± 0.674
0.701AsnHis: 0.701 ± 0.241
4.505AsnIle: 4.505 ± 0.688
6.507AsnLys: 6.507 ± 0.826
6.006AsnLeu: 6.006 ± 0.771
1.502AsnMet: 1.502 ± 0.373
3.804AsnAsn: 3.804 ± 0.877
2.102AsnPro: 2.102 ± 0.443
2.002AsnGln: 2.002 ± 0.499
1.902AsnArg: 1.902 ± 0.383
5.506AsnSer: 5.506 ± 0.7
3.604AsnThr: 3.604 ± 0.665
3.303AsnVal: 3.303 ± 0.529
0.701AsnTrp: 0.701 ± 0.345
2.402AsnTyr: 2.402 ± 0.629
0.0AsnXaa: 0.0 ± 0.0
Pro
1.201ProAla: 1.201 ± 0.348
0.2ProCys: 0.2 ± 0.154
1.502ProAsp: 1.502 ± 0.426
1.502ProGlu: 1.502 ± 0.333
1.001ProPhe: 1.001 ± 0.309
0.3ProGly: 0.3 ± 0.166
0.0ProHis: 0.0 ± 0.0
1.902ProIle: 1.902 ± 0.47
2.002ProLys: 2.002 ± 0.519
1.802ProLeu: 1.802 ± 0.373
0.601ProMet: 0.601 ± 0.246
2.202ProAsn: 2.202 ± 0.645
0.701ProPro: 0.701 ± 0.263
1.101ProGln: 1.101 ± 0.33
0.601ProArg: 0.601 ± 0.29
0.801ProSer: 0.801 ± 0.251
2.202ProThr: 2.202 ± 0.425
1.702ProVal: 1.702 ± 0.534
0.2ProTrp: 0.2 ± 0.145
0.701ProTyr: 0.701 ± 0.253
0.0ProXaa: 0.0 ± 0.0
Gln
3.604GlnAla: 3.604 ± 0.733
0.1GlnCys: 0.1 ± 0.118
2.102GlnAsp: 2.102 ± 0.519
2.703GlnGlu: 2.703 ± 0.678
1.101GlnPhe: 1.101 ± 0.281
2.402GlnGly: 2.402 ± 0.607
0.3GlnHis: 0.3 ± 0.132
1.401GlnIle: 1.401 ± 0.252
2.302GlnLys: 2.302 ± 0.571
3.604GlnLeu: 3.604 ± 0.61
1.301GlnMet: 1.301 ± 0.324
2.402GlnAsn: 2.402 ± 0.484
1.101GlnPro: 1.101 ± 0.325
1.702GlnGln: 1.702 ± 0.431
1.301GlnArg: 1.301 ± 0.341
2.302GlnSer: 2.302 ± 0.385
2.202GlnThr: 2.202 ± 0.431
2.202GlnVal: 2.202 ± 0.459
0.601GlnTrp: 0.601 ± 0.218
1.502GlnTyr: 1.502 ± 0.365
0.0GlnXaa: 0.0 ± 0.0
Arg
2.603ArgAla: 2.603 ± 0.627
0.501ArgCys: 0.501 ± 0.214
1.301ArgAsp: 1.301 ± 0.423
2.503ArgGlu: 2.503 ± 0.564
0.601ArgPhe: 0.601 ± 0.24
1.401ArgGly: 1.401 ± 0.442
0.501ArgHis: 0.501 ± 0.195
2.302ArgIle: 2.302 ± 0.575
4.705ArgLys: 4.705 ± 0.88
3.303ArgLeu: 3.303 ± 0.609
0.901ArgMet: 0.901 ± 0.375
1.802ArgAsn: 1.802 ± 0.494
0.601ArgPro: 0.601 ± 0.233
1.502ArgGln: 1.502 ± 0.353
2.002ArgArg: 2.002 ± 0.45
2.002ArgSer: 2.002 ± 0.538
1.902ArgThr: 1.902 ± 0.39
1.602ArgVal: 1.602 ± 0.381
0.3ArgTrp: 0.3 ± 0.157
2.102ArgTyr: 2.102 ± 0.494
0.0ArgXaa: 0.0 ± 0.0
Ser
5.305SerAla: 5.305 ± 1.388
0.901SerCys: 0.901 ± 0.365
3.504SerAsp: 3.504 ± 0.698
3.804SerGlu: 3.804 ± 0.57
2.703SerPhe: 2.703 ± 0.441
5.806SerGly: 5.806 ± 1.27
1.001SerHis: 1.001 ± 0.284
3.704SerIle: 3.704 ± 0.606
5.005SerLys: 5.005 ± 0.847
5.506SerLeu: 5.506 ± 1.03
2.202SerMet: 2.202 ± 0.407
4.104SerAsn: 4.104 ± 0.752
1.301SerPro: 1.301 ± 0.402
1.301SerGln: 1.301 ± 0.325
2.402SerArg: 2.402 ± 0.365
5.205SerSer: 5.205 ± 0.981
3.403SerThr: 3.403 ± 0.511
4.104SerVal: 4.104 ± 0.793
0.801SerTrp: 0.801 ± 0.367
2.302SerTyr: 2.302 ± 0.519
0.0SerXaa: 0.0 ± 0.0
Thr
4.905ThrAla: 4.905 ± 0.684
0.2ThrCys: 0.2 ± 0.131
3.604ThrAsp: 3.604 ± 0.612
5.506ThrGlu: 5.506 ± 0.71
2.903ThrPhe: 2.903 ± 0.533
4.605ThrGly: 4.605 ± 0.519
0.4ThrHis: 0.4 ± 0.203
4.304ThrIle: 4.304 ± 0.805
4.304ThrLys: 4.304 ± 0.642
5.405ThrLeu: 5.405 ± 0.677
1.301ThrMet: 1.301 ± 0.349
4.104ThrAsn: 4.104 ± 0.668
1.902ThrPro: 1.902 ± 0.374
2.603ThrGln: 2.603 ± 0.561
2.102ThrArg: 2.102 ± 0.47
4.404ThrSer: 4.404 ± 0.633
4.204ThrThr: 4.204 ± 0.684
4.805ThrVal: 4.805 ± 0.838
0.801ThrTrp: 0.801 ± 0.311
2.202ThrTyr: 2.202 ± 0.493
0.0ThrXaa: 0.0 ± 0.0
Val
3.203ValAla: 3.203 ± 0.564
0.4ValCys: 0.4 ± 0.212
4.404ValAsp: 4.404 ± 0.689
5.205ValGlu: 5.205 ± 0.638
3.403ValPhe: 3.403 ± 0.633
3.504ValGly: 3.504 ± 0.501
0.701ValHis: 0.701 ± 0.241
4.304ValIle: 4.304 ± 0.567
6.006ValLys: 6.006 ± 0.786
3.804ValLeu: 3.804 ± 0.579
2.202ValMet: 2.202 ± 0.452
3.103ValAsn: 3.103 ± 0.549
1.902ValPro: 1.902 ± 0.361
1.802ValGln: 1.802 ± 0.444
3.203ValArg: 3.203 ± 0.652
6.707ValSer: 6.707 ± 1.309
4.705ValThr: 4.705 ± 0.676
3.704ValVal: 3.704 ± 0.738
0.701ValTrp: 0.701 ± 0.234
2.102ValTyr: 2.102 ± 0.387
0.0ValXaa: 0.0 ± 0.0
Trp
0.501TrpAla: 0.501 ± 0.261
0.2TrpCys: 0.2 ± 0.137
0.801TrpAsp: 0.801 ± 0.311
0.701TrpGlu: 0.701 ± 0.256
0.901TrpPhe: 0.901 ± 0.361
0.701TrpGly: 0.701 ± 0.348
0.1TrpHis: 0.1 ± 0.106
0.601TrpIle: 0.601 ± 0.217
1.101TrpLys: 1.101 ± 0.325
1.301TrpLeu: 1.301 ± 0.419
0.3TrpMet: 0.3 ± 0.151
2.102TrpAsn: 2.102 ± 0.47
0.0TrpPro: 0.0 ± 0.0
0.701TrpGln: 0.701 ± 0.273
0.4TrpArg: 0.4 ± 0.277
1.301TrpSer: 1.301 ± 0.324
0.701TrpThr: 0.701 ± 0.307
0.701TrpVal: 0.701 ± 0.305
0.0TrpTrp: 0.0 ± 0.0
0.901TrpTyr: 0.901 ± 0.279
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.301TyrAla: 1.301 ± 0.403
0.601TyrCys: 0.601 ± 0.358
1.902TyrAsp: 1.902 ± 0.612
4.204TyrGlu: 4.204 ± 0.666
2.102TyrPhe: 2.102 ± 0.396
2.603TyrGly: 2.603 ± 0.512
0.701TyrHis: 0.701 ± 0.263
3.504TyrIle: 3.504 ± 0.684
3.003TyrLys: 3.003 ± 0.798
3.504TyrLeu: 3.504 ± 0.706
1.001TyrMet: 1.001 ± 0.42
3.303TyrAsn: 3.303 ± 0.494
1.101TyrPro: 1.101 ± 0.33
1.401TyrGln: 1.401 ± 0.493
1.502TyrArg: 1.502 ± 0.466
1.802TyrSer: 1.802 ± 0.354
2.803TyrThr: 2.803 ± 0.531
2.803TyrVal: 2.803 ± 0.565
0.501TyrTrp: 0.501 ± 0.223
2.302TyrTyr: 2.302 ± 0.509
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 64 proteins (9991 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski