Shewanella phage Spp001

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Chaseviridae; Nefertitivirinae; Yushanvirus; Shewanella virus Spp001

Average proteome isoelectric point is 6.42

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 67 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|W6E9J9|W6E9J9_9CAUD Peptidase m15a OS=Shewanella phage Spp001 OX=1445859 GN=Spp001_63 PE=4 SV=1
MM1 pKa = 7.46SFNPITLKK9 pKa = 10.73ASRR12 pKa = 11.84SVYY15 pKa = 10.4TNADD19 pKa = 2.78WSILVQAVTNTAAQGVMSEE38 pKa = 4.58EE39 pKa = 4.38ALCSIPTPAQDD50 pKa = 3.34HH51 pKa = 6.67CDD53 pKa = 3.73SLRR56 pKa = 11.84GAMMLLATRR65 pKa = 11.84EE66 pKa = 4.11NFEE69 pKa = 3.94QFQIEE74 pKa = 4.11ALFNEE79 pKa = 4.71VAFLITGLTPSEE91 pKa = 4.39LDD93 pKa = 4.05EE94 pKa = 4.36VWADD98 pKa = 3.41ANEE101 pKa = 4.09EE102 pKa = 4.16FF103 pKa = 5.15

Molecular weight: 11.37 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|W6E894|W6E894_9CAUD Uncharacterized protein OS=Shewanella phage Spp001 OX=1445859 GN=Spp001_45 PE=4 SV=1
MM1 pKa = 7.59SSHH4 pKa = 7.54PIIVLATALLVFVGYY19 pKa = 9.36MLMTLTHH26 pKa = 7.19RR27 pKa = 11.84YY28 pKa = 9.3LNPKK32 pKa = 7.84FTRR35 pKa = 11.84GQNVRR40 pKa = 11.84IIAPIAEE47 pKa = 4.34LRR49 pKa = 11.84RR50 pKa = 11.84ALEE53 pKa = 4.34DD54 pKa = 3.49AMPMNSRR61 pKa = 11.84YY62 pKa = 9.66PSTLDD67 pKa = 2.88WAKK70 pKa = 10.61VRR72 pKa = 11.84TGVCGKK78 pKa = 10.01VLEE81 pKa = 4.65CGVRR85 pKa = 11.84HH86 pKa = 5.83VVIQVYY92 pKa = 8.96GRR94 pKa = 11.84RR95 pKa = 11.84VRR97 pKa = 11.84LPLDD101 pKa = 3.42SVKK104 pKa = 11.0

Molecular weight: 11.78 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

67

0

67

16574

41

1190

247.4

27.44

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.684 ± 0.439

1.122 ± 0.116

5.84 ± 0.235

5.985 ± 0.338

3.361 ± 0.198

6.89 ± 0.341

2.407 ± 0.135

5.352 ± 0.225

5.165 ± 0.232

8.073 ± 0.314

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.498 ± 0.173

4.091 ± 0.144

4.616 ± 0.199

4.127 ± 0.178

5.768 ± 0.205

5.943 ± 0.271

6.957 ± 0.331

7.343 ± 0.297

1.539 ± 0.178

3.24 ± 0.126
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski