Faeces associated gemycircularvirus 12

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Genomoviridae; Gemytondvirus; Gemytondvirus ostri1; Ostrich associated gemytondvirus 1

Average proteome isoelectric point is 8.19

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|T1YRW7|T1YRW7_9VIRU Replication associated protein OS=Faeces associated gemycircularvirus 12 OX=1391027 PE=3 SV=1
MM1 pKa = 8.04PFRR4 pKa = 11.84FDD6 pKa = 3.25NKK8 pKa = 10.33RR9 pKa = 11.84VFLTYY14 pKa = 10.34SQVGQRR20 pKa = 11.84TLDD23 pKa = 3.54SIYY26 pKa = 11.23DD27 pKa = 3.65FLSTLKK33 pKa = 10.55DD34 pKa = 3.47GRR36 pKa = 11.84GEE38 pKa = 3.98LVAIEE43 pKa = 4.56YY44 pKa = 10.29IVVARR49 pKa = 11.84EE50 pKa = 3.54RR51 pKa = 11.84HH52 pKa = 5.47LDD54 pKa = 3.57GGFHH58 pKa = 5.88FHH60 pKa = 7.15AFILFRR66 pKa = 11.84EE67 pKa = 4.77RR68 pKa = 11.84FQSRR72 pKa = 11.84NQRR75 pKa = 11.84IFDD78 pKa = 3.75FDD80 pKa = 3.72GLHH83 pKa = 6.62PNIEE87 pKa = 4.43SVRR90 pKa = 11.84GEE92 pKa = 3.69RR93 pKa = 11.84NVANKK98 pKa = 8.38ITYY101 pKa = 7.06TKK103 pKa = 10.67KK104 pKa = 9.76DD105 pKa = 3.47GEE107 pKa = 4.42FKK109 pKa = 10.75EE110 pKa = 5.41HH111 pKa = 6.83GDD113 pKa = 3.66EE114 pKa = 4.01PTVAEE119 pKa = 4.28RR120 pKa = 11.84ANKK123 pKa = 7.67QQHH126 pKa = 5.59WLDD129 pKa = 5.09LIDD132 pKa = 4.53SSTDD136 pKa = 2.97AADD139 pKa = 4.01FMGKK143 pKa = 9.82AKK145 pKa = 10.34SVAPRR150 pKa = 11.84DD151 pKa = 3.62FVLQNDD157 pKa = 3.53KK158 pKa = 11.36FEE160 pKa = 4.44AFARR164 pKa = 11.84KK165 pKa = 9.49YY166 pKa = 8.37YY167 pKa = 10.98NNVATYY173 pKa = 9.11VSEE176 pKa = 4.15YY177 pKa = 10.38GPEE180 pKa = 4.13DD181 pKa = 3.28FVVPAEE187 pKa = 4.3CNDD190 pKa = 3.19WVRR193 pKa = 11.84DD194 pKa = 3.83VLNEE198 pKa = 3.8VCFLGTCYY206 pKa = 10.74SQIPNSNIVRR216 pKa = 11.84ILGTRR221 pKa = 11.84SSQMLGPHH229 pKa = 5.7RR230 pKa = 11.84RR231 pKa = 11.84RR232 pKa = 11.84RR233 pKa = 11.84TRR235 pKa = 11.84QTEE238 pKa = 3.81WARR241 pKa = 11.84CLGDD245 pKa = 3.65HH246 pKa = 7.43FYY248 pKa = 11.62NSTWFNLDD256 pKa = 3.07EE257 pKa = 4.4VDD259 pKa = 3.7PSKK262 pKa = 11.02KK263 pKa = 10.19YY264 pKa = 11.01AVFDD268 pKa = 5.09DD269 pKa = 3.12IRR271 pKa = 11.84LNEE274 pKa = 3.79KK275 pKa = 10.54SYY277 pKa = 10.51WSWKK281 pKa = 8.96PWFGGQKK288 pKa = 10.07EE289 pKa = 4.19FTVTDD294 pKa = 3.68KK295 pKa = 10.81FRR297 pKa = 11.84HH298 pKa = 5.28KK299 pKa = 10.47RR300 pKa = 11.84KK301 pKa = 10.21LMWGKK306 pKa = 9.69PIIWVCNPASDD317 pKa = 3.99PRR319 pKa = 11.84NIAGLDD325 pKa = 3.35SAKK328 pKa = 10.25FAEE331 pKa = 4.52SNPRR335 pKa = 11.84CARR338 pKa = 11.84RR339 pKa = 11.84AQEE342 pKa = 4.83IEE344 pKa = 5.16LILCNTPNPNGMFCEE359 pKa = 4.14DD360 pKa = 4.23VVCILYY366 pKa = 10.11IFVIHH371 pKa = 6.55RR372 pKa = 11.84VGPIKK377 pKa = 10.49PPSNYY382 pKa = 9.53IGG384 pKa = 3.42

Molecular weight: 44.74 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|T1YRW7|T1YRW7_9VIRU Replication associated protein OS=Faeces associated gemycircularvirus 12 OX=1391027 PE=3 SV=1
MM1 pKa = 6.72YY2 pKa = 10.3RR3 pKa = 11.84KK4 pKa = 9.79GGKK7 pKa = 8.33YY8 pKa = 9.63KK9 pKa = 10.14KK10 pKa = 9.77RR11 pKa = 11.84SYY13 pKa = 10.79KK14 pKa = 9.26RR15 pKa = 11.84RR16 pKa = 11.84KK17 pKa = 7.78YY18 pKa = 10.88NKK20 pKa = 8.72NKK22 pKa = 9.63VSKK25 pKa = 10.31KK26 pKa = 6.7VWKK29 pKa = 10.05ASKK32 pKa = 10.04KK33 pKa = 8.95AAKK36 pKa = 9.34AQIYY40 pKa = 10.07RR41 pKa = 11.84MSEE44 pKa = 3.81TKK46 pKa = 10.43VIHH49 pKa = 4.95STGYY53 pKa = 9.89SSYY56 pKa = 8.85ITAAEE61 pKa = 4.12SQFLCALHH69 pKa = 6.56PFYY72 pKa = 11.18FMTNHH77 pKa = 5.4SRR79 pKa = 11.84EE80 pKa = 4.08AGLVGNEE87 pKa = 3.05FDD89 pKa = 4.25LRR91 pKa = 11.84YY92 pKa = 10.06FEE94 pKa = 5.26LQGSLNFDD102 pKa = 3.9GFSTALMSDD111 pKa = 2.56VWLRR115 pKa = 11.84ISLIRR120 pKa = 11.84TPEE123 pKa = 3.79YY124 pKa = 9.62WPNDD128 pKa = 2.85AGTPVSSTIPTSTYY142 pKa = 11.06ALPLSAEE149 pKa = 3.86PWFSKK154 pKa = 10.58FNSNSVKK161 pKa = 10.27VLQTKK166 pKa = 7.63TIRR169 pKa = 11.84LPGAAYY175 pKa = 10.32VGNQTSTNRR184 pKa = 11.84IHH186 pKa = 7.25KK187 pKa = 9.51YY188 pKa = 10.43KK189 pKa = 10.55NFKK192 pKa = 10.11IKK194 pKa = 9.89FRR196 pKa = 11.84KK197 pKa = 9.63LKK199 pKa = 9.96GKK201 pKa = 8.21KK202 pKa = 8.56TIMSNYY208 pKa = 9.59DD209 pKa = 3.27EE210 pKa = 5.67NIVAGAGRR218 pKa = 11.84RR219 pKa = 11.84IRR221 pKa = 11.84QGQFYY226 pKa = 10.01IIIHH230 pKa = 5.32MASVNAVSGTTSTDD244 pKa = 2.55IVRR247 pKa = 11.84RR248 pKa = 11.84WLDD251 pKa = 3.03WTYY254 pKa = 11.82SMYY257 pKa = 11.2YY258 pKa = 10.34KK259 pKa = 10.69DD260 pKa = 3.2II261 pKa = 4.69

Molecular weight: 30.12 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

645

261

384

322.5

37.43

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.202 ± 0.404

1.55 ± 0.679

5.426 ± 1.373

4.806 ± 1.013

6.047 ± 0.843

5.736 ± 0.006

2.326 ± 0.238

6.047 ± 0.272

7.597 ± 1.598

5.891 ± 0.084

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.86 ± 0.478

5.736 ± 0.217

4.186 ± 0.652

2.946 ± 0.153

7.597 ± 0.63

7.287 ± 1.556

5.426 ± 1.078

6.047 ± 0.62

2.326 ± 0.016

4.961 ± 1.126
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski