Azospirillum sp. RU38E

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodospirillales; Azospirillaceae; Azospirillum; unclassified Azospirillum

Average proteome isoelectric point is 6.55

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5137 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A239J751|A0A239J751_9PROT HAMP domain-containing protein OS=Azospirillum sp. RU38E OX=1907313 GN=SAMN05880556_11870 PE=4 SV=1
MM1 pKa = 7.52GIVVTSQSVTGGNGLKK17 pKa = 10.71LSGTATGVVAVSYY30 pKa = 11.37VNGSLLVTEE39 pKa = 5.18GGVAVDD45 pKa = 3.95TYY47 pKa = 11.49GITLPLKK54 pKa = 10.24EE55 pKa = 4.33IDD57 pKa = 3.51ASAVSGMAVSLTADD71 pKa = 3.22VAGGIRR77 pKa = 11.84LIGTGLDD84 pKa = 3.45DD85 pKa = 3.95TLVGGSGNDD94 pKa = 3.62TLIGGAGADD103 pKa = 3.74SLTGGGGADD112 pKa = 4.95DD113 pKa = 5.09FIAYY117 pKa = 10.26NSGTQYY123 pKa = 10.06MPPADD128 pKa = 4.41VITDD132 pKa = 3.82FVVGDD137 pKa = 4.89AIQFGAPVTSIYY149 pKa = 11.02DD150 pKa = 3.46GVRR153 pKa = 11.84RR154 pKa = 11.84SAFDD158 pKa = 3.76GEE160 pKa = 4.33LQVAGNADD168 pKa = 3.3GTTYY172 pKa = 11.05LGLGVSSIVGYY183 pKa = 10.5RR184 pKa = 11.84YY185 pKa = 8.1TLSNSYY191 pKa = 9.91AASAFTIADD200 pKa = 3.64SATGRR205 pKa = 11.84VTLVSQASVTPTEE218 pKa = 4.45GADD221 pKa = 3.6LLTGTTGNDD230 pKa = 3.36SIAGLGGNDD239 pKa = 3.9TIIGLTGSDD248 pKa = 3.87TLDD251 pKa = 3.51GGAGDD256 pKa = 4.96DD257 pKa = 4.35VMRR260 pKa = 11.84QNFGGYY266 pKa = 9.59SILYY270 pKa = 9.35GGAGSDD276 pKa = 3.61TAEE279 pKa = 3.93ITYY282 pKa = 10.72DD283 pKa = 3.55PANGARR289 pKa = 11.84THH291 pKa = 6.83SIVAQFNEE299 pKa = 4.38EE300 pKa = 3.96EE301 pKa = 4.15MSGVRR306 pKa = 11.84INTYY310 pKa = 8.53YY311 pKa = 10.75VSSAVEE317 pKa = 4.06DD318 pKa = 3.79EE319 pKa = 4.73VYY321 pKa = 10.96LIDD324 pKa = 4.46VEE326 pKa = 4.15RR327 pKa = 11.84AIINGVEE334 pKa = 4.02YY335 pKa = 10.44KK336 pKa = 10.35ISNGTLYY343 pKa = 11.29DD344 pKa = 3.68NVLTGAAEE352 pKa = 4.08VRR354 pKa = 11.84NLASGNRR361 pKa = 11.84GADD364 pKa = 3.54SITGGNLQDD373 pKa = 3.57TLYY376 pKa = 10.74GGWGGDD382 pKa = 3.58TLRR385 pKa = 11.84GGAGDD390 pKa = 4.13DD391 pKa = 3.75LLVGGRR397 pKa = 11.84DD398 pKa = 3.32SDD400 pKa = 4.58LLDD403 pKa = 4.1GGAGFDD409 pKa = 4.43RR410 pKa = 11.84AALDD414 pKa = 3.71YY415 pKa = 11.45SEE417 pKa = 4.87NAYY420 pKa = 10.37AVAITVTDD428 pKa = 3.62NNITIDD434 pKa = 4.49NGDD437 pKa = 3.76NDD439 pKa = 3.66QLISIEE445 pKa = 4.13EE446 pKa = 4.13VQVTGTRR453 pKa = 11.84YY454 pKa = 10.57ADD456 pKa = 3.88IITGGNRR463 pKa = 11.84SEE465 pKa = 4.3TLNGGAGADD474 pKa = 4.16LIKK477 pKa = 10.92ACGGADD483 pKa = 3.14ILNGGTGNDD492 pKa = 4.12TLWGGTGDD500 pKa = 4.18DD501 pKa = 4.23LFVLGGRR508 pKa = 11.84RR509 pKa = 11.84SIDD512 pKa = 3.26DD513 pKa = 3.51TDD515 pKa = 3.84YY516 pKa = 11.64VADD519 pKa = 4.51FHH521 pKa = 8.95AGDD524 pKa = 3.69QLSFTHH530 pKa = 6.9RR531 pKa = 11.84LEE533 pKa = 4.09ATEE536 pKa = 5.08LSITSYY542 pKa = 10.72QAGNGSGLQLGQIRR556 pKa = 11.84YY557 pKa = 8.55EE558 pKa = 3.99NLADD562 pKa = 3.7GMVRR566 pKa = 11.84LYY568 pKa = 11.09VGADD572 pKa = 3.4DD573 pKa = 5.9VEE575 pKa = 4.69GADD578 pKa = 3.66YY579 pKa = 10.34TVDD582 pKa = 3.08IAGLGTGALLSISGGNRR599 pKa = 11.84LVLAPTAITNGQGTANADD617 pKa = 3.34IVRR620 pKa = 11.84GAAIDD625 pKa = 3.52TLLIGGKK632 pKa = 10.23GNDD635 pKa = 3.65VIIGGSGTQAAGMEE649 pKa = 4.51ANFADD654 pKa = 4.04VTILRR659 pKa = 11.84DD660 pKa = 3.48ATGVVQVIDD669 pKa = 3.42NRR671 pKa = 11.84ANGEE675 pKa = 4.12GADD678 pKa = 3.66VLSGIEE684 pKa = 4.15LLRR687 pKa = 11.84LNDD690 pKa = 4.66RR691 pKa = 11.84IVLLTDD697 pKa = 3.94PDD699 pKa = 3.87LTSVVPSTTVDD710 pKa = 3.53EE711 pKa = 4.51DD712 pKa = 3.65WYY714 pKa = 10.96LAEE717 pKa = 4.79NPDD720 pKa = 3.54VADD723 pKa = 3.73VVAQGLLQSGAQHH736 pKa = 5.8FQLYY740 pKa = 8.87GQAEE744 pKa = 4.49GRR746 pKa = 11.84QPNAVSDD753 pKa = 3.77NLGNGFSEE761 pKa = 4.18SAYY764 pKa = 10.13LDD766 pKa = 3.46RR767 pKa = 11.84YY768 pKa = 9.93ADD770 pKa = 3.03VRR772 pKa = 11.84AAVNQGIFTSGYY784 pKa = 9.0HH785 pKa = 6.41HH786 pKa = 6.37YY787 pKa = 10.24QVAGRR792 pKa = 11.84AEE794 pKa = 4.02GRR796 pKa = 11.84AGYY799 pKa = 10.16VDD801 pKa = 3.91QGLLAYY807 pKa = 10.07GFDD810 pKa = 3.36EE811 pKa = 4.98SYY813 pKa = 11.58YY814 pKa = 10.8LAANPDD820 pKa = 3.12VAASVRR826 pKa = 11.84VGDD829 pKa = 3.75LASGYY834 pKa = 6.97EE835 pKa = 4.0HH836 pKa = 6.66YY837 pKa = 10.79VRR839 pKa = 11.84SGFAEE844 pKa = 4.18GRR846 pKa = 11.84DD847 pKa = 3.39ANAFFDD853 pKa = 4.11TDD855 pKa = 2.92WYY857 pKa = 10.54LAHH860 pKa = 6.65NPDD863 pKa = 2.62VAAAVQQGVLSSAAMHH879 pKa = 6.57YY880 pKa = 8.07YY881 pKa = 9.51TYY883 pKa = 10.76GWRR886 pKa = 11.84EE887 pKa = 3.57GRR889 pKa = 11.84DD890 pKa = 3.21PSAAFDD896 pKa = 3.68VSAYY900 pKa = 10.47AADD903 pKa = 4.09NPDD906 pKa = 3.01VAAAGINPLLHH917 pKa = 5.76YY918 pKa = 9.96LQYY921 pKa = 10.98GYY923 pKa = 11.24NEE925 pKa = 3.73GRR927 pKa = 11.84IVRR930 pKa = 11.84GLADD934 pKa = 3.81SDD936 pKa = 3.81WAA938 pKa = 4.5

Molecular weight: 96.63 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A239CR87|A0A239CR87_9PROT ATP synthase subunit a OS=Azospirillum sp. RU38E OX=1907313 GN=atpB PE=3 SV=1
MM1 pKa = 7.49KK2 pKa = 10.41RR3 pKa = 11.84PFQPSKK9 pKa = 9.2IVRR12 pKa = 11.84ARR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.49GFRR19 pKa = 11.84ARR21 pKa = 11.84MATVAGRR28 pKa = 11.84NIINKK33 pKa = 9.09RR34 pKa = 11.84RR35 pKa = 11.84AQGRR39 pKa = 11.84KK40 pKa = 9.09RR41 pKa = 11.84LSAA44 pKa = 3.93

Molecular weight: 5.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5137

0

5137

1820581

25

12860

354.4

38.22

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.858 ± 0.048

0.763 ± 0.011

5.807 ± 0.028

5.0 ± 0.036

3.382 ± 0.023

9.071 ± 0.05

1.961 ± 0.021

4.626 ± 0.022

2.918 ± 0.026

10.916 ± 0.045

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.324 ± 0.02

2.744 ± 0.037

5.52 ± 0.045

3.58 ± 0.025

7.048 ± 0.046

5.203 ± 0.051

5.525 ± 0.073

7.139 ± 0.03

1.382 ± 0.016

2.232 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski