Sinorhizobium phage phiM5

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Podoviridae; unclassified Podoviridae

Average proteome isoelectric point is 6.38

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 59 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1Y0SVR4|A0A1Y0SVR4_9CAUD Putative internal virion protein OS=Sinorhizobium phage phiM5 OX=2005789 PE=4 SV=1
MM1 pKa = 7.53SYY3 pKa = 11.29GRR5 pKa = 11.84GTYY8 pKa = 9.89GKK10 pKa = 9.69ALYY13 pKa = 9.73GIWSYY18 pKa = 11.48QDD20 pKa = 2.92SGAATTALTYY30 pKa = 10.9GSADD34 pKa = 3.04STMTLVSRR42 pKa = 11.84ADD44 pKa = 4.05PIEE47 pKa = 4.17ALTTIASIGTRR58 pKa = 11.84LTTGQAALTGAATLTADD75 pKa = 4.4SVVEE79 pKa = 4.53NINGAHH85 pKa = 6.35FSVSAALSAVSEE97 pKa = 4.13RR98 pKa = 11.84LQPYY102 pKa = 9.45AAALDD107 pKa = 4.27SVSSASAAPSVLVFGALQPIEE128 pKa = 4.55AASVMAPNATRR139 pKa = 11.84VTSAACALTALSTILANGRR158 pKa = 11.84GIYY161 pKa = 10.25DD162 pKa = 5.42PIPADD167 pKa = 3.44SGVWQVDD174 pKa = 4.11TPAEE178 pKa = 4.07NLWTPISEE186 pKa = 4.45NSTSWTEE193 pKa = 3.99TPVNGG198 pKa = 4.69

Molecular weight: 20.12 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1Y0SYA6|A0A1Y0SYA6_9CAUD Uncharacterized protein OS=Sinorhizobium phage phiM5 OX=2005789 PE=4 SV=1
MM1 pKa = 7.48SRR3 pKa = 11.84PDD5 pKa = 3.44LPKK8 pKa = 9.67QLRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84LRR15 pKa = 11.84QLADD19 pKa = 3.24DD20 pKa = 4.53TGCISYY26 pKa = 10.59EE27 pKa = 3.9VVRR30 pKa = 11.84QSKK33 pKa = 9.82HH34 pKa = 4.83AVIDD38 pKa = 3.94FTHH41 pKa = 7.15PSVHH45 pKa = 6.11HH46 pKa = 5.78PVRR49 pKa = 11.84LTMSFTASDD58 pKa = 3.37DD59 pKa = 3.51RR60 pKa = 11.84AYY62 pKa = 11.3SNQRR66 pKa = 11.84ADD68 pKa = 3.22LARR71 pKa = 11.84AIRR74 pKa = 11.84RR75 pKa = 11.84EE76 pKa = 3.91QAHH79 pKa = 4.79VV80 pKa = 3.09

Molecular weight: 9.28 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

59

0

59

13774

36

814

233.5

25.42

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.531 ± 0.419

0.646 ± 0.098

6.447 ± 0.233

5.91 ± 0.389

3.093 ± 0.163

7.812 ± 0.483

1.735 ± 0.154

4.458 ± 0.153

4.966 ± 0.297

8.581 ± 0.242

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.352 ± 0.159

3.645 ± 0.219

4.893 ± 0.37

3.695 ± 0.409

6.164 ± 0.29

5.714 ± 0.319

6.309 ± 0.345

6.955 ± 0.276

1.634 ± 0.176

2.461 ± 0.161
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski