Escherichia phage phAPEC8

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Phapecoctavirus; Escherichia virus phAPEC8

Average proteome isoelectric point is 5.64

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 269 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|K7QLQ5|K7QLQ5_9CAUD Uncharacterized protein OS=Escherichia phage phAPEC8 OX=1229753 GN=phAPEC8_0089 PE=4 SV=1
MM1 pKa = 7.8SYY3 pKa = 11.14RR4 pKa = 11.84NVEE7 pKa = 4.16EE8 pKa = 4.23LTEE11 pKa = 4.02KK12 pKa = 10.69AQAIMALVDD21 pKa = 3.83EE22 pKa = 4.74LTKK25 pKa = 10.45EE26 pKa = 4.1AEE28 pKa = 4.08DD29 pKa = 3.58AGFGLSLEE37 pKa = 4.63DD38 pKa = 4.98DD39 pKa = 5.12AICFEE44 pKa = 4.98DD45 pKa = 3.62WQSSSCYY52 pKa = 10.63GEE54 pKa = 4.59GDD56 pKa = 3.45DD57 pKa = 4.75GFVVNSQGVWHH68 pKa = 6.75SSSCC72 pKa = 3.78

Molecular weight: 7.9 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|K7QL48|K7QL48_9CAUD Uncharacterized protein OS=Escherichia phage phAPEC8 OX=1229753 GN=phAPEC8_0049 PE=4 SV=1
MM1 pKa = 7.7KK2 pKa = 10.2GNKK5 pKa = 8.74MKK7 pKa = 10.8VNQVPSAYY15 pKa = 8.96TLWLARR21 pKa = 11.84ALHH24 pKa = 4.77QTIKK28 pKa = 10.84NPKK31 pKa = 9.31KK32 pKa = 9.93PMKK35 pKa = 9.96SGKK38 pKa = 10.15KK39 pKa = 9.85FMSKK43 pKa = 10.17GKK45 pKa = 6.8TTFGFTRR52 pKa = 11.84MSVKK56 pKa = 9.65MKK58 pKa = 10.57RR59 pKa = 11.84LGYY62 pKa = 8.83KK63 pKa = 9.76PNYY66 pKa = 8.9ARR68 pKa = 11.84YY69 pKa = 7.96PSEE72 pKa = 4.42YY73 pKa = 10.36NWFDD77 pKa = 3.02

Molecular weight: 9.07 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

269

0

269

45072

33

1017

167.6

18.96

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.885 ± 0.169

1.462 ± 0.089

6.372 ± 0.129

7.546 ± 0.216

4.402 ± 0.107

6.55 ± 0.197

1.884 ± 0.096

6.208 ± 0.132

6.696 ± 0.21

8.032 ± 0.156

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.822 ± 0.091

5.371 ± 0.138

3.583 ± 0.116

3.124 ± 0.098

4.047 ± 0.129

5.886 ± 0.129

6.086 ± 0.243

6.967 ± 0.162

1.582 ± 0.086

4.497 ± 0.114
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski