Great Island virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Duplornaviricota; Resentoviricetes; Reovirales; Reoviridae; Sedoreovirinae; Orbivirus

Average proteome isoelectric point is 7.32

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 11 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|E1AA97|E1AA97_9REOV VP6(Hel) OS=Great Island virus OX=204269 PE=4 SV=1
MM1 pKa = 7.38SADD4 pKa = 3.33KK5 pKa = 10.29QIPMIRR11 pKa = 11.84KK12 pKa = 7.78PFTRR16 pKa = 11.84TIVLKK21 pKa = 10.09STGKK25 pKa = 10.16DD26 pKa = 2.94DD27 pKa = 5.03YY28 pKa = 10.44VAKK31 pKa = 9.79MCNALGCEE39 pKa = 4.24YY40 pKa = 10.82LVVRR44 pKa = 11.84HH45 pKa = 5.59GLGMHH50 pKa = 6.11VAGSNTPTHH59 pKa = 6.29GCMLLLIPGAGSFRR73 pKa = 11.84LIDD76 pKa = 3.8RR77 pKa = 11.84DD78 pKa = 3.11QSTFVVVSSDD88 pKa = 3.21GLEE91 pKa = 4.14VQQDD95 pKa = 3.32RR96 pKa = 11.84WPGMRR101 pKa = 11.84FEE103 pKa = 4.71AVDD106 pKa = 3.42MFPRR110 pKa = 11.84CCRR113 pKa = 11.84IVVGDD118 pKa = 3.64VAADD122 pKa = 3.39SEE124 pKa = 4.51IRR126 pKa = 11.84FGSASGTVPPYY137 pKa = 9.66TAGEE141 pKa = 4.14SMEE144 pKa = 4.26AQDD147 pKa = 5.21EE148 pKa = 4.34ISLPGLDD155 pKa = 4.49FSVPDD160 pKa = 3.4SDD162 pKa = 3.94LRR164 pKa = 11.84EE165 pKa = 3.99YY166 pKa = 10.75RR167 pKa = 11.84EE168 pKa = 3.72KK169 pKa = 10.9LRR171 pKa = 11.84EE172 pKa = 4.09EE173 pKa = 4.19KK174 pKa = 10.53EE175 pKa = 3.76EE176 pKa = 3.7RR177 pKa = 11.84AAAILSALRR186 pKa = 11.84TTQQSRR192 pKa = 11.84TLGRR196 pKa = 11.84LHH198 pKa = 6.71GVKK201 pKa = 9.91TSDD204 pKa = 3.92LVTLRR209 pKa = 11.84PPQPPTPGRR218 pKa = 11.84NGHH221 pKa = 5.63SALMSSAPVPPPLVPTSSSTGSPALSTTGLKK252 pKa = 8.9RR253 pKa = 11.84TQKK256 pKa = 10.33LAPPPVAEE264 pKa = 4.29SAGPTFGADD273 pKa = 2.84SNRR276 pKa = 11.84FIEE279 pKa = 4.44EE280 pKa = 3.5ALEE283 pKa = 5.08AVMQDD288 pKa = 3.74DD289 pKa = 3.92ASGRR293 pKa = 11.84LNFGGEE299 pKa = 4.17PEE301 pKa = 4.38SVGLFSQNLGSFDD314 pKa = 3.95IPPSQLPAYY323 pKa = 9.6EE324 pKa = 3.93IDD326 pKa = 3.45EE327 pKa = 4.27TRR329 pKa = 11.84NKK331 pKa = 9.79YY332 pKa = 10.46KK333 pKa = 10.57YY334 pKa = 10.36VGMRR338 pKa = 11.84TSTRR342 pKa = 11.84LHH344 pKa = 6.8ALACDD349 pKa = 3.37DD350 pKa = 3.28RR351 pKa = 11.84VYY353 pKa = 10.66FVPSAEE359 pKa = 3.95

Molecular weight: 38.88 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|E1AA99|E1AA99_9REOV NS3 OS=Great Island virus OX=204269 PE=4 SV=1
MM1 pKa = 7.39KK2 pKa = 10.32NEE4 pKa = 3.55KK5 pKa = 9.68AAYY8 pKa = 8.47GAASEE13 pKa = 4.25VLKK16 pKa = 10.88DD17 pKa = 4.17DD18 pKa = 3.45EE19 pKa = 4.57TTRR22 pKa = 11.84MLKK25 pKa = 8.71MQVNEE30 pKa = 4.22CSLTEE35 pKa = 3.85MRR37 pKa = 11.84QTYY40 pKa = 9.73QSLKK44 pKa = 9.35RR45 pKa = 11.84RR46 pKa = 11.84CRR48 pKa = 11.84LLYY51 pKa = 10.5YY52 pKa = 10.96GEE54 pKa = 4.6LLCLAAALGLTLVLMVPSASRR75 pKa = 11.84VLEE78 pKa = 4.31GALTQANITGHH89 pKa = 6.33VITGILTSLAIFLQHH104 pKa = 5.99HH105 pKa = 6.25RR106 pKa = 11.84ARR108 pKa = 11.84LLKK111 pKa = 10.05RR112 pKa = 11.84KK113 pKa = 9.72RR114 pKa = 11.84SIKK117 pKa = 9.65RR118 pKa = 11.84DD119 pKa = 2.86IVKK122 pKa = 10.4RR123 pKa = 11.84MTYY126 pKa = 9.85ISLARR131 pKa = 11.84RR132 pKa = 11.84MGSQFPEE139 pKa = 4.2SAGAGSDD146 pKa = 3.38FRR148 pKa = 11.84ARR150 pKa = 11.84LMALAEE156 pKa = 4.04EE157 pKa = 4.72AEE159 pKa = 4.29RR160 pKa = 11.84ARR162 pKa = 11.84DD163 pKa = 3.65DD164 pKa = 3.96SDD166 pKa = 2.75WRR168 pKa = 11.84RR169 pKa = 11.84WPP171 pKa = 3.41

Molecular weight: 19.4 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

11

0

11

5845

171

1285

531.4

59.92

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.393 ± 0.615

1.317 ± 0.203

5.8 ± 0.34

6.176 ± 0.344

3.49 ± 0.311

5.885 ± 0.387

2.789 ± 0.259

4.808 ± 0.302

2.515 ± 0.455

9.478 ± 0.349

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.652 ± 0.187

2.618 ± 0.266

5.287 ± 0.354

3.849 ± 0.329

10.197 ± 0.383

6.347 ± 0.377

5.954 ± 0.267

7.169 ± 0.224

1.112 ± 0.231

3.165 ± 0.239
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski