Streptomyces sp. JV178

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptomycetales; Streptomycetaceae; Streptomyces; unclassified Streptomyces

Average proteome isoelectric point is 6.32

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 8845 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2G9DZ85|A0A2G9DZ85_9ACTN Iron permease OS=Streptomyces sp. JV178 OX=858632 GN=CTU88_05865 PE=3 SV=1
MM1 pKa = 7.81TDD3 pKa = 3.42DD4 pKa = 3.32QHH6 pKa = 9.59GEE8 pKa = 4.04GTPQQGGQSGWEE20 pKa = 3.83RR21 pKa = 11.84LPQGDD26 pKa = 4.38YY27 pKa = 11.43DD28 pKa = 4.65DD29 pKa = 4.95GATTFVQLPEE39 pKa = 4.39GGIDD43 pKa = 3.5ALFAADD49 pKa = 4.81SPLAAPGHH57 pKa = 6.43GYY59 pKa = 9.88VPPPIAPNPPGAAGTDD75 pKa = 3.67PSGTGSWAMPAEE87 pKa = 4.77GAQWHH92 pKa = 7.19DD93 pKa = 4.05PNAGQQQPPAQDD105 pKa = 2.8GFTYY109 pKa = 10.76NPGSTGQWTFEE120 pKa = 3.99EE121 pKa = 5.0PGAQEE126 pKa = 4.04GTAPGHH132 pKa = 6.56DD133 pKa = 3.71VTGEE137 pKa = 3.58WSIPVAGGDD146 pKa = 3.84LPDD149 pKa = 3.75EE150 pKa = 4.63SGEE153 pKa = 4.14FSTSALVEE161 pKa = 4.16QWGGTPPATLPGGAPAPWATEE182 pKa = 4.01QIGTAWTAPPPPTRR196 pKa = 11.84AEE198 pKa = 3.72EE199 pKa = 4.26SAAGEE204 pKa = 4.16PPADD208 pKa = 3.9ARR210 pKa = 11.84AEE212 pKa = 4.2APGEE216 pKa = 3.93PSSAPVEE223 pKa = 4.39APAPDD228 pKa = 3.62ASEE231 pKa = 4.22EE232 pKa = 4.26TPEE235 pKa = 4.22APEE238 pKa = 4.42GAQEE242 pKa = 4.16SAGGDD247 pKa = 3.55EE248 pKa = 4.45QAAEE252 pKa = 4.26ADD254 pKa = 3.97GDD256 pKa = 4.14STGPQEE262 pKa = 4.58PGTPAEE268 pKa = 4.52ASDD271 pKa = 4.89AEE273 pKa = 4.5TPDD276 pKa = 4.63AEE278 pKa = 4.71DD279 pKa = 4.6AGAADD284 pKa = 4.52PAAEE288 pKa = 4.24PTPPPHH294 pKa = 7.68DD295 pKa = 3.93DD296 pKa = 4.16HH297 pKa = 7.13PLASYY302 pKa = 10.27VLSVNGADD310 pKa = 5.33RR311 pKa = 11.84PVTDD315 pKa = 3.02AWIGEE320 pKa = 4.15SLLYY324 pKa = 10.18VLRR327 pKa = 11.84EE328 pKa = 3.94RR329 pKa = 11.84LGLAGAKK336 pKa = 9.72DD337 pKa = 3.75GCSQGEE343 pKa = 4.12CGACNVQVDD352 pKa = 3.94GRR354 pKa = 11.84LVASCLVPAVTAASSEE370 pKa = 3.91VRR372 pKa = 11.84TVEE375 pKa = 3.85GLAVDD380 pKa = 4.62GQPSDD385 pKa = 3.61VQRR388 pKa = 11.84ALARR392 pKa = 11.84CGAVQCGFCVPGMAMTVHH410 pKa = 7.27DD411 pKa = 5.16LLEE414 pKa = 4.78GNPAPTEE421 pKa = 4.03LEE423 pKa = 3.87ARR425 pKa = 11.84QALCGNLCRR434 pKa = 11.84CSGYY438 pKa = 10.31RR439 pKa = 11.84GVLAAVRR446 pKa = 11.84DD447 pKa = 4.42VVAEE451 pKa = 4.45RR452 pKa = 11.84AAHH455 pKa = 6.06AAGDD459 pKa = 4.1GDD461 pKa = 4.0TGADD465 pKa = 3.26TDD467 pKa = 3.87EE468 pKa = 4.58ARR470 pKa = 11.84IPHH473 pKa = 5.5QAGPGAGGVNPAAYY487 pKa = 7.67EE488 pKa = 4.12AQGTPPQVPNDD499 pKa = 3.84PGQSYY504 pKa = 10.58GGQGLSFGGQDD515 pKa = 3.54DD516 pKa = 4.89TYY518 pKa = 11.01GGQGQSFGGHH528 pKa = 6.22GDD530 pKa = 3.47TYY532 pKa = 11.11GGQGQSFGGQDD543 pKa = 3.23QSFGGQGDD551 pKa = 4.19TYY553 pKa = 10.89GGQGQSFGGHH563 pKa = 6.72DD564 pKa = 4.24DD565 pKa = 3.75SYY567 pKa = 11.69GGQGQSFGGHH577 pKa = 6.22GDD579 pKa = 3.47TYY581 pKa = 11.11GGQGQSFGGQDD592 pKa = 3.27QSFGGQDD599 pKa = 3.21QSFGGQDD606 pKa = 3.29DD607 pKa = 4.86TYY609 pKa = 11.01GGQGQSFGGHH619 pKa = 7.18DD620 pKa = 3.96DD621 pKa = 3.78TYY623 pKa = 11.43GGQGRR628 pKa = 11.84NFGDD632 pKa = 3.32QDD634 pKa = 3.36QSFGQDD640 pKa = 2.59GGQAA644 pKa = 3.31

Molecular weight: 64.6 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2G9DQE3|A0A2G9DQE3_9ACTN Uncharacterized protein OS=Streptomyces sp. JV178 OX=858632 GN=CTU88_19325 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 8.7THH17 pKa = 5.15GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AILANRR35 pKa = 11.84RR36 pKa = 11.84AKK38 pKa = 10.46GRR40 pKa = 11.84ASLSAA45 pKa = 3.83

Molecular weight: 5.22 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

8845

0

8845

2953601

26

7399

333.9

35.79

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.31 ± 0.037

0.779 ± 0.008

6.164 ± 0.02

5.689 ± 0.028

2.729 ± 0.014

9.524 ± 0.03

2.328 ± 0.014

3.1 ± 0.019

2.199 ± 0.024

10.231 ± 0.034

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.689 ± 0.01

1.827 ± 0.015

6.144 ± 0.026

2.669 ± 0.016

8.031 ± 0.031

5.078 ± 0.021

6.358 ± 0.026

8.439 ± 0.025

1.573 ± 0.011

2.138 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski