Amino acid dipepetide frequency for Escherichia phage C1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
9.045AlaAla: 9.045 ± 1.743
1.029AlaCys: 1.029 ± 0.31
4.412AlaAsp: 4.412 ± 0.549
6.324AlaGlu: 6.324 ± 0.799
3.015AlaPhe: 3.015 ± 0.513
6.986AlaGly: 6.986 ± 0.865
0.809AlaHis: 0.809 ± 0.299
6.03AlaIle: 6.03 ± 0.773
6.25AlaLys: 6.25 ± 0.744
7.942AlaLeu: 7.942 ± 0.817
4.191AlaMet: 4.191 ± 0.663
4.118AlaAsn: 4.118 ± 0.705
2.206AlaPro: 2.206 ± 0.354
3.603AlaGln: 3.603 ± 0.674
5.515AlaArg: 5.515 ± 0.617
5.147AlaSer: 5.147 ± 0.703
4.927AlaThr: 4.927 ± 0.811
5.368AlaVal: 5.368 ± 0.576
1.25AlaTrp: 1.25 ± 0.297
2.794AlaTyr: 2.794 ± 0.45
0.0AlaXaa: 0.0 ± 0.0
Cys
0.735CysAla: 0.735 ± 0.194
0.441CysCys: 0.441 ± 0.185
0.956CysAsp: 0.956 ± 0.243
1.029CysGlu: 1.029 ± 0.287
0.368CysPhe: 0.368 ± 0.163
1.691CysGly: 1.691 ± 0.434
0.662CysHis: 0.662 ± 0.223
0.515CysIle: 0.515 ± 0.164
1.324CysLys: 1.324 ± 0.375
0.882CysLeu: 0.882 ± 0.246
0.221CysMet: 0.221 ± 0.153
0.662CysAsn: 0.662 ± 0.228
0.588CysPro: 0.588 ± 0.21
0.368CysGln: 0.368 ± 0.162
0.662CysArg: 0.662 ± 0.183
0.588CysSer: 0.588 ± 0.22
0.662CysThr: 0.662 ± 0.239
0.662CysVal: 0.662 ± 0.229
0.368CysTrp: 0.368 ± 0.155
0.515CysTyr: 0.515 ± 0.187
0.0CysXaa: 0.0 ± 0.0
Asp
6.692AspAla: 6.692 ± 0.74
0.809AspCys: 0.809 ± 0.277
4.78AspAsp: 4.78 ± 0.77
4.78AspGlu: 4.78 ± 0.583
2.941AspPhe: 2.941 ± 0.452
5.662AspGly: 5.662 ± 0.814
0.882AspHis: 0.882 ± 0.272
4.633AspIle: 4.633 ± 0.532
3.603AspLys: 3.603 ± 0.582
3.088AspLeu: 3.088 ± 0.469
2.28AspMet: 2.28 ± 0.408
2.353AspAsn: 2.353 ± 0.37
1.544AspPro: 1.544 ± 0.362
1.103AspGln: 1.103 ± 0.322
2.353AspArg: 2.353 ± 0.419
3.088AspSer: 3.088 ± 0.472
2.721AspThr: 2.721 ± 0.486
4.559AspVal: 4.559 ± 0.591
0.735AspTrp: 0.735 ± 0.204
2.574AspTyr: 2.574 ± 0.381
0.0AspXaa: 0.0 ± 0.0
Glu
5.809GluAla: 5.809 ± 0.64
1.324GluCys: 1.324 ± 0.328
3.824GluAsp: 3.824 ± 0.716
3.971GluGlu: 3.971 ± 0.532
1.985GluPhe: 1.985 ± 0.372
2.868GluGly: 2.868 ± 0.535
0.809GluHis: 0.809 ± 0.216
4.412GluIle: 4.412 ± 0.526
4.044GluLys: 4.044 ± 0.579
6.398GluLeu: 6.398 ± 0.571
1.985GluMet: 1.985 ± 0.397
2.28GluAsn: 2.28 ± 0.407
1.912GluPro: 1.912 ± 0.298
3.897GluGln: 3.897 ± 0.476
3.309GluArg: 3.309 ± 0.567
3.53GluSer: 3.53 ± 0.474
3.162GluThr: 3.162 ± 0.444
4.191GluVal: 4.191 ± 0.497
1.544GluTrp: 1.544 ± 0.282
3.162GluTyr: 3.162 ± 0.463
0.0GluXaa: 0.0 ± 0.0
Phe
2.059PheAla: 2.059 ± 0.39
0.662PheCys: 0.662 ± 0.232
3.088PheAsp: 3.088 ± 0.424
2.427PheGlu: 2.427 ± 0.425
1.25PhePhe: 1.25 ± 0.303
3.088PheGly: 3.088 ± 0.423
0.588PheHis: 0.588 ± 0.203
3.162PheIle: 3.162 ± 0.513
1.471PheLys: 1.471 ± 0.321
2.059PheLeu: 2.059 ± 0.435
1.25PheMet: 1.25 ± 0.291
2.868PheAsn: 2.868 ± 0.545
1.177PhePro: 1.177 ± 0.287
1.177PheGln: 1.177 ± 0.302
2.059PheArg: 2.059 ± 0.429
2.721PheSer: 2.721 ± 0.427
1.544PheThr: 1.544 ± 0.385
1.765PheVal: 1.765 ± 0.359
0.368PheTrp: 0.368 ± 0.146
1.25PheTyr: 1.25 ± 0.318
0.0PheXaa: 0.0 ± 0.0
Gly
6.618GlyAla: 6.618 ± 0.687
1.029GlyCys: 1.029 ± 0.282
4.412GlyAsp: 4.412 ± 0.604
4.633GlyGlu: 4.633 ± 0.608
2.794GlyPhe: 2.794 ± 0.526
4.706GlyGly: 4.706 ± 0.631
1.103GlyHis: 1.103 ± 0.293
4.191GlyIle: 4.191 ± 0.474
5.589GlyLys: 5.589 ± 0.782
4.044GlyLeu: 4.044 ± 0.483
2.574GlyMet: 2.574 ± 0.406
2.941GlyAsn: 2.941 ± 0.396
0.956GlyPro: 0.956 ± 0.305
2.5GlyGln: 2.5 ± 0.471
3.015GlyArg: 3.015 ± 0.396
4.339GlySer: 4.339 ± 0.523
4.118GlyThr: 4.118 ± 0.695
4.633GlyVal: 4.633 ± 0.714
1.25GlyTrp: 1.25 ± 0.322
3.897GlyTyr: 3.897 ± 0.501
0.0GlyXaa: 0.0 ± 0.0
His
0.956HisAla: 0.956 ± 0.321
0.441HisCys: 0.441 ± 0.19
1.177HisAsp: 1.177 ± 0.294
0.956HisGlu: 0.956 ± 0.246
0.515HisPhe: 0.515 ± 0.164
1.324HisGly: 1.324 ± 0.285
0.147HisHis: 0.147 ± 0.109
0.515HisIle: 0.515 ± 0.199
1.177HisLys: 1.177 ± 0.308
1.397HisLeu: 1.397 ± 0.275
0.588HisMet: 0.588 ± 0.172
1.029HisAsn: 1.029 ± 0.301
0.809HisPro: 0.809 ± 0.246
0.735HisGln: 0.735 ± 0.188
0.882HisArg: 0.882 ± 0.271
0.441HisSer: 0.441 ± 0.175
0.809HisThr: 0.809 ± 0.26
1.25HisVal: 1.25 ± 0.333
0.221HisTrp: 0.221 ± 0.118
0.882HisTyr: 0.882 ± 0.262
0.0HisXaa: 0.0 ± 0.0
Ile
5.442IleAla: 5.442 ± 0.665
1.103IleCys: 1.103 ± 0.317
5.147IleAsp: 5.147 ± 0.567
4.412IleGlu: 4.412 ± 0.458
2.427IlePhe: 2.427 ± 0.413
4.927IleGly: 4.927 ± 0.663
1.25IleHis: 1.25 ± 0.254
4.559IleIle: 4.559 ± 0.794
4.191IleLys: 4.191 ± 0.424
4.265IleLeu: 4.265 ± 0.528
2.059IleMet: 2.059 ± 0.486
3.088IleAsn: 3.088 ± 0.457
2.427IlePro: 2.427 ± 0.339
1.691IleGln: 1.691 ± 0.297
3.824IleArg: 3.824 ± 0.508
3.971IleSer: 3.971 ± 0.582
4.191IleThr: 4.191 ± 0.514
4.265IleVal: 4.265 ± 0.389
0.662IleTrp: 0.662 ± 0.227
1.765IleTyr: 1.765 ± 0.365
0.0IleXaa: 0.0 ± 0.0
Lys
6.03LysAla: 6.03 ± 0.631
1.324LysCys: 1.324 ± 0.373
3.53LysAsp: 3.53 ± 0.512
3.456LysGlu: 3.456 ± 0.514
1.985LysPhe: 1.985 ± 0.379
3.53LysGly: 3.53 ± 0.449
1.029LysHis: 1.029 ± 0.295
4.191LysIle: 4.191 ± 0.566
3.971LysLys: 3.971 ± 0.587
5.442LysLeu: 5.442 ± 0.755
2.868LysMet: 2.868 ± 0.496
3.456LysAsn: 3.456 ± 0.609
2.574LysPro: 2.574 ± 0.426
2.941LysGln: 2.941 ± 0.486
3.603LysArg: 3.603 ± 0.574
4.044LysSer: 4.044 ± 0.57
3.236LysThr: 3.236 ± 0.455
4.486LysVal: 4.486 ± 0.561
1.25LysTrp: 1.25 ± 0.276
2.353LysTyr: 2.353 ± 0.442
0.0LysXaa: 0.0 ± 0.0
Leu
6.324LeuAla: 6.324 ± 0.864
1.029LeuCys: 1.029 ± 0.292
3.456LeuAsp: 3.456 ± 0.493
4.412LeuGlu: 4.412 ± 0.575
2.353LeuPhe: 2.353 ± 0.378
4.044LeuGly: 4.044 ± 0.501
0.956LeuHis: 0.956 ± 0.3
4.044LeuIle: 4.044 ± 0.521
5.221LeuLys: 5.221 ± 0.733
4.927LeuLeu: 4.927 ± 0.614
1.912LeuMet: 1.912 ± 0.354
3.75LeuAsn: 3.75 ± 0.535
3.236LeuPro: 3.236 ± 0.538
3.309LeuGln: 3.309 ± 0.477
4.412LeuArg: 4.412 ± 0.6
5.295LeuSer: 5.295 ± 0.671
5.589LeuThr: 5.589 ± 0.575
4.486LeuVal: 4.486 ± 0.746
1.618LeuTrp: 1.618 ± 0.342
2.574LeuTyr: 2.574 ± 0.402
0.0LeuXaa: 0.0 ± 0.0
Met
3.015MetAla: 3.015 ± 0.503
0.294MetCys: 0.294 ± 0.156
1.838MetAsp: 1.838 ± 0.366
1.471MetGlu: 1.471 ± 0.287
1.324MetPhe: 1.324 ± 0.326
1.103MetGly: 1.103 ± 0.291
0.662MetHis: 0.662 ± 0.271
2.353MetIle: 2.353 ± 0.443
3.53MetLys: 3.53 ± 0.561
1.838MetLeu: 1.838 ± 0.288
1.177MetMet: 1.177 ± 0.305
1.912MetAsn: 1.912 ± 0.313
1.618MetPro: 1.618 ± 0.335
1.618MetGln: 1.618 ± 0.381
1.765MetArg: 1.765 ± 0.299
2.28MetSer: 2.28 ± 0.469
2.059MetThr: 2.059 ± 0.466
1.324MetVal: 1.324 ± 0.325
0.662MetTrp: 0.662 ± 0.214
0.882MetTyr: 0.882 ± 0.211
0.0MetXaa: 0.0 ± 0.0
Asn
5.0AsnAla: 5.0 ± 0.669
0.294AsnCys: 0.294 ± 0.15
2.941AsnAsp: 2.941 ± 0.388
2.647AsnGlu: 2.647 ± 0.357
1.177AsnPhe: 1.177 ± 0.311
5.147AsnGly: 5.147 ± 0.711
1.029AsnHis: 1.029 ± 0.291
2.5AsnIle: 2.5 ± 0.442
3.015AsnLys: 3.015 ± 0.43
3.897AsnLeu: 3.897 ± 0.553
1.029AsnMet: 1.029 ± 0.234
2.353AsnAsn: 2.353 ± 0.509
1.618AsnPro: 1.618 ± 0.316
1.838AsnGln: 1.838 ± 0.381
2.427AsnArg: 2.427 ± 0.406
2.721AsnSer: 2.721 ± 0.393
2.133AsnThr: 2.133 ± 0.392
3.53AsnVal: 3.53 ± 0.597
0.809AsnTrp: 0.809 ± 0.237
1.471AsnTyr: 1.471 ± 0.298
0.0AsnXaa: 0.0 ± 0.0
Pro
3.677ProAla: 3.677 ± 0.499
0.368ProCys: 0.368 ± 0.165
2.5ProAsp: 2.5 ± 0.394
2.427ProGlu: 2.427 ± 0.407
1.324ProPhe: 1.324 ± 0.322
2.206ProGly: 2.206 ± 0.413
0.588ProHis: 0.588 ± 0.183
2.28ProIle: 2.28 ± 0.374
1.691ProLys: 1.691 ± 0.337
2.133ProLeu: 2.133 ± 0.469
1.029ProMet: 1.029 ± 0.215
1.029ProAsn: 1.029 ± 0.234
0.882ProPro: 0.882 ± 0.244
1.25ProGln: 1.25 ± 0.311
0.882ProArg: 0.882 ± 0.236
2.059ProSer: 2.059 ± 0.38
1.618ProThr: 1.618 ± 0.464
3.236ProVal: 3.236 ± 0.512
0.515ProTrp: 0.515 ± 0.293
1.471ProTyr: 1.471 ± 0.385
0.0ProXaa: 0.0 ± 0.0
Gln
3.75GlnAla: 3.75 ± 0.746
0.441GlnCys: 0.441 ± 0.188
1.397GlnAsp: 1.397 ± 0.41
2.794GlnGlu: 2.794 ± 0.477
1.765GlnPhe: 1.765 ± 0.398
1.544GlnGly: 1.544 ± 0.343
1.25GlnHis: 1.25 ± 0.321
2.427GlnIle: 2.427 ± 0.496
2.721GlnLys: 2.721 ± 0.486
3.603GlnLeu: 3.603 ± 0.48
1.618GlnMet: 1.618 ± 0.4
1.177GlnAsn: 1.177 ± 0.236
1.618GlnPro: 1.618 ± 0.314
2.941GlnGln: 2.941 ± 1.046
1.765GlnArg: 1.765 ± 0.364
3.236GlnSer: 3.236 ± 0.519
2.206GlnThr: 2.206 ± 0.556
1.691GlnVal: 1.691 ± 0.427
0.588GlnTrp: 0.588 ± 0.215
1.471GlnTyr: 1.471 ± 0.267
0.0GlnXaa: 0.0 ± 0.0
Arg
4.118ArgAla: 4.118 ± 0.35
1.25ArgCys: 1.25 ± 0.4
2.721ArgAsp: 2.721 ± 0.493
3.824ArgGlu: 3.824 ± 0.593
1.765ArgPhe: 1.765 ± 0.331
3.162ArgGly: 3.162 ± 0.518
0.882ArgHis: 0.882 ± 0.219
3.383ArgIle: 3.383 ± 0.485
3.309ArgLys: 3.309 ± 0.55
4.486ArgLeu: 4.486 ± 0.672
1.25ArgMet: 1.25 ± 0.343
3.75ArgAsn: 3.75 ± 0.496
1.103ArgPro: 1.103 ± 0.311
1.838ArgGln: 1.838 ± 0.427
2.794ArgArg: 2.794 ± 0.45
2.574ArgSer: 2.574 ± 0.423
1.618ArgThr: 1.618 ± 0.414
3.236ArgVal: 3.236 ± 0.541
0.809ArgTrp: 0.809 ± 0.285
3.015ArgTyr: 3.015 ± 0.402
0.0ArgXaa: 0.0 ± 0.0
Ser
6.986SerAla: 6.986 ± 1.05
0.662SerCys: 0.662 ± 0.252
3.971SerAsp: 3.971 ± 0.444
3.603SerGlu: 3.603 ± 0.576
2.794SerPhe: 2.794 ± 0.441
5.147SerGly: 5.147 ± 0.545
0.662SerHis: 0.662 ± 0.247
3.75SerIle: 3.75 ± 0.472
3.603SerLys: 3.603 ± 0.466
5.0SerLeu: 5.0 ± 0.54
2.133SerMet: 2.133 ± 0.448
2.427SerAsn: 2.427 ± 0.442
2.353SerPro: 2.353 ± 0.353
2.721SerGln: 2.721 ± 0.514
2.574SerArg: 2.574 ± 0.368
4.118SerSer: 4.118 ± 0.737
3.897SerThr: 3.897 ± 0.674
4.265SerVal: 4.265 ± 0.512
0.735SerTrp: 0.735 ± 0.26
2.133SerTyr: 2.133 ± 0.367
0.0SerXaa: 0.0 ± 0.0
Thr
4.78ThrAla: 4.78 ± 0.53
0.441ThrCys: 0.441 ± 0.149
3.75ThrAsp: 3.75 ± 0.508
3.971ThrGlu: 3.971 ± 0.513
1.765ThrPhe: 1.765 ± 0.391
5.368ThrGly: 5.368 ± 0.612
1.029ThrHis: 1.029 ± 0.289
3.53ThrIle: 3.53 ± 0.433
3.383ThrLys: 3.383 ± 0.548
3.162ThrLeu: 3.162 ± 0.579
1.397ThrMet: 1.397 ± 0.327
2.868ThrAsn: 2.868 ± 0.49
3.162ThrPro: 3.162 ± 0.541
1.618ThrGln: 1.618 ± 0.305
2.647ThrArg: 2.647 ± 0.462
3.971ThrSer: 3.971 ± 0.657
3.75ThrThr: 3.75 ± 0.722
3.162ThrVal: 3.162 ± 0.526
0.809ThrTrp: 0.809 ± 0.304
2.206ThrTyr: 2.206 ± 0.387
0.0ThrXaa: 0.0 ± 0.0
Val
5.368ValAla: 5.368 ± 0.722
0.074ValCys: 0.074 ± 0.076
3.088ValAsp: 3.088 ± 0.544
3.971ValGlu: 3.971 ± 0.526
2.28ValPhe: 2.28 ± 0.438
3.603ValGly: 3.603 ± 0.582
0.735ValHis: 0.735 ± 0.218
6.03ValIle: 6.03 ± 0.711
3.897ValLys: 3.897 ± 0.535
3.971ValLeu: 3.971 ± 0.668
2.28ValMet: 2.28 ± 0.399
3.309ValAsn: 3.309 ± 0.523
1.397ValPro: 1.397 ± 0.382
2.5ValGln: 2.5 ± 0.407
3.603ValArg: 3.603 ± 0.527
5.589ValSer: 5.589 ± 0.668
4.706ValThr: 4.706 ± 0.724
4.191ValVal: 4.191 ± 0.723
1.177ValTrp: 1.177 ± 0.28
2.427ValTyr: 2.427 ± 0.344
0.0ValXaa: 0.0 ± 0.0
Trp
1.103TrpAla: 1.103 ± 0.274
0.221TrpCys: 0.221 ± 0.124
1.25TrpAsp: 1.25 ± 0.256
0.882TrpGlu: 0.882 ± 0.263
0.515TrpPhe: 0.515 ± 0.215
0.368TrpGly: 0.368 ± 0.194
0.588TrpHis: 0.588 ± 0.245
1.029TrpIle: 1.029 ± 0.331
1.25TrpLys: 1.25 ± 0.284
1.691TrpLeu: 1.691 ± 0.387
0.221TrpMet: 0.221 ± 0.125
0.588TrpAsn: 0.588 ± 0.202
0.441TrpPro: 0.441 ± 0.193
0.956TrpGln: 0.956 ± 0.297
1.177TrpArg: 1.177 ± 0.303
0.735TrpSer: 0.735 ± 0.199
1.103TrpThr: 1.103 ± 0.267
1.544TrpVal: 1.544 ± 0.327
0.368TrpTrp: 0.368 ± 0.177
0.515TrpTyr: 0.515 ± 0.163
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.456TyrAla: 3.456 ± 0.486
0.662TyrCys: 0.662 ± 0.198
2.941TyrAsp: 2.941 ± 0.48
2.427TyrGlu: 2.427 ± 0.383
1.691TyrPhe: 1.691 ± 0.348
2.868TyrGly: 2.868 ± 0.446
0.588TyrHis: 0.588 ± 0.212
2.133TyrIle: 2.133 ± 0.448
2.133TyrLys: 2.133 ± 0.436
2.721TyrLeu: 2.721 ± 0.572
0.588TyrMet: 0.588 ± 0.207
1.618TyrAsn: 1.618 ± 0.315
1.544TyrPro: 1.544 ± 0.351
1.397TyrGln: 1.397 ± 0.3
1.618TyrArg: 1.618 ± 0.275
3.088TyrSer: 3.088 ± 0.442
2.721TyrThr: 2.721 ± 0.541
2.353TyrVal: 2.353 ± 0.402
0.809TyrTrp: 0.809 ± 0.217
1.25TyrTyr: 1.25 ± 0.357
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 76 proteins (13600 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski