Streptomyces phage Hiyaa

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Hiyaavirus; Streptomyces virus Hiyaa

Average proteome isoelectric point is 6.27

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 124 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3S9U8K9|A0A3S9U8K9_9CAUD Head-to-tail adaptor OS=Streptomyces phage Hiyaa OX=2499072 GN=14 PE=4 SV=1
MM1 pKa = 7.66ANLVTNIGKK10 pKa = 9.71GRR12 pKa = 11.84FVHH15 pKa = 6.19YY16 pKa = 8.28ATLPATNDD24 pKa = 3.08ALIAVVLEE32 pKa = 4.18ATGLEE37 pKa = 4.3ADD39 pKa = 5.48DD40 pKa = 5.4ALQDD44 pKa = 3.96YY45 pKa = 10.98DD46 pKa = 5.61DD47 pKa = 5.0LAALLAGASNEE58 pKa = 3.86QTTMGRR64 pKa = 11.84KK65 pKa = 6.82TLANVTVNVNDD76 pKa = 3.96TANTASIDD84 pKa = 3.86CDD86 pKa = 3.63DD87 pKa = 4.35VTWTAATGNATGKK100 pKa = 10.55LVVCYY105 pKa = 10.63DD106 pKa = 3.87PDD108 pKa = 3.76TTAGTDD114 pKa = 3.34SSIIPLTLHH123 pKa = 6.76DD124 pKa = 5.11FSVTPDD130 pKa = 3.22GTDD133 pKa = 2.5ITVQINAAGLATAANAA149 pKa = 3.93

Molecular weight: 15.26 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3S9U8N5|A0A3S9U8N5_9CAUD Uncharacterized protein OS=Streptomyces phage Hiyaa OX=2499072 GN=36 PE=4 SV=1
MM1 pKa = 7.38NKK3 pKa = 9.08EE4 pKa = 3.36QAAWGRR10 pKa = 11.84LLEE13 pKa = 4.27SAPRR17 pKa = 11.84GRR19 pKa = 11.84YY20 pKa = 6.23EE21 pKa = 5.29HH22 pKa = 6.57ARR24 pKa = 11.84SWQKK28 pKa = 10.44RR29 pKa = 11.84EE30 pKa = 3.48KK31 pKa = 10.4RR32 pKa = 11.84RR33 pKa = 11.84ANFRR37 pKa = 11.84AKK39 pKa = 10.31VSRR42 pKa = 11.84QPGGCWLWVGQSPVRR57 pKa = 11.84SGKK60 pKa = 9.51PYY62 pKa = 9.74PLWGVKK68 pKa = 9.57IKK70 pKa = 10.59GRR72 pKa = 11.84SQQRR76 pKa = 11.84SAFVWMIGEE85 pKa = 4.48FFPEE89 pKa = 4.17LAATLPHH96 pKa = 6.46RR97 pKa = 11.84TQPSCGEE104 pKa = 4.27STCIAPTHH112 pKa = 6.23RR113 pKa = 11.84VNGMLTKK120 pKa = 10.7NVITPEE126 pKa = 3.69QAIEE130 pKa = 3.94LFSLKK135 pKa = 10.09GHH137 pKa = 7.13ADD139 pKa = 3.39SQEE142 pKa = 3.76VAEE145 pKa = 4.55RR146 pKa = 11.84FGISRR151 pKa = 11.84NQVLSIWRR159 pKa = 11.84GRR161 pKa = 11.84NWGNVTGAVNQTRR174 pKa = 11.84QRR176 pKa = 11.84KK177 pKa = 6.34VTPPDD182 pKa = 2.99IVDD185 pKa = 4.84AIKK188 pKa = 10.61DD189 pKa = 3.55RR190 pKa = 11.84HH191 pKa = 5.24GTASSRR197 pKa = 11.84AVAAEE202 pKa = 3.86FNVSYY207 pKa = 8.93KK208 pKa = 9.82TVLRR212 pKa = 11.84IWNDD216 pKa = 2.95EE217 pKa = 3.69HH218 pKa = 7.48RR219 pKa = 11.84GLSRR223 pKa = 11.84EE224 pKa = 3.97QAPCC228 pKa = 3.68

Molecular weight: 25.85 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

124

0

124

24492

29

1110

197.5

21.66

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.755 ± 0.349

0.972 ± 0.117

6.667 ± 0.18

6.239 ± 0.329

2.699 ± 0.108

8.562 ± 0.194

1.96 ± 0.125

4.144 ± 0.193

4.205 ± 0.267

7.574 ± 0.208

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.274 ± 0.122

3.283 ± 0.138

5.234 ± 0.277

3.752 ± 0.129

7.174 ± 0.289

5.32 ± 0.273

6.843 ± 0.317

7.782 ± 0.221

2.135 ± 0.131

2.425 ± 0.124
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski