Reovirus type 3 (strain Dearing) (T3D) (Mammalian orthoreovirus 3)

Taxonomy: Viruses; Riboviria; Orthornavirae; Duplornaviricota; Resentoviricetes; Reovirales; Reoviridae; Spinareovirinae; Orthoreovirus; Mammalian orthoreovirus; Mammalian orthoreovirus 3

Average proteome isoelectric point is 5.79

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 11 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>sp|P12418|MU2_REOVD Microtubule-associated protein mu-2 OS=Reovirus type 3 (strain Dearing) OX=10886 GN=M1 PE=1 SV=1
MM1 pKa = 7.34ANVWGVRR8 pKa = 11.84LADD11 pKa = 3.82SLSSPTIEE19 pKa = 4.01TRR21 pKa = 11.84TRR23 pKa = 11.84QYY25 pKa = 10.47TLHH28 pKa = 7.18DD29 pKa = 4.26LCSDD33 pKa = 3.92LDD35 pKa = 4.12ANPGRR40 pKa = 11.84EE41 pKa = 3.64PWKK44 pKa = 9.54PLRR47 pKa = 11.84NQRR50 pKa = 11.84TNNIVAVQLFRR61 pKa = 11.84PLQGLVLDD69 pKa = 4.01TQLYY73 pKa = 8.21GFPGAFDD80 pKa = 3.07DD81 pKa = 3.81WEE83 pKa = 4.65RR84 pKa = 11.84FMRR87 pKa = 11.84EE88 pKa = 3.38KK89 pKa = 11.01LRR91 pKa = 11.84VLKK94 pKa = 11.15YY95 pKa = 9.5EE96 pKa = 3.91VLRR99 pKa = 11.84IYY101 pKa = 10.23PISNYY106 pKa = 10.13SNEE109 pKa = 4.08HH110 pKa = 5.13VNVFVANALVGAFLSNQAFYY130 pKa = 11.48DD131 pKa = 4.14LLPLLIINDD140 pKa = 3.8TMIGDD145 pKa = 4.58LLGTGASLSQFFQSHH160 pKa = 6.04GDD162 pKa = 3.68VLEE165 pKa = 4.24VAAGRR170 pKa = 11.84KK171 pKa = 7.8YY172 pKa = 10.94LQMEE176 pKa = 4.56NYY178 pKa = 10.61SNDD181 pKa = 3.83DD182 pKa = 3.58DD183 pKa = 5.86DD184 pKa = 5.56PPLFAKK190 pKa = 10.55DD191 pKa = 3.38LSDD194 pKa = 3.66YY195 pKa = 11.6AKK197 pKa = 10.8AFYY200 pKa = 10.69SDD202 pKa = 3.4TYY204 pKa = 10.92EE205 pKa = 3.91VLDD208 pKa = 3.77RR209 pKa = 11.84FFWTHH214 pKa = 6.75DD215 pKa = 3.09SSAGVLVHH223 pKa = 6.38YY224 pKa = 8.23DD225 pKa = 3.06KK226 pKa = 10.18PTNGHH231 pKa = 6.61HH232 pKa = 6.2YY233 pKa = 11.06LLGTLTQMVSAPPYY247 pKa = 10.06IINATDD253 pKa = 3.2AMLLEE258 pKa = 4.74SCLEE262 pKa = 3.89QFSANVRR269 pKa = 11.84ARR271 pKa = 11.84PAQPVTRR278 pKa = 11.84LDD280 pKa = 3.2QCYY283 pKa = 9.51HH284 pKa = 5.99LRR286 pKa = 11.84WGAQYY291 pKa = 10.87VGEE294 pKa = 4.72DD295 pKa = 3.3SLTYY299 pKa = 10.4RR300 pKa = 11.84LGVLSLLATNGYY312 pKa = 9.17QLARR316 pKa = 11.84PIPRR320 pKa = 11.84QLTNRR325 pKa = 11.84WLSSFVSQIMSDD337 pKa = 3.81GVNEE341 pKa = 4.18TPLWPQEE348 pKa = 4.03RR349 pKa = 11.84YY350 pKa = 8.46VQIAYY355 pKa = 9.43DD356 pKa = 3.79SPSVVDD362 pKa = 4.59GATQYY367 pKa = 11.67GYY369 pKa = 10.63VRR371 pKa = 11.84KK372 pKa = 9.02NQLRR376 pKa = 11.84LGMRR380 pKa = 11.84ISALQSLSDD389 pKa = 3.84TPSPVQWLPQYY400 pKa = 10.55TIDD403 pKa = 4.28QAAMDD408 pKa = 4.7EE409 pKa = 4.25GDD411 pKa = 4.05LMVSRR416 pKa = 11.84LTQLPLRR423 pKa = 11.84PDD425 pKa = 3.52YY426 pKa = 11.63GNIWVGDD433 pKa = 3.82ALSYY437 pKa = 10.3YY438 pKa = 10.06VDD440 pKa = 3.67YY441 pKa = 11.11NRR443 pKa = 11.84SHH445 pKa = 6.66RR446 pKa = 11.84VVLSSEE452 pKa = 4.47LPQLPDD458 pKa = 2.96TYY460 pKa = 11.3FDD462 pKa = 3.83GDD464 pKa = 3.65EE465 pKa = 4.0QYY467 pKa = 11.5GRR469 pKa = 11.84SLFSLARR476 pKa = 11.84KK477 pKa = 9.61IGDD480 pKa = 3.21RR481 pKa = 11.84SLVKK485 pKa = 9.91DD486 pKa = 3.47TAVLKK491 pKa = 10.42HH492 pKa = 6.56AYY494 pKa = 8.89QAIDD498 pKa = 3.52PNTGKK503 pKa = 10.6EE504 pKa = 4.1YY505 pKa = 10.63LRR507 pKa = 11.84SRR509 pKa = 11.84QSVAYY514 pKa = 8.95FGASAGHH521 pKa = 6.32SGADD525 pKa = 3.12QPLVIEE531 pKa = 4.54PWIQGKK537 pKa = 9.89ISGVPPPSSVRR548 pKa = 11.84QFGYY552 pKa = 10.76DD553 pKa = 3.29VARR556 pKa = 11.84GAIVDD561 pKa = 4.35LARR564 pKa = 11.84PFPSGDD570 pKa = 3.41YY571 pKa = 10.22QFVYY575 pKa = 10.9SDD577 pKa = 3.15VDD579 pKa = 3.69QVVDD583 pKa = 3.69GHH585 pKa = 7.25DD586 pKa = 4.11DD587 pKa = 3.62LSISSGLVEE596 pKa = 4.94SLLSSCMHH604 pKa = 6.35ATAPGGSFVVKK615 pKa = 10.23INFPTRR621 pKa = 11.84PVWHH625 pKa = 6.69YY626 pKa = 10.51IEE628 pKa = 5.3QKK630 pKa = 9.72ILPNITSYY638 pKa = 10.97MLIKK642 pKa = 10.53PFVTNNVEE650 pKa = 4.06LFFVAFGVHH659 pKa = 4.71QHH661 pKa = 6.6SSLTWTSGVYY671 pKa = 10.02FFLVDD676 pKa = 3.09HH677 pKa = 7.04FYY679 pKa = 10.67RR680 pKa = 11.84YY681 pKa = 7.46EE682 pKa = 3.98TLSTISRR689 pKa = 11.84QLPSFGYY696 pKa = 10.34VDD698 pKa = 4.85DD699 pKa = 5.1GSSVTGIEE707 pKa = 4.39TISIEE712 pKa = 4.16NPGFSNMTQAARR724 pKa = 11.84IGISGLCANVGNARR738 pKa = 11.84KK739 pKa = 9.98SIAIYY744 pKa = 8.9EE745 pKa = 4.3SHH747 pKa = 6.82GARR750 pKa = 11.84VLTITSRR757 pKa = 11.84RR758 pKa = 11.84SPASARR764 pKa = 11.84RR765 pKa = 11.84KK766 pKa = 9.65SRR768 pKa = 11.84LRR770 pKa = 11.84YY771 pKa = 9.57LPLIDD776 pKa = 4.18PRR778 pKa = 11.84SLEE781 pKa = 4.04VQARR785 pKa = 11.84TILPADD791 pKa = 3.38PVLFEE796 pKa = 4.41NVSGASPHH804 pKa = 5.75VCLTMMYY811 pKa = 9.72NFEE814 pKa = 4.24VSSAVYY820 pKa = 10.61DD821 pKa = 3.73GDD823 pKa = 3.94VVLDD827 pKa = 4.5LGTGPEE833 pKa = 3.96AKK835 pKa = 9.76ILEE838 pKa = 5.29LIPATSPVTCVDD850 pKa = 3.28IRR852 pKa = 11.84PTAQPSGCWNVRR864 pKa = 11.84TTFLEE869 pKa = 3.91LDD871 pKa = 3.83YY872 pKa = 11.51LSDD875 pKa = 3.26GWITGVRR882 pKa = 11.84GDD884 pKa = 4.46IVTCMLSLGAAAAGKK899 pKa = 10.91SMTFDD904 pKa = 3.32AAFQQLIKK912 pKa = 10.78VLSKK916 pKa = 9.66STANVVLVQVNCPTDD931 pKa = 3.4VVRR934 pKa = 11.84SIKK937 pKa = 10.54GYY939 pKa = 10.91LEE941 pKa = 3.89IDD943 pKa = 3.83STNKK947 pKa = 10.08RR948 pKa = 11.84YY949 pKa = 10.15RR950 pKa = 11.84FPKK953 pKa = 10.01FGRR956 pKa = 11.84DD957 pKa = 3.29EE958 pKa = 4.96PYY960 pKa = 10.91SDD962 pKa = 3.58MDD964 pKa = 4.02ALEE967 pKa = 4.72KK968 pKa = 10.16ICRR971 pKa = 11.84TAWPNCSITWVPLSYY986 pKa = 10.89DD987 pKa = 3.71LRR989 pKa = 11.84WTRR992 pKa = 11.84LALLEE997 pKa = 4.23STTLSSASIRR1007 pKa = 11.84IAEE1010 pKa = 4.22LMYY1013 pKa = 10.6KK1014 pKa = 9.97YY1015 pKa = 9.76MPIMRR1020 pKa = 11.84IDD1022 pKa = 3.18IHH1024 pKa = 6.13GLPMEE1029 pKa = 4.29KK1030 pKa = 9.6RR1031 pKa = 11.84GNFIVGQNCSLVIPGFNAQDD1051 pKa = 3.46VFNCYY1056 pKa = 9.73FNSALAFSTEE1066 pKa = 4.13DD1067 pKa = 3.22VNAAMIPQVSAQFDD1081 pKa = 3.63ATKK1084 pKa = 11.06GEE1086 pKa = 4.08WTLDD1090 pKa = 3.42MVFSDD1095 pKa = 3.23AGIYY1099 pKa = 8.99TMQALVGSNANPVSLGSFVVDD1120 pKa = 4.37SPDD1123 pKa = 4.22VDD1125 pKa = 3.82ITDD1128 pKa = 3.6AWPAQLDD1135 pKa = 3.67FTIAGTDD1142 pKa = 2.91VDD1144 pKa = 3.83ITVNPYY1150 pKa = 8.82YY1151 pKa = 10.96RR1152 pKa = 11.84LMTFVRR1158 pKa = 11.84IDD1160 pKa = 3.81GQWQIANPDD1169 pKa = 3.1KK1170 pKa = 11.12FQFFSSASGTLVMNVKK1186 pKa = 10.45LDD1188 pKa = 3.41IADD1191 pKa = 4.19KK1192 pKa = 10.68YY1193 pKa = 10.02LLYY1196 pKa = 10.57YY1197 pKa = 9.88IRR1199 pKa = 11.84DD1200 pKa = 3.64VQSRR1204 pKa = 11.84DD1205 pKa = 3.04VGFYY1209 pKa = 9.9IQHH1212 pKa = 7.14PLQLLNTITLPTNEE1226 pKa = 5.26DD1227 pKa = 3.81LFLSAPDD1234 pKa = 3.44MRR1236 pKa = 11.84EE1237 pKa = 3.61WAVKK1241 pKa = 10.18EE1242 pKa = 4.15SGNTICILNSQGFVLPQDD1260 pKa = 3.21WDD1262 pKa = 3.8VLTDD1266 pKa = 4.23TISWSPSIPTYY1277 pKa = 10.24IVPPGDD1283 pKa = 3.52YY1284 pKa = 10.21TLTPLL1289 pKa = 4.79

Molecular weight:
143.98 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>sp|P03526|SIGNS_REOVD Protein sigma-NS OS=Reovirus type 3 (strain Dearing) OX=10886 GN=S3 PE=2 SV=1
MM1 pKa = 7.31ARR3 pKa = 11.84AAFLFKK9 pKa = 10.33TVGFGGLQNVPINDD23 pKa = 3.83EE24 pKa = 4.26LSSHH28 pKa = 6.8LLRR31 pKa = 11.84AGNSPWQLTQFLDD44 pKa = 4.17WISLGRR50 pKa = 11.84GLATSALVPTAGSRR64 pKa = 11.84YY65 pKa = 8.74YY66 pKa = 11.13QMSCLLSGTLQIPFRR81 pKa = 11.84PNHH84 pKa = 5.6RR85 pKa = 11.84WGDD88 pKa = 3.35IRR90 pKa = 11.84FLRR93 pKa = 11.84LVWSAPTLDD102 pKa = 4.12GLVVAPPQVLAQPALQAQADD122 pKa = 4.12RR123 pKa = 11.84VYY125 pKa = 11.29DD126 pKa = 3.87CDD128 pKa = 5.2DD129 pKa = 3.71YY130 pKa = 11.78PFLARR135 pKa = 11.84DD136 pKa = 3.34PRR138 pKa = 11.84FKK140 pKa = 10.89HH141 pKa = 6.07RR142 pKa = 11.84VYY144 pKa = 10.5QQLSAVTLLNLTGFGPISYY163 pKa = 10.03VRR165 pKa = 11.84VDD167 pKa = 3.06EE168 pKa = 5.2DD169 pKa = 3.21MWSGDD174 pKa = 3.63VNQLLMNYY182 pKa = 9.49FGHH185 pKa = 6.38TFAEE189 pKa = 4.2IAYY192 pKa = 6.69TLCQASANRR201 pKa = 11.84PWEE204 pKa = 3.74YY205 pKa = 10.93DD206 pKa = 2.89GTYY209 pKa = 11.24ARR211 pKa = 11.84MTQIVLSLFWLSYY224 pKa = 10.67VGVIHH229 pKa = 6.07QQNTYY234 pKa = 8.17RR235 pKa = 11.84TFYY238 pKa = 9.35FQCNRR243 pKa = 11.84RR244 pKa = 11.84GDD246 pKa = 3.7AAEE249 pKa = 4.12VWILSCSLNHH259 pKa = 6.59SAQIRR264 pKa = 11.84PGNRR268 pKa = 11.84SLFVMPTSPDD278 pKa = 2.57WNMDD282 pKa = 3.01VNLILSSTLTGCLCSGSQLPLIDD305 pKa = 4.33NNSVPAVSRR314 pKa = 11.84NIHH317 pKa = 5.35GWTGRR322 pKa = 11.84AGNQLHH328 pKa = 6.48GFQVRR333 pKa = 11.84RR334 pKa = 11.84MVTEE338 pKa = 4.08FCDD341 pKa = 3.06RR342 pKa = 11.84LRR344 pKa = 11.84RR345 pKa = 11.84DD346 pKa = 3.17GVMTQAQQNQVEE358 pKa = 4.24ALADD362 pKa = 3.24QTQQFKK368 pKa = 10.57RR369 pKa = 11.84DD370 pKa = 3.67KK371 pKa = 10.52LEE373 pKa = 3.53TWARR377 pKa = 11.84EE378 pKa = 3.64DD379 pKa = 3.39DD380 pKa = 4.48QYY382 pKa = 12.13NQAHH386 pKa = 6.94PNSTMFRR393 pKa = 11.84TKK395 pKa = 10.55PFTNAQWGRR404 pKa = 11.84GNTGATSAAIAALII418 pKa = 4.13

Molecular weight:
47.16 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

10

1

11

8281

365

1289

752.8

83.84

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.006 ± 0.375

1.352 ± 0.152

6.461 ± 0.296

4.371 ± 0.292

3.502 ± 0.177

5.482 ± 0.369

2.005 ± 0.222

5.664 ± 0.219

3.768 ± 0.36

9.576 ± 0.353

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.236 ± 0.282

3.997 ± 0.363

5.482 ± 0.41

4.685 ± 0.347

5.651 ± 0.242

8.369 ± 0.304

6.135 ± 0.407

7.366 ± 0.354

1.473 ± 0.176

3.417 ± 0.328

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski