Amino acid dipepetide frequency for Escherichia phage HK542

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
10.699AlaAla: 10.699 ± 1.643
0.927AlaCys: 0.927 ± 0.343
7.077AlaAsp: 7.077 ± 0.831
6.487AlaGlu: 6.487 ± 0.732
3.033AlaPhe: 3.033 ± 0.504
8.256AlaGly: 8.256 ± 0.952
1.516AlaHis: 1.516 ± 0.429
6.571AlaIle: 6.571 ± 0.724
4.634AlaLys: 4.634 ± 0.767
7.835AlaLeu: 7.835 ± 0.704
3.033AlaMet: 3.033 ± 0.565
4.549AlaAsn: 4.549 ± 0.731
2.359AlaPro: 2.359 ± 0.386
5.055AlaGln: 5.055 ± 0.97
4.212AlaArg: 4.212 ± 0.507
6.487AlaSer: 6.487 ± 1.388
5.139AlaThr: 5.139 ± 0.874
5.981AlaVal: 5.981 ± 0.784
1.769AlaTrp: 1.769 ± 0.381
2.612AlaTyr: 2.612 ± 0.493
0.0AlaXaa: 0.0 ± 0.0
Cys
0.927CysAla: 0.927 ± 0.298
0.168CysCys: 0.168 ± 0.121
0.59CysAsp: 0.59 ± 0.191
0.674CysGlu: 0.674 ± 0.224
0.084CysPhe: 0.084 ± 0.087
1.095CysGly: 1.095 ± 0.337
0.421CysHis: 0.421 ± 0.231
0.927CysIle: 0.927 ± 0.268
0.59CysLys: 0.59 ± 0.248
0.674CysLeu: 0.674 ± 0.238
0.168CysMet: 0.168 ± 0.126
0.674CysAsn: 0.674 ± 0.218
0.337CysPro: 0.337 ± 0.212
0.421CysGln: 0.421 ± 0.204
0.674CysArg: 0.674 ± 0.26
0.842CysSer: 0.842 ± 0.312
0.337CysThr: 0.337 ± 0.133
0.505CysVal: 0.505 ± 0.252
0.168CysTrp: 0.168 ± 0.15
0.253CysTyr: 0.253 ± 0.192
0.0CysXaa: 0.0 ± 0.0
Asp
6.15AspAla: 6.15 ± 0.558
0.674AspCys: 0.674 ± 0.273
3.538AspAsp: 3.538 ± 0.64
3.538AspGlu: 3.538 ± 0.508
1.853AspPhe: 1.853 ± 0.426
5.307AspGly: 5.307 ± 0.683
0.674AspHis: 0.674 ± 0.275
2.612AspIle: 2.612 ± 0.439
2.612AspLys: 2.612 ± 0.492
4.802AspLeu: 4.802 ± 0.717
1.516AspMet: 1.516 ± 0.392
2.864AspAsn: 2.864 ± 0.48
1.769AspPro: 1.769 ± 0.413
2.106AspGln: 2.106 ± 0.402
3.538AspArg: 3.538 ± 0.613
3.707AspSer: 3.707 ± 0.574
1.938AspThr: 1.938 ± 0.513
4.802AspVal: 4.802 ± 0.763
0.674AspTrp: 0.674 ± 0.173
1.938AspTyr: 1.938 ± 0.381
0.0AspXaa: 0.0 ± 0.0
Glu
5.897GluAla: 5.897 ± 0.865
0.505GluCys: 0.505 ± 0.203
2.359GluAsp: 2.359 ± 0.36
5.055GluGlu: 5.055 ± 0.88
2.443GluPhe: 2.443 ± 0.375
4.044GluGly: 4.044 ± 0.669
1.011GluHis: 1.011 ± 0.348
3.538GluIle: 3.538 ± 0.456
3.454GluLys: 3.454 ± 0.52
5.981GluLeu: 5.981 ± 0.668
1.685GluMet: 1.685 ± 0.415
2.949GluAsn: 2.949 ± 0.467
1.853GluPro: 1.853 ± 0.366
3.791GluGln: 3.791 ± 0.57
3.201GluArg: 3.201 ± 0.545
4.212GluSer: 4.212 ± 0.591
3.201GluThr: 3.201 ± 0.5
3.37GluVal: 3.37 ± 0.615
0.758GluTrp: 0.758 ± 0.26
1.601GluTyr: 1.601 ± 0.332
0.0GluXaa: 0.0 ± 0.0
Phe
3.454PheAla: 3.454 ± 0.502
0.421PheCys: 0.421 ± 0.205
2.696PheAsp: 2.696 ± 0.396
1.685PheGlu: 1.685 ± 0.4
0.758PhePhe: 0.758 ± 0.236
2.612PheGly: 2.612 ± 0.452
0.337PheHis: 0.337 ± 0.149
2.275PheIle: 2.275 ± 0.557
1.348PheLys: 1.348 ± 0.327
1.432PheLeu: 1.432 ± 0.424
0.59PheMet: 0.59 ± 0.174
1.432PheAsn: 1.432 ± 0.285
1.516PhePro: 1.516 ± 0.377
0.758PheGln: 0.758 ± 0.216
1.769PheArg: 1.769 ± 0.407
2.106PheSer: 2.106 ± 0.443
3.286PheThr: 3.286 ± 0.569
1.601PheVal: 1.601 ± 0.29
0.674PheTrp: 0.674 ± 0.205
1.011PheTyr: 1.011 ± 0.309
0.0PheXaa: 0.0 ± 0.0
Gly
6.824GlyAla: 6.824 ± 1.076
0.674GlyCys: 0.674 ± 0.188
3.875GlyAsp: 3.875 ± 0.694
4.465GlyGlu: 4.465 ± 0.688
3.033GlyPhe: 3.033 ± 0.549
6.74GlyGly: 6.74 ± 1.148
0.842GlyHis: 0.842 ± 0.245
5.139GlyIle: 5.139 ± 0.622
4.128GlyLys: 4.128 ± 0.589
6.234GlyLeu: 6.234 ± 0.703
2.949GlyMet: 2.949 ± 0.488
4.549GlyAsn: 4.549 ± 0.736
1.516GlyPro: 1.516 ± 0.399
3.538GlyGln: 3.538 ± 0.552
4.634GlyArg: 4.634 ± 0.638
4.465GlySer: 4.465 ± 0.711
5.897GlyThr: 5.897 ± 0.919
5.476GlyVal: 5.476 ± 0.579
0.927GlyTrp: 0.927 ± 0.263
2.106GlyTyr: 2.106 ± 0.373
0.0GlyXaa: 0.0 ± 0.0
His
1.264HisAla: 1.264 ± 0.339
0.168HisCys: 0.168 ± 0.106
0.842HisAsp: 0.842 ± 0.266
0.927HisGlu: 0.927 ± 0.297
0.505HisPhe: 0.505 ± 0.192
1.432HisGly: 1.432 ± 0.381
0.337HisHis: 0.337 ± 0.183
0.674HisIle: 0.674 ± 0.282
1.011HisLys: 1.011 ± 0.378
1.601HisLeu: 1.601 ± 0.578
0.253HisMet: 0.253 ± 0.178
0.59HisAsn: 0.59 ± 0.21
1.179HisPro: 1.179 ± 0.351
0.758HisGln: 0.758 ± 0.313
1.432HisArg: 1.432 ± 0.297
1.264HisSer: 1.264 ± 0.373
0.59HisThr: 0.59 ± 0.188
0.59HisVal: 0.59 ± 0.235
0.168HisTrp: 0.168 ± 0.136
0.337HisTyr: 0.337 ± 0.163
0.0HisXaa: 0.0 ± 0.0
Ile
5.055IleAla: 5.055 ± 0.526
0.842IleCys: 0.842 ± 0.357
4.212IleAsp: 4.212 ± 0.563
3.286IleGlu: 3.286 ± 0.485
1.853IlePhe: 1.853 ± 0.359
4.044IleGly: 4.044 ± 0.75
1.264IleHis: 1.264 ± 0.378
3.033IleIle: 3.033 ± 0.473
2.359IleLys: 2.359 ± 0.425
4.971IleLeu: 4.971 ± 0.517
1.011IleMet: 1.011 ± 0.327
3.117IleAsn: 3.117 ± 0.539
2.275IlePro: 2.275 ± 0.444
2.612IleGln: 2.612 ± 0.617
4.044IleArg: 4.044 ± 0.639
4.549IleSer: 4.549 ± 0.625
4.297IleThr: 4.297 ± 0.596
3.286IleVal: 3.286 ± 0.512
0.59IleTrp: 0.59 ± 0.182
1.516IleTyr: 1.516 ± 0.275
0.0IleXaa: 0.0 ± 0.0
Lys
5.392LysAla: 5.392 ± 0.792
0.421LysCys: 0.421 ± 0.241
3.033LysAsp: 3.033 ± 0.633
3.875LysGlu: 3.875 ± 0.596
1.348LysPhe: 1.348 ± 0.404
3.454LysGly: 3.454 ± 0.508
0.674LysHis: 0.674 ± 0.283
2.443LysIle: 2.443 ± 0.51
4.128LysLys: 4.128 ± 0.972
3.875LysLeu: 3.875 ± 0.507
2.359LysMet: 2.359 ± 0.456
2.612LysAsn: 2.612 ± 0.538
3.623LysPro: 3.623 ± 0.832
2.275LysGln: 2.275 ± 0.5
2.527LysArg: 2.527 ± 0.658
3.623LysSer: 3.623 ± 0.664
3.201LysThr: 3.201 ± 0.424
3.454LysVal: 3.454 ± 0.528
1.011LysTrp: 1.011 ± 0.28
1.853LysTyr: 1.853 ± 0.36
0.0LysXaa: 0.0 ± 0.0
Leu
8.677LeuAla: 8.677 ± 0.998
0.842LeuCys: 0.842 ± 0.331
4.381LeuAsp: 4.381 ± 0.47
5.56LeuGlu: 5.56 ± 0.69
1.853LeuPhe: 1.853 ± 0.454
4.044LeuGly: 4.044 ± 0.553
1.179LeuHis: 1.179 ± 0.313
4.297LeuIle: 4.297 ± 0.491
4.634LeuLys: 4.634 ± 0.583
5.476LeuLeu: 5.476 ± 0.61
1.179LeuMet: 1.179 ± 0.347
4.802LeuAsn: 4.802 ± 0.575
3.201LeuPro: 3.201 ± 0.628
3.538LeuGln: 3.538 ± 0.52
5.644LeuArg: 5.644 ± 0.732
5.729LeuSer: 5.729 ± 0.64
5.476LeuThr: 5.476 ± 0.641
4.381LeuVal: 4.381 ± 0.649
0.59LeuTrp: 0.59 ± 0.269
2.443LeuTyr: 2.443 ± 0.429
0.0LeuXaa: 0.0 ± 0.0
Met
2.949MetAla: 2.949 ± 0.435
0.421MetCys: 0.421 ± 0.222
1.432MetAsp: 1.432 ± 0.26
1.348MetGlu: 1.348 ± 0.357
0.842MetPhe: 0.842 ± 0.259
2.022MetGly: 2.022 ± 0.337
0.253MetHis: 0.253 ± 0.124
1.095MetIle: 1.095 ± 0.377
1.601MetLys: 1.601 ± 0.314
2.864MetLeu: 2.864 ± 0.675
0.421MetMet: 0.421 ± 0.165
0.505MetAsn: 0.505 ± 0.217
1.179MetPro: 1.179 ± 0.323
1.348MetGln: 1.348 ± 0.33
1.432MetArg: 1.432 ± 0.342
2.443MetSer: 2.443 ± 0.471
1.938MetThr: 1.938 ± 0.464
0.927MetVal: 0.927 ± 0.248
0.168MetTrp: 0.168 ± 0.113
0.674MetTyr: 0.674 ± 0.221
0.0MetXaa: 0.0 ± 0.0
Asn
5.307AsnAla: 5.307 ± 0.496
0.674AsnCys: 0.674 ± 0.325
2.022AsnAsp: 2.022 ± 0.393
2.359AsnGlu: 2.359 ± 0.432
1.348AsnPhe: 1.348 ± 0.297
4.718AsnGly: 4.718 ± 0.683
1.011AsnHis: 1.011 ± 0.336
3.201AsnIle: 3.201 ± 0.457
2.275AsnLys: 2.275 ± 0.435
2.78AsnLeu: 2.78 ± 0.437
0.842AsnMet: 0.842 ± 0.215
2.527AsnAsn: 2.527 ± 0.478
2.527AsnPro: 2.527 ± 0.46
2.527AsnGln: 2.527 ± 0.42
2.106AsnArg: 2.106 ± 0.509
2.864AsnSer: 2.864 ± 0.59
3.538AsnThr: 3.538 ± 0.507
2.106AsnVal: 2.106 ± 0.463
1.011AsnTrp: 1.011 ± 0.205
1.516AsnTyr: 1.516 ± 0.421
0.0AsnXaa: 0.0 ± 0.0
Pro
3.454ProAla: 3.454 ± 0.627
0.421ProCys: 0.421 ± 0.203
2.19ProAsp: 2.19 ± 0.45
2.78ProGlu: 2.78 ± 0.539
1.179ProPhe: 1.179 ± 0.33
3.286ProGly: 3.286 ± 0.444
0.758ProHis: 0.758 ± 0.25
1.432ProIle: 1.432 ± 0.422
2.19ProLys: 2.19 ± 0.491
2.696ProLeu: 2.696 ± 0.779
1.348ProMet: 1.348 ± 0.333
1.179ProAsn: 1.179 ± 0.373
1.685ProPro: 1.685 ± 0.354
2.443ProGln: 2.443 ± 0.455
2.022ProArg: 2.022 ± 0.462
2.78ProSer: 2.78 ± 0.533
2.106ProThr: 2.106 ± 0.406
2.864ProVal: 2.864 ± 0.399
0.421ProTrp: 0.421 ± 0.207
0.927ProTyr: 0.927 ± 0.273
0.0ProXaa: 0.0 ± 0.0
Gln
5.56GlnAla: 5.56 ± 1.122
0.337GlnCys: 0.337 ± 0.167
1.516GlnAsp: 1.516 ± 0.291
2.359GlnGlu: 2.359 ± 0.464
1.264GlnPhe: 1.264 ± 0.326
2.443GlnGly: 2.443 ± 0.549
0.842GlnHis: 0.842 ± 0.26
2.864GlnIle: 2.864 ± 0.397
3.286GlnLys: 3.286 ± 0.478
3.623GlnLeu: 3.623 ± 0.44
1.095GlnMet: 1.095 ± 0.292
2.19GlnAsn: 2.19 ± 0.496
1.769GlnPro: 1.769 ± 0.411
3.286GlnGln: 3.286 ± 0.59
3.37GlnArg: 3.37 ± 0.719
3.37GlnSer: 3.37 ± 0.549
2.864GlnThr: 2.864 ± 0.557
3.454GlnVal: 3.454 ± 0.551
0.674GlnTrp: 0.674 ± 0.261
1.348GlnTyr: 1.348 ± 0.4
0.0GlnXaa: 0.0 ± 0.0
Arg
4.465ArgAla: 4.465 ± 0.68
0.59ArgCys: 0.59 ± 0.288
3.37ArgAsp: 3.37 ± 0.588
3.37ArgGlu: 3.37 ± 0.658
2.022ArgPhe: 2.022 ± 0.333
3.791ArgGly: 3.791 ± 0.588
1.348ArgHis: 1.348 ± 0.338
3.623ArgIle: 3.623 ± 0.471
4.465ArgLys: 4.465 ± 0.526
5.897ArgLeu: 5.897 ± 0.48
1.938ArgMet: 1.938 ± 0.414
3.117ArgAsn: 3.117 ± 0.483
1.516ArgPro: 1.516 ± 0.404
2.443ArgGln: 2.443 ± 0.383
3.623ArgArg: 3.623 ± 0.727
3.286ArgSer: 3.286 ± 0.523
3.623ArgThr: 3.623 ± 0.452
4.044ArgVal: 4.044 ± 0.579
1.516ArgTrp: 1.516 ± 0.384
2.022ArgTyr: 2.022 ± 0.397
0.0ArgXaa: 0.0 ± 0.0
Ser
5.813SerAla: 5.813 ± 0.851
0.505SerCys: 0.505 ± 0.186
4.128SerAsp: 4.128 ± 0.621
3.37SerGlu: 3.37 ± 0.446
2.78SerPhe: 2.78 ± 0.408
6.15SerGly: 6.15 ± 0.675
0.758SerHis: 0.758 ± 0.256
3.96SerIle: 3.96 ± 0.449
3.538SerLys: 3.538 ± 0.611
5.644SerLeu: 5.644 ± 0.786
1.516SerMet: 1.516 ± 0.337
2.949SerAsn: 2.949 ± 0.474
2.864SerPro: 2.864 ± 0.449
3.117SerGln: 3.117 ± 0.467
5.307SerArg: 5.307 ± 0.632
4.718SerSer: 4.718 ± 0.941
3.538SerThr: 3.538 ± 0.577
5.476SerVal: 5.476 ± 0.906
1.685SerTrp: 1.685 ± 0.272
1.348SerTyr: 1.348 ± 0.279
0.0SerXaa: 0.0 ± 0.0
Thr
6.908ThrAla: 6.908 ± 0.96
0.59ThrCys: 0.59 ± 0.185
3.623ThrAsp: 3.623 ± 0.507
3.538ThrGlu: 3.538 ± 0.518
2.527ThrPhe: 2.527 ± 0.628
5.56ThrGly: 5.56 ± 0.696
0.505ThrHis: 0.505 ± 0.188
3.538ThrIle: 3.538 ± 0.599
3.707ThrLys: 3.707 ± 0.541
3.707ThrLeu: 3.707 ± 0.475
1.179ThrMet: 1.179 ± 0.255
2.275ThrAsn: 2.275 ± 0.445
2.696ThrPro: 2.696 ± 0.641
2.612ThrGln: 2.612 ± 0.759
2.696ThrArg: 2.696 ± 0.41
4.465ThrSer: 4.465 ± 0.545
3.033ThrThr: 3.033 ± 0.54
4.381ThrVal: 4.381 ± 0.521
1.432ThrTrp: 1.432 ± 0.306
1.179ThrTyr: 1.179 ± 0.349
0.0ThrXaa: 0.0 ± 0.0
Val
5.055ValAla: 5.055 ± 0.733
0.505ValCys: 0.505 ± 0.237
3.033ValAsp: 3.033 ± 0.371
3.791ValGlu: 3.791 ± 0.481
2.022ValPhe: 2.022 ± 0.324
5.476ValGly: 5.476 ± 0.8
1.095ValHis: 1.095 ± 0.288
4.381ValIle: 4.381 ± 0.655
3.454ValLys: 3.454 ± 0.496
4.718ValLeu: 4.718 ± 0.635
1.769ValMet: 1.769 ± 0.346
2.78ValAsn: 2.78 ± 0.477
2.359ValPro: 2.359 ± 0.377
2.696ValGln: 2.696 ± 0.39
3.875ValArg: 3.875 ± 0.578
5.223ValSer: 5.223 ± 0.824
3.707ValThr: 3.707 ± 0.617
4.886ValVal: 4.886 ± 0.715
1.095ValTrp: 1.095 ± 0.335
2.19ValTyr: 2.19 ± 0.424
0.0ValXaa: 0.0 ± 0.0
Trp
1.179TrpAla: 1.179 ± 0.309
0.421TrpCys: 0.421 ± 0.179
1.011TrpAsp: 1.011 ± 0.263
0.758TrpGlu: 0.758 ± 0.206
0.421TrpPhe: 0.421 ± 0.181
1.264TrpGly: 1.264 ± 0.343
0.674TrpHis: 0.674 ± 0.22
0.59TrpIle: 0.59 ± 0.2
1.011TrpLys: 1.011 ± 0.34
1.432TrpLeu: 1.432 ± 0.386
0.59TrpMet: 0.59 ± 0.225
0.505TrpAsn: 0.505 ± 0.238
0.59TrpPro: 0.59 ± 0.168
0.505TrpGln: 0.505 ± 0.182
1.095TrpArg: 1.095 ± 0.325
0.758TrpSer: 0.758 ± 0.266
1.179TrpThr: 1.179 ± 0.356
1.179TrpVal: 1.179 ± 0.289
0.421TrpTrp: 0.421 ± 0.185
0.59TrpTyr: 0.59 ± 0.21
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.201TyrAla: 3.201 ± 0.516
0.421TyrCys: 0.421 ± 0.182
1.685TyrAsp: 1.685 ± 0.31
1.685TyrGlu: 1.685 ± 0.522
0.59TyrPhe: 0.59 ± 0.25
2.275TyrGly: 2.275 ± 0.393
0.421TyrHis: 0.421 ± 0.185
2.022TyrIle: 2.022 ± 0.311
0.758TyrLys: 0.758 ± 0.236
1.769TyrLeu: 1.769 ± 0.441
0.337TyrMet: 0.337 ± 0.169
1.095TyrAsn: 1.095 ± 0.285
1.348TyrPro: 1.348 ± 0.444
1.685TyrGln: 1.685 ± 0.323
2.864TyrArg: 2.864 ± 0.551
2.275TyrSer: 2.275 ± 0.503
1.264TyrThr: 1.264 ± 0.421
1.348TyrVal: 1.348 ± 0.271
0.421TyrTrp: 0.421 ± 0.174
0.758TyrTyr: 0.758 ± 0.266
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 57 proteins (11871 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski