candidate division MSBL1 archaeon SCGC-AAA259E17

Taxonomy: cellular organisms; Archaea; Euryarchaeota; Euryarchaeota incertae sedis; candidate division MSBL1

Average proteome isoelectric point is 6.2

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 891 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A133UFQ5|A0A133UFQ5_9EURY DEDD_Tnp_IS110 domain-containing protein OS=candidate division MSBL1 archaeon SCGC-AAA259E17 OX=1698263 GN=AKJ64_01495 PE=4 SV=1
MM1 pKa = 7.51GFGAALGVALGEE13 pKa = 4.42DD14 pKa = 3.12AGEE17 pKa = 4.21TVRR20 pKa = 11.84IADD23 pKa = 4.25AEE25 pKa = 4.66DD26 pKa = 3.18ISGGVTAEE34 pKa = 3.98LACYY38 pKa = 10.02DD39 pKa = 3.42RR40 pKa = 11.84WTLLIHH46 pKa = 5.6VAGAIDD52 pKa = 3.69VTVEE56 pKa = 3.94LSPDD60 pKa = 3.77GKK62 pKa = 9.87ATWYY66 pKa = 10.32EE67 pKa = 3.92IDD69 pKa = 3.91EE70 pKa = 4.44SPYY73 pKa = 10.86SYY75 pKa = 11.05GAAGDD80 pKa = 3.92STNEE84 pKa = 3.73LGYY87 pKa = 10.7DD88 pKa = 3.31ATHH91 pKa = 6.73IRR93 pKa = 11.84LTGSNATSVTAQIRR107 pKa = 11.84GVWW110 pKa = 3.13

Molecular weight: 11.47 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A133UFL5|A0A133UFL5_9EURY Uncharacterized protein OS=candidate division MSBL1 archaeon SCGC-AAA259E17 OX=1698263 GN=AKJ64_01650 PE=4 SV=1
MM1 pKa = 7.72ISILIAYY8 pKa = 7.24PLTVGVVSVIVGFRR22 pKa = 11.84HH23 pKa = 6.23LFKK26 pKa = 10.59HH27 pKa = 5.62RR28 pKa = 11.84HH29 pKa = 4.82LHH31 pKa = 5.11LQQLGEE37 pKa = 4.03LHH39 pKa = 6.68ALRR42 pKa = 11.84LFRR45 pKa = 11.84HH46 pKa = 6.51CDD48 pKa = 3.39VVPDD52 pKa = 4.13DD53 pKa = 5.48FIDD56 pKa = 4.3GVARR60 pKa = 11.84SSTLHH65 pKa = 5.42SHH67 pKa = 6.12LQIGQLFLIGFRR79 pKa = 11.84SQSPRR84 pKa = 11.84RR85 pKa = 11.84LLVPRR90 pKa = 11.84FPDD93 pKa = 3.05IQQMFKK99 pKa = 10.41PIEE102 pKa = 3.79HH103 pKa = 6.5SRR105 pKa = 11.84RR106 pKa = 11.84ILRR109 pKa = 11.84DD110 pKa = 3.07GLYY113 pKa = 10.09SEE115 pKa = 5.1KK116 pKa = 10.65LQEE119 pKa = 3.96ILKK122 pKa = 10.53LILNIAWGSAVSGYY136 pKa = 9.57LLKK139 pKa = 10.68RR140 pKa = 11.84RR141 pKa = 11.84LLL143 pKa = 3.93

Molecular weight: 16.55 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

891

0

891

190103

52

1524

213.4

24.12

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.927 ± 0.099

0.991 ± 0.037

5.827 ± 0.086

10.646 ± 0.123

3.874 ± 0.065

7.445 ± 0.086

1.711 ± 0.029

6.41 ± 0.076

7.298 ± 0.095

9.218 ± 0.09

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.197 ± 0.05

3.642 ± 0.063

4.216 ± 0.057

2.255 ± 0.037

6.245 ± 0.083

6.591 ± 0.077

4.57 ± 0.059

6.736 ± 0.087

1.265 ± 0.033

2.938 ± 0.052
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski