Dyadobacter luteus

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Cytophagia; Cytophagales; Cytophagaceae; Dyadobacter

Average proteome isoelectric point is 6.71

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5642 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3D8Y7G2|A0A3D8Y7G2_9BACT DUF4180 domain-containing protein OS=Dyadobacter luteus OX=2259619 GN=DSL64_19730 PE=4 SV=1
MM1 pKa = 7.66NYY3 pKa = 9.46TYY5 pKa = 11.24KK6 pKa = 10.7LRR8 pKa = 11.84LLPEE12 pKa = 4.11SDD14 pKa = 2.78GGYY17 pKa = 8.36TVFVPALPGCITYY30 pKa = 10.95GEE32 pKa = 4.69TIDD35 pKa = 4.85EE36 pKa = 5.05AIEE39 pKa = 3.9MAKK42 pKa = 10.16EE43 pKa = 4.12AISLYY48 pKa = 11.0VEE50 pKa = 4.79DD51 pKa = 4.04LTDD54 pKa = 3.92RR55 pKa = 11.84GEE57 pKa = 4.8PIPDD61 pKa = 3.82DD62 pKa = 4.25SSTLEE67 pKa = 3.73YY68 pKa = 11.02SLNLVAA74 pKa = 6.27

Molecular weight: 8.18 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3D8YFX2|A0A3D8YFX2_9BACT DNA-binding response regulator OS=Dyadobacter luteus OX=2259619 GN=DSL64_03615 PE=4 SV=1
MM1 pKa = 7.61LNQFRR6 pKa = 11.84IRR8 pKa = 11.84YY9 pKa = 7.4NNRR12 pKa = 11.84KK13 pKa = 9.45AIQCRR18 pKa = 11.84RR19 pKa = 11.84HH20 pKa = 5.92PGIAVADD27 pKa = 3.96RR28 pKa = 11.84LVIRR32 pKa = 11.84FAGKK36 pKa = 8.98PRR38 pKa = 11.84NDD40 pKa = 2.92IDD42 pKa = 3.71RR43 pKa = 11.84RR44 pKa = 11.84IRR46 pKa = 11.84KK47 pKa = 6.63YY48 pKa = 8.34TNGYY52 pKa = 9.1IDD54 pKa = 3.99MNIHH58 pKa = 6.19RR59 pKa = 11.84PSLIGVASAFLWRR72 pKa = 11.84CNSDD76 pKa = 3.71EE77 pKa = 4.78IIPAAEE83 pKa = 3.78EE84 pKa = 4.18LFPVEE89 pKa = 4.06YY90 pKa = 10.23TFVNASANQLHH101 pKa = 5.62TVNLTAQTHH110 pKa = 5.41FPKK113 pKa = 10.63EE114 pKa = 3.6QTTNIRR120 pKa = 3.43

Molecular weight: 13.97 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5642

0

5642

2023372

25

2408

358.6

40.13

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.474 ± 0.032

0.709 ± 0.01

5.32 ± 0.02

5.839 ± 0.032

4.864 ± 0.021

7.076 ± 0.032

1.849 ± 0.015

6.811 ± 0.029

6.418 ± 0.034

9.506 ± 0.03

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.237 ± 0.012

5.219 ± 0.029

3.899 ± 0.016

3.848 ± 0.018

4.417 ± 0.02

6.857 ± 0.027

5.806 ± 0.026

6.624 ± 0.023

1.264 ± 0.014

3.964 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski