Amino acid dipepetide frequency for Roseburia sp. AM16-25

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
6.694AlaAla: 6.694 ± 0.132
1.216AlaCys: 1.216 ± 0.044
5.287AlaAsp: 5.287 ± 0.093
5.561AlaGlu: 5.561 ± 0.092
3.141AlaPhe: 3.141 ± 0.071
6.185AlaGly: 6.185 ± 0.101
1.324AlaHis: 1.324 ± 0.045
5.673AlaIle: 5.673 ± 0.099
5.67AlaLys: 5.67 ± 0.091
7.104AlaLeu: 7.104 ± 0.118
2.828AlaMet: 2.828 ± 0.068
2.729AlaAsn: 2.729 ± 0.062
2.071AlaPro: 2.071 ± 0.056
2.571AlaGln: 2.571 ± 0.055
2.99AlaArg: 2.99 ± 0.061
4.489AlaSer: 4.489 ± 0.074
3.895AlaThr: 3.895 ± 0.073
5.711AlaVal: 5.711 ± 0.087
0.665AlaTrp: 0.665 ± 0.028
3.312AlaTyr: 3.312 ± 0.066
0.0AlaXaa: 0.0 ± 0.0
Cys
1.058CysAla: 1.058 ± 0.034
0.234CysCys: 0.234 ± 0.016
0.869CysAsp: 0.869 ± 0.031
0.802CysGlu: 0.802 ± 0.036
0.616CysPhe: 0.616 ± 0.029
1.436CysGly: 1.436 ± 0.047
0.363CysHis: 0.363 ± 0.022
1.131CysIle: 1.131 ± 0.037
0.858CysLys: 0.858 ± 0.035
1.22CysLeu: 1.22 ± 0.042
0.528CysMet: 0.528 ± 0.027
0.638CysAsn: 0.638 ± 0.026
0.616CysPro: 0.616 ± 0.033
0.597CysGln: 0.597 ± 0.029
0.635CysArg: 0.635 ± 0.028
0.812CysSer: 0.812 ± 0.032
0.637CysThr: 0.637 ± 0.031
0.993CysVal: 0.993 ± 0.038
0.127CysTrp: 0.127 ± 0.014
0.617CysTyr: 0.617 ± 0.029
0.0CysXaa: 0.0 ± 0.0
Asp
5.442AspAla: 5.442 ± 0.091
0.848AspCys: 0.848 ± 0.033
3.377AspAsp: 3.377 ± 0.082
4.622AspGlu: 4.622 ± 0.086
2.752AspPhe: 2.752 ± 0.067
4.594AspGly: 4.594 ± 0.085
1.185AspHis: 1.185 ± 0.037
4.553AspIle: 4.553 ± 0.08
3.679AspLys: 3.679 ± 0.076
5.324AspLeu: 5.324 ± 0.081
2.138AspMet: 2.138 ± 0.051
2.211AspAsn: 2.211 ± 0.053
1.946AspPro: 1.946 ± 0.06
1.893AspGln: 1.893 ± 0.055
2.522AspArg: 2.522 ± 0.061
2.826AspSer: 2.826 ± 0.068
3.23AspThr: 3.23 ± 0.071
4.217AspVal: 4.217 ± 0.076
0.623AspTrp: 0.623 ± 0.03
3.166AspTyr: 3.166 ± 0.067
0.0AspXaa: 0.0 ± 0.0
Glu
5.749GluAla: 5.749 ± 0.097
0.775GluCys: 0.775 ± 0.031
4.93GluAsp: 4.93 ± 0.086
6.659GluGlu: 6.659 ± 0.122
2.255GluPhe: 2.255 ± 0.055
4.164GluGly: 4.164 ± 0.068
1.509GluHis: 1.509 ± 0.047
5.346GluIle: 5.346 ± 0.086
6.285GluLys: 6.285 ± 0.1
5.905GluLeu: 5.905 ± 0.101
2.512GluMet: 2.512 ± 0.056
3.782GluAsn: 3.782 ± 0.074
1.782GluPro: 1.782 ± 0.048
2.945GluGln: 2.945 ± 0.072
3.265GluArg: 3.265 ± 0.075
3.134GluSer: 3.134 ± 0.064
3.637GluThr: 3.637 ± 0.076
4.607GluVal: 4.607 ± 0.08
0.601GluTrp: 0.601 ± 0.029
2.807GluTyr: 2.807 ± 0.069
0.0GluXaa: 0.0 ± 0.0
Phe
3.448PheAla: 3.448 ± 0.077
0.756PheCys: 0.756 ± 0.034
2.549PheAsp: 2.549 ± 0.061
2.302PheGlu: 2.302 ± 0.046
1.976PhePhe: 1.976 ± 0.057
3.002PheGly: 3.002 ± 0.068
0.851PheHis: 0.851 ± 0.031
2.489PheIle: 2.489 ± 0.061
1.677PheLys: 1.677 ± 0.048
3.86PheLeu: 3.86 ± 0.088
1.251PheMet: 1.251 ± 0.038
1.283PheAsn: 1.283 ± 0.043
1.293PhePro: 1.293 ± 0.047
1.132PheGln: 1.132 ± 0.035
1.434PheArg: 1.434 ± 0.043
2.982PheSer: 2.982 ± 0.067
2.223PheThr: 2.223 ± 0.059
3.154PheVal: 3.154 ± 0.066
0.44PheTrp: 0.44 ± 0.025
1.757PheTyr: 1.757 ± 0.044
0.0PheXaa: 0.0 ± 0.0
Gly
4.928GlyAla: 4.928 ± 0.094
1.192GlyCys: 1.192 ± 0.04
3.784GlyAsp: 3.784 ± 0.065
4.24GlyGlu: 4.24 ± 0.07
3.064GlyPhe: 3.064 ± 0.067
4.545GlyGly: 4.545 ± 0.097
1.342GlyHis: 1.342 ± 0.045
5.991GlyIle: 5.991 ± 0.107
5.471GlyLys: 5.471 ± 0.087
5.954GlyLeu: 5.954 ± 0.095
2.767GlyMet: 2.767 ± 0.058
3.111GlyAsn: 3.111 ± 0.08
1.199GlyPro: 1.199 ± 0.039
2.714GlyGln: 2.714 ± 0.065
3.081GlyArg: 3.081 ± 0.073
4.034GlySer: 4.034 ± 0.077
3.91GlyThr: 3.91 ± 0.08
4.793GlyVal: 4.793 ± 0.096
0.733GlyTrp: 0.733 ± 0.036
3.38GlyTyr: 3.38 ± 0.058
0.0GlyXaa: 0.0 ± 0.0
His
1.339HisAla: 1.339 ± 0.042
0.31HisCys: 0.31 ± 0.02
0.999HisAsp: 0.999 ± 0.034
1.209HisGlu: 1.209 ± 0.045
0.981HisPhe: 0.981 ± 0.04
1.406HisGly: 1.406 ± 0.044
0.474HisHis: 0.474 ± 0.029
1.435HisIle: 1.435 ± 0.045
1.092HisLys: 1.092 ± 0.04
1.795HisLeu: 1.795 ± 0.048
0.741HisMet: 0.741 ± 0.033
0.716HisAsn: 0.716 ± 0.029
0.965HisPro: 0.965 ± 0.037
0.616HisGln: 0.616 ± 0.029
0.842HisArg: 0.842 ± 0.038
0.945HisSer: 0.945 ± 0.036
1.064HisThr: 1.064 ± 0.035
1.28HisVal: 1.28 ± 0.05
0.151HisTrp: 0.151 ± 0.016
0.925HisTyr: 0.925 ± 0.035
0.0HisXaa: 0.0 ± 0.0
Ile
6.26IleAla: 6.26 ± 0.087
1.364IleCys: 1.364 ± 0.038
4.217IleAsp: 4.217 ± 0.071
4.567IleGlu: 4.567 ± 0.099
3.003IlePhe: 3.003 ± 0.071
5.166IleGly: 5.166 ± 0.081
1.361IleHis: 1.361 ± 0.041
4.607IleIle: 4.607 ± 0.089
3.755IleLys: 3.755 ± 0.088
6.909IleLeu: 6.909 ± 0.116
2.11IleMet: 2.11 ± 0.054
2.612IleAsn: 2.612 ± 0.065
2.802IlePro: 2.802 ± 0.064
2.052IleGln: 2.052 ± 0.05
3.405IleArg: 3.405 ± 0.065
4.768IleSer: 4.768 ± 0.079
4.193IleThr: 4.193 ± 0.084
5.133IleVal: 5.133 ± 0.09
0.623IleTrp: 0.623 ± 0.031
2.899IleTyr: 2.899 ± 0.07
0.0IleXaa: 0.0 ± 0.0
Lys
4.717LysAla: 4.717 ± 0.084
0.705LysCys: 0.705 ± 0.03
4.207LysAsp: 4.207 ± 0.081
6.575LysGlu: 6.575 ± 0.1
1.706LysPhe: 1.706 ± 0.044
3.745LysGly: 3.745 ± 0.074
1.136LysHis: 1.136 ± 0.038
4.283LysIle: 4.283 ± 0.073
6.055LysLys: 6.055 ± 0.112
4.514LysLeu: 4.514 ± 0.069
2.275LysMet: 2.275 ± 0.053
3.249LysAsn: 3.249 ± 0.069
1.785LysPro: 1.785 ± 0.044
2.508LysGln: 2.508 ± 0.054
3.152LysArg: 3.152 ± 0.063
3.007LysSer: 3.007 ± 0.064
3.792LysThr: 3.792 ± 0.071
4.131LysVal: 4.131 ± 0.076
0.57LysTrp: 0.57 ± 0.028
2.793LysTyr: 2.793 ± 0.068
0.0LysXaa: 0.0 ± 0.0
Leu
7.361LeuAla: 7.361 ± 0.114
1.651LeuCys: 1.651 ± 0.05
5.35LeuAsp: 5.35 ± 0.096
5.798LeuGlu: 5.798 ± 0.102
3.664LeuPhe: 3.664 ± 0.086
6.05LeuGly: 6.05 ± 0.106
1.806LeuHis: 1.806 ± 0.049
5.658LeuIle: 5.658 ± 0.085
4.776LeuLys: 4.776 ± 0.073
8.989LeuLeu: 8.989 ± 0.156
2.68LeuMet: 2.68 ± 0.059
3.351LeuAsn: 3.351 ± 0.056
3.5LeuPro: 3.5 ± 0.067
3.489LeuGln: 3.489 ± 0.07
3.818LeuArg: 3.818 ± 0.075
6.008LeuSer: 6.008 ± 0.094
4.911LeuThr: 4.911 ± 0.08
5.942LeuVal: 5.942 ± 0.092
0.888LeuTrp: 0.888 ± 0.036
3.438LeuTyr: 3.438 ± 0.083
0.0LeuXaa: 0.0 ± 0.0
Met
2.84MetAla: 2.84 ± 0.07
0.381MetCys: 0.381 ± 0.022
2.255MetAsp: 2.255 ± 0.053
2.813MetGlu: 2.813 ± 0.062
1.063MetPhe: 1.063 ± 0.04
2.309MetGly: 2.309 ± 0.066
0.573MetHis: 0.573 ± 0.026
2.312MetIle: 2.312 ± 0.059
2.327MetLys: 2.327 ± 0.055
2.797MetLeu: 2.797 ± 0.065
1.128MetMet: 1.128 ± 0.041
1.455MetAsn: 1.455 ± 0.043
1.284MetPro: 1.284 ± 0.04
1.527MetGln: 1.527 ± 0.052
1.478MetArg: 1.478 ± 0.04
1.847MetSer: 1.847 ± 0.051
1.932MetThr: 1.932 ± 0.058
2.208MetVal: 2.208 ± 0.045
0.231MetTrp: 0.231 ± 0.019
0.97MetTyr: 0.97 ± 0.039
0.0MetXaa: 0.0 ± 0.0
Asn
3.262AsnAla: 3.262 ± 0.074
0.549AsnCys: 0.549 ± 0.025
2.219AsnAsp: 2.219 ± 0.05
2.527AsnGlu: 2.527 ± 0.066
1.42AsnPhe: 1.42 ± 0.042
3.19AsnGly: 3.19 ± 0.073
0.891AsnHis: 0.891 ± 0.035
3.178AsnIle: 3.178 ± 0.08
2.16AsnLys: 2.16 ± 0.053
3.568AsnLeu: 3.568 ± 0.065
1.359AsnMet: 1.359 ± 0.042
1.643AsnAsn: 1.643 ± 0.057
1.76AsnPro: 1.76 ± 0.053
1.514AsnGln: 1.514 ± 0.041
1.953AsnArg: 1.953 ± 0.05
1.846AsnSer: 1.846 ± 0.052
2.235AsnThr: 2.235 ± 0.059
2.949AsnVal: 2.949 ± 0.058
0.42AsnTrp: 0.42 ± 0.025
1.823AsnTyr: 1.823 ± 0.057
0.0AsnXaa: 0.0 ± 0.0
Pro
2.285ProAla: 2.285 ± 0.061
0.426ProCys: 0.426 ± 0.023
2.118ProAsp: 2.118 ± 0.06
2.77ProGlu: 2.77 ± 0.067
1.429ProPhe: 1.429 ± 0.044
2.14ProGly: 2.14 ± 0.06
0.599ProHis: 0.599 ± 0.031
2.279ProIle: 2.279 ± 0.06
1.989ProLys: 1.989 ± 0.051
2.777ProLeu: 2.777 ± 0.064
0.99ProMet: 0.99 ± 0.036
1.196ProAsn: 1.196 ± 0.044
0.631ProPro: 0.631 ± 0.027
1.209ProGln: 1.209 ± 0.048
1.013ProArg: 1.013 ± 0.04
1.699ProSer: 1.699 ± 0.049
1.675ProThr: 1.675 ± 0.049
2.582ProVal: 2.582 ± 0.054
0.335ProTrp: 0.335 ± 0.023
1.501ProTyr: 1.501 ± 0.043
0.0ProXaa: 0.0 ± 0.0
Gln
3.023GlnAla: 3.023 ± 0.071
0.363GlnCys: 0.363 ± 0.023
2.058GlnAsp: 2.058 ± 0.056
3.064GlnGlu: 3.064 ± 0.066
1.22GlnPhe: 1.22 ± 0.037
2.265GlnGly: 2.265 ± 0.051
0.554GlnHis: 0.554 ± 0.026
2.948GlnIle: 2.948 ± 0.065
2.877GlnLys: 2.877 ± 0.063
2.796GlnLeu: 2.796 ± 0.061
1.491GlnMet: 1.491 ± 0.042
1.622GlnAsn: 1.622 ± 0.044
1.016GlnPro: 1.016 ± 0.039
1.251GlnGln: 1.251 ± 0.044
1.403GlnArg: 1.403 ± 0.046
1.679GlnSer: 1.679 ± 0.047
2.083GlnThr: 2.083 ± 0.054
2.539GlnVal: 2.539 ± 0.06
0.319GlnTrp: 0.319 ± 0.022
1.336GlnTyr: 1.336 ± 0.04
0.0GlnXaa: 0.0 ± 0.0
Arg
2.735ArgAla: 2.735 ± 0.06
0.539ArgCys: 0.539 ± 0.029
2.353ArgAsp: 2.353 ± 0.055
3.293ArgGlu: 3.293 ± 0.074
1.734ArgPhe: 1.734 ± 0.044
2.456ArgGly: 2.456 ± 0.055
0.858ArgHis: 0.858 ± 0.033
3.414ArgIle: 3.414 ± 0.06
3.152ArgLys: 3.152 ± 0.072
3.836ArgLeu: 3.836 ± 0.077
1.641ArgMet: 1.641 ± 0.044
1.777ArgAsn: 1.777 ± 0.05
1.376ArgPro: 1.376 ± 0.049
2.032ArgGln: 2.032 ± 0.049
2.234ArgArg: 2.234 ± 0.056
2.247ArgSer: 2.247 ± 0.052
2.107ArgThr: 2.107 ± 0.053
2.718ArgVal: 2.718 ± 0.061
0.372ArgTrp: 0.372 ± 0.023
1.885ArgTyr: 1.885 ± 0.051
0.0ArgXaa: 0.0 ± 0.0
Ser
4.35SerAla: 4.35 ± 0.077
0.734SerCys: 0.734 ± 0.033
3.44SerAsp: 3.44 ± 0.084
3.39SerGlu: 3.39 ± 0.07
2.502SerPhe: 2.502 ± 0.066
4.651SerGly: 4.651 ± 0.085
1.117SerHis: 1.117 ± 0.037
3.975SerIle: 3.975 ± 0.086
3.14SerLys: 3.14 ± 0.064
5.28SerLeu: 5.28 ± 0.083
1.932SerMet: 1.932 ± 0.049
2.166SerAsn: 2.166 ± 0.065
1.582SerPro: 1.582 ± 0.046
1.951SerGln: 1.951 ± 0.056
2.405SerArg: 2.405 ± 0.059
3.348SerSer: 3.348 ± 0.082
2.739SerThr: 2.739 ± 0.063
4.222SerVal: 4.222 ± 0.082
0.485SerTrp: 0.485 ± 0.023
2.69SerTyr: 2.69 ± 0.06
0.0SerXaa: 0.0 ± 0.0
Thr
4.135ThrAla: 4.135 ± 0.079
0.697ThrCys: 0.697 ± 0.033
3.389ThrAsp: 3.389 ± 0.068
3.704ThrGlu: 3.704 ± 0.07
2.302ThrPhe: 2.302 ± 0.059
4.415ThrGly: 4.415 ± 0.1
0.939ThrHis: 0.939 ± 0.036
4.081ThrIle: 4.081 ± 0.071
3.235ThrLys: 3.235 ± 0.065
4.88ThrLeu: 4.88 ± 0.075
1.622ThrMet: 1.622 ± 0.052
2.076ThrAsn: 2.076 ± 0.051
2.047ThrPro: 2.047 ± 0.051
1.641ThrGln: 1.641 ± 0.045
1.932ThrArg: 1.932 ± 0.043
3.179ThrSer: 3.179 ± 0.069
3.087ThrThr: 3.087 ± 0.078
3.903ThrVal: 3.903 ± 0.084
0.508ThrTrp: 0.508 ± 0.029
2.534ThrTyr: 2.534 ± 0.074
0.0ThrXaa: 0.0 ± 0.0
Val
5.641ValAla: 5.641 ± 0.091
1.297ValCys: 1.297 ± 0.041
4.248ValAsp: 4.248 ± 0.083
4.977ValGlu: 4.977 ± 0.086
2.832ValPhe: 2.832 ± 0.076
4.594ValGly: 4.594 ± 0.085
1.158ValHis: 1.158 ± 0.036
4.969ValIle: 4.969 ± 0.081
3.892ValLys: 3.892 ± 0.075
6.686ValLeu: 6.686 ± 0.097
2.159ValMet: 2.159 ± 0.057
2.655ValAsn: 2.655 ± 0.054
2.37ValPro: 2.37 ± 0.065
2.004ValGln: 2.004 ± 0.048
2.818ValArg: 2.818 ± 0.062
4.519ValSer: 4.519 ± 0.084
4.225ValThr: 4.225 ± 0.074
5.329ValVal: 5.329 ± 0.094
0.671ValTrp: 0.671 ± 0.03
2.813ValTyr: 2.813 ± 0.058
0.0ValXaa: 0.0 ± 0.0
Trp
0.58TrpAla: 0.58 ± 0.024
0.141TrpCys: 0.141 ± 0.013
0.569TrpAsp: 0.569 ± 0.026
0.616TrpGlu: 0.616 ± 0.028
0.354TrpPhe: 0.354 ± 0.025
0.712TrpGly: 0.712 ± 0.027
0.187TrpHis: 0.187 ± 0.016
0.684TrpIle: 0.684 ± 0.031
0.724TrpLys: 0.724 ± 0.032
0.91TrpLeu: 0.91 ± 0.036
0.342TrpMet: 0.342 ± 0.023
0.519TrpAsn: 0.519 ± 0.028
0.19TrpPro: 0.19 ± 0.016
0.442TrpGln: 0.442 ± 0.022
0.338TrpArg: 0.338 ± 0.022
0.501TrpSer: 0.501 ± 0.024
0.406TrpThr: 0.406 ± 0.023
0.509TrpVal: 0.509 ± 0.024
0.127TrpTrp: 0.127 ± 0.014
0.408TrpTyr: 0.408 ± 0.025
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.271TyrAla: 3.271 ± 0.074
0.606TyrCys: 0.606 ± 0.028
2.929TyrAsp: 2.929 ± 0.062
3.16TyrGlu: 3.16 ± 0.068
1.843TyrPhe: 1.843 ± 0.052
3.206TyrGly: 3.206 ± 0.07
1.059TyrHis: 1.059 ± 0.044
2.801TyrIle: 2.801 ± 0.065
2.092TyrLys: 2.092 ± 0.061
4.026TyrLeu: 4.026 ± 0.077
1.233TyrMet: 1.233 ± 0.042
1.674TyrAsn: 1.674 ± 0.056
1.43TyrPro: 1.43 ± 0.049
1.822TyrGln: 1.822 ± 0.05
2.04TyrArg: 2.04 ± 0.048
2.23TyrSer: 2.23 ± 0.063
2.322TyrThr: 2.322 ± 0.092
2.94TyrVal: 2.94 ± 0.059
0.371TyrTrp: 0.371 ± 0.021
2.01TyrTyr: 2.01 ± 0.075
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 2357 proteins (795901 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski