Escherichia phage vB_EcoM_ECO1230-10

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Jilinvirus; Escherichia virus CVM10

Average proteome isoelectric point is 6.37

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 56 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|D5LGZ4|D5LGZ4_9CAUD Putative phage tail protein OS=Escherichia phage vB_EcoM_ECO1230-10 OX=669875 PE=4 SV=1
MM1 pKa = 7.63AGAFTVVNLSQLPAPNVVEE20 pKa = 4.34SLDD23 pKa = 3.7FEE25 pKa = 4.97TILAAMIADD34 pKa = 3.83LQEE37 pKa = 4.5RR38 pKa = 11.84DD39 pKa = 3.64PTFTALVEE47 pKa = 4.06SDD49 pKa = 3.4PAYY52 pKa = 10.02KK53 pKa = 10.44VLEE56 pKa = 4.15VCAYY60 pKa = 10.29RR61 pKa = 11.84EE62 pKa = 3.67LLMRR66 pKa = 11.84HH67 pKa = 5.55RR68 pKa = 11.84VNEE71 pKa = 3.8AAKK74 pKa = 10.63AIMLAYY80 pKa = 9.1ATGSDD85 pKa = 4.46LDD87 pKa = 3.64QLGANVNVEE96 pKa = 4.2RR97 pKa = 11.84QTITPADD104 pKa = 4.25PDD106 pKa = 3.79AVPPVAAVMEE116 pKa = 4.53SDD118 pKa = 3.33DD119 pKa = 4.35DD120 pKa = 3.81FRR122 pKa = 11.84ARR124 pKa = 11.84IQLSPEE130 pKa = 4.08AYY132 pKa = 5.95TTAGSEE138 pKa = 4.13GSYY141 pKa = 10.56VFHH144 pKa = 7.58ALGADD149 pKa = 3.69PDD151 pKa = 4.23VKK153 pKa = 10.55DD154 pKa = 3.94VQPVSPTPGVVVVYY168 pKa = 9.86VLSRR172 pKa = 11.84TGDD175 pKa = 3.3GTAPEE180 pKa = 4.21SLLEE184 pKa = 4.17NVNTTLNAEE193 pKa = 4.67TVRR196 pKa = 11.84PMTDD200 pKa = 2.68QVQVQSAIIVPYY212 pKa = 10.3NIEE215 pKa = 4.31AEE217 pKa = 4.26LTLYY221 pKa = 9.49PGPDD225 pKa = 3.13ATVVLQSAIDD235 pKa = 3.56AVTAYY240 pKa = 10.6AEE242 pKa = 4.08TQRR245 pKa = 11.84RR246 pKa = 11.84IGYY249 pKa = 9.54DD250 pKa = 3.14VTLSGLYY257 pKa = 9.72HH258 pKa = 7.26ALHH261 pKa = 6.05QPGVQNVNLISPTEE275 pKa = 3.99SLVIGDD281 pKa = 4.57GQASYY286 pKa = 8.98CTSIAVNAAGEE297 pKa = 4.27TDD299 pKa = 3.39VV300 pKa = 4.74

Molecular weight: 31.92 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|D5LH09|D5LH09_9CAUD Putative phage holin OS=Escherichia phage vB_EcoM_ECO1230-10 OX=669875 PE=4 SV=1
MM1 pKa = 6.53TTFAINFIAVCFIFIWASWCVVYY24 pKa = 10.87SGVKK28 pKa = 10.22DD29 pKa = 4.13GIVGKK34 pKa = 10.49LFFSLVAISALAIIIHH50 pKa = 7.29TITGHH55 pKa = 5.03YY56 pKa = 7.93SQRR59 pKa = 11.84PFVTMNVSIALVGARR74 pKa = 11.84HH75 pKa = 6.12VFLSWLKK82 pKa = 10.12RR83 pKa = 11.84FKK85 pKa = 9.73TAHH88 pKa = 5.24EE89 pKa = 4.68RR90 pKa = 11.84YY91 pKa = 9.76

Molecular weight: 10.3 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

56

0

56

12865

50

825

229.7

25.23

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.525 ± 0.493

1.088 ± 0.142

6.172 ± 0.234

6.141 ± 0.37

3.202 ± 0.162

8.169 ± 0.277

1.617 ± 0.198

5.441 ± 0.252

5.192 ± 0.324

7.594 ± 0.194

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.581 ± 0.143

4.252 ± 0.27

4.493 ± 0.262

4.135 ± 0.2

6.032 ± 0.287

5.534 ± 0.32

6.592 ± 0.32

6.879 ± 0.307

1.718 ± 0.14

2.643 ± 0.206
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski