Amino acid dipepetide frequency for Escherichia phage HK544

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
11.25AlaAla: 11.25 ± 1.541
0.821AlaCys: 0.821 ± 0.333
7.637AlaAsp: 7.637 ± 0.754
7.472AlaGlu: 7.472 ± 1.663
3.202AlaPhe: 3.202 ± 0.505
7.144AlaGly: 7.144 ± 0.776
1.971AlaHis: 1.971 ± 0.469
6.487AlaIle: 6.487 ± 0.606
5.42AlaLys: 5.42 ± 1.088
7.226AlaLeu: 7.226 ± 0.892
4.106AlaMet: 4.106 ± 0.63
4.927AlaAsn: 4.927 ± 0.614
1.56AlaPro: 1.56 ± 0.372
4.681AlaGln: 4.681 ± 0.681
6.159AlaArg: 6.159 ± 0.998
6.159AlaSer: 6.159 ± 0.97
5.255AlaThr: 5.255 ± 0.888
5.748AlaVal: 5.748 ± 0.809
1.396AlaTrp: 1.396 ± 0.377
2.874AlaTyr: 2.874 ± 0.459
0.0AlaXaa: 0.0 ± 0.0
Cys
1.232CysAla: 1.232 ± 0.382
0.246CysCys: 0.246 ± 0.15
0.903CysAsp: 0.903 ± 0.281
0.739CysGlu: 0.739 ± 0.258
0.082CysPhe: 0.082 ± 0.09
1.232CysGly: 1.232 ± 0.414
0.328CysHis: 0.328 ± 0.204
0.739CysIle: 0.739 ± 0.289
0.985CysLys: 0.985 ± 0.312
0.739CysLeu: 0.739 ± 0.266
0.411CysMet: 0.411 ± 0.179
0.657CysAsn: 0.657 ± 0.246
0.328CysPro: 0.328 ± 0.175
0.082CysGln: 0.082 ± 0.09
0.739CysArg: 0.739 ± 0.351
0.821CysSer: 0.821 ± 0.29
0.575CysThr: 0.575 ± 0.256
0.493CysVal: 0.493 ± 0.229
0.246CysTrp: 0.246 ± 0.159
0.082CysTyr: 0.082 ± 0.089
0.0CysXaa: 0.0 ± 0.0
Asp
6.405AspAla: 6.405 ± 0.712
0.411AspCys: 0.411 ± 0.181
3.202AspAsp: 3.202 ± 0.528
4.352AspGlu: 4.352 ± 0.717
1.889AspPhe: 1.889 ± 0.487
5.255AspGly: 5.255 ± 0.954
0.328AspHis: 0.328 ± 0.162
2.874AspIle: 2.874 ± 0.433
2.956AspLys: 2.956 ± 0.475
5.173AspLeu: 5.173 ± 0.618
1.232AspMet: 1.232 ± 0.342
3.285AspAsn: 3.285 ± 0.553
1.478AspPro: 1.478 ± 0.37
1.642AspGln: 1.642 ± 0.336
3.202AspArg: 3.202 ± 0.611
3.367AspSer: 3.367 ± 0.597
2.874AspThr: 2.874 ± 0.606
4.27AspVal: 4.27 ± 0.72
0.493AspTrp: 0.493 ± 0.195
2.71AspTyr: 2.71 ± 0.424
0.0AspXaa: 0.0 ± 0.0
Glu
6.405GluAla: 6.405 ± 1.505
0.821GluCys: 0.821 ± 0.392
2.217GluAsp: 2.217 ± 0.402
5.666GluGlu: 5.666 ± 1.345
2.463GluPhe: 2.463 ± 0.309
3.942GluGly: 3.942 ± 0.533
0.821GluHis: 0.821 ± 0.282
4.106GluIle: 4.106 ± 0.615
4.598GluLys: 4.598 ± 0.683
5.255GluLeu: 5.255 ± 0.638
1.314GluMet: 1.314 ± 0.378
3.038GluAsn: 3.038 ± 0.518
1.889GluPro: 1.889 ± 0.385
4.106GluGln: 4.106 ± 0.642
4.763GluArg: 4.763 ± 1.078
4.024GluSer: 4.024 ± 0.513
2.546GluThr: 2.546 ± 0.506
4.106GluVal: 4.106 ± 0.471
0.985GluTrp: 0.985 ± 0.349
1.56GluTyr: 1.56 ± 0.345
0.0GluXaa: 0.0 ± 0.0
Phe
3.202PheAla: 3.202 ± 0.567
0.575PheCys: 0.575 ± 0.206
2.217PheAsp: 2.217 ± 0.451
1.642PheGlu: 1.642 ± 0.296
0.657PhePhe: 0.657 ± 0.242
2.874PheGly: 2.874 ± 0.423
0.411PheHis: 0.411 ± 0.163
2.135PheIle: 2.135 ± 0.525
1.232PheLys: 1.232 ± 0.35
1.807PheLeu: 1.807 ± 0.468
0.575PheMet: 0.575 ± 0.205
1.889PheAsn: 1.889 ± 0.316
1.478PhePro: 1.478 ± 0.317
1.15PheGln: 1.15 ± 0.341
1.724PheArg: 1.724 ± 0.453
2.628PheSer: 2.628 ± 0.455
2.217PheThr: 2.217 ± 0.428
1.642PheVal: 1.642 ± 0.283
0.575PheTrp: 0.575 ± 0.212
1.067PheTyr: 1.067 ± 0.266
0.0PheXaa: 0.0 ± 0.0
Gly
5.994GlyAla: 5.994 ± 0.777
0.739GlyCys: 0.739 ± 0.296
4.352GlyAsp: 4.352 ± 0.674
3.777GlyGlu: 3.777 ± 0.481
3.038GlyPhe: 3.038 ± 0.586
5.83GlyGly: 5.83 ± 0.694
1.067GlyHis: 1.067 ± 0.255
3.531GlyIle: 3.531 ± 0.548
3.942GlyLys: 3.942 ± 0.467
5.912GlyLeu: 5.912 ± 0.765
2.71GlyMet: 2.71 ± 0.551
4.598GlyAsn: 4.598 ± 0.927
1.314GlyPro: 1.314 ± 0.376
3.367GlyGln: 3.367 ± 0.483
3.613GlyArg: 3.613 ± 0.593
4.024GlySer: 4.024 ± 0.549
5.009GlyThr: 5.009 ± 0.777
5.42GlyVal: 5.42 ± 0.694
1.067GlyTrp: 1.067 ± 0.293
2.135GlyTyr: 2.135 ± 0.381
0.0GlyXaa: 0.0 ± 0.0
His
1.314HisAla: 1.314 ± 0.413
0.164HisCys: 0.164 ± 0.137
1.067HisAsp: 1.067 ± 0.325
1.067HisGlu: 1.067 ± 0.364
0.328HisPhe: 0.328 ± 0.208
1.15HisGly: 1.15 ± 0.311
0.411HisHis: 0.411 ± 0.152
0.985HisIle: 0.985 ± 0.307
1.067HisLys: 1.067 ± 0.36
0.739HisLeu: 0.739 ± 0.28
0.082HisMet: 0.082 ± 0.093
0.328HisAsn: 0.328 ± 0.134
0.903HisPro: 0.903 ± 0.299
0.739HisGln: 0.739 ± 0.268
1.396HisArg: 1.396 ± 0.323
0.985HisSer: 0.985 ± 0.294
0.493HisThr: 0.493 ± 0.183
0.575HisVal: 0.575 ± 0.265
0.328HisTrp: 0.328 ± 0.174
0.246HisTyr: 0.246 ± 0.126
0.0HisXaa: 0.0 ± 0.0
Ile
5.666IleAla: 5.666 ± 0.608
0.575IleCys: 0.575 ± 0.275
4.352IleAsp: 4.352 ± 0.643
3.449IleGlu: 3.449 ± 0.646
1.971IlePhe: 1.971 ± 0.441
3.449IleGly: 3.449 ± 0.612
0.821IleHis: 0.821 ± 0.27
3.202IleIle: 3.202 ± 0.555
2.463IleLys: 2.463 ± 0.463
4.106IleLeu: 4.106 ± 0.583
1.396IleMet: 1.396 ± 0.444
3.531IleAsn: 3.531 ± 0.517
2.299IlePro: 2.299 ± 0.469
2.792IleGln: 2.792 ± 0.593
3.859IleArg: 3.859 ± 0.536
4.681IleSer: 4.681 ± 0.614
3.367IleThr: 3.367 ± 0.491
2.874IleVal: 2.874 ± 0.565
1.067IleTrp: 1.067 ± 0.332
1.56IleTyr: 1.56 ± 0.38
0.0IleXaa: 0.0 ± 0.0
Lys
7.144LysAla: 7.144 ± 1.214
0.657LysCys: 0.657 ± 0.325
3.285LysAsp: 3.285 ± 0.646
4.188LysGlu: 4.188 ± 0.737
1.314LysPhe: 1.314 ± 0.369
3.038LysGly: 3.038 ± 0.45
0.493LysHis: 0.493 ± 0.212
2.792LysIle: 2.792 ± 0.534
3.367LysLys: 3.367 ± 0.811
4.188LysLeu: 4.188 ± 0.678
1.889LysMet: 1.889 ± 0.428
2.299LysAsn: 2.299 ± 0.421
3.942LysPro: 3.942 ± 0.829
3.777LysGln: 3.777 ± 0.717
2.956LysArg: 2.956 ± 0.736
3.202LysSer: 3.202 ± 0.643
4.106LysThr: 4.106 ± 0.529
3.038LysVal: 3.038 ± 0.529
0.739LysTrp: 0.739 ± 0.284
1.889LysTyr: 1.889 ± 0.421
0.0LysXaa: 0.0 ± 0.0
Leu
7.883LeuAla: 7.883 ± 0.79
1.314LeuCys: 1.314 ± 0.378
4.27LeuAsp: 4.27 ± 0.475
5.091LeuGlu: 5.091 ± 0.683
1.724LeuPhe: 1.724 ± 0.405
4.27LeuGly: 4.27 ± 0.741
1.15LeuHis: 1.15 ± 0.389
5.912LeuIle: 5.912 ± 0.707
5.173LeuLys: 5.173 ± 0.737
5.502LeuLeu: 5.502 ± 0.637
1.971LeuMet: 1.971 ± 0.438
4.188LeuAsn: 4.188 ± 0.586
2.628LeuPro: 2.628 ± 0.458
2.956LeuGln: 2.956 ± 0.545
5.83LeuArg: 5.83 ± 0.767
5.83LeuSer: 5.83 ± 0.816
5.173LeuThr: 5.173 ± 0.735
4.434LeuVal: 4.434 ± 0.543
0.575LeuTrp: 0.575 ± 0.217
1.889LeuTyr: 1.889 ± 0.327
0.0LeuXaa: 0.0 ± 0.0
Met
3.449MetAla: 3.449 ± 0.613
0.411MetCys: 0.411 ± 0.199
1.642MetAsp: 1.642 ± 0.346
0.985MetGlu: 0.985 ± 0.357
0.575MetPhe: 0.575 ± 0.28
1.724MetGly: 1.724 ± 0.303
0.246MetHis: 0.246 ± 0.129
0.985MetIle: 0.985 ± 0.306
1.971MetLys: 1.971 ± 0.308
2.463MetLeu: 2.463 ± 0.466
0.493MetMet: 0.493 ± 0.172
1.067MetAsn: 1.067 ± 0.318
1.314MetPro: 1.314 ± 0.268
1.15MetGln: 1.15 ± 0.273
1.724MetArg: 1.724 ± 0.332
2.381MetSer: 2.381 ± 0.417
1.971MetThr: 1.971 ± 0.305
0.739MetVal: 0.739 ± 0.287
0.328MetTrp: 0.328 ± 0.185
0.821MetTyr: 0.821 ± 0.223
0.0MetXaa: 0.0 ± 0.0
Asn
5.584AsnAla: 5.584 ± 0.574
0.328AsnCys: 0.328 ± 0.188
2.053AsnAsp: 2.053 ± 0.318
2.71AsnGlu: 2.71 ± 0.452
1.314AsnPhe: 1.314 ± 0.38
4.763AsnGly: 4.763 ± 0.632
0.985AsnHis: 0.985 ± 0.344
3.038AsnIle: 3.038 ± 0.531
2.628AsnLys: 2.628 ± 0.489
3.12AsnLeu: 3.12 ± 0.573
0.739AsnMet: 0.739 ± 0.212
2.874AsnAsn: 2.874 ± 0.429
2.546AsnPro: 2.546 ± 0.556
2.628AsnGln: 2.628 ± 0.401
2.299AsnArg: 2.299 ± 0.535
2.792AsnSer: 2.792 ± 0.478
2.956AsnThr: 2.956 ± 0.611
2.299AsnVal: 2.299 ± 0.563
0.821AsnTrp: 0.821 ± 0.231
1.807AsnTyr: 1.807 ± 0.394
0.0AsnXaa: 0.0 ± 0.0
Pro
3.367ProAla: 3.367 ± 0.618
0.575ProCys: 0.575 ± 0.207
1.15ProAsp: 1.15 ± 0.372
2.381ProGlu: 2.381 ± 0.431
1.56ProPhe: 1.56 ± 0.368
2.628ProGly: 2.628 ± 0.423
0.575ProHis: 0.575 ± 0.201
1.232ProIle: 1.232 ± 0.438
2.053ProLys: 2.053 ± 0.466
3.12ProLeu: 3.12 ± 0.724
0.903ProMet: 0.903 ± 0.261
1.15ProAsn: 1.15 ± 0.332
1.15ProPro: 1.15 ± 0.339
1.889ProGln: 1.889 ± 0.446
2.135ProArg: 2.135 ± 0.371
3.202ProSer: 3.202 ± 0.618
1.889ProThr: 1.889 ± 0.427
3.367ProVal: 3.367 ± 0.55
0.493ProTrp: 0.493 ± 0.233
1.067ProTyr: 1.067 ± 0.25
0.0ProXaa: 0.0 ± 0.0
Gln
5.584GlnAla: 5.584 ± 0.994
0.493GlnCys: 0.493 ± 0.16
1.56GlnAsp: 1.56 ± 0.351
2.299GlnGlu: 2.299 ± 0.371
0.985GlnPhe: 0.985 ± 0.242
2.135GlnGly: 2.135 ± 0.408
0.493GlnHis: 0.493 ± 0.186
2.463GlnIle: 2.463 ± 0.344
3.202GlnLys: 3.202 ± 0.529
4.106GlnLeu: 4.106 ± 0.541
1.396GlnMet: 1.396 ± 0.354
2.546GlnAsn: 2.546 ± 0.637
1.642GlnPro: 1.642 ± 0.313
2.71GlnGln: 2.71 ± 0.559
3.12GlnArg: 3.12 ± 0.678
3.613GlnSer: 3.613 ± 0.628
2.956GlnThr: 2.956 ± 0.507
3.202GlnVal: 3.202 ± 0.528
0.903GlnTrp: 0.903 ± 0.327
1.15GlnTyr: 1.15 ± 0.363
0.0GlnXaa: 0.0 ± 0.0
Arg
4.763ArgAla: 4.763 ± 0.729
0.739ArgCys: 0.739 ± 0.308
3.942ArgAsp: 3.942 ± 0.553
4.927ArgGlu: 4.927 ± 1.024
1.971ArgPhe: 1.971 ± 0.393
3.038ArgGly: 3.038 ± 0.417
1.067ArgHis: 1.067 ± 0.354
3.531ArgIle: 3.531 ± 0.69
4.927ArgLys: 4.927 ± 0.793
5.748ArgLeu: 5.748 ± 0.721
1.56ArgMet: 1.56 ± 0.368
3.202ArgAsn: 3.202 ± 0.581
1.478ArgPro: 1.478 ± 0.352
3.12ArgGln: 3.12 ± 0.613
3.695ArgArg: 3.695 ± 0.831
3.038ArgSer: 3.038 ± 0.529
3.449ArgThr: 3.449 ± 0.482
3.695ArgVal: 3.695 ± 0.649
1.889ArgTrp: 1.889 ± 0.438
2.053ArgTyr: 2.053 ± 0.389
0.0ArgXaa: 0.0 ± 0.0
Ser
6.323SerAla: 6.323 ± 0.78
0.575SerCys: 0.575 ± 0.194
4.106SerAsp: 4.106 ± 0.639
3.531SerGlu: 3.531 ± 0.645
2.217SerPhe: 2.217 ± 0.427
5.83SerGly: 5.83 ± 0.704
0.903SerHis: 0.903 ± 0.278
3.285SerIle: 3.285 ± 0.647
3.449SerLys: 3.449 ± 0.763
6.323SerLeu: 6.323 ± 0.766
2.135SerMet: 2.135 ± 0.478
2.463SerAsn: 2.463 ± 0.447
2.71SerPro: 2.71 ± 0.514
4.024SerGln: 4.024 ± 0.579
4.106SerArg: 4.106 ± 0.512
5.009SerSer: 5.009 ± 1.07
3.613SerThr: 3.613 ± 0.626
5.912SerVal: 5.912 ± 0.869
1.232SerTrp: 1.232 ± 0.274
0.821SerTyr: 0.821 ± 0.243
0.0SerXaa: 0.0 ± 0.0
Thr
6.241ThrAla: 6.241 ± 0.987
0.575ThrCys: 0.575 ± 0.219
3.695ThrAsp: 3.695 ± 0.46
3.367ThrGlu: 3.367 ± 0.509
2.71ThrPhe: 2.71 ± 0.546
5.337ThrGly: 5.337 ± 0.831
0.575ThrHis: 0.575 ± 0.188
3.942ThrIle: 3.942 ± 0.518
3.367ThrLys: 3.367 ± 0.454
3.777ThrLeu: 3.777 ± 0.404
1.067ThrMet: 1.067 ± 0.23
1.971ThrAsn: 1.971 ± 0.444
2.956ThrPro: 2.956 ± 0.618
1.889ThrGln: 1.889 ± 0.45
2.71ThrArg: 2.71 ± 0.467
4.27ThrSer: 4.27 ± 0.493
2.628ThrThr: 2.628 ± 0.56
4.188ThrVal: 4.188 ± 0.417
1.15ThrTrp: 1.15 ± 0.46
1.478ThrTyr: 1.478 ± 0.448
0.0ThrXaa: 0.0 ± 0.0
Val
6.159ValAla: 6.159 ± 0.713
0.493ValCys: 0.493 ± 0.195
3.038ValAsp: 3.038 ± 0.421
4.434ValGlu: 4.434 ± 0.462
2.463ValPhe: 2.463 ± 0.389
4.352ValGly: 4.352 ± 0.685
0.821ValHis: 0.821 ± 0.239
3.777ValIle: 3.777 ± 0.617
3.777ValLys: 3.777 ± 0.554
5.337ValLeu: 5.337 ± 0.689
1.642ValMet: 1.642 ± 0.294
2.71ValAsn: 2.71 ± 0.504
2.463ValPro: 2.463 ± 0.35
1.314ValGln: 1.314 ± 0.285
3.695ValArg: 3.695 ± 0.513
5.502ValSer: 5.502 ± 0.804
4.024ValThr: 4.024 ± 0.574
5.173ValVal: 5.173 ± 0.87
0.985ValTrp: 0.985 ± 0.316
1.642ValTyr: 1.642 ± 0.379
0.0ValXaa: 0.0 ± 0.0
Trp
0.657TrpAla: 0.657 ± 0.224
0.657TrpCys: 0.657 ± 0.283
0.985TrpAsp: 0.985 ± 0.284
0.739TrpGlu: 0.739 ± 0.223
0.657TrpPhe: 0.657 ± 0.274
1.314TrpGly: 1.314 ± 0.362
0.575TrpHis: 0.575 ± 0.221
0.903TrpIle: 0.903 ± 0.231
0.903TrpLys: 0.903 ± 0.282
1.478TrpLeu: 1.478 ± 0.423
0.493TrpMet: 0.493 ± 0.183
0.493TrpAsn: 0.493 ± 0.199
0.411TrpPro: 0.411 ± 0.176
0.575TrpGln: 0.575 ± 0.184
0.985TrpArg: 0.985 ± 0.286
0.985TrpSer: 0.985 ± 0.297
1.15TrpThr: 1.15 ± 0.416
1.314TrpVal: 1.314 ± 0.302
0.411TrpTrp: 0.411 ± 0.157
0.657TrpTyr: 0.657 ± 0.252
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.874TyrAla: 2.874 ± 0.548
0.493TyrCys: 0.493 ± 0.178
1.724TyrAsp: 1.724 ± 0.342
1.807TyrGlu: 1.807 ± 0.401
0.575TyrPhe: 0.575 ± 0.231
2.135TyrGly: 2.135 ± 0.379
0.328TyrHis: 0.328 ± 0.162
1.56TyrIle: 1.56 ± 0.311
0.821TyrLys: 0.821 ± 0.25
1.642TyrLeu: 1.642 ± 0.323
0.246TyrMet: 0.246 ± 0.169
1.067TyrAsn: 1.067 ± 0.328
1.396TyrPro: 1.396 ± 0.332
1.971TyrGln: 1.971 ± 0.407
3.12TyrArg: 3.12 ± 0.425
2.053TyrSer: 2.053 ± 0.534
1.642TyrThr: 1.642 ± 0.43
1.396TyrVal: 1.396 ± 0.342
0.657TyrTrp: 0.657 ± 0.21
0.657TyrTyr: 0.657 ± 0.262
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 63 proteins (12179 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski